Standard name
Human Ortholog
Description Farnesyl cysteine-carboxyl methyltransferase; mediates the carboxyl methylation step during C-terminal CAAX motif processing of a-factor and RAS proteins in the endoplasmic reticulum, localizes to the ER membrane
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Mutation Strain ID Temperature Mean Single Mutant Fitness Single Mutant Fitness SD Penetrance Results Specific Phenotype Results
ste14Δ DMA1137 30 — —
compartment (FP-tagged protein)# cells# replicatesmean penetrancepenetrance SDpenetrance mutantimage
Actin patch (Sac6)11127.3—no
Cortical patch (Sla1)13138.5—no
Late endosome (Snf7)—————

—

Vacuole (Vph1)—————

—
phenotypemeanSDSPMdiagram
Actin patchActin patch
Actin patch (Sac6)aggregate0—no
Actin patch (Sac6)bright patches18.2—no
Actin patch (Sac6)decreased patch number9.1—no
Actin patch (Sac6)depolarized patches0—no
Actin patch (Sac6)WT72.7NANA
Cortical patchCortical patch
Cortical patch (Sla1)aggregate7.7—no
Cortical patch (Sla1)decreased patch number23.1—no
Cortical patch (Sla1)depolarized patches0—no
Cortical patch (Sla1)increased patch number7.7—no
Cortical patch (Sla1)WT61.5NANA
Late endosomeLate endosome
Late endosome (Snf7)fragmented———
Late endosome (Snf7)condensed———
Late endosome (Snf7)membrane **———
Late endosome (Snf7)WT—NANA
VacuoleVacuole
Vacuole (Vph1)class E———
Vacuole (Vph1)enlarged———
Vacuole (Vph1)fragmented———
Vacuole (Vph1)multilobed———
Vacuole (Vph1)class G———
Vacuole (Vph1)V-ATPase defect———
Vacuole (Vph1)WT—NANA
penetrance = percentage of the population with an aberrant phenotype

FP = fluorescent protein

SD = standard deviation

* temperature-sentitive strains imaged at room temperature (RT) were analyzed but not scored

specific phenotype mutant (SPM)
  • yes+ = stringent SPM

** membrane = localization of Snf7 to the vacuolar membrane

NA = not applicable

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0.05 0 0 0 0 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.69 0.76 0.82 0.84 0.88 0.85 0.88 0.83 0.82 0.83 0.79 0.63 0.53 0.57 0.87 0.86 0.84 0.21 0.27 0.11 0.11 0.13 0.14
Endoplasmic Reticulum – 0.27 0.22 0.19 0.11 0.05 0 0 0 0 0 0.05 0.37 0.47 0.46 0 0 0.05 0.43 0.44 0.64 0.52 0.47 0.41
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.05 0.07 0.07
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0 0.09 0.06 0.12 0.11 0.18 0.21 0 0 0 0.05 0 0 0.12 0 0.06 0.05 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0.05 0.09 0.07 0.13 0.16 0.13 0.21 0.21 0.29
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 2 2 1 3 2 2 4 6 2 0 8 3 19 9 6 6 5 0 2 3 4 10 7
Bud – 2 2 1 3 14 17 22 15 16 20 24 0 3 2 1 1 0 0 0 0 3 5 6
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 2 3 5
Bud Site – 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 5 1 6 4 1 5 2 5 7 8 15 3 4 0 0 0 0 0 0 0
Cytoplasm – 86 110 163 305 263 453 523 338 443 254 379 181 336 362 239 182 147 5 39 19 33 64 84
Endoplasmic Reticulum – 34 32 37 40 16 14 19 11 18 8 25 105 299 291 10 4 9 11 65 113 163 239 251
Endosome – 3 0 4 13 4 9 8 11 13 1 3 0 5 1 5 8 11 0 3 3 15 37 45
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 3 7 7
Mitochondria – 1 2 1 5 7 47 35 47 58 56 99 0 0 0 13 6 1 3 5 10 17 21 17
Nucleus – 1 0 0 4 0 1 3 0 2 0 1 2 0 2 0 3 1 0 0 0 0 1 1
Nuclear Periphery – 0 0 0 0 1 2 2 0 1 0 0 0 1 0 0 0 0 0 1 0 2 3 5
Nucleolus – 1 0 0 0 1 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 3 0 0 0 0 0 1 0 1 0 1 0 0 0 1
Vac/Vac Membrane – 1 1 1 9 10 27 18 26 22 10 12 2 5 3 13 20 12 3 23 23 67 107 181
Unique Cell Count 125 144 198 363 298 533 594 408 538 306 480 287 632 637 275 212 174 28 148 178 317 509 620
Labelled Cell Count 131 149 208 387 319 579 639 456 585 351 556 300 676 685 291 234 188 28 148 178 317 509 620


