Standard name | |
---|---|
Human Ortholog | |
Description | Lysine-specific metalloprotease of the pitrilysin family; metalloprotease of the intermembrane space; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 0 | 2 | 3 | 4 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 0 | 2 | 0 | 3 | 4 | 1 | 5 | 10 | 13 |
Bud | 0 | 4 | 17 | 18 | 15 | 14 | 15 | 14 | 9 | 13 | 16 | 27 | 3 | 4 | 8 | 10 | 0 | 5 | 0 | 1 | 0 | 1 | 2 | 1 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 1 | 4 | 1 | 1 | 3 | 4 | 2 | 5 | 5 | 5 | 4 | 0 | 0 | 1 | 3 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 5 | 10 | 3 | 4 | 5 | 1 | 1 | 9 | 5 | 7 | 3 | 10 | 7 | 13 | 7 | 10 | 8 | 8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 8 | 2 | 17 | 7 | 9 | 15 | 10 | 12 | 7 | 6 | 4 | 7 | 9 | 9 | 6 | 5 | 16 | 0 | 2 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 1 | 2 | 2 | 2 | 4 | 5 | 4 | 0 | 7 | 10 | 1 | 1 | 0 | 4 | 5 | 4 | 0 | 0 | 0 | 0 | 1 | 2 |
Endosome | 21 | 7 | 3 | 1 | 9 | 3 | 6 | 3 | 0 | 0 | 0 | 2 | 14 | 10 | 7 | 19 | 9 | 9 | 13 | 17 | 17 | 11 | 8 | 5 |
Golgi | 30 | 1 | 0 | 0 | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 4 | 23 | 4 | 22 | 42 | 28 | 14 | 17 | 7 | 19 | 36 | 25 |
Mitochondria | 192 | 269 | 325 | 297 | 334 | 385 | 474 | 527 | 384 | 383 | 320 | 499 | 202 | 264 | 292 | 8 | 19 | 11 | 242 | 233 | 292 | 152 | 143 | 164 |
Nucleus | 1 | 1 | 1 | 0 | 2 | 2 | 5 | 3 | 5 | 4 | 1 | 2 | 1 | 1 | 0 | 3 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 16 | 15 | 14 | 11 | 21 | 39 | 61 | 59 | 47 | 39 | 40 | 63 | 4 | 1 | 7 | 7 | 2 | 0 | 2 | 2 | 2 | 1 | 2 | 1 |
Nucleolus | 19 | 7 | 8 | 6 | 19 | 28 | 49 | 42 | 29 | 28 | 25 | 34 | 3 | 5 | 3 | 9 | 7 | 4 | 1 | 0 | 0 | 1 | 1 | 4 |
Peroxisomes | 19 | 11 | 19 | 6 | 15 | 6 | 5 | 6 | 0 | 6 | 5 | 2 | 13 | 24 | 8 | 48 | 38 | 26 | 14 | 11 | 19 | 12 | 20 | 31 |
SpindlePole | 5 | 1 | 5 | 1 | 1 | 6 | 6 | 2 | 1 | 2 | 3 | 6 | 5 | 2 | 2 | 28 | 22 | 12 | 1 | 1 | 2 | 0 | 0 | 0 |
Vac/Vac Membrane | 42 | 46 | 25 | 9 | 13 | 10 | 19 | 24 | 20 | 13 | 18 | 15 | 26 | 25 | 31 | 39 | 28 | 15 | 1 | 15 | 2 | 6 | 5 | 3 |
Unique Cell Count | 260 | 307 | 342 | 320 | 370 | 410 | 525 | 563 | 416 | 412 | 337 | 519 | 238 | 304 | 322 | 173 | 155 | 117 | 297 | 315 | 350 | 217 | 238 | 261 |
