Standard name | |
---|---|
Human Ortholog | |
Description | Transmembrane protein; subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 10 | 8 | 4 | 5 | 7 | 6 | 20 | 9 | 12 | 9 | 9 | 5 | 10 | 5 | 26 | 18 | 22 | 2 | 6 | 5 | 1 | 5 | 8 |
Bud | 0 | 0 | 2 | 2 | 5 | 8 | 16 | 7 | 6 | 16 | 6 | 3 | 0 | 0 | 0 | 2 | 0 | 2 | 1 | 5 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 1 | 1 | 1 | 3 | 8 | 6 | 12 | 12 | 25 | 13 | 11 | 4 | 31 | 16 | 40 | 30 | 40 | 0 | 0 | 0 | 1 | 4 | 9 |
Cytoplasm | 18 | 36 | 34 | 13 | 27 | 46 | 27 | 45 | 35 | 58 | 27 | 25 | 13 | 31 | 27 | 100 | 49 | 36 | 0 | 10 | 5 | 3 | 2 | 4 |
Endoplasmic Reticulum | 24 | 191 | 94 | 84 | 72 | 70 | 69 | 117 | 97 | 126 | 67 | 75 | 65 | 146 | 90 | 14 | 1 | 4 | 136 | 242 | 141 | 55 | 113 | 129 |
Endosome | 6 | 1 | 1 | 0 | 5 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 2 | 1 | 0 | 4 | 2 | 0 | 1 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 39 | 13 | 0 | 5 | 1 | 0 | 1 | 1 |
Mitochondria | 23 | 8 | 40 | 46 | 55 | 129 | 156 | 112 | 100 | 195 | 83 | 89 | 2 | 5 | 7 | 22 | 13 | 8 | 4 | 4 | 11 | 2 | 2 | 5 |
Nucleus | 0 | 1 | 2 | 0 | 1 | 3 | 2 | 3 | 4 | 4 | 0 | 2 | 0 | 1 | 0 | 2 | 2 | 2 | 0 | 0 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 1 | 9 | 9 | 13 | 9 | 13 | 16 | 15 | 15 | 15 | 7 | 7 | 2 | 5 | 2 | 1 | 3 | 0 | 2 | 4 | 6 | 0 | 1 | 0 |
Nucleolus | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 45 | 35 | 19 | 7 | 61 | 49 | 54 | 33 | 30 | 46 | 16 | 14 | 0 | 3 | 8 | 104 | 63 | 41 | 2 | 9 | 7 | 2 | 4 | 4 |
Unique Cell Count | 104 | 255 | 169 | 129 | 191 | 247 | 260 | 283 | 223 | 353 | 164 | 166 | 81 | 190 | 131 | 293 | 189 | 148 | 152 | 299 | 188 | 72 | 143 | 169 |
Labelled Cell Count | 122 | 292 | 211 | 170 | 244 | 335 | 356 | 366 | 309 | 500 | 228 | 237 | 91 | 232 | 155 | 336 | 222 | 169 | 152 | 299 | 188 | 72 | 143 | 169 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.9 | 9.1 | 7.1 | 6.8 | 7.1 | 6.5 | 5.8 | 6.9 | 6.8 | 6.3 | 6.4 | 6.6 | 9.0 | 9.2 | 8.7 | 9.6 | 11.9 | 12.0 | 7.7 | 8.7 | 9.7 |
Std Deviation (1e-4) | 1.9 | 2.2 | 1.9 | 1.6 | 1.5 | 1.6 | 1.4 | 1.6 | 1.5 | 1.4 | 1.3 | 1.7 | 2.0 | 2.2 | 2.4 | 2.6 | 3.0 | 3.0 | 1.5 | 2.1 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.0 | -0.14 | -0.3 | -0.06 | -0.08 | -0.17 | -0.17 | -0.11 | 0.34 | 0.37 | 0.28 | 0.43 | 0.74 | 0.75 | 0.12 | 0.28 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.7 | -1.1 | -1.0 | -1.4 | 1.0 | -0.3 | -0.7 | 0.3 | 0.3 | 0 | 0.2 | -0.4 | 1.6 | 1.7 | 3.1 |
Bud | 0 | 0 | 0 | 2.5 | 0 | 0 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 2.6 | 3.2 | 3.3 | 3.0 | 0 | 5.2 | 4.3 | 4.8 | 5.1 | 7.0 |
Cytoplasm | -2.4 | -1.5 | -0.4 | -2.8 | -1.1 | -1.1 | -1.0 | -0.9 | -1.2 | -0.8 | -0.9 | 0.1 | 3.2 | 1.3 | 0.9 |