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 7.2 5.9 6.6 6.4 5.8 5.0 5.2 5.1 4.9 4.7 4.6 8.9 8.1 8.3 6.6 6.8 7.3 9.6 10.0 10.7
Std Deviation (1e-4) – 1.1 0.7 1.0 1.1 1.1 1.1 1.0 1.0 0.9 0.8 0.8 1.1 1.0 1.0 1.1 1.1 1.2 1.4 1.6 1.5
Intensity Change (Log2) – – – 0.16 0.1 -0.03 -0.24 -0.2 -0.23 -0.29 -0.34 -0.36 0.58 0.45 0.49 0.16 0.2 0.3 0.7 0.76 0.85

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1.7 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.4 2.0 3.2 2.4 3.6 1.7 1.6 1.7 0.7 -2.8 -5.1 -4.3 2.7 2.3 1.8
Endoplasmic Reticulum -0.8 -3.3 -5.3 -8.3 -8.1 -7.5 0 0 0 3.0 5.5 5.2 -6.0 -6.2 -4.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.1 2.2 3.7 0 0 0 0 0 0 1.7 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.3 0 2.7 0 0 0 0 0 0 0 3.4 2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.2418 6.3151 5.4903 5.6034 5.1918 5.5403 4.578 4.9851 4.7181 4.3072 4.1472 4.8338 3.3494 4.3876 3.9317 4.1968 2.983 3.7183
Actin 0.0207 0.0009 0.0027 0.01 0.0026 0.0024 0.004 0.0015 0.0028 0.0504 0.0002 0.0006 0.0033 0.0059 0.0012 0.0155 0.0022 0.0091
Bud 0.0024 0.0002 0.0003 0.0003 0.0002 0.0007 0.0002 0.0004 0.0007 0.0026 0.0002 0.0002 0.0002 0.0001 0.0003 0.0003 0.0002 0.0001
Bud Neck 0.0012 0.0009 0.0005 0.0012 0.0008 0.0013 0.0003 0.0003 0.0005 0.0012 0.0002 0.0005 0.0004 0.0015 0.0006 0.001 0.0004 0.0006
Bud Periphery 0.0018 0.0003 0.0003 0.0005 0.0002 0.0005 0.0003 0.0005 0.0007 0.0022 0.0003 0.0003 0.0005 0.0001 0.0002 0.0004 0.0003 0.0001
Bud Site 0.0037 0.0026 0.0006 0.002 0.0003 0.0003 0.0009 0.0017 0.001 0.0044 0.0002 0.0001 0.0006 0.0003 0.0005 0.0005 0.0004 0.0001
Cell Periphery 0.0005 0.001 0.0003 0.0003 0.0002 0.0002 0.001 0.002 0.0006 0.0008 0.0002 0.0004 0.0007 0.0004 0.0003 0.0004 0.0002 0.0001
Cytoplasm 0.2267 0.4214 0.2443 0.2486 0.3173 0.3356 0.1695 0.3085 0.1758 0.2563 0.1814 0.2558 0.1616 0.2885 0.1999 0.1735 0.2795 0.2062
Cytoplasmic Foci 0.0432 0.0287 0.0386 0.0197 0.0539 0.0347 0.0401 0.0316 0.0365 0.0347 0.0361 0.04 0.0282 0.0182 0.0287 0.0209 0.0164 0.0238
Eisosomes 0.0004 0 0 0.0001 0.0001 0 0.0002 0.0001 0.0001 0.0009 0 0 0 0.0001 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.3085 0.2978 0.3208 0.3928 0.1693 0.3498 0.2914 0.3103 0.2613 0.2809 0.1679 0.2642 0.3685 0.4247 0.3705 0.1975 0.2428 0.3571