Labelled Cell Count | 354 | 381 | 430 | 376 | 450 | 509 | 666 | 709 | 521 | 507 | 449 | 678 | 290 | 385 | 381 | 216 | 192 | 138 | 297 | 315 | 350 | 217 | 238 | 261 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.1 | 7.7 | 5.7 | 6.1 | 7.2 | 7.2 | 7.0 | 7.0 | 6.5 | 6.3 | 6.1 | 5.9 | 8.6 | 7.9 | 6.8 | 9.2 | 10.5 | 10.5 | 9.1 | 9.5 | 10.1 |
Std Deviation (1e-4) | 1.9 | 1.7 | 1.0 | 1.4 | 1.4 | 1.7 | 1.8 | 1.6 | 1.9 | 1.9 | 1.6 | 1.6 | 6.5 | 1.4 | 1.4 | 1.9 | 2.6 | 2.7 | 2.0 | 2.3 | 2.2 |
Intensity Change (Log2) | – | – | – | 0.11 | 0.35 | 0.35 | 0.3 | 0.29 | 0.2 | 0.16 | 0.1 | 0.05 | 0.6 | 0.47 | 0.27 | 0.69 | 0.88 | 0.89 | 0.67 | 0.75 | 0.84 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.4 | -0.6 | -1.1 | -1.6 | -2.0 | -2.1 | -1.3 | -0.1 | 0.2 | -2.4 | -2.6 | -1.7 | 0.4 | -2.8 | -0.3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.2 | 0 | 2.8 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.6 | 0 | 1.8 | 2.4 | 0 | 2.3 | 0 | 0 | 0 | 0 | 2.3 | 2.2 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 2.2 | 0 | 5.4 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5.2 | 0 | 6.7 | 10.1 | 9.3 |
Mitochondria | -1.2 | -2.4 | -0.7 | -2.5 | -0.9 | -1.5 | -1.2 | 0 | 0.8 | -4.2 | -3.7 | -2.2 | -20.3 | -18.5 | -18.1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -0.4 | 1.0 | 2.9 | 3.9 | 3.4 | 3.6 | 2.9 | 3.7 | 4.0 | -1.6 | -3.2 | -1.4 | 0 | 0 | 0 |
Nucleolus | -0.4 | 2.0 | 2.9 | 4.1 | 3.3 | 2.9 | 2.9 | 3.1 | 2.8 | 0 | -0.6 | -1.4 | 1.7 | 0 | 0 |
Peroxisomes | -2.5 | -0.9 | -3.1 | -4.0 | -4.0 | -4.9 | -3.1 | -2.9 | -4.8 | 0 | 1.2 | -2.0 | 7.1 | 6.1 | 5.2 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.4 | 5.8 | 0 |
Vacuole | -2.6 | -2.3 | -3.2 | -2.4 | -2.0 | -1.5 | -2.6 | -1.1 | -3.0 | 1.5 | 0.4 | 1.1 | 4.9 | 3.6 | 1.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.2608 | 7.7519 | 6.9814 | 6.7797 | 6.4527 | 7.2195 | 8.4353 | 9.2094 | 8.6326 | 8.1809 | 7.1004 | 8.6207 | 10.4799 | 10.4481 | 10.308 | 9.9381 | 10.086 | 9.873 |
Actin | 0.0172 | 0.0043 | 0.0068 | 0.0005 | 0.0124 | 0.0033 | 0.0161 | 0.0019 | 0.0034 | 0.0018 | 0.0037 | 0.0012 | 0.0183 | 0.0004 | 0.0007 | 0.0071 | 0.001 | 0.0014 |
Bud | 0.0002 | 0.0006 | 0.0001 | 0 | 0.0043 | 0 | 0.0002 | 0.0001 | 0.0004 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0 |
Bud Neck | 0.0003 | 0.0002 | 0.0011 | 0 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 |
Bud Periphery | 0.0006 | 0.0016 | 0.0002 | 0.0001 | 0.0204 | 0.0001 | 0.0003 | 0.0003 | 0.001 | 0.0024 | 0.0002 | 0.0005 | 0.0003 | 0 | 0.0001 | 0.0008 | 0.0003 | 0.0001 |
