Standard name | |
---|---|
Human Ortholog | |
Description | Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 1 | 3 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | – | 0 | 1 | 1 | 3 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 5 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 3 | – | 13 | 25 | 28 | 30 | – | 27 | 23 | 14 | 5 | 0 | 23 | 46 | 18 | 16 | 25 | 0 | 0 | 0 | 1 | 2 | 4 |
Endoplasmic Reticulum | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 6 | 2 | 6 | 0 | 0 | 0 | 0 | 1 | 0 |
Endosome | 0 | 0 | – | 0 | 3 | 4 | 3 | – | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 6 | 1 | 0 | 0 | 0 | 2 | 0 |
Golgi | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 1 |
Mitochondria | 12 | 2 | – | 1 | 0 | 27 | 51 | – | 62 | 72 | 52 | 16 | 1 | 1 | 2 | 3 | 4 | 6 | 1 | 0 | 0 | 0 | 4 | 2 |
Nucleus | 40 | 100 | – | 111 | 192 | 120 | 276 | – | 109 | 184 | 45 | 29 | 16 | 113 | 126 | 132 | 128 | 136 | 51 | 102 | 15 | 13 | 82 | 82 |
Nuclear Periphery | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 0 | – | 0 | 0 | 0 | 0 | – | 1 | 0 | 3 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 2 | 6 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | – | 2 | 7 | 5 | 13 | – | 2 | 7 | 5 | 0 | 0 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
Vac/Vac Membrane | 1 | 9 | – | 4 | 7 | 9 | 16 | – | 3 | 11 | 0 | 2 | 1 | 6 | 8 | 16 | 20 | 27 | 2 | 7 | 2 | 2 | 20 | 42 |
Unique Cell Count | 44 | 108 | 128 | 226 | 171 | 339 | 164 | 247 | 86 | 40 | 17 | 140 | 180 | 169 | 161 | 186 | 61 | 116 | 19 | 18 | 124 | 150 | ||
Labelled Cell Count | 55 | 114 | 131 | 235 | 194 | 393 | 205 | 308 | 124 | 55 | 19 | 147 | 187 | 179 | 178 | 210 | 61 | 116 | 19 | 18 | 124 | 150 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.4 | 5.6 | – | 6.3 | 6.0 | 5.2 | 4.9 | – | 4.6 | 4.6 | 4.4 | 4.2 | 5.2 | 6.5 | 6.6 | 8.3 | 8.0 | 7.5 | – | 5.8 | 6.2 |
Std Deviation (1e-4) | 0.5 | 0.8 | – | 1.7 | 1.5 | 1.7 | 1.4 | – | 1.8 | 1.3 | 1.9 | 1.5 | 0.8 | 1.3 | 2.2 | 1.8 | 1.7 | 1.6 | – | 1.6 | 2.0 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.4589 | 1.4148 | 1.624 | 1.1988 | 1.8359 | 1.2749 | 0.9824 | 1.1786 | 1.271 | 0.6053 | 0.8184 | 0.9033 | -1.1126 | -0.8227 | -0.6157 | -0.8067 | -0.9176 | -0.758 |
Actin | 0.0072 | 0.0002 | 0.0026 | 0.0065 | 0.0071 | 0.0003 | 0.0603 | 0.0002 | 0.001 | 0.0005 | 0.0178 | 0.0015 | 0.0309 | 0.0001 | 0.0282 | 0.0613 | 0 | 0.0001 |
Bud | 0.0006 | 0.0001 | 0.0009 | 0.0002 | 0.0005 | 0.0002 | 0.0011 | 0.0001 | 0.0005 | 0.0002 | 0.0005 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0001 | 0 | 0.0002 |
Bud Neck | 0.0042 | 0.0002 | 0.0002 | 0.0006 | 0.0055 | 0.0012 | 0.001 | 0.0003 | 0.0063 | 0.0001 | 0.0015 | 0.0051 | 0.0046 | 0.0002 | 0.0009 | 0.0004 | 0.0011 | 0.0019 |
Bud Periphery | 0.0015 | 0.0001 | 0.0004 | 0.0007 | 0.0009 | 0.0002 | 0.0041 | 0.0001 | 0.0011 | 0.0003 | 0.0011 | 0.0004 | 0.0007 | 0 | 0.0002 | 0.0002 | 0 | 0.0002 |
Bud Site | 0.0046 | 0.0002 | 0.0011 | 0.0004 | 0.002 | 0.0002 | 0.004 | 0.0007 | 0.0187 | 0.0002 | 0.001 | 0.0003 | 0.0027 | 0.0001 | 0.0138 | 0.0006 | 0 | 0.0001 |
