Standard name
Human Ortholog
Description Member of the NineTeen Complex (NTC); that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; relocalizes to the cytosol in response to hypoxia; homologs in human and C. elegans
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.08 0 0.05 0.05 0.05 0 0.05 0.05 0 – 0.05 0 0.06 0.08 0.14 0.12 0.1 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.08 0.05 0.11 0.06 0.29 0.33 0.17 0.54 – 0.59 0.33 0.06 0 0 0 0 0.14 0 0 0 0 0 0
Nucleus 0.88 0.95 0.94 0.93 0.9 0.86 0.83 0.88 0.8 – 0.81 0.85 0.93 0.89 0.81 0.77 0.78 0.8 0.85 0.89 0.84 0.86 0.7 0.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.11 0.08 0.11 0 0.05 0.1 0.09 0.18 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 1 0 1
Bud 1 1 0 0 0 1 5 5 4 – 9 15 0 0 0 0 0 0 0 1 0 0 6 9
Bud Neck 0 0 1 1 1 1 1 4 1 – 2 3 0 0 0 0 0 0 0 0 0 1 3 9
Bud Site 0 0 0 1 1 6 6 5 20 – 9 16 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 13 4 15 11 26 21 31 33 15 – 16 17 29 54 87 12 9 4 4 0 2 0 1 2
Endoplasmic Reticulum 2 0 0 0 0 0 1 0 0 – 0 0 0 0 3 2 4 0 0 0 0 0 3 2
Endosome 0 0 1 0 0 0 1 0 0 – 0 1 0 2 6 0 4 1 3 0 2 0 2 8
Golgi 0 1 0 0 0 0 0 0 0 – 0 0 0 0 1 1 1 0 0 1 0 0 1 0
Mitochondria 13 15 15 23 27 147 192 118 258 – 200 151 28 11 13 4 2 9 1 1 3 0 7 19
Nucleus 138 175 309 201 438 434 489 603 386 – 273 384 474 575 504 79 72 51 136 166 297 163 379 408
Nuclear Periphery 0 0 0 0 2 1 4 4 8 – 1 2 1 4 5 1 1 0 1 0 1 0 4 4
Nucleolus 2 2 0 1 0 1 3 1 1 – 7 4 4 6 8 0 0 0 5 2 5 1 23 50
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 1 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 6 6 3 10 10 17 2 – 2 10 0 2 0 1 0 0 1 1 1 1 5 14
Vac/Vac Membrane 3 4 3 0 14 6 5 10 9 – 1 3 3 20 23 11 7 7 2 8 34 16 96 139
Unique Cell Count 156 185 330 217 486 507 589 685 481 337 451 509 647 621 103 92 64 161 186 354 191 539 681
Labelled Cell Count 172 202 350 244 512 628 748 800 704 520 606 539 675 652 111 100 72 161 186 354 191 539 681


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.5 5.6 4.9 5.7 4.4 4.6 4.8 4.0 – 3.8 4.5 5.4 5.5 5.6 7.9 8.5 7.3 5.0 5.1 5.4
Std Deviation (1e-4) 0.9 0.9 1.2 1.2 1.3 1.1 1.0 1.0 0.8 – 1.1 0.9 1.2 1.3 1.4 1.8 2.1 2.3 1.0 1.1 1.2
Intensity Change (Log2) – – – -0.17 0.04 -0.34 -0.28 -0.21 -0.49 – -0.55 -0.29 -0.05 -0.02 0.01 0.5 0.61 0.4 -0.15 -0.14 -0.05

