Standard name | |
---|---|
Human Ortholog | |
Description | Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 3 | 2 | 4 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 1 | 0 | – | 1 | 3 | 10 | 6 | 1 | 1 | 0 | 3 | 4 | 2 | 9 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 198 | 292 | 121 | 58 | 92 | 65 | – | 85 | 73 | 60 | 50 | 30 | 231 | 226 | 254 | 337 | 334 | 399 | 180 | 278 | 117 | 181 | 280 | 299 |
Endoplasmic Reticulum | 0 | 3 | 0 | 0 | 1 | 0 | – | 12 | 8 | 15 | 6 | 7 | 5 | 2 | 5 | 18 | 22 | 23 | 0 | 5 | 6 | 5 | 13 | 9 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 5 | 2 | 2 | 3 | 5 | 0 | 0 | 1 | 0 | 1 | 3 | 0 | 0 | 0 | 0 | 1 | 1 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 5 | 4 | 10 | 8 | 0 | 0 | 0 | 0 | 5 | 12 | 0 | 0 | 0 | 1 | 1 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 9 | 1 | 5 | 0 | 5 | 5 | 5 |
Unique Cell Count | 198 | 296 | 122 | 58 | 93 | 66 | 105 | 88 | 85 | 69 | 47 | 233 | 229 | 260 | 344 | 346 | 425 | 191 | 301 | 132 | 203 | 317 | 331 | |
Labelled Cell Count | 198 | 296 | 122 | 58 | 94 | 66 | 109 | 94 | 93 | 80 | 53 | 237 | 229 | 264 | 360 | 368 | 457 | 191 | 301 | 132 | 203 | 317 | 331 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 221.9 | 253.4 | 118.6 | 134.0 | 114.5 | 103.4 | – | 83.8 | 71.4 | 72.6 | 68.6 | 62.9 | 191.3 | 180.5 | 170.8 | 228.5 | 219.3 | 216.0 | 190.5 | 195.6 | 204.6 |
Std Deviation (1e-4) | 52.3 | 64.5 | 39.3 | 38.0 | 38.6 | 37.9 | – | 27.7 | 22.7 | 22.2 | 23.8 | 20.3 | 54.9 | 62.3 | 54.2 | 77.4 | 71.5 | 76.9 | 67.5 | 70.6 | 73.4 |
Intensity Change (Log2) | – | – | – | 0.18 | -0.05 | -0.2 | – | -0.5 | -0.73 | -0.71 | -0.79 | -0.92 | 0.69 | 0.61 | 0.53 | 0.95 | 0.89 | 0.86 | 0.68 | 0.72 | 0.79 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.7 | -0.2 | -0.4 | – | -4.7 | -4.4 | -6.1 | -5.8 | -6.7 | 0 | -0.4 | -1.0 | -0.9 | -1.5 | -2.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 3.8 | 0 | 4.8 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 2.6 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 227.9761 | 294.6392 | 259.552 | 290.7605 | 240.0931 | 263.6969 | 200.4166 | 270.073 | 231.7443 | 262.815 | 226.7108 | 216.0426 | 171.8682 | 315.3212 | 269.5141 | 225.5476 | 153.1848 | 244.6882 |
Actin | 0.0127 | 0.0012 | 0.0119 | 0.0008 | 0.0006 | 0.0343 | 0.0135 | 0.0008 | 0.0107 | 0.0008 | 0.0017 | 0.0054 | 0.0171 | 0.0012 | 0.0055 | 0.0013 | 0.0111 | 0.0065 |
Bud | 0.007 | 0.0039 | 0.0079 | 0.0027 | 0.0033 | 0.0086 | 0.0055 | 0.0037 | 0.0051 | 0.0034 | 0.0064 | 0.0163 | 0.0056 | 0.0032 | 0.0084 | 0.0035 | 0.0115 | 0.0191 |