Endoplasmic Reticulum | 1.7 | -3.4 | -5.6 | -6.1 | -2.9 | -2.4 | -4.3 | -2.7 | -1.9 | 3.8 | 4.3 | 2.3 | -12.4 | -11.8 | -10.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.3 | 3.9 |
Mitochondria | 2.3 | 1.1 | 5.8 | 7.4 | 3.5 | 4.3 | 6.8 | 5.1 | 5.6 | -4.2 | -6.0 | -4.3 | -4.9 | -4.5 | -4.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1.6 | -0.3 | 0 | 0.4 | 0 | 0.6 | -0.5 | -0.5 | -0.5 | 0 | -1.3 | 0 | 0 | -2.0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.8 | 4.7 | 2.3 | 2.6 | 0.1 | 0.7 | 0.6 | -0.4 | -0.9 | -3.1 | -3.8 | -1.5 | 5.7 | 5.0 | 3.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.7751 | 5.174 | 5.0691 | 5.8209 | 9.1021 | 5.151 | 3.6614 | 4.6706 | 4.7568 | 4.1224 | 3.1993 | 5.0494 | 5.4307 | 5.4348 | 5.609 | 5.4337 | 5.5714 | 5.8577 |
Actin | 0 | 0.0012 | 0.0011 | 0.0006 | 0.0023 | 0.0046 | 0.002 | 0.0021 | 0.029 | 0.0123 | 0.0474 | 0.0035 | 0.0063 | 0.0117 | 0.0162 | 0.0067 | 0.0029 | 0.0132 |
Bud | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0004 | 0.0003 | 0.0002 | 0.0004 | 0 |
Bud Neck | 0 | 0.0006 | 0.0092 | 0.0001 | 0.0002 | 0.003 | 0.0002 | 0.0001 | 0.0005 | 0.002 | 0.0007 | 0.0004 | 0.0006 | 0.0014 | 0.0004 | 0.0003 | 0.0007 | 0.0004 |
Bud Periphery | 0 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0.0004 | 0.0002 | 0.0004 | 0.0001 | 0.0005 | 0.0015 | 0.0007 | 0.0003 | 0.0006 | 0.0001 |
Bud Site | 0 | 0.008 | 0.0004 | 0 | 0.0002 | 0.0002 | 0.0003 | 0.0017 | 0.0034 | 0.0006 | 0.0016 | 0 | 0.0024 | 0.0171 | 0.0009 | 0.0004 | 0.0023 | 0 |
Cell Periphery | 0.0001 | 0.0159 | 0.0055 | 0.0003 | 0.0004 | 0.0025 | 0.0005 | 0.004 | 0.0024 | 0.0006 | 0.0005 | 0.0003 | 0.0008 | 0.0032 | 0.0012 | 0.0007 | 0.0004 | 0.0005 |
Cytoplasm | 0.0012 | 0.2391 | 0.1763 | 0.033 | 0.0138 | 0.0836 | 0.0556 | 0.2001 | 0.0415 | 0.165 | 0.1312 | 0.0629 | 0.1263 | 0.1249 | 0.0792 | 0.1127 | 0.0911 | 0.0801 |
Cytoplasmic Foci | 0.0001 | 0.0169 | 0.0032 | 0.0008 | 0.0019 | 0.0038 | 0.0241 | 0.0058 | 0.0069 | 0.0344 | 0.0307 | 0.0061 | 0.0277 | 0.0108 | 0.0053 | 0.0033 | 0.0332 | 0.0044 |
Eisosomes | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0003 | 0.0023 | 0.0003 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.927 | 0.5912 | 0.6696 | 0.9402 | 0.9426 | 0.8265 | 0.6739 | 0.612 | 0.7589 | 0.5579 | 0.5077 | 0.7731 | 0.6654 | 0.725 | 0.8084 | 0.7739 | 0.6019 | 0.82 |
Endosome | 0.0035 | 0.087 | 0.0041 | 0.0047 | 0.0115 | 0.0061 | 0.0362 | 0.0059 | 0.0381 | 0.0459 | 0.0406 | 0.0172 | 0.0408 | 0.0114 | 0.0093 | 0.0339 | 0.0895 | 0.0047 |
Golgi | 0.0001 | 0.0047 | 0.0011 | 0.0003 | 0.0025 | 0.0017 | 0.036 | 0.0012 | 0.0195 | 0.0646 | 0.0502 | 0.0137 | 0.012 | 0.0025 | 0.0042 | 0.0111 | 0.0225 | 0.0005 |
Lipid Particles | 0.0014 | 0.0157 | 0.006 | 0.0008 | 0.0019 | 0.0071 | 0.0317 | 0.0062 | 0.0065 | 0.0075 | 0.0536 | 0.0066 | 0.0137 | 0.0099 | 0.0066 | 0.0033 | 0.0324 | 0.0043 |
