Standard name | |
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Human Ortholog | |
Description | Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 0 | 2 | 4 | 3 | 5 | 2 | 5 | 0 | 2 | 3 | 1 | 4 | 3 | 4 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 12 | 23 |
Bud | 13 | 0 | 7 | 11 | 48 | 40 | 42 | 66 | 40 | 36 | 24 | 31 | 5 | 5 | 12 | 5 | 0 | 0 | 100 | 13 | 30 | 9 | 6 | 13 |
Bud Neck | 59 | 1 | 20 | 21 | 76 | 38 | 71 | 97 | 42 | 49 | 28 | 32 | 13 | 19 | 27 | 20 | 7 | 11 | 131 | 8 | 33 | 12 | 12 | 22 |
Bud Site | 41 | 2 | 10 | 24 | 50 | 35 | 39 | 28 | 22 | 17 | 8 | 23 | 10 | 21 | 19 | 9 | 1 | 4 | – | – | – | – | – | – |
Cell Periphery | 28 | 0 | 0 | 6 | 6 | 9 | 7 | 6 | 12 | 11 | 12 | 22 | 6 | 6 | 14 | 12 | 3 | 7 | 1 | 0 | 0 | 0 | 2 | 9 |
Cytoplasm | 158 | 11 | 50 | 71 | 107 | 96 | 217 | 254 | 145 | 207 | 149 | 180 | 35 | 49 | 54 | 32 | 24 | 43 | 102 | 23 | 29 | 38 | 124 | 98 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 1 | 1 | 3 |
Endosome | 6 | 0 | 2 | 1 | 6 | 4 | 5 | 5 | 0 | 0 | 0 | 0 | 2 | 6 | 2 | 1 | 1 | 3 | 3 | 0 | 0 | 0 | 1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 1 | 1 | 8 | 20 | 15 | 27 | 43 | 79 | 67 | 103 | 67 | 103 | 1 | 2 | 4 | 8 | 0 | 5 | 1 | 0 | 1 | 2 | 1 | 5 |
Nucleus | 19 | 0 | 6 | 13 | 18 | 25 | 24 | 39 | 12 | 20 | 9 | 6 | 1 | 7 | 11 | 0 | 1 | 2 | 0 | 0 | 2 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 42 | 1 | 12 | 5 | 27 | 26 | 33 | 70 | 28 | 40 | 16 | 21 | 8 | 13 | 12 | 15 | 5 | 12 | 3 | 0 | 3 | 0 | 0 | 0 |
Peroxisomes | 3 | 1 | 2 | 3 | 13 | 7 | 4 | 4 | 4 | 6 | 2 | 2 | 8 | 8 | 5 | 2 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 36 | 0 | 4 | 15 | 45 | 43 | 65 | 64 | 29 | 31 | 15 | 25 | 9 | 16 | 13 | 6 | 3 | 4 | 21 | 1 | 7 | 0 | 0 | 0 |
Vac/Vac Membrane | 7 | 1 | 0 | 1 | 3 | 4 | 2 | 10 | 2 | 1 | 5 | 5 | 1 | 0 | 0 | 5 | 1 | 3 | 7 | 1 | 1 | 1 | 3 | 5 |
Unique Cell Count | 322 | 18 | 96 | 150 | 309 | 246 | 421 | 534 | 275 | 375 | 250 | 316 | 75 | 120 | 132 | 83 | 37 | 81 | 383 | 54 | 115 | 69 | 172 | 192 |
Labelled Cell Count | 418 | 19 | 123 | 195 | 417 | 359 | 556 | 730 | 404 | 523 | 339 | 451 | 104 | 155 | 177 | 119 | 46 | 100 | 383 | 54 | 115 | 69 | 172 | 192 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.4 | 6.1 | 5.2 | 5.1 | 5.5 | 5.0 | 4.7 | 4.7 | 4.2 | 4.1 | 4.0 | 3.7 | 8.0 | 8.1 | 7.2 | 12.1 | 11.4 | 14.6 | 6.8 | 7.2 | 6.6 |
Std Deviation (1e-4) | 2.9 | 1.4 | 1.3 | 1.2 | 1.4 | 1.3 | 1.4 | 1.5 | 1.1 | 1.4 | 1.3 | 1.3 | 2.4 | 2.4 | 2.2 | 3.0 | 4.7 | 5.8 | 1.2 | 7.5 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.08 | -0.05 | -0.15 | -0.14 | -0.3 | -0.34 | -0.37 | -0.47 | 0.63 | 0.66 | 0.49 | 1.22 | 1.14 | 1.5 | 0.39 | 0.47 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 2.1 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | -0.2 | -1.0 | 0.5 | -0.3 | 0 | 0 |