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP620RAP7000510WT3HU80HU120HU1600510WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30510WT1AF100AF140AF1800510
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.3 0.5 -0.3 0.5 0.2 -1.1 – 0.1 -0.5 0.7 2.2 4.5 2.6 1.9 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 2.7 0.6 0 0 0 0 – 0 0 0.6 -2.6 -2.1 0 0 0
Nucleus -0.5 -1.8 -3.6 -4.6 -2.8 -5.3 – -4.9 -3.7 -0.3 -2.4 -5.2 -4.9 -4.4 -3.6
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 1.9 0 0 0 0 – 0 0 0 2.1 2.5 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7536 2.7926 2.5919 2.3848 2.3842 2.5627 0.9918 1.1569 0.8252 0.4289 1.0686 0.8581 1.7205 1.6062 1.1737 1.6899 1.4616 1.175
Actin 0.0511 0.0002 0.0135 0.0007 0.1134 0.0157 0.0139 0 0.0118 0 0.0066 0.0002 0.0082 0 0.0004 0.0144 0.0033 0.0016
Bud 0.0003 0 0.0001 0.0001 0.0005 0.0001 0.0003 0 0.0002 0 0.0003 0 0.0003 0 0.0003 0.0002 0.0002 0.0001
Bud Neck 0.0016 0.0002 0.001 0.0001 0.0014 0.0011 0.0009 0.0001 0.0005 0 0.0017 0.0009 0.0017 0.0002 0.0002 0.0003 0.001 0.0007
Bud Periphery 0.0005 0 0.0003 0.0001 0.0006 0.0003 0.0004 0 0.0003 0 0.0008 0.0001 0.0007 0 0.0004 0.0002 0.0007 0.0002
Bud Site 0.0009 0.0002 0.0048 0.0001 0.0016 0.0002 0.0016 0.0001 0.0017 0 0.0008 0.0001 0.0005 0 0.0009 0.0005 0.0006 0.0002
Cell Periphery 0.0001 0 0.0047 0 0.0001 0 0.0003 0 0.0001 0 0.0002 0 0.0001 0 0.0001 0 0.0001 0
Cytoplasm 0.0097 0.0141 0.0017 0.0041 0.0012 0.0002 0.0023 0.0026 0.0006 0.0001 0.0018 0.0002 0.0021 0.0001 0.0027 0.0025 0.001 0.0003
Cytoplasmic Foci 0.0168 0.0007 0.0001 0.0006 0.0045 0.0004 0.0129 0.0001 0.0062 0 0.0072 0.0004 0.0102 0 0.0015 0.0372 0.0043 0.0022
Eisosomes 0.0005 0.0001 0.0011 0 0.0003 0.0001 0.0001 0 0 0 0.0001 0 0.0002 0 0 0.0002 0 0
Endoplasmic Reticulum 0.0049 0.0007 0.0043 0.0003 0.0003 0.0005 0.0008 0.0006 0.0004 0 0.0044 0.0003 0.0019 0 0.0004 0.0014 0.002 0.0004
Endosome 0.0169 0.0011 0.0006 0.0008 0.0035 0.0026 0.007 0.0006 0.0164 0 0.0321 0.0012 0.0084 0 0.0079 0.0103 0.0117 0.0028
Golgi 0.0112 0 0.0001 0.0001 0.0081 0.0016 0.0077 0 0.0072 0 0.0197 0.0004 0.0025 0 0.0014 0.003 0.0025 0.0019
Lipid Particles 0.0167 0.0002 0.0002 0.0001 0.0034 0.0021 0.0147 0.0001 0.0064 0 0.0121 0.0021 0.0082 0 0.004 0.0195 0.0084 0.0028
Mitochondria 0.0028 0.0002 0.0014 0.0047 0.0711 0.0099 0.0021 0.0001 0.0045 0.0001 0.0608 0.003 0.0094 0.0001 0.0037 0.0099 0.0036 0.005
None 0.0198 0.0357 0.0047 0.0022 0.0007 0.0001 0.0009 0.0005 0.0002 0 0.0015 0.0001 0.0043 0 0.0003 0.0021 0.001 0.0002
Nuclear Periphery 0.0226 0.0077 0.0091 0.0437 0.0062 0.0067 0.0065 0.0088 0.0024 0.0011 0.0315 0.002 0.0229 0.0022 0.0028 0.0211 0.0286 0.0069
Nucleolus 0.009 0.0101 0.0056 0.0045 0.0198 0.0211 0.0191 0.0057 0.0048 0.0034 0.0325 0.0246 0.0194 0.0075 0.0094 0.0043 0.0138 0.0176
Nucleus 0.7702 0.9134 0.9419 0.9291 0.7439 0.9333 0.8995 0.9768 0.927 0.9951 0.7595 0.9597 0.8666 0.9362 0.9244 0.7795 0.8113 0.9112
Peroxisomes 0.0301 0.0001 0.0002 0.0006 0.0142 0.0006 0.0021 0 0.0053 0 0.0072 0.0001 0.0081 0 0.0015 0.0285 0.0025 0.0013
Punctate Nuclear 0.0126 0.014 0.0026 0.0068 0.0044 0.0023 0.0015 0.002 0.0022 0.0003 0.012 0.0042 0.0213 0.0535 0.0352 0.064 0.0938 0.044
Vacuole 0.001 0.001 0.0016 0.0002 0.0004 0.0004 0.0045 0.0016 0.0015 0 0.0029 0.0002 0.0015 0 0.0017 0.0005 0.0043 0.0002
Vacuole Periphery 0.0009 0.0003 0.0004 0.0012 0.0003 0.0005 0.001 0.0002 0.0004 0 0.0043 0.0003 0.0016 0 0.0006 0.0005 0.005 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.912 7.264 7.9435 10.8737 10.5355 9.8311 10.0506 11.0883 10.078 12.6647
Translational Efficiency 0.9299 0.9021 0.92 0.6069 0.5311 0.5027 0.7036 0.5265 0.6264 0.5415