Bud Site | 0.0005 | 0.0019 | 0.0006 | 0 | 0.0012 | 0.0001 | 0.0005 | 0.0002 | 0.0013 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Cell Periphery | 0.0002 | 0.0059 | 0.0001 | 0 | 0.0006 | 0 | 0.0002 | 0.0002 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0004 | 0 |
Cytoplasm | 0.0099 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0007 | 0 | 0.0007 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 |
Cytoplasmic Foci | 0.0065 | 0.0011 | 0.008 | 0.0001 | 0.0158 | 0.0004 | 0.0065 | 0.0012 | 0.0099 | 0.0029 | 0.0013 | 0.0006 | 0.0043 | 0.0003 | 0.0013 | 0.0005 | 0.0007 | 0.0006 |
Eisosomes | 0.0003 | 0.0007 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0002 |
Endosome | 0.0078 | 0.0036 | 0.0086 | 0.0002 | 0.003 | 0.0012 | 0.0099 | 0.0042 | 0.0131 | 0.0129 | 0.0056 | 0.0028 | 0.0041 | 0.0013 | 0.0035 | 0.0002 | 0.0023 | 0.0033 |
Golgi | 0.0068 | 0.0083 | 0.008 | 0.0005 | 0.0063 | 0.004 | 0.0098 | 0.0171 | 0.0134 | 0.0078 | 0.017 | 0.0088 | 0.0106 | 0.0019 | 0.0139 | 0.0015 | 0.0148 | 0.0104 |
Lipid Particles | 0.0322 | 0.0063 | 0.0061 | 0.0004 | 0.0145 | 0.0044 | 0.0288 | 0.0128 | 0.0144 | 0.0142 | 0.0112 | 0.0048 | 0.0082 | 0.0006 | 0.0009 | 0.0002 | 0.012 | 0.0082 |
Mitochondria | 0.8818 | 0.9456 | 0.9442 | 0.9956 | 0.8535 | 0.9802 | 0.8811 | 0.9459 | 0.9221 | 0.9491 | 0.9533 | 0.9711 | 0.9419 | 0.9893 | 0.976 | 0.9834 | 0.9405 | 0.9667 |
None | 0.0009 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0018 | 0 | 0.0005 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0002 |
Nuclear Periphery | 0.0018 | 0 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0023 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0015 | 0.0001 | 0 | 0 | 0 | 0.0008 | 0.0011 |
Nucleolus | 0.0002 | 0.0004 | 0.0003 | 0 | 0.0002 | 0 | 0.0012 | 0.0003 | 0.0009 | 0.0001 | 0.0001 | 0.0002 | 0.0005 | 0 | 0 | 0 | 0.005 | 0.0001 |
Nucleus | 0.0005 | 0.0002 | 0.0001 | 0 | 0.0003 | 0 | 0.0052 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0007 | 0.0002 | 0 | 0 | 0 | 0.0014 | 0.0001 |
Peroxisomes | 0.0208 | 0.0099 | 0.0102 | 0.0016 | 0.0557 | 0.0023 | 0.0227 | 0.0067 | 0.0081 | 0.0032 | 0.0043 | 0.0025 | 0.0037 | 0.0051 | 0.0019 | 0.0059 | 0.001 | 0.0048 |
Punctate Nuclear | 0.0017 | 0 | 0.0004 | 0 | 0.0005 | 0.0002 | 0.0019 | 0 | 0.0037 | 0.0001 | 0 | 0.0002 | 0.0021 | 0 | 0 | 0 | 0 | 0.0003 |