Bud Neck | -1.4 | 0.8 | -1.2 | 0 | 0 | 0 | 0 | 0 | 0 | -0.6 | -0.9 | -0.1 | 0.5 | -0.2 | -1.3 |
Bud Site | 1.2 | 1.4 | 0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0.6 | 1.5 | 0.9 | 0.1 | 0 | -1.3 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 3.9 | 0 | 0 |
Cytoplasm | -0.7 | -3.1 | -2.2 | -0.1 | -0.8 | 0.1 | 0.5 | 1.3 | 0.8 | -0.7 | -1.6 | -1.7 | -1.8 | 1.3 | 0.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.2 | -1.3 | 0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.8 | 0.3 | 0 | -0.5 |
Nucleus | 0.7 | -0.2 | 1.1 | -0.2 | 0.4 | 0 | -0.4 | 0 | 0 | 0 | -0.1 | 0.6 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -2.8 | -1.1 | -0.5 | 0 | 0 | 0 | 0 | 0 | 0 | -0.4 | -0.4 | -0.8 | 1.0 | 0 | 0.4 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1.7 | 2.7 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 2.3 | 1.6 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.3191 | 5.053 | 5.2925 | 5.0205 | 4.2327 | 4.4829 | 4.5631 | 6.928 | 8.1346 | 6.9925 | 6.1684 | 6.6168 | 2.4267 | 4.7324 | 5.2301 | 5.104 | 4.9833 | 3.6826 |
Actin | 0.0053 | 0.0008 | 0.001 | 0.0001 | 0.0002 | 0.0103 | 0.0731 | 0.0173 | 0.032 | 0.0529 | 0.0274 | 0.015 | 0.0102 | 0.0015 | 0.0067 | 0.0116 | 0.0002 | 0.0063 |
Bud | 0.0289 | 0.0257 | 0.0083 | 0.0002 | 0.0019 | 0.0207 | 0.017 | 0.033 | 0.0092 | 0.0006 | 0.0021 | 0.0228 | 0.0238 | 0.0407 | 0.0075 | 0.0007 | 0.0012 | 0.0381 |
Bud Neck | 0.0457 | 0.0409 | 0.6618 | 0.9053 | 0.9193 | 0.4683 | 0.0382 | 0.0244 | 0.5757 | 0.8695 | 0.4883 | 0.4191 | 0.0571 | 0.0287 | 0.6881 | 0.953 | 0.9163 | 0.4249 |
Bud Periphery | 0.0182 | 0.0291 | 0.0087 | 0.0002 | 0.0023 | 0.0207 | 0.0119 | 0.0262 | 0.0052 | 0.0008 | 0.0038 | 0.033 | 0.0176 | 0.0184 | 0.0074 | 0.0008 | 0.0017 | 0.0411 |
Bud Site | 0.4261 | 0.7021 | 0.2879 | 0.0048 | 0.0028 | 0.0433 | 0.2494 | 0.6486 | 0.2874 | 0.0025 | 0.018 | 0.0226 | 0.4617 | 0.6388 | 0.1984 | 0.0037 | 0.0255 | 0.0278 |
Cell Periphery | 0.0087 | 0.0055 | 0.0033 | 0.0009 | 0.0014 | 0.0052 | 0.0068 | 0.0089 | 0.0042 | 0.001 | 0.0017 | 0.002 | 0.0067 | 0.0083 | 0.0031 | 0.0006 | 0.0014 | 0.0053 |
Cytoplasm | 0.2281 | 0.0855 | 0.0003 | 0.0439 | 0.0004 | 0.3054 | 0.2412 | 0.1632 | 0.0098 | 0.0011 | 0.0197 | 0.3886 | 0.1697 | 0.073 | 0.0006 | 0.003 | 0.0001 | 0.3318 |
Cytoplasmic Foci | 0.1161 | 0.027 | 0.0076 | 0.0035 | 0.0131 | 0.074 | 0.1224 | 0.0316 | 0.0406 | 0.0279 | 0.0651 | 0.0323 | 0.0892 | 0.0436 | 0.0239 | 0.0023 | 0.0246 | 0.0526 |
