Standard name
Human Ortholog
Description Ribonuclease MRP complex subunit; ribonuclease (RNase) MRP cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0 0.08 0.09 0.16 0.23 0.18 0.15 0.12 0.09 0.1 0.13 0.21 0.2 0.2 0.24 0.2 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.08 0 0.09 0 0.56 0.19 0.52 0.47 0.5 0.53 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.83 0.77 0.85 0.81 0.68 0.43 0.55 0.5 0.59 0.55 0.51 0.81 0.72 0.69 0.44 0.39 0.42 0.68 0.7 0.66 0.49 0.42 0.33
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0.05 0.06 0.06 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
Vac/Vac Membrane 0.07 0.19 0.06 0.05 0.16 0.05 0.13 0.07 0.05 0.07 0.07 0.05 0.09 0.15 0.37 0.44 0.41 0.11 0.13 0.16 0.26 0.3 0.31
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 5
Bud 0 0 0 2 0 8 0 2 3 3 7 0 0 0 1 0 0 3 4 0 3 15 11
Bud Neck 0 0 0 0 0 0 2 2 0 3 1 0 1 0 0 0 0 0 0 1 0 6 6
Bud Site 0 0 0 0 0 5 2 6 4 8 15 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 16 7 18 25 45 66 92 59 50 43 51 36 111 120 59 51 39 2 5 7 7 14 21
Endoplasmic Reticulum 0 3 2 0 1 0 0 0 0 0 1 1 0 0 16 8 12 0 0 0 0 3 6
Endosome 3 0 1 11 9 1 23 1 4 2 12 3 5 11 16 14 2 5 5 2 4 25 32
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 3 1 0 2 0 4
Mitochondria 21 23 1 27 7 160 100 199 194 240 271 11 1 4 6 4 4 3 4 3 4 17 16
Nucleus 210 233 194 232 190 121 282 192 248 263 262 221 386 419 129 85 81 213 235 171 107 253 175
Nuclear Periphery 5 0 0 3 0 0 0 0 1 0 3 0 1 0 0 0 0 3 0 2 0 7 9
Nucleolus 23 3 0 2 0 3 2 0 2 0 2 1 5 0 0 0 1 28 22 12 13 33 25
Peroxisomes 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
SpindlePole 0 0 5 2 2 1 20 2 9 3 10 0 1 3 2 2 0 7 7 11 7 28 33
Vac/Vac Membrane 18 59 14 13 46 15 66 28 22 33 36 14 46 94 110 96 79 33 43 40 57 184 163
Unique Cell Count 254 304 228 285 281 284 515 381 417 477 516 273 534 609 294 216 192 313 335 259 218 606 535
Labelled Cell Count 297 328 235 317 300 381 589 491 537 598 671 287 557 652 339 262 218 313 335 259 218 606 535


