Standard name | |
---|---|
Human Ortholog | |
Description | Member of the NineTeen Complex (NTC); that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; relocalizes to the cytosol in response to hypoxia; homologs in human and C. elegans |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 |
Bud | 1 | 1 | 0 | 0 | 0 | 1 | 5 | 5 | 4 | – | 9 | 15 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 6 | 9 |
Bud Neck | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 4 | 1 | – | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 9 |
Bud Site | 0 | 0 | 0 | 1 | 1 | 6 | 6 | 5 | 20 | – | 9 | 16 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 13 | 4 | 15 | 11 | 26 | 21 | 31 | 33 | 15 | – | 16 | 17 | 29 | 54 | 87 | 12 | 9 | 4 | 4 | 0 | 2 | 0 | 1 | 2 |
Endoplasmic Reticulum | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 3 | 2 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 0 | 2 | 6 | 0 | 4 | 1 | 3 | 0 | 2 | 0 | 2 | 8 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 |
Mitochondria | 13 | 15 | 15 | 23 | 27 | 147 | 192 | 118 | 258 | – | 200 | 151 | 28 | 11 | 13 | 4 | 2 | 9 | 1 | 1 | 3 | 0 | 7 | 19 |
Nucleus | 138 | 175 | 309 | 201 | 438 | 434 | 489 | 603 | 386 | – | 273 | 384 | 474 | 575 | 504 | 79 | 72 | 51 | 136 | 166 | 297 | 163 | 379 | 408 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 2 | 1 | 4 | 4 | 8 | – | 1 | 2 | 1 | 4 | 5 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 4 | 4 |
Nucleolus | 2 | 2 | 0 | 1 | 0 | 1 | 3 | 1 | 1 | – | 7 | 4 | 4 | 6 | 8 | 0 | 0 | 0 | 5 | 2 | 5 | 1 | 23 | 50 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 6 | 6 | 3 | 10 | 10 | 17 | 2 | – | 2 | 10 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 5 | 14 |
Vac/Vac Membrane | 3 | 4 | 3 | 0 | 14 | 6 | 5 | 10 | 9 | – | 1 | 3 | 3 | 20 | 23 | 11 | 7 | 7 | 2 | 8 | 34 | 16 | 96 | 139 |
Unique Cell Count | 156 | 185 | 330 | 217 | 486 | 507 | 589 | 685 | 481 | 337 | 451 | 509 | 647 | 621 | 103 | 92 | 64 | 161 | 186 | 354 | 191 | 539 | 681 | |
Labelled Cell Count | 172 | 202 | 350 | 244 | 512 | 628 | 748 | 800 | 704 | 520 | 606 | 539 | 675 | 652 | 111 | 100 | 72 | 161 | 186 | 354 | 191 | 539 | 681 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.4 | 5.5 | 5.6 | 4.9 | 5.7 | 4.4 | 4.6 | 4.8 | 4.0 | – | 3.8 | 4.5 | 5.4 | 5.5 | 5.6 | 7.9 | 8.5 | 7.3 | 5.0 | 5.1 | 5.4 |
Std Deviation (1e-4) | 0.9 | 0.9 | 1.2 | 1.2 | 1.3 | 1.1 | 1.0 | 1.0 | 0.8 | – | 1.1 | 0.9 | 1.2 | 1.3 | 1.4 | 1.8 | 2.1 | 2.3 | 1.0 | 1.1 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.17 | 0.04 | -0.34 | -0.28 | -0.21 | -0.49 | – | -0.55 | -0.29 | -0.05 | -0.02 | 0.01 | 0.5 | 0.61 | 0.4 | -0.15 | -0.14 | -0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.3 | 0.5 | -0.3 | 0.5 | 0.2 | -1.1 | – | 0.1 | -0.5 | 0.7 | 2.2 | 4.5 | 2.6 | 1.9 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.7 | 0.6 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0.6 | -2.6 | -2.1 | 0 | 0 | 0 |
Nucleus | -0.5 | -1.8 | -3.6 | -4.6 | -2.8 | -5.3 | – | -4.9 | -3.7 | -0.3 | -2.4 | -5.2 | -4.9 | -4.4 | -3.6 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 1.9 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2.1 | 2.5 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.7536 | 2.7926 | 2.5919 | 2.3848 | 2.3842 | 2.5627 | 0.9918 | 1.1569 | 0.8252 | 0.4289 | 1.0686 | 0.8581 | 1.7205 | 1.6062 | 1.1737 | 1.6899 | 1.4616 | 1.175 |
Actin | 0.0511 | 0.0002 | 0.0135 | 0.0007 | 0.1134 | 0.0157 | 0.0139 | 0 | 0.0118 | 0 | 0.0066 | 0.0002 | 0.0082 | 0 | 0.0004 | 0.0144 | 0.0033 | 0.0016 |