Eisosomes | 0.0004 | 0 | 0 | 0 | 0 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0003 | 0.0011 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0054 | 0.0003 | 0 | 0.0003 | 0 | 0.0012 | 0.0149 | 0.0008 | 0.0001 | 0.0012 | 0.0054 | 0.0013 | 0.0048 | 0.0006 | 0.0001 | 0.0004 | 0 | 0.0017 |
Endosome | 0.0013 | 0.0008 | 0.0001 | 0.0012 | 0.0005 | 0.0015 | 0.0634 | 0.001 | 0.0025 | 0.008 | 0.1115 | 0.0038 | 0.0148 | 0.0011 | 0.0016 | 0.0013 | 0.0002 | 0.0043 |
Golgi | 0.0009 | 0.0002 | 0.0002 | 0.0003 | 0.0006 | 0.0013 | 0.0319 | 0.0011 | 0.0039 | 0.015 | 0.0689 | 0.0033 | 0.0049 | 0.0003 | 0.0016 | 0.0006 | 0.0003 | 0.0015 |
Lipid Particles | 0.0064 | 0.0005 | 0.0005 | 0.0003 | 0.0038 | 0.0028 | 0.0439 | 0.0009 | 0.0044 | 0.0032 | 0.0244 | 0.0068 | 0.0115 | 0.0007 | 0.0065 | 0.0002 | 0.0152 | 0.0042 |
Mitochondria | 0.0016 | 0.0034 | 0.0002 | 0.0001 | 0.0006 | 0.0005 | 0.0073 | 0.0012 | 0.0042 | 0.0008 | 0.0513 | 0.0045 | 0.0127 | 0.0014 | 0.0066 | 0.0003 | 0.001 | 0.0049 |
None | 0.071 | 0.0024 | 0.0001 | 0.0002 | 0 | 0.0088 | 0.0112 | 0.002 | 0.0003 | 0.0002 | 0.0087 | 0.0157 | 0.0369 | 0.0162 | 0.0004 | 0.0013 | 0.0001 | 0.0241 |
Nuclear Periphery | 0.0005 | 0.0014 | 0 | 0 | 0 | 0.0003 | 0.0207 | 0.0011 | 0.0001 | 0.0002 | 0.0088 | 0.0003 | 0.0017 | 0.0007 | 0.0003 | 0.0005 | 0 | 0.0007 |
Nucleolus | 0.013 | 0.0165 | 0.0122 | 0.034 | 0.0423 | 0.0065 | 0.0045 | 0.0109 | 0.0094 | 0.0037 | 0.0113 | 0.0214 | 0.0272 | 0.0307 | 0.0219 | 0.0144 | 0.007 | 0.0047 |
Nucleus | 0.0026 | 0.0378 | 0.0033 | 0.0029 | 0.0042 | 0.0032 | 0.0041 | 0.0069 | 0.0022 | 0.0008 | 0.0065 | 0.0013 | 0.0044 | 0.037 | 0.0033 | 0.0025 | 0.0018 | 0.0048 |
Peroxisomes | 0.0056 | 0.0007 | 0.0004 | 0.0001 | 0.0037 | 0.003 | 0.0141 | 0.0011 | 0.0049 | 0.0063 | 0.0111 | 0.0026 | 0.0181 | 0.0031 | 0.0139 | 0.0007 | 0.0017 | 0.0026 |
Punctate Nuclear | 0.0088 | 0.0092 | 0.0024 | 0.0006 | 0.0011 | 0.0198 | 0.0112 | 0.0029 | 0.001 | 0.0033 | 0.0488 | 0.0015 | 0.0155 | 0.0092 | 0.0061 | 0.002 | 0.0009 | 0.0076 |
Vacuole | 0.0052 | 0.0085 | 0.0016 | 0.0009 | 0.0015 | 0.003 | 0.0089 | 0.0159 | 0.0023 | 0.0005 | 0.0113 | 0.0018 | 0.0104 | 0.043 | 0.0018 | 0.0002 | 0.0006 | 0.0101 |
Vacuole Periphery | 0.0003 | 0.0016 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0033 | 0.0008 | 0.0002 | 0.0001 | 0.0046 | 0.0004 | 0.001 | 0.0026 | 0.0002 | 0 | 0.0001 | 0.0011 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 78.5158 | 28.7657 | 19.6378 | 40.0398 | 71.5515 | 80.402 | 29.0123 | 21.986 | 32.3802 | 77.2415 |
Translational Efficiency | 1.5588 | 1.5435 | 0.9404 | 1.0186 | 1.2272 | 1.569 | 1.1018 | 0.8093 | 0.8441 | 1.0614 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