Bud Neck | 0.0013 | 0.0017 | 0.0018 | 0.0013 | 0.0022 | 0.006 | 0.0015 | 0.0009 | 0.0018 | 0.001 | 0.0012 | 0.0026 | 0.0011 | 0.0007 | 0.0018 | 0.0015 | 0.0026 | 0.0031 |
Bud Periphery | 0.0081 | 0.0024 | 0.0068 | 0.002 | 0.0015 | 0.0075 | 0.0078 | 0.0026 | 0.0045 | 0.0018 | 0.0057 | 0.0347 | 0.0071 | 0.0019 | 0.008 | 0.0028 | 0.0278 | 0.0185 |
Bud Site | 0.0036 | 0.003 | 0.0048 | 0.0008 | 0.0004 | 0.0019 | 0.0031 | 0.002 | 0.0026 | 0.0005 | 0.0007 | 0.0015 | 0.0035 | 0.0031 | 0.0035 | 0.0021 | 0.0014 | 0.0038 |
Cell Periphery | 0.0774 | 0.0617 | 0.0535 | 0.1009 | 0.0339 | 0.035 | 0.0865 | 0.0505 | 0.0465 | 0.0641 | 0.0169 | 0.0163 | 0.0663 | 0.0271 | 0.0537 | 0.0506 | 0.0155 | 0.0552 |
Cytoplasm | 0.3839 | 0.6335 | 0.5427 | 0.2026 | 0.5403 | 0.5366 | 0.398 | 0.7288 | 0.5023 | 0.3425 | 0.5455 | 0.3589 | 0.3655 | 0.7797 | 0.6353 | 0.3267 | 0.4126 | 0.5896 |
Cytoplasmic Foci | 0.0068 | 0.0013 | 0.0109 | 0.001 | 0.0039 | 0.0106 | 0.0084 | 0.0013 | 0.0062 | 0.0015 | 0.0022 | 0.0072 | 0.0115 | 0.0019 | 0.0046 | 0.0135 | 0.02 | 0.0023 |
Eisosomes | 0.0023 | 0.0005 | 0.0008 | 0.0001 | 0.0015 | 0.002 | 0.0019 | 0 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0036 | 0.0004 | 0.0006 | 0.0011 | 0.0007 | 0.0004 |
Endoplasmic Reticulum | 0.0394 | 0.0136 | 0.0245 | 0.0014 | 0.018 | 0.0241 | 0.0364 | 0.0066 | 0.0101 | 0.0017 | 0.002 | 0.0149 | 0.0355 | 0.0068 | 0.0055 | 0.0103 | 0.0044 | 0.0053 |
Endosome | 0.0086 | 0.0035 | 0.0077 | 0.0036 | 0.0133 | 0.0273 | 0.008 | 0.0022 | 0.0154 | 0.0062 | 0.0099 | 0.0375 | 0.0243 | 0.0031 | 0.0074 | 0.0175 | 0.0131 | 0.0065 |
Golgi | 0.0025 | 0.0005 | 0.004 | 0.0003 | 0.0009 | 0.024 | 0.0068 | 0.0004 | 0.0097 | 0.0008 | 0.0005 | 0.0067 | 0.0043 | 0.0005 | 0.0022 | 0.0022 | 0.0047 | 0.0022 |
Lipid Particles | 0.0034 | 0.0012 | 0.0025 | 0.0035 | 0.0107 | 0.0065 | 0.0035 | 0.0013 | 0.0093 | 0.0015 | 0.0012 | 0.0025 | 0.0091 | 0.0014 | 0.0034 | 0.0193 | 0.0037 | 0.0066 |
Mitochondria | 0.0606 | 0.0113 | 0.0213 | 0.0028 | 0.0039 | 0.0459 | 0.0366 | 0.0034 | 0.0132 | 0.0029 | 0.0162 | 0.0477 | 0.0588 | 0.0124 | 0.0144 | 0.0144 | 0.0804 | 0.0833 |
None | 0.0106 | 0.0009 | 0.0071 | 0.0007 | 0.0094 | 0.0056 | 0.0055 | 0.0007 | 0.0029 | 0.0009 | 0.0008 | 0.0022 | 0.0181 | 0.002 | 0.0043 | 0.023 | 0.0423 | 0.0028 |
Nuclear Periphery | 0.0573 | 0.0148 | 0.014 | 0.0279 | 0.0145 | 0.0301 | 0.0651 | 0.0052 | 0.0125 | 0.0082 | 0.0223 | 0.0246 | 0.1048 | 0.01 | 0.0156 | 0.0301 | 0.0261 | 0.0357 |
Nucleolus | 0.0087 | 0.0029 | 0.0072 | 0.015 | 0.0031 | 0.0073 | 0.0087 | 0.0015 | 0.0122 | 0.0053 | 0.0026 | 0.0066 | 0.021 | 0.0071 | 0.008 | 0.0363 | 0.0089 | 0.004 |