Endosome 0.1965 0.1594 0.2375 0.117 0.2612 0.1568 0.2968 0.1912 0.3388 0.2051 0.4316 0.3151 0.2514 0.1481 0.2586 0.2513 0.2444 0.2852
Golgi 0.0132 0.0056 0.0179 0.0071 0.0111 0.0076 0.0234 0.015 0.0265 0.0166 0.0122 0.0221 0.0172 0.0104 0.0156 0.0208 0.0081 0.0095
Lipid Particles 0.0233 0.0129 0.0132 0.0213 0.0076 0.0085 0.0282 0.0106 0.0147 0.0086 0.0025 0.0088 0.0142 0.0074 0.0128 0.0194 0.0027 0.0076
Mitochondria 0.0129 0.0028 0.0133 0.0046 0.0047 0.0063 0.0089 0.0103 0.0101 0.0172 0.0085 0.0066 0.0047 0.001 0.0022 0.0028 0.0396 0.0027
None 0.0128 0.0048 0.0066 0.0042 0.0256 0.0146 0.0321 0.0106 0.0248 0.0229 0.0381 0.0149 0.0034 0.0032 0.0052 0.0028 0.0009 0.0082
Nuclear Periphery 0.0431 0.0176 0.0287 0.0312 0.0267 0.0258 0.0258 0.0172 0.0199 0.0182 0.0173 0.013 0.0514 0.0353 0.0373 0.0537 0.0275 0.0323
Nucleolus 0.0013 0.0001 0.0002 0.0006 0.0023 0.0007 0.0003 0.0002 0.0001 0.0002 0.0001 0.0001 0.0002 0.0002 0 0.0002 0.0001 0.0001
Nucleus 0.0093 0.0045 0.0044 0.0053 0.016 0.0077 0.0026 0.0029 0.0019 0.0021 0.0021 0.0019 0.0044 0.0036 0.0031 0.012 0.0051 0.0038
Peroxisomes 0.0138 0.0002 0.0035 0.0183 0.0015 0.0009 0.0027 0.0002 0.0022 0.0049 0.0003 0.0003 0.0008 0.0002 0.0003 0.0018 0.0006 0.0002
Punctate Nuclear 0.0062 0.0009 0.0024 0.0026 0.0223 0.0027 0.0017 0.0005 0.0016 0.001 0.0005 0.0005 0.0024 0.0022 0.0006 0.0026 0.0004 0.001
Vacuole 0.0507 0.0335 0.0546 0.1032 0.067 0.037 0.0584 0.0779 0.064 0.0529 0.0731 0.0444 0.0749 0.0439 0.052 0.1918 0.112 0.0396
Vacuole Periphery 0.0079 0.0038 0.0094 0.009 0.0094 0.0058 0.0111 0.0064 0.0155 0.0157 0.0269 0.0104 0.011 0.0049 0.0102 0.0306 0.0162 0.0125

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 28.3514 27.3993 18.2983 26.848 20.8631 13.7849 24.9331 28.0469 24.8885 31.3681
Translational Efficiency 0.8327 0.6288 0.944 0.5529 0.7827 1.9183 0.8823 0.4876 0.8732 0.6379

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1269 1483 878 630 2036 1531 2252 1788 3305 3014 3130 2418

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 743.73 958.16 1026.51 1097.82 865.26 953.97 1063.94 1114.62 818.60 956.03 1053.44 1110.24
Standard Deviation 99.80 158.12 145.80 135.88 126.82 153.56 134.72 154.23 131.24 155.84 138.94 149.85
Intensity Change Log 2 — 0.365488 0.464897 0.561791 — 0.140810 0.298211 0.365346 — 0.249025 0.377658 0.459483