Mitochondria | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0009 | 0.0004 | 0.0033 | 0.0005 | 0.0177 | 0.0094 | 0.0802 | 0.0026 | 0.0042 | 0.001 | 0.0013 | 0.0094 | 0.0045 | 0.0001 |
None | 0.0004 | 0.0011 | 0.1103 | 0.0013 | 0.0005 | 0.0014 | 0.0869 | 0.142 | 0.0474 | 0.0668 | 0.0193 | 0.0802 | 0.0233 | 0.0139 | 0.0317 | 0.0213 | 0.032 | 0.0416 |
Nuclear Periphery | 0.0606 | 0.0106 | 0.0076 | 0.0132 | 0.0169 | 0.0447 | 0.0129 | 0.0081 | 0.0125 | 0.0077 | 0.0082 | 0.0262 | 0.0404 | 0.0405 | 0.0256 | 0.0152 | 0.0457 | 0.0247 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0 |
Nucleus | 0.0005 | 0.0012 | 0.0013 | 0.0013 | 0.0009 | 0.0017 | 0.0009 | 0.001 | 0.001 | 0.001 | 0.0008 | 0.0012 | 0.0091 | 0.0083 | 0.0023 | 0.0012 | 0.0064 | 0.0016 |
Peroxisomes | 0 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0111 | 0.0001 | 0.0063 | 0.0191 | 0.0193 | 0.0004 | 0.0109 | 0.0034 | 0.0008 | 0.0001 | 0.0023 | 0.0001 |
Punctate Nuclear | 0.0001 | 0.0012 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0206 | 0.0006 | 0.0006 | 0.001 | 0.0017 | 0.0004 | 0.0027 | 0.0032 | 0.0011 | 0.0003 | 0.018 | 0.0003 |
Vacuole | 0.0026 | 0.0046 | 0.0027 | 0.0025 | 0.0022 | 0.0102 | 0.0023 | 0.0048 | 0.0047 | 0.0028 | 0.0034 | 0.0037 | 0.0068 | 0.0067 | 0.0027 | 0.0038 | 0.0104 | 0.0027 |
Vacuole Periphery | 0.0022 | 0.0005 | 0.0002 | 0.0004 | 0.0007 | 0.0015 | 0.0007 | 0.0004 | 0.0016 | 0.0007 | 0.0011 | 0.0013 | 0.005 | 0.0025 | 0.0014 | 0.0017 | 0.0024 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 28.8741 | 17.2078 | 19.6094 | 34.5569 | 24.9577 | 20.613 | 33.8655 | 35.8581 | 29.7731 | 43.5309 |
Translational Efficiency | 1.4649 | 1.8815 | 1.6278 | 0.7706 | 1.201 | 1.7209 | 1.0876 | 0.6825 | 0.9684 | 0.7988 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2466 | 1319 | 1728 | 1679 | 587 | 2238 | 81 | 82 | 3053 | 3557 | 1809 | 1761 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 715.43 | 749.83 | 916.68 | 917.55 | 751.75 | 820.45 | 998.85 | 995.19 | 722.41 | 794.26 | 920.36 | 921.17 |
Standard Deviation | 81.24 | 113.13 | 146.24 | 109.88 | 84.01 | 111.06 | 102.22 | 121.75 | 83.02 | 116.92 | 145.55 | 111.66 |
Intensity Change Log 2 | — | 0.067753 | 0.357608 | 0.358976 | — | 0.126162 | 0.410015 | 0.404719 | — | 0.097976 | 0.384698 | 0.382595 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000312 | 0.002156 | 0.001808 | 0.016130 | 0.001488 | 0.000634 | 0.001763 | 0.014315 | 0.000538 | 0.001198 | 0.001806 | 0.016046 |
Bud Neck | 0.042805 | 0.024454 | 0.014499 | 0.027788 | 0.024508 | 0.011000 | 0.028297 | 0.018037 | 0.039287 | 0.015989 | 0.015117 | 0.027334 |
Bud Site | 0.022624 | 0.075401 | 0.053376 | 0.134412* | 0.036622 | 0.030758 | 0.053892 | 0.138499* | 0.025316 | 0.047313 | 0.053399 | 0.134602* |
