Standard name
Human Ortholog
Description Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0.14 0.32 0.22 0.26 0.23 0.24 0.29 0.15 – 0.16 0.23 0.09 0.16 0.19 0.16 0.12 0.13 0.05 0.09 0.1 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.34 0.54 0 0.22 0.08 0.31 0.43 0.3 0.67 – 0.7 0.5 0.13 0.05 0.05 0.06 0 0.07 0 0 0 0 0 0.05
Nucleus 0.73 0.51 0.6 0.58 0.63 0.51 0.51 0.47 0.45 – 0.45 0.48 0.78 0.74 0.67 0.67 0.73 0.68 0.68 0.62 0.49 0.57 0.41 0.45
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.06 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.17 0 0 0 0.05 0 0.05 0 – 0 0 0.05 0.05 0.08 0.17 0.18 0.17 0.16 0.15 0.26 0.28 0.37 0.32
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 1 0 0 1 3 1
Bud 1 0 0 1 1 2 5 8 11 – 10 12 0 0 1 1 0 1 0 1 3 7 10 16
Bud Neck 0 0 13 3 8 1 7 7 4 – 9 8 0 0 0 0 0 0 0 0 1 0 6 5
Bud Site 0 0 0 1 0 3 8 6 10 – 9 9 0 1 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 7 12 66 42 77 76 99 116 41 – 61 72 31 58 86 90 47 40 7 10 26 5 19 18
Endoplasmic Reticulum 0 0 1 0 2 0 3 0 1 – 0 0 4 1 1 22 11 10 0 0 3 1 3 4
Endosome 0 0 0 5 1 4 2 1 4 – 0 2 4 11 20 7 8 14 1 3 2 1 5 4
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 1 2 0 1 0 1 1
Mitochondria 43 47 4 42 25 100 177 122 186 – 265 158 43 18 24 35 2 20 1 2 7 1 18 21
Nucleus 93 44 124 111 188 166 211 193 126 – 170 153 257 269 311 369 273 205 102 70 132 105 190 205
Nuclear Periphery 0 0 0 0 0 0 0 1 2 – 0 0 0 0 1 0 0 0 0 1 1 0 0 0
Nucleolus 1 1 1 0 0 0 0 0 0 – 0 0 5 0 2 2 0 1 0 0 0 0 8 4
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 1 3 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 3 8 12 8 12 23 2 – 3 10 0 6 4 4 0 1 6 4 8 3 14 17
Vac/Vac Membrane 13 15 9 2 10 17 8 19 12 – 9 13 18 18 36 95 66 52 25 16 70 51 174 143
Unique Cell Count 128 87 208 190 299 324 414 407 277 376 318 331 363 463 551 376 302 152 114 269 185 468 452
Labelled Cell Count 158 119 221 215 324 377 534 496 399 536 437 362 383 491 626 407 346 152 114 269 185 468 452


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 3.8 5.0 4.4 5.0 4.1 4.1 4.4 3.7 – 3.4 4.0 4.8 5.0 4.9 6.9 7.2 6.9 5.2 4.5 5.2
Std Deviation (1e-4) 0.6 0.6 1.2 1.4 1.3 1.4 1.0 1.2 1.5 – 1.2 1.1 1.6 1.7 1.3 1.3 1.6 1.5 9.2 1.7 5.7
Intensity Change (Log2) – – – -0.18 -0.01 -0.3 -0.31 -0.19 -0.45 – -0.55 -0.33 -0.07 -0.01 -0.04 0.46 0.51 0.46 0.05 -0.16 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 4.6 4.6 4.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1926 1.8152 1.6202 1.9017 1.2347 1.5629 -0.7834 -0.4788 -0.52 -0.4963 -0.4382 -0.5519 -0.3901 -0.1015 -0.3089 0.0021 -0.6978 -0.3573
Actin 0.1365 0.012 0.0081 0.0229 0.1049 0.0016 0.0186 0.0004 0.0086 0.0028 0.0065 0.0002 0.0298 0.0003 0.0124 0.0001 0.0023 0.004
Bud 0.0007 0.0002 0.0001 0.0006 0.0006 0.0001 0.0006 0.0002 0.0005 0.0009 0.0003 0.0001 0.0023 0.0014 0.0008 0.0001 0.0001 0.0002