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2287 1737 159 1021 1017 1693 1763 1598 3304 3430 1922 2619

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 706.33 804.01 1137.51 929.23 756.52 807.27 975.80 863.47 721.78 805.62 989.18 889.11
Standard Deviation 77.58 118.54 147.78 144.59 96.49 168.89 127.63 131.51 86.99 145.60 136.87 140.47
Intensity Change Log 2 — 0.186871 0.687465 0.395693 — 0.093673 0.367207 0.190768 — 0.139425 0.530723 0.293352

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000062 0.000257 0.002850 0.001948 0.000149 0.000245 0.000629 0.000808 0.000089 0.000251 0.000813 0.001253
Bud Neck 0.001873 0.005120 0.011594 0.036760 0.001324 0.005328 0.014990 0.023050 0.001704 0.005223 0.014709 0.028395
Bud Site 0.001936 0.000543 0.002349 0.023647 0.000448 0.001781 0.001795 0.010601 0.001478 0.001154 0.001841 0.015687
Cell Periphery 0.000100 0.000064 0.000110 0.000276 0.000071 0.000095 0.000070 0.000194 0.000091 0.000079 0.000074 0.000226
Cytoplasm 0.017412 0.006770 0.003771 0.020722 0.019211 0.012268 0.003771 0.009033 0.017966 0.009484 0.003771 0.013590
Cytoplasmic Foci 0.001220 0.000770 0.000222 0.006391 0.001720 0.003459 0.000086 0.000206 0.001374 0.002097 0.000097 0.002617
Eisosomes 0.000008 0.000014 0.000087 0.000033 0.000015 0.000018 0.000021 0.000028 0.000010 0.000016 0.000027 0.000030
Endoplasmic Reticulum 0.001060 0.004098 0.015772 0.009119 0.002533 0.003691 0.006434 0.006692 0.001513 0.003897 0.007207 0.007638
Endosome 0.000371 0.000357 0.000604 0.011220 0.000524 0.001604 0.000297 0.000355 0.000418 0.000973 0.000323 0.004591
Golgi 0.000033 0.000063 0.000173 0.006998 0.000231 0.000955 0.000054 0.000078 0.000094 0.000503 0.000064 0.002776
Lipid Particles 0.000446 0.000374 0.000652 0.000448 0.000723 0.001796 0.000103 0.000139 0.000531 0.001076 0.000148 0.000259
Mitochondria 0.000699 0.001098 0.002036 0.008717 0.001304 0.002312 0.001706 0.002163 0.000885 0.001697 0.001733 0.004718
Mitotic Spindle 0.001144 0.001466 0.000585 0.047390 0.000443 0.004689 0.001635 0.003984 0.000928 0.003057 0.001549 0.020906
None 0.007393 0.003723 0.000994 0.007033 0.008623 0.004941 0.002033 0.008251 0.007772 0.004324 0.001947 0.007776
Nuclear Periphery 0.000746 0.001182 0.001343 0.003921 0.000766 0.001538 0.001241 0.001257 0.000752 0.001358 0.001249 0.002296
Nuclear Periphery Foci 0.000091 0.000571 0.000374 0.001607 0.000733 0.000709 0.000449 0.000168 0.000288 0.000639 0.000443 0.000729
Nucleolus 0.004462 0.010816 0.008285 0.003639 0.007842 0.007143 0.004688 0.005220 0.005503 0.009003 0.004986 0.004603
Nucleus 0.959936 0.961331 0.938188 0.782429 0.951673 0.943942 0.957154 0.923635 0.957393 0.952748 0.955585 0.868587
Peroxisomes 0.000281 0.000285 0.000859 0.000605 0.000519 0.000916 0.000115 0.000154 0.000354 0.000596 0.000176 0.000330
Vacuole 0.000628 0.000917 0.008900 0.018605 0.000867 0.001891 0.002552 0.003737 0.000702 0.001398 0.003077 0.009533
Vacuole Periphery 0.000101 0.000184 0.000254 0.008491 0.000282 0.000679 0.000176 0.000248 0.000156 0.000428 0.000183 0.003461