Vacuole | 0.0021 | 0.0062 | 0.0017 | 0.0001 | 0.0049 | 0.0002 | 0.0043 | 0.0045 | 0.0024 | 0.0009 | 0.0005 | 0.0013 | 0.0013 | 0.0002 | 0.0003 | 0 | 0.0036 | 0.0004 |
Vacuole Periphery | 0.0058 | 0.0029 | 0.0031 | 0.0006 | 0.0053 | 0.0031 | 0.006 | 0.004 | 0.003 | 0.0025 | 0.0021 | 0.0033 | 0.0028 | 0.0006 | 0.0012 | 0.0001 | 0.0155 | 0.002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 19.1331 | 17.602 | 18.9761 | 27.1693 | 17.3408 | 29.7049 | 31.9849 | 33.7696 | 32.0016 | 27.5733 |
Translational Efficiency | 1.0345 | 0.9221 | 0.927 | 0.6688 | 0.705 | 0.826 | 0.7607 | 0.6814 | 0.7949 | 0.7863 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
736 | 261 | 774 | 717 | 313 | 1642 | 114 | 147 | 1049 | 1903 | 888 | 864 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 800.31 | 1115.12 | 1436.26 | 1242.18 | 1014.94 | 965.49 | 1377.98 | 1383.79 | 864.35 | 986.01 | 1428.78 | 1266.27 |
Standard Deviation | 110.82 | 172.35 | 224.78 | 224.22 | 158.08 | 160.52 | 183.65 | 220.95 | 160.36 | 170.17 | 220.79 | 229.91 |
Intensity Change Log 2 | — | 0.478568 | 0.843686 | 0.634243 | — | -0.072061 | 0.441161 | 0.447231 | — | 0.196838 | 0.632577 | 0.532682 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000914 | 0.002106 | 0.006668 | 0.005900 | 0.000993 | 0.002723 | 0.012426 | 0.009431 | 0.000938 | 0.002638 | 0.007408 | 0.006501 |
Bud Neck | 0.002405 | 0.002145 | 0.004230 | 0.003671 | 0.002306 | 0.000723 | 0.015537 | 0.002547 | 0.002376 | 0.000918 | 0.005681 | 0.003480 |
Bud Site | 0.004698 | 0.004734 | 0.003661 | 0.010933 | 0.001284 | 0.001257 | 0.016759 | 0.004137 | 0.003679 | 0.001734 | 0.005343 | 0.009777 |
Cell Periphery | 0.000519 | 0.000135 | 0.000106 | 0.000520 | 0.000066 | 0.000123 | 0.000439 | 0.000350 | 0.000384 | 0.000125 | 0.000149 | 0.000491 |
Cytoplasm | 0.001082 | 0.000522 | 0.000500 | 0.001155 | 0.000163 | 0.000401 | 0.001128 | 0.000626 | 0.000808 | 0.000418 | 0.000580 | 0.001065 |
Cytoplasmic Foci | 0.015463 | 0.035927 | 0.036909 | 0.018301 | 0.011182 | 0.020355 | 0.082648 | 0.016819 | 0.014186 | 0.022490 | 0.042781 | 0.018049 |
Eisosomes | 0.000065 | 0.000121 | 0.000194 | 0.000271 | 0.000061 | 0.000086 | 0.000274 | 0.000270 | 0.000064 | 0.000090 | 0.000205 | 0.000271 |
Endoplasmic Reticulum | 0.000432 | 0.000360 | 0.000321 | 0.001162 | 0.000083 | 0.000114 | 0.001203 | 0.000658 | 0.000328 | 0.000148 | 0.000434 | 0.001076 |