Bud Neck 0.0162 0.0009 0.0017 0.0006 0.0054 0.0036 0.0017 0.0004 0.0011 0.004 0.0031 0.0022 0.0076 0.0004 0.0013 0.0025 0.0006 0.0026
Bud Periphery 0.0009 0.0001 0.0001 0.001 0.0012 0.0001 0.0007 0.0003 0.0007 0.0011 0.0003 0.0001 0.0018 0.0006 0.001 0 0.0001 0.0002
Bud Site 0.0091 0.0014 0.0035 0.0011 0.0023 0.0002 0.0042 0.0007 0.0043 0.003 0.0012 0.0001 0.004 0.0043 0.0044 0.0005 0.0002 0.0002
Cell Periphery 0.0008 0.0002 0.0002 0.0001 0.0007 0.0001 0.0003 0 0.0003 0.0002 0.0001 0 0.0004 0.0001 0.0002 0.0001 0 0.0001
Cytoplasm 0.1086 0.0998 0.0553 0.0178 0.0121 0.0667 0.0471 0.0726 0.0652 0.0578 0.0417 0.0258 0.04 0.0603 0.0355 0.0107 0.0562 0.02
Cytoplasmic Foci 0.0454 0.0075 0.0316 0.0039 0.0066 0.0122 0.0189 0.0061 0.0134 0.0134 0.0497 0.0016 0.0181 0.0035 0.0078 0.0073 0.0038 0.0042
Eisosomes 0.0017 0.0001 0.0002 0.0007 0.0013 0 0.0003 0 0.0001 0 0.0001 0 0.0005 0.0001 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0096 0.0093 0.0025 0.0009 0.0041 0.0029 0.0033 0.0032 0.0035 0.0022 0.0014 0.0012 0.0043 0.0021 0.0015 0.0002 0.0023 0.0022
Endosome 0.028 0.0073 0.0198 0.0051 0.0129 0.0108 0.0579 0.0068 0.013 0.0402 0.0465 0.0025 0.0225 0.0017 0.0084 0.0152 0.0064 0.0054
Golgi 0.0167 0.0079 0.0059 0.009 0.0096 0.0092 0.0094 0.0005 0.0041 0.0209 0.0093 0.0002 0.0119 0.0001 0.0056 0.0018 0.0041 0.002
Lipid Particles 0.0378 0.0017 0.0139 0.0056 0.0319 0.0104 0.0111 0.0005 0.002 0.0036 0.0275 0.0002 0.0115 0.0003 0.0047 0.0037 0.019 0.0068
Mitochondria 0.0037 0.0016 0.0015 0.1937 0.0074 0.0325 0.0126 0.0035 0.0061 0.0073 0.0043 0.0102 0.0095 0.0008 0.0163 0.0008 0.0016 0.0051
None 0.2441 0.2138 0.2287 0.1557 0.0529 0.0996 0.0585 0.0907 0.0312 0.0388 0.0573 0.012 0.0572 0.0793 0.0509 0.1036 0.1229 0.0203
Nuclear Periphery 0.016 0.0262 0.0122 0.0192 0.0069 0.0136 0.0228 0.0375 0.0274 0.0321 0.0118 0.0192 0.024 0.0145 0.0183 0.0054 0.0254 0.0169
Nucleolus 0.0153 0.0139 0.031 0.0077 0.0135 0.0101 0.0042 0.003 0.0031 0.0084 0.0058 0.0093 0.006 0.0051 0.0061 0.0033 0.0041 0.0093
Nucleus 0.241 0.5395 0.5107 0.5169 0.7017 0.6576 0.6857 0.7549 0.7785 0.7159 0.614 0.882 0.6929 0.7462 0.7439 0.7464 0.69 0.8328
Peroxisomes 0.0239 0.0025 0.0067 0.0092 0.0035 0.0011 0.0034 0.0003 0.0025 0.0076 0.0371 0.0002 0.0083 0.0001 0.0062 0.0021 0.0045 0.0023
Punctate Nuclear 0.0395 0.0497 0.0636 0.0249 0.0152 0.0659 0.0258 0.0158 0.029 0.0266 0.0778 0.0312 0.0394 0.0772 0.0721 0.0923 0.0546 0.064
Vacuole 0.0035 0.0038 0.0025 0.0025 0.0041 0.0015 0.0083 0.0018 0.0044 0.0107 0.0032 0.0013 0.0062 0.0015 0.0017 0.0035 0.0013 0.0008
Vacuole Periphery 0.0008 0.0006 0.0004 0.0012 0.0013 0.0003 0.0049 0.0008 0.0011 0.0023 0.0011 0.0004 0.0018 0.0002 0.0009 0.0004 0.0004 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.8716 8.4601 4.2803 6.0484 3.0676 3.4395 6.3183 7.0855 6.6438 5.9463