Nucleus | 0.0324 | 0.0178 | 0.0169 | 0.0269 | 0.0146 | 0.0146 | 0.0348 | 0.006 | 0.0122 | 0.0166 | 0.0144 | 0.0586 | 0.0343 | 0.0117 | 0.0183 | 0.0385 | 0.0379 | 0.0311 |
Peroxisomes | 0.0006 | 0.0003 | 0.0008 | 0.0003 | 0.0003 | 0.0021 | 0.0006 | 0.0002 | 0.0009 | 0.0002 | 0.0002 | 0.0013 | 0.0012 | 0.0003 | 0.0006 | 0.001 | 0.0016 | 0.0006 |
Punctate Nuclear | 0.0004 | 0.0001 | 0.0009 | 0.0001 | 0.0002 | 0.0004 | 0.0006 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0006 | 0.0028 | 0.0001 | 0.0003 | 0.0021 | 0.0006 | 0.0003 |
Vacuole | 0.1924 | 0.1854 | 0.1862 | 0.3624 | 0.2231 | 0.0972 | 0.207 | 0.16 | 0.2573 | 0.4147 | 0.2255 | 0.2125 | 0.1383 | 0.104 | 0.1588 | 0.2844 | 0.1924 | 0.0827 |
Vacuole Periphery | 0.08 | 0.0386 | 0.0657 | 0.2429 | 0.1003 | 0.0724 | 0.0602 | 0.0219 | 0.0637 | 0.1253 | 0.1238 | 0.1414 | 0.0662 | 0.0215 | 0.0397 | 0.1177 | 0.0807 | 0.0406 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 2512.5716 | 2606.5684 | 3105.574 | 2528.541 | 2723.3339 | 2347.3117 | 2363.6377 | 2146.4353 | 2333.624 | 2204.555 |
Translational Efficiency | 1.4016 | 1.3947 | 1.2056 | 1.4002 | 1.5806 | 1.5314 | 1.3839 | 1.4737 | 1.4159 | 1.481 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1907 | 2034 | 495 | 184 | 2339 | 164 | 2305 | 1388 | 4246 | 2198 | 2800 | 1572 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 7476.17 | 9864.26 | 16305.94 | 11849.29 | 8919.16 | 14003.15 | 13318.40 | 10046.52 | 8271.07 | 10173.08 | 13846.55 | 10257.53 |
Standard Deviation | 1942.42 | 3256.80 | 3724.08 | 6024.90 | 2595.43 | 2898.78 | 2950.86 | 3418.89 | 2433.22 | 3409.56 | 3304.38 | 3860.74 |
Intensity Change Log 2 | — | 0.399911 | 1.125026 | 0.664429 | — | 0.650772 | 0.578441 | 0.171716 | — | 0.541757 | 0.853498 | 0.417370 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000056 | 0.000111 | 0.000144 | 0.000448 | 0.000036 | 0.000051 | 0.000084 | 0.000440 | 0.000045 | 0.000107 | 0.000095 | 0.000441 |
Bud Neck | 0.000219 | 0.000406 | 0.002591 | 0.002265 | 0.000153 | 0.000607 | 0.000469 | 0.001914 | 0.000182 | 0.000421 | 0.000844 | 0.001955 |
Bud Site | 0.002932 | 0.005098 | 0.003317 | 0.013667 | 0.001446 | 0.001350 | 0.002859 | 0.015470 | 0.002113 | 0.004818 | 0.002940 | 0.015259 |
Cell Periphery | 0.027013 | 0.021842 | 0.084533 | 0.071185 | 0.032891 | 0.081790 | 0.036954 | 0.058713 | 0.030251 | 0.026315 | 0.045365 | 0.060173 |
Cytoplasm | 0.481577* | 0.522421* | 0.066400 | 0.272355* | 0.530348* | 0.108720* | 0.405827* | 0.491887* | 0.508444* | 0.491553* | 0.345821* | 0.466191* |
Cytoplasmic Foci | 0.012972 | 0.014924 | 0.008270 | 0.011299 | 0.010454 | 0.010520 | 0.008237 | 0.005776 | 0.011585 | 0.014596 | 0.008243 | 0.006423 |