Cell Periphery | 0.000433 | 0.000326 | 0.000146 | 0.000292 | 0.000616 | 0.000206 | 0.000203 | 0.000254 | 0.000468 | 0.000251 | 0.000149 | 0.000290 |
Cytoplasm | 0.112914* | 0.043361 | 0.060905 | 0.263707* | 0.045160 | 0.031366 | 0.120409* | 0.191589* | 0.099887 | 0.035814 | 0.063570 | 0.260349* |
Cytoplasmic Foci | 0.232265* | 0.119743 | 0.142985 | 0.132182 | 0.188032* | 0.128377 | 0.161444 | 0.184282* | 0.223760* | 0.125175 | 0.143812 | 0.134608 |
Eisosomes | 0.000214 | 0.000121 | 0.000064 | 0.000185 | 0.000223 | 0.000089 | 0.000172 | 0.000170 | 0.000216 | 0.000101 | 0.000069 | 0.000185 |
Endoplasmic Reticulum | 0.004186 | 0.001645 | 0.005628 | 0.004166 | 0.001690 | 0.000661 | 0.005024 | 0.002827 | 0.003706 | 0.001026 | 0.005601 | 0.004104 |
Endosome | 0.085354 | 0.098947 | 0.081786 | 0.023152 | 0.100802 | 0.096661 | 0.098226 | 0.040691 | 0.088324 | 0.097509 | 0.082522 | 0.023968 |
Golgi | 0.054499 | 0.093339 | 0.067464 | 0.022632 | 0.102898 | 0.150650* | 0.006251 | 0.013062 | 0.063805 | 0.129398* | 0.064723 | 0.022186 |
Lipid Particles | 0.024502 | 0.004665 | 0.003229 | 0.002981 | 0.007680 | 0.002366 | 0.008567 | 0.002834 | 0.021268 | 0.003219 | 0.003468 | 0.002974 |
Mitochondria | 0.248737* | 0.344136* | 0.134895* | 0.040255 | 0.345103* | 0.429900* | 0.023684 | 0.002612 | 0.267265* | 0.398098* | 0.129916* | 0.038502 |
Mitotic Spindle | 0.004165 | 0.022877 | 0.127027* | 0.056636 | 0.012055 | 0.020647 | 0.096381 | 0.082647 | 0.005682 | 0.021474 | 0.125655* | 0.057847 |
None | 0.002074 | 0.001106 | 0.001089 | 0.000651 | 0.000557 | 0.000670 | 0.001214 | 0.000328 | 0.001783 | 0.000831 | 0.001095 | 0.000636 |
Nuclear Periphery | 0.000733 | 0.000472 | 0.003173 | 0.000817 | 0.000252 | 0.000184 | 0.001445 | 0.000461 | 0.000640 | 0.000291 | 0.003095 | 0.000801 |
Nuclear Periphery Foci | 0.000505 | 0.000768 | 0.002679 | 0.001343 | 0.000306 | 0.000191 | 0.002639 | 0.001478 | 0.000467 | 0.000405 | 0.002678 | 0.001349 |
Nucleolus | 0.002681 | 0.002711 | 0.003184 | 0.000587 | 0.001412 | 0.000598 | 0.005630 | 0.000406 | 0.002437 | 0.001381 | 0.003293 | 0.000578 |
Nucleus | 0.028054 | 0.010325 | 0.025866 | 0.051454 | 0.009175 | 0.002372 | 0.058910 | 0.026629 | 0.024424 | 0.005321 | 0.027345 | 0.050298 |
Peroxisomes | 0.017994 | 0.031757 | 0.013646 | 0.058480 | 0.039830 | 0.018754 | 0.033156 | 0.072125 | 0.022193 | 0.023576 | 0.014520 | 0.059115 |
Vacuole | 0.087109 | 0.081101 | 0.194460 | 0.155983 | 0.045776 | 0.043697 | 0.274877* | 0.205533* | 0.079162 | 0.057567 | 0.198061 | 0.158291 |
Vacuole Periphery | 0.027839 | 0.040589 | 0.062088 | 0.006168 | 0.035817 | 0.030219 | 0.017817 | 0.001222 | 0.029373 | 0.034064 | 0.060106 | 0.005938 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -4.98 | -10.82 | -10.22 | -8.39 | -8.93 | 3.21 | -1.19 | -1.54 | -1.69 | -1.44 | -4.27 | -9.12 | -10.17 | -9.57 | -9.03 |