Translational Efficiency 1.2019 0.4097 1.2116 0.8426 1.3701 1.7259 0.8069 0.74 0.8395 0.7633

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1286 612 167 1135 1317 1424 241 24 2603 2036 408 1159

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 636.36 762.06 1035.12 832.06 636.32 758.02 907.52 874.58 636.34 759.23 959.75 832.94
Standard Deviation 64.72 96.83 131.98 115.22 94.24 99.49 95.37 180.67 81.01 98.71 128.21 117.10
Intensity Change Log 2 — 0.260061 0.701883 0.386844 — 0.252483 0.512177 0.458838 — 0.256278 0.610149 0.423289

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000119 0.000301 0.002145 0.001332 0.000111 0.000459 0.000335 0.000944 0.000115 0.000412 0.001076 0.001324
Bud Neck 0.004378 0.006152 0.008338 0.013931 0.003000 0.008896 0.001353 0.007151 0.003681 0.008071 0.004212 0.013790
Bud Site 0.001487 0.003559 0.008962 0.016920 0.001216 0.004725 0.000734 0.019466 0.001350 0.004375 0.004102 0.016973
Cell Periphery 0.000143 0.000154 0.000202 0.000300 0.000162 0.000166 0.000108 0.000221 0.000153 0.000163 0.000147 0.000298
Cytoplasm 0.222081* 0.118834* 0.009523 0.137934* 0.169333* 0.127579* 0.029619 0.041660 0.195393* 0.124950* 0.021393 0.135940*
Cytoplasmic Foci 0.034052 0.036441 0.000441 0.012384 0.018732 0.030965 0.003810 0.000211 0.026301 0.032611 0.002431 0.012132
Eisosomes 0.000035 0.000055 0.000084 0.000055 0.000031 0.000041 0.000062 0.000026 0.000033 0.000045 0.000071 0.000054
Endoplasmic Reticulum 0.000667 0.003020 0.018028 0.007477 0.001542 0.002153 0.004883 0.005138 0.001110 0.002413 0.010263 0.007429
Endosome 0.000986 0.002868 0.001164 0.011705 0.001342 0.003287 0.001027 0.000525 0.001167 0.003161 0.001083 0.011473
Golgi 0.000382 0.001866 0.000152 0.005601 0.000191 0.001851 0.000054 0.000055 0.000285 0.001855 0.000094 0.005486
Lipid Particles 0.003118 0.005495 0.001180 0.004531 0.002675 0.004742 0.001604 0.000378 0.002894 0.004968 0.001431 0.004445
Mitochondria 0.003449 0.007646 0.001306 0.007908 0.002365 0.005967 0.000487 0.001398 0.002900 0.006472 0.000822 0.007773
Mitotic Spindle 0.002014 0.004106 0.001224 0.053225 0.001557 0.008647 0.000496 0.016180 0.001783 0.007282 0.000794 0.052458
None 0.009619 0.006445 0.002884 0.012532 0.016906 0.006140 0.004899 0.007875 0.013306 0.006232 0.004074 0.012435
Nuclear Periphery 0.001033 0.001785 0.001471 0.003535 0.001269 0.002580 0.001188 0.002466 0.001152 0.002341 0.001304 0.003512
Nuclear Periphery Foci 0.000209 0.000930 0.000667 0.001873 0.000987 0.000585 0.000487 0.000327 0.000602 0.000689 0.000561 0.001841
Nucleolus 0.003409 0.005061 0.003203 0.001633 0.004746 0.004992 0.002608 0.001052 0.004085 0.005013 0.002851 0.001621
Nucleus 0.705693* 0.780733* 0.919593* 0.651221* 0.768749* 0.773207* 0.930052* 0.877634* 0.737597* 0.775469* 0.925771* 0.655910*