Endosome | 0.030456 | 0.029334 | 0.011269 | 0.014394 | 0.010591 | 0.021900 | 0.046154 | 0.015934 | 0.024529 | 0.022920 | 0.015748 | 0.014656 |
Golgi | 0.018406 | 0.053271 | 0.046974 | 0.056397 | 0.016062 | 0.033527 | 0.075636 | 0.078116 | 0.017707 | 0.036235 | 0.050653 | 0.060092 |
Lipid Particles | 0.008120 | 0.008255 | 0.007942 | 0.003403 | 0.003460 | 0.004552 | 0.013200 | 0.001732 | 0.006730 | 0.005060 | 0.008617 | 0.003118 |
Mitochondria | 0.867218* | 0.816803* | 0.807531* | 0.826985* | 0.931813* | 0.882363* | 0.560842* | 0.790088* | 0.886492* | 0.873371* | 0.775862* | 0.820708* |
Mitotic Spindle | 0.002903 | 0.003066 | 0.018390 | 0.004159 | 0.001566 | 0.002102 | 0.066012 | 0.004985 | 0.002504 | 0.002234 | 0.024503 | 0.004300 |
None | 0.000459 | 0.000396 | 0.001274 | 0.000855 | 0.000481 | 0.000253 | 0.000627 | 0.001189 | 0.000465 | 0.000273 | 0.001191 | 0.000912 |
Nuclear Periphery | 0.000151 | 0.000418 | 0.002293 | 0.000463 | 0.000202 | 0.000114 | 0.003065 | 0.000748 | 0.000166 | 0.000156 | 0.002392 | 0.000512 |
Nuclear Periphery Foci | 0.000378 | 0.001023 | 0.001354 | 0.000375 | 0.000194 | 0.000168 | 0.002893 | 0.000507 | 0.000323 | 0.000286 | 0.001552 | 0.000398 |
Nucleolus | 0.000660 | 0.000251 | 0.000784 | 0.000945 | 0.000104 | 0.000069 | 0.001429 | 0.000359 | 0.000494 | 0.000094 | 0.000867 | 0.000846 |
Nucleus | 0.000610 | 0.000207 | 0.000261 | 0.000239 | 0.000070 | 0.000048 | 0.000686 | 0.000074 | 0.000448 | 0.000070 | 0.000316 | 0.000211 |
Peroxisomes | 0.026397 | 0.024236 | 0.021091 | 0.020491 | 0.015823 | 0.022511 | 0.053774 | 0.027367 | 0.023242 | 0.022747 | 0.025287 | 0.021661 |
Vacuole | 0.007864 | 0.007860 | 0.011478 | 0.014763 | 0.001669 | 0.003559 | 0.026707 | 0.019228 | 0.006015 | 0.004149 | 0.013433 | 0.015523 |
Vacuole Periphery | 0.010798 | 0.008831 | 0.016768 | 0.014618 | 0.001829 | 0.003050 | 0.018562 | 0.024835 | 0.008122 | 0.003843 | 0.016998 | 0.016356 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.84 | -4.76 | -3.85 | -2.82 | 0.43 | -2.49 | -2.61 | -2.45 | -1.96 | 0.55 | -4.36 | -5.38 | -4.52 | -3.07 | 0.53 |
Bud Neck | 0.36 | -2.99 | -1.56 | -1.69 | 0.63 | 0.99 | -3.95 | -0.15 | -3.59 | 4.35 | 2.55 | -4.20 | -1.38 | -4.18 | 2.64 |
Bud Site | -0.02 | 0.72 | -2.65 | -1.99 | -3.18 | 0.02 | -2.48 | -1.76 | -2.14 | 2.01 | 2.10 | -1.24 | -3.14 | -4.37 | -2.16 |