789 | 1212 | 170 | 1151 | 741 | 1543 | 44 | 3089 | 1530 | 2755 | 214 | 4240 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 732.53 | 863.14 | 1285.60 | 1152.63 | 775.00 | 890.41 | 1206.91 | 1204.23 | 753.10 | 878.41 | 1269.42 | 1190.22 |
Standard Deviation | 319.29 | 164.61 | 169.73 | 199.21 | 126.06 | 134.30 | 144.93 | 228.59 | 246.41 | 149.02 | 167.97 | 222.19 |
Intensity Change Log 2 | — | 0.236707 | 0.811482 | 0.653970 | — | 0.200273 | 0.639050 | 0.635843 | — | 0.218092 | 0.725413 | 0.644679 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000656 | 0.000832 | 0.001639 | 0.002163 | 0.000809 | 0.000730 | 0.000584 | 0.000624 | 0.000730 | 0.000775 | 0.001422 | 0.001042 |
Bud Neck | 0.151015 | 0.157709 | 0.121681 | 0.147095 | 0.195990* | 0.177526 | 0.102023 | 0.186798* | 0.172797 | 0.168808 | 0.117639 | 0.176020 |
Bud Site | 0.378841* | 0.475782* | 0.442893* | 0.592914* | 0.493805* | 0.508370* | 0.392949* | 0.603022* | 0.434519* | 0.494034* | 0.432624* | 0.600278* |
Cell Periphery | 0.006458 | 0.004747 | 0.000897 | 0.002705 | 0.004644 | 0.005115 | 0.000466 | 0.005655 | 0.005579 | 0.004953 | 0.000808 | 0.004854 |
Cytoplasm | 0.265338* | 0.179613* | 0.160169* | 0.112254* | 0.153083* | 0.176484* | 0.125578* | 0.126064* | 0.210971* | 0.177861* | 0.153057* | 0.122315* |
Cytoplasmic Foci | 0.090590 | 0.088409 | 0.113032 | 0.052544 | 0.071877 | 0.070717 | 0.154295 | 0.031633 | 0.081527 | 0.078500 | 0.121516 | 0.037309 |
Eisosomes | 0.001614 | 0.001485 | 0.001189 | 0.001263 | 0.000994 | 0.001466 | 0.001896 | 0.003497 | 0.001314 | 0.001474 | 0.001334 | 0.002890 |
Endoplasmic Reticulum | 0.000742 | 0.001524 | 0.001828 | 0.000328 | 0.000676 | 0.000436 | 0.001050 | 0.000150 | 0.000710 | 0.000914 | 0.001668 | 0.000199 |
Endosome | 0.008266 | 0.009803 | 0.004282 | 0.004612 | 0.009643 | 0.008479 | 0.008142 | 0.001091 | 0.008933 | 0.009061 | 0.005076 | 0.002047 |
Golgi | 0.002573 | 0.003531 | 0.000515 | 0.003820 | 0.002505 | 0.004223 | 0.000925 | 0.000841 | 0.002540 | 0.003919 | 0.000599 | 0.001649 |
Lipid Particles | 0.001190 | 0.001013 | 0.001846 | 0.000596 | 0.000747 | 0.001415 | 0.009345 | 0.000080 | 0.000976 | 0.001238 | 0.003388 | 0.000220 |
Mitochondria | 0.002652 | 0.004217 | 0.000344 | 0.005670 | 0.003171 | 0.001177 | 0.000421 | 0.008181 | 0.002904 | 0.002514 | 0.000360 | 0.007499 |
Mitotic Spindle | 0.001821 | 0.004983 | 0.005010 | 0.013022 | 0.002965 | 0.004681 | 0.013318 | 0.006044 | 0.002375 | 0.004814 | 0.006718 | 0.007938 |
None | 0.017765 | 0.002888 | 0.000408 | 0.000401 | 0.003342 | 0.001029 | 0.000103 | 0.000790 | 0.010780 | 0.001847 | 0.000345 | 0.000684 |