Cell Periphery | 0.0006 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0008 | 0 | 0.0009 | 0 | 0.0004 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0107 | 0.0036 | 0.0026 | 0.0008 | 0.0025 | 0.0016 | 0.0047 | 0.0094 | 0.0008 | 0.0002 | 0.0008 | 0.0007 | 0.002 | 0.0035 | 0.003 | 0.0012 | 0.0013 | 0.019 |
Cytoplasmic Foci | 0.0112 | 0.0011 | 0.0078 | 0.0107 | 0.0137 | 0.0008 | 0.0313 | 0.0003 | 0.0054 | 0 | 0.0186 | 0.0026 | 0.0157 | 0.001 | 0.0156 | 0.0084 | 0 | 0.0002 |
Eisosomes | 0.0002 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0029 | 0 | 0 | 0 | 0.0001 | 0 | 0.0008 | 0 | 0.0001 | 0.0006 | 0 | 0 |
Endoplasmic Reticulum | 0.0018 | 0.0003 | 0.0006 | 0.0009 | 0.0006 | 0.0006 | 0.008 | 0.0007 | 0.0006 | 0.0001 | 0.0019 | 0.0002 | 0.0005 | 0.0006 | 0.0002 | 0.0005 | 0.0001 | 0.0002 |
Endosome | 0.0164 | 0.0009 | 0.0111 | 0.0065 | 0.0432 | 0.0045 | 0.034 | 0.0015 | 0.0061 | 0.0001 | 0.0249 | 0.0008 | 0.0072 | 0.0007 | 0.0039 | 0.0029 | 0 | 0.0005 |
Golgi | 0.0042 | 0.0001 | 0.0069 | 0.005 | 0.0105 | 0.0012 | 0.0207 | 0.0001 | 0.0018 | 0 | 0.0328 | 0.0008 | 0.002 | 0 | 0.0016 | 0.0031 | 0 | 0.0001 |
Lipid Particles | 0.0335 | 0.0002 | 0.0049 | 0.0161 | 0.0179 | 0.0098 | 0.02 | 0.0002 | 0.0075 | 0 | 0.0362 | 0.0041 | 0.0043 | 0.0004 | 0.0069 | 0.0078 | 0 | 0.0008 |
Mitochondria | 0.0152 | 0.0014 | 0.0029 | 0.0278 | 0.0105 | 0.0045 | 0.0096 | 0.0003 | 0.0015 | 0.0092 | 0.0213 | 0.0004 | 0.0015 | 0.0003 | 0.0003 | 0.0014 | 0.0002 | 0.0001 |
None | 0.0081 | 0.0029 | 0.0025 | 0.0003 | 0.0007 | 0.0006 | 0.0214 | 0.0069 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0024 | 0.0139 | 0.0201 | 0.0063 | 0.0001 | 0.0029 |
Nuclear Periphery | 0.0207 | 0.009 | 0.0041 | 0.0051 | 0.0049 | 0.0047 | 0.014 | 0.0168 | 0.0045 | 0.0014 | 0.0102 | 0.001 | 0.003 | 0.0209 | 0.0022 | 0.009 | 0.0044 | 0.0154 |
Nucleolus | 0.0272 | 0.0109 | 0.0096 | 0.0042 | 0.0353 | 0.0278 | 0.0048 | 0.0089 | 0.0093 | 0.005 | 0.0075 | 0.0147 | 0.0155 | 0.0073 | 0.0053 | 0.0046 | 0.0066 | 0.0122 |
Nucleus | 0.7776 | 0.9351 | 0.9052 | 0.806 | 0.8124 | 0.8913 | 0.7271 | 0.9392 | 0.9248 | 0.9817 | 0.7469 | 0.9539 | 0.8763 | 0.9395 | 0.8876 | 0.8733 | 0.9849 | 0.9313 |
Peroxisomes | 0.0151 | 0.0003 | 0.0026 | 0.015 | 0.011 | 0.0019 | 0.0107 | 0.0001 | 0.0065 | 0.0002 | 0.0246 | 0.0105 | 0.0042 | 0 | 0.0028 | 0.0138 | 0 | 0 |
Punctate Nuclear | 0.0302 | 0.0326 | 0.0269 | 0.0879 | 0.0073 | 0.0472 | 0.0099 | 0.0118 | 0.0006 | 0.0002 | 0.0006 | 0.0016 | 0.0067 | 0.0108 | 0.0065 | 0.0037 | 0.0008 | 0.0139 |
Vacuole | 0.0072 | 0.0007 | 0.0048 | 0.0022 | 0.011 | 0.0006 | 0.0069 | 0.0021 | 0.0015 | 0.0002 | 0.0342 | 0.0012 | 0.0152 | 0.0004 | 0.0004 | 0.0004 | 0.0001 | 0.0005 |
Vacuole Periphery | 0.0022 | 0.0002 | 0.0018 | 0.0028 | 0.0023 | 0.0006 | 0.0027 | 0.0004 | 0.0004 | 0.0002 | 0.0169 | 0.0001 | 0.0029 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.7719 | 7.3316 | 6.2773 | 11.109 | 13.9053 | 11.98 | 6.8427 | 7.5048 | 9.8439 | 17.2399 |
Translational Efficiency | 1.0939 | 1.032 | 0.9506 | 0.7121 | 0.8761 | 1.6122 | 1.0349 | 1.0612 | 0.9657 | 0.7765 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|