Bud Neck | 6.44 | 12.24 | 7.06 | -0.50 | -6.35 | 4.94 | -0.60 | 2.12 | -1.38 | 2.08 | 11.99 | 11.94 | 6.58 | -5.89 | -5.91 |
Bud Site | -13.68 | -10.89 | -19.89 | -6.11 | -11.21 | 1.52 | -1.58 | -3.17 | -3.50 | -2.21 | -10.45 | -10.22 | -19.60 | -13.75 | -11.43 |
Cell Periphery | 0.88 | 2.36 | 1.42 | 2.43 | -5.81 | 3.25 | 3.24 | 2.96 | -0.36 | -0.33 | 2.12 | 3.15 | 2.06 | -0.29 | -5.81 |
Cytoplasm | 15.10 | 10.53 | -22.24 | -33.41 | -30.17 | 3.15 | -4.62 | -5.22 | -5.79 | -1.65 | 18.70 | 7.79 | -24.84 | -37.05 | -29.73 |
Cytoplasmic Foci | 20.38 | 17.19 | 22.22 | 1.21 | 5.38 | 7.61 | 2.74 | 2.10 | -0.92 | -0.17 | 22.98 | 16.88 | 21.90 | 2.38 | 5.19 |
Eisosomes | 7.68 | 13.75 | 3.12 | -3.90 | -9.63 | 5.88 | 1.16 | 1.73 | -1.98 | 0.83 | 11.41 | 14.65 | 3.51 | -6.33 | -9.34 |
Endoplasmic Reticulum | 6.17 | -3.07 | -0.58 | -7.87 | 2.72 | 4.20 | -3.39 | -2.47 | -3.70 | 1.05 | 8.19 | -4.38 | -1.68 | -10.40 | 2.84 |
Endosome | -3.09 | 1.46 | 24.07 | 21.51 | 22.65* | 0.65 | 1.32 | 7.98 | 8.88 | 4.41 | -2.91 | 2.37 | 26.97 | 31.97 | 23.06 |
Golgi | -8.37 | -3.06 | 14.68 | 17.65 | 15.37 | -6.59 | 15.67 | 13.76 | 28.74 | -1.01 | -18.16 | 0.35 | 18.71 | 34.72 | 15.25 |
Lipid Particles | 12.60 | 15.62 | 15.86 | 1.95 | 0.64 | 5.33 | -0.75 | 3.54 | -1.82 | 3.62 | 15.65 | 15.70 | 16.21 | 0.36 | 1.28 |
Mitochondria | -9.00 | 15.94 | 35.65* | 34.31* | 18.78* | -5.83 | 21.91 | 26.33 | 60.65* | 2.16 | -17.20 | 20.24 | 41.62* | 61.46* | 18.78* |
Mitotic Spindle | -9.18 | -23.26* | -13.40 | -7.05 | 12.17 | -4.05 | -3.11 | -2.61 | -2.23 | 0.40 | -12.31 | -23.17 | -13.24 | -8.33 | 11.92 |
None | 3.92 | 4.47 | 6.48 | 2.25 | 2.88 | -0.54 | -1.25 | 0.71 | 1.04 | 1.46 | 4.92 | 3.69 | 6.22 | 0.85 | 3.12 |
Nuclear Periphery | 1.35 | -5.62 | -1.90 | -2.60 | 4.31 | 0.84 | -4.63 | -2.63 | -2.92 | 2.09 | 3.27 | -5.95 | -2.39 | -4.06 | 4.37 |
Nuclear Periphery Foci | -0.92 | -7.18 | -3.51 | -1.84 | 2.93 | 1.55 | -3.88 | -4.48 | -4.68 | 1.18 | 0.47 | -7.77 | -4.00 | -3.94 | 3.04 |
Nucleolus | -0.40 | -1.13 | 6.09 | 3.90 | 6.96 | 1.89 | -1.61 | 2.20 | 1.07 | 2.21 | 2.80 | -1.83 | 6.33 | 3.42 | 7.31 |
Nucleus | 8.06 | -0.14 | -13.71 | -19.19 | -12.31 | 4.18 | -4.51 | -3.45 | -4.26 | 1.72 | 13.43 | -2.35 | -15.34 | -23.51 | -11.61 |
Peroxisomes | -5.54 | 3.29 | -9.87 | -3.92 | -11.25 | 5.34 | 0.82 | -1.01 | -2.59 | -1.36 | -0.95 | 5.40 | -8.52 | -7.83 | -11.24 |
Vacuole | 0.95 | -19.04 | -20.00 | -19.64 | -2.20 | 0.45 | -10.19 | -8.27 | -8.36 | -0.38 | 6.89 | -22.25 | -22.76 | -27.16 | -2.24 |
Vacuole Periphery | -5.38 | -12.37 | 17.43 | 16.74 | 22.78* | 1.61 | 4.87 | 10.98 | 23.15 | 4.04 | -3.00 | -11.43 | 19.43 | 23.46 | 22.88* |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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