Eisosomes | 0.000002 | 0.000003 | 0.000016 | 0.000010 | 0.000002 | 0.000007 | 0.000003 | 0.000008 | 0.000002 | 0.000004 | 0.000005 | 0.000008 |
Endoplasmic Reticulum | 0.005551 | 0.004063 | 0.001128 | 0.000599 | 0.005491 | 0.000556 | 0.004696 | 0.001222 | 0.005518 | 0.003801 | 0.004065 | 0.001149 |
Endosome | 0.001120 | 0.001565 | 0.000813 | 0.001590 | 0.000958 | 0.000732 | 0.001335 | 0.001294 | 0.001031 | 0.001502 | 0.001243 | 0.001329 |
Golgi | 0.099192 | 0.072459 | 0.086210 | 0.029568 | 0.058183 | 0.162805* | 0.051954 | 0.019380 | 0.076601 | 0.079200 | 0.058010 | 0.020573 |
Lipid Particles | 0.021886 | 0.021624 | 0.034126 | 0.030545 | 0.023884 | 0.044507 | 0.027651 | 0.028127 | 0.022986 | 0.023331 | 0.028795 | 0.028410 |
Mitochondria | 0.000472 | 0.000662 | 0.000173 | 0.000687 | 0.000337 | 0.000073 | 0.000419 | 0.000553 | 0.000398 | 0.000618 | 0.000376 | 0.000569 |
Mitotic Spindle | 0.000708 | 0.000934 | 0.001286 | 0.003650 | 0.000577 | 0.000979 | 0.000699 | 0.000737 | 0.000636 | 0.000937 | 0.000803 | 0.001078 |
None | 0.001857 | 0.003081 | 0.000677 | 0.001445 | 0.001663 | 0.000970 | 0.001308 | 0.002950 | 0.001750 | 0.002924 | 0.001196 | 0.002774 |
Nuclear Periphery | 0.000930 | 0.000795 | 0.004003 | 0.000762 | 0.001240 | 0.002186 | 0.001227 | 0.000592 | 0.001101 | 0.000899 | 0.001718 | 0.000612 |
Nuclear Periphery Foci | 0.013340 | 0.013453 | 0.012148 | 0.005730 | 0.012694 | 0.015805 | 0.014700 | 0.007374 | 0.012984 | 0.013628 | 0.014248 | 0.007181 |
Nucleolus | 0.000113 | 0.000112 | 0.000132 | 0.000244 | 0.000084 | 0.000089 | 0.000114 | 0.000278 | 0.000097 | 0.000110 | 0.000117 | 0.000274 |
Nucleus | 0.002683 | 0.004214 | 0.003672 | 0.011839 | 0.002630 | 0.002009 | 0.003918 | 0.010008 | 0.002654 | 0.004049 | 0.003874 | 0.010222 |
Peroxisomes | 0.000045 | 0.000076 | 0.000019 | 0.000135 | 0.000079 | 0.000011 | 0.000104 | 0.000211 | 0.000064 | 0.000071 | 0.000089 | 0.000202 |
Vacuole | 0.136476 | 0.145802 | 0.443476* | 0.454814* | 0.144135 | 0.299825* | 0.285543* | 0.304386* | 0.140695 | 0.157294 | 0.313463* | 0.321994* |
Vacuole Periphery | 0.190857* | 0.166357* | 0.246867* | 0.087162 | 0.172715* | 0.266407* | 0.151899* | 0.048679 | 0.180863* | 0.173822* | 0.168688* | 0.053183 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.14 | -3.41 | -1.86 | -1.63 | -1.40 | -1.69 | -3.31 | -4.19 | -4.03 | -3.59 | -2.51 | -4.13 | -4.52 | -3.63 | -3.79 |
Bud Neck | -4.13 | -7.21 | -5.59 | -5.14 | 0.92 | -4.46 | -6.71 | -10.21 | -7.35 | -7.86 | -6.08 | -9.35 | -11.23 | -9.79 | -6.03 |