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.91 -13.84 -12.07 -10.61 5.53 -2.31 -14.02 -13.91 -11.15 -2.05 -7.39 -19.62 -17.81 -14.91 -3.46
Bud Neck -5.36 -10.05 -14.43 -12.79 -6.99 -5.20 -17.37 -14.94 -11.75 -4.54 -6.85 -17.68 -20.34 -17.25 -7.14
Bud Site 1.88 -1.89 -6.17 -7.23 -5.72 -2.24 -4.29 -6.02 -4.83 -4.76 0.50 -1.43 -7.85 -8.48 -7.49
Cell Periphery 2.29 -1.84 -3.62 -5.12 -2.69 -1.91 0.29 -4.40 -2.99 -4.74 0.81 1.63 -4.98 -5.74 -6.44
Cytoplasm 6.13 7.84 -0.29 -5.53 -7.16 2.66 6.85 4.37 2.17 -4.91 5.67 11.24 3.83 -2.13 -8.65
Cytoplasmic Foci 1.17 4.17 -1.93 -2.52 -3.70 -2.17 3.78 3.57 5.05 -1.64 -2.03 5.98 1.07 2.78 -4.03
Eisosomes -6.93 -12.62 -17.97 -14.05 7.51 -0.91 -5.62 -6.99 -6.47 -2.44 -6.06 -16.53 -19.61 -14.12 -1.95
Endoplasmic Reticulum -14.54 -12.63 -21.68 -14.88 3.61 -3.63 -12.58 -14.68 -9.72 -0.53 -13.42 -19.21 -27.80 -17.19 -2.97
Endosome -0.12 -1.97 -4.96 -4.99 -4.64 -2.27 1.42 0.87 2.78 -2.03 -2.27 0.50 -4.65 -2.90 -5.08
Golgi -3.27 -9.92 -3.14 -3.10 -2.93 -2.05 1.40 1.23 2.63 -2.35 -2.46 0.30 -3.04 -1.72 -3.09
Lipid Particles 0.19 -1.91 -1.02 -1.63 1.51 -2.05 3.87 3.65 3.38 -1.83 -2.19 3.74 2.27 3.19 -5.61
Mitochondria -1.98 -7.59 -5.38 -4.99 -3.68 -1.30 -0.95 -1.67 -0.35 -1.73 -2.60 -3.63 -6.35 -4.72 -4.16
Mitotic Spindle -0.50 0.12 -7.50 -7.40 -7.58 -3.22 -2.59 -4.30 -0.98 -2.98 -2.64 -1.63 -8.34 -6.94 -7.71
None 3.56 6.99 1.58 -1.93 -5.77 2.25 4.52 1.27 -1.24 -4.61 3.83 7.37 1.66 -2.21 -6.15
Nuclear Periphery -2.95 -3.75 -7.07 -6.29 -5.17 -2.88 -4.03 -6.83 -1.61 -2.12 -3.89 -4.57 -9.25 -6.27 -5.43
Nuclear Periphery Foci -2.53 -3.38 -6.34 -2.05 -3.25 0.61 1.82 1.82 1.55 -0.14 -1.94 -0.12 -2.80 -0.14 -2.20
Nucleolus -6.09 -3.70 1.67 7.34 4.53 0.98 2.90 1.42 0.62 -1.83 -4.70 0.32 0.54 5.27 0.24
Nucleus -0.04 6.63 16.15 16.19 10.97 1.14 -0.18 6.09 5.13 7.93 1.64 2.85 16.94 15.58 15.16
Peroxisomes -0.16 -3.69 -0.55 -0.90 5.27 -1.25 1.68 1.58 2.70 -1.06 -1.51 1.25 0.93 2.60 -1.41
Vacuole -1.27 -6.80 -12.03 -11.76 -5.00 -0.71 -2.85 -4.37 -4.07 -2.36 -1.88 -6.71 -12.77 -11.99 -8.61
Vacuole Periphery -2.71 -7.13 -4.20 -4.09 -3.95 -1.43 0.62 0.20 1.97 -1.44 -2.26 -0.42 -4.21 -3.26 -4.16
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Member of the NineTeen Complex (NTC); that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; relocalizes to the cytosol in response to hypoxia; homologs in human and C. elegans
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Syf1

Syf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Syf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available