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000324 0.000835 0.000989 0.009416 0.000681 0.001266 0.002757 0.008069 0.000544 0.001054 0.002261 0.008420
Bud Neck 0.002807 0.001291 0.004269 0.009565 0.001546 0.001429 0.003044 0.008124 0.002030 0.001361 0.003388 0.008500
Bud Site 0.002402 0.004501 0.009675 0.030675 0.002402 0.003837 0.011345 0.026471 0.002402 0.004164 0.010876 0.027566
Cell Periphery 0.000372 0.000424 0.000346 0.003053 0.000496 0.000883 0.001147 0.002451 0.000448 0.000657 0.000922 0.002608
Cytoplasm 0.343902 0.126550 0.129047 0.168224 0.158998 0.081632 0.135108 0.196459 0.229995 0.103734 0.133408 0.189103
Cytoplasmic Foci 0.236121 0.131020 0.103198 0.059356 0.107863 0.084390 0.054366 0.064091 0.157109 0.107334 0.068064 0.062858
Eisosomes 0.000341 0.000206 0.000058 0.001159 0.000643 0.000477 0.000342 0.001142 0.000527 0.000344 0.000262 0.001147
Endoplasmic Reticulum 0.147269 0.495285 0.213180 0.236574 0.478928 0.584587 0.524476 0.396948 0.351583 0.540647 0.437154 0.355163
Endosome 0.023595 0.046543 0.107543 0.040457 0.029925 0.047937 0.051631 0.034499 0.027494 0.047251 0.067315 0.036051
Golgi 0.016272 0.040696 0.010069 0.007393 0.063934 0.063380 0.033769 0.023285 0.045634 0.052219 0.027121 0.019144
Lipid Particles 0.099394 0.057625 0.054244 0.094486 0.088902 0.066588 0.083766 0.105050 0.092931 0.062178 0.075485 0.102298
Mitochondria 0.003687 0.003812 0.000905 0.000593 0.011354 0.006302 0.003369 0.002744 0.008411 0.005077 0.002678 0.002183
Mitotic Spindle 0.000254 0.001445 0.007698 0.008935 0.000243 0.000380 0.001323 0.003320 0.000248 0.000904 0.003112 0.004783
None 0.003342 0.002001 0.000800 0.000370 0.004231 0.001415 0.002325 0.001264 0.003890 0.001704 0.001897 0.001031
Nuclear Periphery 0.003628 0.002730 0.009700 0.002927 0.002124 0.000796 0.000670 0.000990 0.002701 0.001748 0.003203 0.001495
Nuclear Periphery Foci 0.008810 0.013154 0.064798 0.049665 0.004939 0.005050 0.011405 0.020860 0.006425 0.009037 0.026382 0.028365
Nucleolus 0.000729 0.000301 0.001268 0.000252 0.000343 0.000235 0.000165 0.000236 0.000491 0.000268 0.000474 0.000240
Nucleus 0.049538 0.005588 0.013740 0.022571 0.007989 0.002924 0.005345 0.019135 0.023942 0.004235 0.007700 0.020030
Peroxisomes 0.003444 0.001498 0.000942 0.001662 0.003191 0.004018 0.001332 0.003018 0.003288 0.002778 0.001223 0.002665
Vacuole 0.051476 0.054527 0.258178 0.251361 0.025361 0.033415 0.067538 0.078838 0.035388 0.043803 0.121014 0.123788
Vacuole Periphery 0.002293 0.009966 0.009353 0.001304 0.005906 0.009057 0.004776 0.003006 0.004519 0.009505 0.006060 0.002563