Peroxisomes 0.002733 0.004833 0.000409 0.003157 0.001032 0.003842 0.000370 0.000089 0.001872 0.004140 0.000386 0.003094
Vacuole 0.004011 0.008663 0.018803 0.047204 0.003740 0.008181 0.015626 0.017063 0.003874 0.008326 0.016926 0.046580
Vacuole Periphery 0.000382 0.001053 0.000222 0.005545 0.000315 0.000994 0.000200 0.000141 0.000348 0.001011 0.000209 0.005433

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.56 -9.73 -5.05 -4.33 4.63 -2.02 -6.75 -3.65 -3.19 -3.15 -2.41 -9.99 -5.43 -3.86 0.71
Bud Neck -1.73 -6.48 -7.79 -6.25 -1.99 -5.15 1.48 -2.89 -1.60 -3.05 -4.94 -4.27 -9.21 -5.25 -6.88
Bud Site -1.65 -1.85 -8.16 -6.00 -1.15 -3.07 0.29 -1.83 -1.69 -1.84 -3.40 -1.77 -8.56 -6.22 -4.52
Cell Periphery -0.63 -3.28 -2.49 -2.12 0.81 -0.09 2.24 -1.26 -1.18 -2.13 -0.53 -0.98 -2.37 -1.92 -1.61
Cytoplasm 10.79 29.85 13.65 1.20 -16.86 5.26 17.66 11.05 8.01 -0.65 11.60 31.42 13.08 2.88 -14.25
Cytoplasmic Foci -0.68 15.64 11.58 7.82 -6.04 -4.34 9.00 11.65 14.04 3.75 -2.70 17.27 11.89 12.19 -4.33
Eisosomes -4.48 -7.43 -9.23 -2.38 3.91 -3.28 -5.85 -2.87 -2.41 -0.90 -5.11 -9.25 -10.57 -6.45 2.59
Endoplasmic Reticulum -10.59 -13.70 -17.54 -12.38 6.87 -2.63 -7.31 -3.36 -3.28 -2.51 -8.13 -14.09 -17.03 -14.37 2.26
Endosome -4.14 -2.59 -5.34 -3.05 -4.28 -3.04 0.51 -0.91 1.81 -1.32 -4.54 -1.06 -5.11 -2.79 -4.81
Golgi -1.95 1.09 -2.89 -0.51 -3.27 -3.04 3.15 -0.21 3.00 -2.57 -3.47 2.10 -3.13 -0.58 -3.45
Lipid Particles -3.02 3.17 0.37 2.65 -1.23 -3.60 2.89 4.33 6.70 1.37 -4.53 4.28 0.21 2.58 -1.29
Mitochondria -1.84 1.69 -0.88 1.33 -3.29 -3.69 2.89 -1.24 1.17 -2.66 -3.58 3.09 -1.63 1.55 -4.07
Mitotic Spindle -1.17 -0.97 -8.16 -7.24 -7.15 -3.39 1.12 -2.29 -2.04 -2.32 -3.51 -0.33 -8.51 -6.97 -8.34
None 2.75 6.60 1.05 -1.29 -4.62 6.63 6.96 3.38 0.07 -0.39 6.99 8.99 3.64 -1.53 -3.46
Nuclear Periphery -4.04 -5.35 -10.41 -8.11 -7.16 -6.46 -1.85 -2.83 -2.51 -2.76 -7.78 -5.13 -10.66 -7.23 -8.81
Nuclear Periphery Foci -3.21 -2.76 -5.83 -2.42 -2.22 0.99 1.03 -1.24 -1.53 -1.53 -0.33 -0.58 -3.77 -3.77 -3.70
Nucleolus -0.58 1.46 2.05 3.03 0.73 0.82 3.23 3.20 2.94 0.21 0.21 3.47 3.60 3.82 0.30
Nucleus -5.42 -12.82 1.50 6.32 13.22 -0.47 -13.15 1.06 1.15 3.68 -4.53 -18.88 4.55 7.75 18.61
Peroxisomes -1.76 3.09 1.39 3.03 -1.87 -4.80 3.51 3.89 6.66 0.33 -3.69 4.05 0.66 3.77 -2.12
Vacuole -5.18 -5.63 -16.90 -15.32 -5.57 -5.55 -4.79 -2.98 -2.75 -1.99 -7.23 -7.32 -17.19 -15.84 -8.67
Vacuole Periphery -1.83 0.46 -3.25 -2.02 -3.58 -3.63 1.32 -0.57 3.14 -1.63 -3.59 1.02 -3.43 -2.24 -3.67
Standard name
Human Ortholog
Description Protein required for DNA repair; component of the Mre11 complex, which is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling
Localization
Cell Percentages nucleus (69%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Xrs2

Xrs2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Xrs2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available