Cell Periphery | 2.45 | 2.64 | -0.01 | -3.21 | -3.47 | -3.87 | -2.72 | -5.04 | -3.98 | 0.60 | 2.35 | 2.11 | -0.74 | -3.68 | -3.39 |
Cytoplasm | 1.55 | 1.65 | -0.23 | -2.51 | -2.74 | -4.15 | -4.65 | -3.14 | -1.48 | 2.00 | 1.56 | 0.91 | -0.86 | -3.19 | -2.41 |
Cytoplasmic Foci | -3.77 | -6.34 | -1.02 | 3.20 | 5.28 | -3.50 | -6.25 | -1.23 | 0.82 | 5.51 | -4.06 | -8.80 | -1.64 | 1.88 | 7.16 |
Eisosomes | -3.47 | -6.71 | -3.50 | -2.49 | -1.26 | -2.45 | -2.83 | -4.12 | -3.66 | 0.04 | -3.52 | -7.34 | -4.18 | -3.65 | -1.27 |
Endoplasmic Reticulum | 0.46 | 0.90 | -1.59 | -1.75 | -1.88 | -1.51 | -4.13 | -3.44 | -3.26 | 1.72 | 2.22 | -1.09 | -1.97 | -2.50 | -1.71 |
Endosome | 0.23 | 6.20 | 4.84 | 3.60 | -1.66 | -4.09 | -4.66 | -1.29 | 1.53 | 3.72 | 0.61 | 3.42 | 3.79 | 3.93 | 0.55 |
Golgi | -4.84 | -6.37 | -6.77 | -0.36 | -1.44 | -4.14 | -4.57 | -4.51 | -3.31 | -0.14 | -6.59 | -7.79 | -8.15 | -4.49 | -1.53 |
Lipid Particles | -0.04 | 0.09 | 2.65 | 1.72 | 3.11 | -0.86 | -2.96 | 1.49 | 3.21 | 3.62 | 1.23 | -1.16 | 2.70 | 1.96 | 4.17 |
Mitochondria | 2.53 | 4.40 | 2.88 | -0.46 | -1.29 | 4.14 | 9.85 | 5.26 | 3.63 | -5.23 | 1.44 | 8.81 | 5.41 | 4.69 | -3.11 |
Mitotic Spindle | -0.12 | -5.72 | -1.04 | -1.00 | 5.49 | -0.60 | -4.32 | -1.94 | -1.73 | 4.08 | 0.31 | -7.23 | -1.87 | -2.77 | 6.71 |
None | 0.98 | -7.86 | -3.47 | -4.11 | 3.02 | 2.69 | -1.32 | -4.39 | -6.74 | -3.61 | 4.36 | -7.96 | -4.53 | -7.08 | 2.31 |
Nuclear Periphery | -1.51 | -4.79 | -2.98 | -0.23 | 4.00 | 1.05 | -4.43 | -2.04 | -2.43 | 3.35 | 0.18 | -5.58 | -3.51 | -3.50 | 4.61 |
Nuclear Periphery Foci | -1.17 | -4.46 | 0.03 | 1.18 | 4.47 | 0.32 | -4.53 | -1.87 | -1.97 | 3.88 | 0.36 | -6.06 | -0.84 | -0.98 | 5.58 |
Nucleolus | 1.38 | -0.37 | -0.60 | -1.57 | -0.35 | 0.67 | -2.63 | -1.98 | -2.34 | 2.07 | 2.25 | -1.39 | -0.92 | -2.17 | 0.04 |
Nucleus | 1.75 | 1.48 | 1.61 | -0.25 | 0.16 | 0.54 | -3.89 | -0.13 | -1.15 | 3.95 | 2.54 | 0.75 | 1.42 | -1.78 | 0.86 |
Peroxisomes | 0.39 | 1.25 | 1.30 | 0.62 | 0.16 | -1.57 | -3.61 | -1.16 | -0.51 | 1.97 | 0.19 | -0.55 | 0.41 | 0.29 | 0.88 |
Vacuole | -0.01 | -2.17 | -2.89 | -2.37 | -1.31 | -2.69 | -4.24 | -2.86 | -2.55 | 0.88 | 2.08 | -4.69 | -4.30 | -5.21 | -0.87 |
Vacuole Periphery | 0.60 | -2.73 | -1.58 | -1.65 | 0.82 | -3.16 | -5.14 | -3.77 | -3.57 | -0.91 | 3.81 | -4.91 | -3.77 | -6.28 | 0.25 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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