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.60 -3.28 -4.52 -4.22 -4.11 -2.85 -5.00 -8.62 -7.83 -5.72 -3.53 -5.68 -9.70 -8.98 -7.10
Bud Neck 4.11 -3.25 -8.93 -12.16 -6.85 0.46 -4.95 -11.07 -11.09 -8.03 3.26 -5.25 -13.23 -15.00 -9.96
Bud Site -2.64 -5.98 -7.76 -7.20 -5.48 -2.69 -8.80 -12.09 -11.17 -6.84 -3.94 -10.38 -14.33 -13.23 -8.70
Cell Periphery -1.38 0.83 -5.08 -4.96 -5.13 -4.03 -6.71 -10.72 -7.83 -6.43 -3.89 -6.81 -11.46 -10.04 -8.34
Cytoplasm 27.04 22.82 16.60 -4.58 -3.58 15.90 4.26 -6.38 -19.63 -9.94 27.04 19.08 6.68 -17.10 -10.39
Cytoplasmic Foci 15.46 18.99 28.32 13.20 7.51 5.17 14.31 11.16 5.09 -3.20 12.29 24.75 26.35 13.37 1.77
Eisosomes 3.79 11.50 -4.12 -4.90 -5.83 2.59 5.58 -5.06 -7.08 -9.24 4.56 7.41 -7.11 -9.66 -11.00
Endoplasmic Reticulum -31.00 -5.65 -4.54 19.64 0.12 -8.98 -3.82 8.47 16.45 12.35 -21.41 -9.28 1.50 21.31 10.06
Endosome -11.35 -19.38 -4.90 2.73 13.80 -8.02 -11.29 -2.53 5.77 8.45 -12.91 -21.27 -5.32 6.75 15.34
Golgi -11.25 5.11 6.51 14.72 2.29 0.15 11.93 16.39 13.13 5.80 -2.92 10.65 15.48 16.71 5.86
Lipid Particles 9.30 9.58 1.52 -5.14 -5.59 5.23 1.19 -3.31 -7.87 -4.38 10.00 5.42 -2.01 -10.29 -6.53
Mitochondria -0.23 5.63 6.60 8.03 1.93 3.97 7.43 8.02 4.20 1.16 4.24 8.12 8.80 5.58 1.27
Mitotic Spindle -3.14 -5.05 -3.67 -3.14 -0.50 -1.26 -3.04 -4.66 -4.45 -2.94 -3.38 -5.86 -5.83 -4.95 -2.13
None 1.61 3.39 4.33 3.46 1.41 3.31 2.17 3.62 0.34 1.69 3.57 3.14 4.85 1.68 1.87
Nuclear Periphery 1.47 -4.25 0.38 -0.59 3.99 3.15 3.52 2.58 -1.33 -2.43 2.52 -0.98 2.95 0.40 3.45
Nuclear Periphery Foci -3.27 -14.24 -11.97 -10.31 2.86 -0.14 -7.96 -11.44 -11.09 -6.24 -3.50 -15.17 -15.87 -13.15 -1.17
Nucleolus 4.79 -0.91 6.00 0.72 1.72 1.16 3.62 2.00 0.07 -3.05 3.71 0.07 5.66 0.60 1.41
Nucleus 22.03 15.23 9.37 -7.81 -3.39 6.58 3.42 -9.91 -13.71 -12.03 20.40 15.85 2.40 -15.38 -11.45
Peroxisomes 3.34 4.37 3.17 -0.35 -3.41 -1.39 9.41 0.63 1.63 -5.16 1.29 8.20 2.03 0.48 -5.78
Vacuole -0.91 -24.18 -22.16 -21.82 -2.03 -3.16 -14.52 -17.67 -13.83 -3.85 -4.00 -24.11 -24.60 -21.88 -2.90
Vacuole Periphery -12.11 -9.55 4.93 13.44 10.84 -4.85 2.19 5.76 9.34 3.41 -10.59 -3.52 5.67 14.11 7.91
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Farnesyl cysteine-carboxyl methyltransferase; mediates the carboxyl methylation step during C-terminal CAAX motif processing of a-factor and RAS proteins in the endoplasmic reticulum, localizes to the ER membrane
Localization
Cell Percentages ER (80%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Ste14

Ste14


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ste14-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available