Bud | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0.0003 | 0 | 0.0003 | 0 | 0.0003 | 0.0002 | 0.0002 | 0.0001 |
Bud Neck | 0.0016 | 0.0002 | 0.001 | 0.0001 | 0.0014 | 0.0011 | 0.0009 | 0.0001 | 0.0005 | 0 | 0.0017 | 0.0009 | 0.0017 | 0.0002 | 0.0002 | 0.0003 | 0.001 | 0.0007 |
Bud Periphery | 0.0005 | 0 | 0.0003 | 0.0001 | 0.0006 | 0.0003 | 0.0004 | 0 | 0.0003 | 0 | 0.0008 | 0.0001 | 0.0007 | 0 | 0.0004 | 0.0002 | 0.0007 | 0.0002 |
Bud Site | 0.0009 | 0.0002 | 0.0048 | 0.0001 | 0.0016 | 0.0002 | 0.0016 | 0.0001 | 0.0017 | 0 | 0.0008 | 0.0001 | 0.0005 | 0 | 0.0009 | 0.0005 | 0.0006 | 0.0002 |
Cell Periphery | 0.0001 | 0 | 0.0047 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.0097 | 0.0141 | 0.0017 | 0.0041 | 0.0012 | 0.0002 | 0.0023 | 0.0026 | 0.0006 | 0.0001 | 0.0018 | 0.0002 | 0.0021 | 0.0001 | 0.0027 | 0.0025 | 0.001 | 0.0003 |
Cytoplasmic Foci | 0.0168 | 0.0007 | 0.0001 | 0.0006 | 0.0045 | 0.0004 | 0.0129 | 0.0001 | 0.0062 | 0 | 0.0072 | 0.0004 | 0.0102 | 0 | 0.0015 | 0.0372 | 0.0043 | 0.0022 |
Eisosomes | 0.0005 | 0.0001 | 0.0011 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0 |
Endoplasmic Reticulum | 0.0049 | 0.0007 | 0.0043 | 0.0003 | 0.0003 | 0.0005 | 0.0008 | 0.0006 | 0.0004 | 0 | 0.0044 | 0.0003 | 0.0019 | 0 | 0.0004 | 0.0014 | 0.002 | 0.0004 |
Endosome | 0.0169 | 0.0011 | 0.0006 | 0.0008 | 0.0035 | 0.0026 | 0.007 | 0.0006 | 0.0164 | 0 | 0.0321 | 0.0012 | 0.0084 | 0 | 0.0079 | 0.0103 | 0.0117 | 0.0028 |
Golgi | 0.0112 | 0 | 0.0001 | 0.0001 | 0.0081 | 0.0016 | 0.0077 | 0 | 0.0072 | 0 | 0.0197 | 0.0004 | 0.0025 | 0 | 0.0014 | 0.003 | 0.0025 | 0.0019 |
Lipid Particles | 0.0167 | 0.0002 | 0.0002 | 0.0001 | 0.0034 | 0.0021 | 0.0147 | 0.0001 | 0.0064 | 0 | 0.0121 | 0.0021 | 0.0082 | 0 | 0.004 | 0.0195 | 0.0084 | 0.0028 |
Mitochondria | 0.0028 | 0.0002 | 0.0014 | 0.0047 | 0.0711 | 0.0099 | 0.0021 | 0.0001 | 0.0045 | 0.0001 | 0.0608 | 0.003 | 0.0094 | 0.0001 | 0.0037 | 0.0099 | 0.0036 | 0.005 |
None | 0.0198 | 0.0357 | 0.0047 | 0.0022 | 0.0007 | 0.0001 | 0.0009 | 0.0005 | 0.0002 | 0 | 0.0015 | 0.0001 | 0.0043 | 0 | 0.0003 | 0.0021 | 0.001 | 0.0002 |
Nuclear Periphery | 0.0226 | 0.0077 | 0.0091 | 0.0437 | 0.0062 | 0.0067 | 0.0065 | 0.0088 | 0.0024 | 0.0011 | 0.0315 | 0.002 | 0.0229 | 0.0022 | 0.0028 | 0.0211 | 0.0286 | 0.0069 |
Nucleolus | 0.009 | 0.0101 | 0.0056 | 0.0045 | 0.0198 | 0.0211 | 0.0191 | 0.0057 | 0.0048 | 0.0034 | 0.0325 | 0.0246 | 0.0194 | 0.0075 | 0.0094 | 0.0043 | 0.0138 | 0.0176 |
Nucleus | 0.7702 | 0.9134 | 0.9419 | 0.9291 | 0.7439 | 0.9333 | 0.8995 | 0.9768 | 0.927 | 0.9951 | 0.7595 | 0.9597 | 0.8666 | 0.9362 | 0.9244 | 0.7795 | 0.8113 | 0.9112 |
Peroxisomes | 0.0301 | 0.0001 | 0.0002 | 0.0006 | 0.0142 | 0.0006 | 0.0021 | 0 | 0.0053 | 0 | 0.0072 | 0.0001 | 0.0081 | 0 | 0.0015 | 0.0285 | 0.0025 | 0.0013 |
Punctate Nuclear | 0.0126 | 0.014 | 0.0026 | 0.0068 | 0.0044 | 0.0023 | 0.0015 | 0.002 | 0.0022 | 0.0003 | 0.012 | 0.0042 | 0.0213 | 0.0535 | 0.0352 | 0.064 | 0.0938 | 0.044 |
Vacuole | 0.001 | 0.001 | 0.0016 | 0.0002 | 0.0004 | 0.0004 | 0.0045 | 0.0016 | 0.0015 | 0 | 0.0029 | 0.0002 | 0.0015 | 0 | 0.0017 | 0.0005 | 0.0043 | 0.0002 |
Vacuole Periphery | 0.0009 | 0.0003 | 0.0004 | 0.0012 | 0.0003 | 0.0005 | 0.001 | 0.0002 | 0.0004 | 0 | 0.0043 | 0.0003 | 0.0016 | 0 | 0.0006 | 0.0005 | 0.005 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.912 | 7.264 | 7.9435 | 10.8737 | 10.5355 | 9.8311 | 10.0506 | 11.0883 | 10.078 | 12.6647 |
Translational Efficiency | 0.9299 | 0.9021 | 0.92 | 0.6069 | 0.5311 | 0.5027 | 0.7036 | 0.5265 | 0.6264 | 0.5415 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|