Standard name
Human Ortholog
Description Member of the NineTeen Complex (NTC); that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; relocalizes to the cytosol in response to hypoxia; homologs in human and C. elegans
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.08 0 0.05 0.05 0.05 0 0.05 0.05 0 – 0.05 0 0.06 0.08 0.14 0.12 0.1 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.08 0.05 0.11 0.06 0.29 0.33 0.17 0.54 – 0.59 0.33 0.06 0 0 0 0 0.14 0 0 0 0 0 0
Nucleus 0.88 0.95 0.94 0.93 0.9 0.86 0.83 0.88 0.8 – 0.81 0.85 0.93 0.89 0.81 0.77 0.78 0.8 0.85 0.89 0.84 0.86 0.7 0.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.11 0.08 0.11 0 0.05 0.1 0.09 0.18 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 1 0 1
Bud 1 1 0 0 0 1 5 5 4 – 9 15 0 0 0 0 0 0 0 1 0 0 6 9
Bud Neck 0 0 1 1 1 1 1 4 1 – 2 3 0 0 0 0 0 0 0 0 0 1 3 9
Bud Site 0 0 0 1 1 6 6 5 20 – 9 16 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 13 4 15 11 26 21 31 33 15 – 16 17 29 54 87 12 9 4 4 0 2 0 1 2
Endoplasmic Reticulum 2 0 0 0 0 0 1 0 0 – 0 0 0 0 3 2 4 0 0 0 0 0 3 2
Endosome 0 0 1 0 0 0 1 0 0 – 0 1 0 2 6 0 4 1 3 0 2 0 2 8
Golgi 0 1 0 0 0 0 0 0 0 – 0 0 0 0 1 1 1 0 0 1 0 0 1 0
Mitochondria 13 15 15 23 27 147 192 118 258 – 200 151 28 11 13 4 2 9 1 1 3 0 7 19
Nucleus 138 175 309 201 438 434 489 603 386 – 273 384 474 575 504 79 72 51 136 166 297 163 379 408
Nuclear Periphery 0 0 0 0 2 1 4 4 8 – 1 2 1 4 5 1 1 0 1 0 1 0 4 4
Nucleolus 2 2 0 1 0 1 3 1 1 – 7 4 4 6 8 0 0 0 5 2 5 1 23 50
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 1 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 6 6 3 10 10 17 2 – 2 10 0 2 0 1 0 0 1 1 1 1 5 14
Vac/Vac Membrane 3 4 3 0 14 6 5 10 9 – 1 3 3 20 23 11 7 7 2 8 34 16 96 139
Unique Cell Count 156 185 330 217 486 507 589 685 481 337 451 509 647 621 103 92 64 161 186 354 191 539 681
Labelled Cell Count 172 202 350 244 512 628 748 800 704 520 606 539 675 652 111 100 72 161 186 354 191 539 681