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 5.4 5.6 5.5 6.0 3.8 4.9 4.1 4.3 4.2 4.3 5.8 6.2 6.0 6.3 6.8 6.5 5.5 5.6 5.7
Std Deviation (1e-4) 0.9 0.7 0.8 1.5 1.6 1.5 1.5 1.4 1.1 0.8 0.8 1.3 1.6 1.8 1.3 1.5 1.4 2.1 1.9 2.1
Intensity Change (Log2) -0.02 0.09 -0.55 -0.2 -0.45 -0.38 -0.43 -0.38 0.04 0.13 0.1 0.17 0.28 0.21 -0.03 -0.02 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 2.8 4.7 3.5 2.7 1.6 0.5 0.9 1.9 4.3 4.1 3.9 4.6 3.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 2.8 0 3.1
Endosome 2.5 0 0 2.9 0 0 0 0 0 0 0 3.2 3.5 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.5 0 0 7.0 0 0 0 0 2.6 0 0 0 0 0
Nucleus -1.1 -4.6 -9.8 -7.9 -8.6 -6.7 -7.8 -8.9 -1.2 -3.8 -4.7 -9.6 -10.0 -9.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1.2 0 0 0 0 0 0 0 0 0 0
Vacuole -0.8 3.6 -0.4 2.7 0.6 -0.5 0.4 0.4 -0.5 1.2 3.6 8.3 9.3 8.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8972 0.7195 0.3944 0.4396 0.5082 0.4515 1.0456 1.6173 1.3302 1.234 1.0088 1.3975 4.3015 4.7387 4.3013 4.4468 3.773 4.1702
Actin 0.0391 0.0002 0.0286 0.0015 0.0084 0.0043 0.0065 0.0001 0.0076 0.0013 0.0072 0 0.0306 0.0014 0.0044 0.0014 0.0065 0.0008
Bud 0.0006 0.0002 0.0005 0.0001 0.0046 0.0005 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0004 0.0002 0.0006 0.0003 0.0002 0.0001
Bud Neck 0.0016 0.001 0.0021 0.0009 0.0729 0.0088 0.001 0.0008 0.0008 0.0011 0.0012 0.0027 0.0038 0.0022 0.0041 0.0014 0.0017 0.004
Bud Periphery 0.0009 0.0003 0.0014 0.0004 0.0073 0.0025 0.0001 0.0001 0.0001 0.0002 0.0002 0.0002 0.0007 0.0002 0.0013 0.0007 0.001 0.0003
Bud Site 0.0018 0.0019 0.0025 0.0004 0.0142 0.001 0.0003 0.0008 0.0005 0.0002 0.0002 0.0003 0.0026 0.0033 0.0025 0.0008 0.0004 0.0004
Cell Periphery 0.0002 0.0002 0.0004 0.0002 0.0007 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0005 0.0001 0.0002 0.0001
Cytoplasm 0.03 0.0154 0.0078 0.017 0.0065 0.0109 0.008 0.0064 0.0043 0.0229 0.001 0.0029 0.0042 0.0123 0.0065 0.0055 0.0163 0.0024
Cytoplasmic Foci 0.0202 0.0021 0.002 0.005 0.0061 0.0026 0.0004 0.0001 0.0001 0.0014 0.0015 0.0013 0.0053 0.0004 0.0044 0.0002 0.0062 0.0003
Eisosomes 0.0003 0 0.0001 0.0001 0.0002 0 0 0.0001 0.0004 0.0001 0.0003 0 0.002 0 0.0001 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0044 0.0025 0.0038 0.0127 0.0014 0.0021 0.0017 0.0003 0.0004 0.0014 0.0008 0.0005 0.0042 0.0019 0.0035 0.001 0.0012 0.0003
Endosome 0.0465 0.0059 0.0131 0.0212 0.008 0.0233 0.0035 0.0003 0.0008 0.0043 0.0074 0.0006 0.0125 0.003 0.0095 0.0033 0.0148 0.0012
Golgi 0.0171 0.0003 0.0025 0.0035 0.0094 0.0134 0.0002 0 0.0001 0.0003 0.0021 0 0.0039 0.0002 0.0022 0.0017 0.0157 0.0001
Lipid Particles 0.0105 0.0033 0.0016 0.0066 0.0052 0.005 0.0022 0.0001 0.0001 0.0006 0.0064 0.0001 0.008 0.0001 0.0025 0.0048 0.0232 0.0003
Mitochondria 0.0065 0.0011 0.0063 0.0571 0.053 0.0034 0.0006 0.0003 0.0007 0.0014 0.004 0.0002 0.0021 0.0005 0.0034 0.0223 0.0144 0.0025
None 0.022 0.0135 0.0111 0.005 0.0018 0.0127 0.0052 0.0128 0.0015 0.0357 0.0037 0.0019 0.0023 0.0023 0.0157 0.0011 0.0068 0.0047
Nuclear Periphery 0.038 0.0357 0.0302 0.0648 0.0159 0.0264 0.0307 0.008 0.0118 0.0439 0.0067 0.0032 0.0204 0.0314 0.005 0.0243 0.0062 0.0053
Nucleolus 0.05 0.0808 0.1188 0.0621 0.094 0.0838 0.0482 0.069 0.079 0.0987 0.0658 0.0508 0.0567 0.0535 0.0744 0.0577 0.027 0.0667
Nucleus 0.6712 0.82 0.7355 0.7265 0.6725 0.7779 0.8797 0.893 0.8837 0.7645 0.8759 0.9187 0.8144 0.8693 0.8363 0.8631 0.8036 0.8997
Peroxisomes 0.0027 0.0003 0.0034 0.0003 0.0082 0.0005 0.0001 0 0.0003 0.0001 0.0032 0 0.013 0.0001 0.0013 0.0006 0.0055 0.0001
Punctate Nuclear 0.0304 0.007 0.0221 0.0103 0.0035 0.0149 0.0086 0.0066 0.0061 0.0194 0.0114 0.0148 0.0094 0.01 0.014 0.0053 0.0381 0.0094
Vacuole 0.0042 0.0066 0.0038 0.0026 0.0044 0.0045 0.0018 0.0008 0.0014 0.0012 0.0003 0.0013 0.0024 0.0062 0.0071 0.0022 0.0069 0.001
Vacuole Periphery 0.0019 0.0015 0.0021 0.0016 0.0021 0.0012 0.0009 0.0002 0.0003 0.0011 0.0005 0.0002 0.0008 0.0014 0.0006 0.0021 0.0039 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.3171 8.2611 9.8082 8.2194 13.1798 3.6945 7.0999 10.7114 8.2398 11.1036
Translational Efficiency 1.5961 1.5815 1.2469 1.9149 1.1717 3.0532 1.8665 0.8535 1.2314 1.3706

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Ribonuclease MRP complex subunit; ribonuclease (RNase) MRP cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
Localization
Cell Percentages nucleus (90%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Snm1

Snm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Snm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available