Bud Site | -2.84 | -0.92 | -3.67 | -3.02 | -3.39 | 0.44 | -5.50 | -11.53 | -11.51 | -10.19 | -5.39 | -2.60 | -11.41 | -8.83 | -10.69 |
Cell Periphery | 3.26 | -13.80 | -5.62 | -6.46 | 2.67 | -6.37 | -1.72 | -7.95 | 3.27 | -6.55 | 2.71 | -8.44 | -10.34 | -11.43 | -4.33 |
Cytoplasm | -3.43 | 38.72 | 7.68 | 9.26 | -8.28 | 24.14 | 12.27 | 3.20 | -20.66 | -7.35 | 1.82 | 19.10 | 3.91 | 2.03 | -10.95 |
Cytoplasmic Foci | -1.59 | 2.13 | -0.19 | 0.29 | -1.02 | -0.20 | 2.79 | 4.14 | 2.28 | 2.08 | -3.06 | 4.57 | 4.56 | 6.29 | 1.25 |
Eisosomes | -1.19 | -7.45 | -3.73 | -3.17 | 0.38 | -3.44 | -4.76 | -7.80 | -0.95 | -6.17 | -1.75 | -8.26 | -8.40 | -3.75 | -3.87 |
Endoplasmic Reticulum | 2.28 | 7.86 | 8.38 | 8.12 | 1.57 | 9.09 | 1.48 | 7.99 | -4.49 | 10.67 | 3.37 | 3.38 | 11.23 | 7.63 | 10.61 |
Endosome | -4.32 | 3.10 | -0.73 | 0.01 | -1.21 | 1.87 | -4.26 | -1.53 | -2.34 | 0.22 | -5.36 | -2.90 | -1.43 | 0.93 | -0.36 |
Golgi | 7.36 | 3.14 | 15.76 | 10.87 | 10.36 | -9.16 | 3.05 | 19.55 | 12.76 | 17.52* | -0.82 | 8.96 | 29.35 | 23.65 | 20.76* |
Lipid Particles | 0.12 | -5.76 | -1.75 | -1.78 | 1.71 | -3.63 | -2.15 | -1.86 | 2.91 | -0.14 | -0.28 | -4.44 | -2.75 | -2.20 | 0.53 |
Mitochondria | -1.48 | 2.65 | -0.88 | -0.58 | -1.26 | 7.32 | -1.36 | -2.51 | -5.13 | -0.87 | -2.20 | -0.38 | -2.05 | -0.19 | -1.56 |
Mitotic Spindle | -2.99 | -3.74 | -1.14 | -1.04 | -0.80 | -3.23 | -3.43 | -1.43 | 0.61 | -0.73 | -4.41 | -4.39 | -1.59 | -0.60 | -0.92 |
None | -2.44 | 9.66 | 0.14 | 1.62 | -1.62 | 4.00 | 1.27 | -1.80 | -2.75 | -2.18 | -2.54 | 2.50 | -1.68 | 0.14 | -2.42 |
Nuclear Periphery | 2.25 | -11.30 | 0.31 | -0.76 | 10.79 | -4.13 | -0.13 | 3.95 | 8.53 | 7.82 | 2.12 | -6.16 | 4.58 | 3.96 | 12.53 |
Nuclear Periphery Foci | -0.19 | 0.84 | 10.32 | 10.42 | 7.67 | -1.74 | -2.75 | 5.71 | 4.55 | 7.79 | -0.98 | -2.36 | 7.44 | 7.43 | 8.66 |
Nucleolus | -0.05 | -0.95 | -3.14 | -3.89 | -3.14 | -0.38 | -3.26 | -6.35 | -5.66 | -4.90 | -0.97 | -1.69 | -5.91 | -5.96 | -5.34 |
Nucleus | -2.99 | -3.07 | -3.59 | -3.25 | -3.18 | 1.15 | -3.36 | -9.83 | -10.08 | -7.45 | -3.02 | -4.21 | -10.16 | -7.88 | -7.97 |
Peroxisomes | -2.87 | 4.38 | -1.94 | -1.20 | -2.56 | 6.12 | -1.21 | -3.66 | -6.16 | -2.93 | -0.63 | -1.74 | -4.49 | -4.16 | -3.52 |
Vacuole | -1.77 | -27.24 | -13.64 | -13.19 | -0.22 | -8.15 | -22.87 | -19.40 | -0.28 | -1.61 | -3.46 | -32.03 | -23.68 | -19.84 | -0.48 |
Vacuole Periphery | 4.10 | -5.09 | 11.32 | 9.09 | 13.36 | -5.74 | 4.64 | 28.98* | 13.90 | 27.54* | 1.58 | 3.65 | 36.08 | 27.76 | 31.33* |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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