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.5 5.6 4.9 5.7 4.4 4.6 4.8 4.0 – 3.8 4.5 5.4 5.5 5.6 7.9 8.5 7.3 5.0 5.1 5.4
Std Deviation (1e-4) 0.9 0.9 1.2 1.2 1.3 1.1 1.0 1.0 0.8 – 1.1 0.9 1.2 1.3 1.4 1.8 2.1 2.3 1.0 1.1 1.2
Intensity Change (Log2) – – – -0.17 0.04 -0.34 -0.28 -0.21 -0.49 – -0.55 -0.29 -0.05 -0.02 0.01 0.5 0.61 0.4 -0.15 -0.14 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.3 0.5 -0.3 0.5 0.2 -1.1 – 0.1 -0.5 0.7 2.2 4.5 2.6 1.9 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 2.7 0.6 0 0 0 0 – 0 0 0.6 -2.6 -2.1 0 0 0
Nucleus -0.5 -1.8 -3.6 -4.6 -2.8 -5.3 – -4.9 -3.7 -0.3 -2.4 -5.2 -4.9 -4.4 -3.6
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 1.9 0 0 0 0 – 0 0 0 2.1 2.5 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.7536 2.7926 2.5919 2.3848 2.3842 2.5627 0.9918 1.1569 0.8252 0.4289 1.0686 0.8581 1.7205 1.6062 1.1737 1.6899 1.4616 1.175
Actin 0.0511 0.0002 0.0135 0.0007 0.1134 0.0157 0.0139 0 0.0118 0 0.0066 0.0002 0.0082 0 0.0004 0.0144 0.0033 0.0016
Bud 0.0003 0 0.0001 0.0001 0.0005 0.0001 0.0003 0 0.0002 0 0.0003 0 0.0003 0 0.0003 0.0002 0.0002 0.0001
Bud Neck 0.0016 0.0002 0.001 0.0001 0.0014 0.0011 0.0009 0.0001 0.0005 0 0.0017 0.0009 0.0017 0.0002 0.0002 0.0003 0.001 0.0007
Bud Periphery 0.0005 0 0.0003 0.0001 0.0006 0.0003 0.0004 0 0.0003 0 0.0008 0.0001 0.0007 0 0.0004 0.0002 0.0007 0.0002
Bud Site 0.0009 0.0002 0.0048 0.0001 0.0016 0.0002 0.0016 0.0001 0.0017 0 0.0008 0.0001 0.0005 0 0.0009 0.0005 0.0006 0.0002
Cell Periphery 0.0001 0 0.0047 0 0.0001 0 0.0003 0 0.0001 0 0.0002 0 0.0001 0 0.0001 0 0.0001 0
Cytoplasm 0.0097 0.0141 0.0017 0.0041 0.0012 0.0002 0.0023 0.0026 0.0006 0.0001 0.0018 0.0002 0.0021 0.0001 0.0027 0.0025 0.001 0.0003
Cytoplasmic Foci 0.0168 0.0007 0.0001 0.0006 0.0045 0.0004 0.0129 0.0001 0.0062 0 0.0072 0.0004 0.0102 0 0.0015 0.0372 0.0043 0.0022
Eisosomes 0.0005 0.0001 0.0011 0 0.0003 0.0001 0.0001 0 0 0 0.0001 0 0.0002 0 0 0.0002 0 0
Endoplasmic Reticulum 0.0049 0.0007 0.0043 0.0003 0.0003 0.0005 0.0008 0.0006 0.0004 0 0.0044 0.0003 0.0019 0 0.0004 0.0014 0.002 0.0004
Endosome 0.0169 0.0011 0.0006 0.0008 0.0035 0.0026 0.007 0.0006 0.0164 0 0.0321 0.0012 0.0084 0 0.0079 0.0103 0.0117 0.0028
Golgi 0.0112 0 0.0001 0.0001 0.0081 0.0016 0.0077 0 0.0072 0 0.0197 0.0004 0.0025 0 0.0014 0.003 0.0025 0.0019
Lipid Particles 0.0167 0.0002 0.0002 0.0001 0.0034 0.0021 0.0147 0.0001 0.0064 0 0.0121 0.0021 0.0082 0 0.004 0.0195 0.0084 0.0028
Mitochondria 0.0028 0.0002 0.0014 0.0047 0.0711 0.0099 0.0021 0.0001 0.0045 0.0001 0.0608 0.003 0.0094 0.0001 0.0037 0.0099 0.0036 0.005
None 0.0198 0.0357 0.0047 0.0022 0.0007 0.0001 0.0009 0.0005 0.0002 0 0.0015 0.0001 0.0043 0 0.0003 0.0021 0.001 0.0002
Nuclear Periphery 0.0226 0.0077 0.0091 0.0437 0.0062 0.0067 0.0065 0.0088 0.0024 0.0011 0.0315 0.002 0.0229 0.0022 0.0028 0.0211 0.0286 0.0069
Nucleolus 0.009 0.0101 0.0056 0.0045 0.0198 0.0211 0.0191 0.0057 0.0048 0.0034 0.0325 0.0246 0.0194 0.0075 0.0094 0.0043 0.0138 0.0176
Nucleus 0.7702 0.9134 0.9419 0.9291 0.7439 0.9333 0.8995 0.9768 0.927 0.9951 0.7595 0.9597 0.8666 0.9362 0.9244 0.7795 0.8113 0.9112
Peroxisomes 0.0301 0.0001 0.0002 0.0006 0.0142 0.0006 0.0021 0 0.0053 0 0.0072 0.0001 0.0081 0 0.0015 0.0285 0.0025 0.0013
Punctate Nuclear 0.0126 0.014 0.0026 0.0068 0.0044 0.0023 0.0015 0.002 0.0022 0.0003 0.012 0.0042 0.0213 0.0535 0.0352 0.064 0.0938 0.044
Vacuole 0.001 0.001 0.0016 0.0002 0.0004 0.0004 0.0045 0.0016 0.0015 0 0.0029 0.0002 0.0015 0 0.0017 0.0005 0.0043 0.0002
Vacuole Periphery 0.0009 0.0003 0.0004 0.0012 0.0003 0.0005 0.001 0.0002 0.0004 0 0.0043 0.0003 0.0016 0 0.0006 0.0005 0.005 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.912 7.264 7.9435 10.8737 10.5355 9.8311 10.0506 11.0883 10.078 12.6647
Translational Efficiency 0.9299 0.9021 0.92 0.6069 0.5311 0.5027 0.7036 0.5265 0.6264 0.5415

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Member of the NineTeen Complex (NTC); that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; relocalizes to the cytosol in response to hypoxia; homologs in human and C. elegans
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Syf1

Syf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Syf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available