Nuclear Periphery | 0.000069 | 0.000067 | 0.000264 | 0.000100 | 0.000059 | 0.000038 | 0.000244 | 0.000044 | 0.000064 | 0.000051 | 0.000260 | 0.000059 |
Nuclear Periphery Foci | 0.002318 | 0.001406 | 0.007323 | 0.000755 | 0.001242 | 0.001020 | 0.006416 | 0.000385 | 0.001797 | 0.001190 | 0.007137 | 0.000485 |
Nucleolus | 0.004715 | 0.008106 | 0.009431 | 0.000926 | 0.005313 | 0.000859 | 0.001979 | 0.002126 | 0.005005 | 0.004047 | 0.007899 | 0.001800 |
Nucleus | 0.023182 | 0.010387 | 0.022116 | 0.003495 | 0.007424 | 0.005565 | 0.010824 | 0.002898 | 0.015550 | 0.007686 | 0.019794 | 0.003060 |
Peroxisomes | 0.012979 | 0.018316 | 0.015226 | 0.008821 | 0.017787 | 0.007438 | 0.029313 | 0.010550 | 0.015308 | 0.012223 | 0.018122 | 0.010080 |
Vacuole | 0.026068 | 0.023262 | 0.089689 | 0.044188 | 0.021820 | 0.022199 | 0.139926 | 0.008809 | 0.024011 | 0.022667 | 0.100018 | 0.018413 |
Vacuole Periphery | 0.001149 | 0.001919 | 0.000218 | 0.002330 | 0.002104 | 0.001034 | 0.000203 | 0.000720 | 0.001611 | 0.001423 | 0.000214 | 0.001157 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.21 | -3.03 | -3.60 | -3.15 | -0.51 | 0.28 | 0.12 | 0.21 | -0.09 | 0.05 | -0.32 | -2.75 | -2.25 | -2.06 | 1.46 |
Bud Neck | -0.68 | 4.77 | 2.36 | 3.77 | -3.46 | 1.79 | 5.72 | 1.31 | -0.92 | -5.58 | 0.56 | 7.94 | 0.64 | 0.05 | -8.64 |
Bud Site | -6.41 | 1.67 | -10.20 | -4.42 | -7.32 | -0.95 | 2.84 | -7.87 | -9.16 | -4.84 | -5.62 | 4.09 | -14.97 | -11.21 | -10.36 |
Cell Periphery | 1.33 | 5.04 | 3.30 | 2.65 | -3.28 | -0.56 | 6.98 | -1.42 | -0.74 | -15.32 | 0.80 | 7.39 | 1.13 | 0.31 | -13.15 |
Cytoplasm | 6.29 | 2.52 | 8.61 | 2.66 | 2.58 | -2.09 | -0.23 | 1.87 | 5.30 | 0.81 | 3.66 | 0.71 | 8.74 | 6.21 | 3.28 |
Cytoplasmic Foci | 0.23 | -1.30 | 5.25 | 5.64 | 4.16 | 0.09 | -3.02 | 7.73 | 10.27 | 4.77 | 0.53 | -3.00 | 10.71 | 12.65 | 7.16 |
Eisosomes | 0.31 | 1.92 | 1.39 | 1.57 | -1.11 | -1.92 | -1.36 | -6.46 | -4.85 | -3.07 | -0.67 | 1.13 | -4.63 | -4.46 | -5.70 |
Endoplasmic Reticulum | -1.02 | -3.92 | 3.74 | 1.58 | 4.71 | 1.30 | -1.48 | 3.43 | 2.77 | 3.34 | -0.58 | -4.11 | 5.68 | 2.12 | 5.35 |
Endosome | -1.10 | 2.40 | 3.50 | 4.35 | 1.13 | 0.83 | 0.45 | 7.43 | 7.91 | 2.99 | -0.06 | 2.64 | 9.36 | 9.85 | 5.04 |
Golgi | -2.04 | 6.95 | -0.66 | 0.19 | -2.54 | -2.61 | 3.60 | 7.00 | 5.44 | -0.04 | -3.25 | 9.24 | 2.96 | 4.97 | -2.86 |
Lipid Particles | 0.43 | -3.79 | 1.96 | 0.69 | 4.71 | -1.74 | -1.13 | 5.30 | 3.56 | 1.23 | -0.93 | -2.18 | 6.28 | 3.55 | 2.69 |
Mitochondria | -0.99 | 1.81 | -1.51 | -0.55 | -4.53 | 3.06 | 4.52 | -5.15 | -9.00 | -10.38 | 0.44 | 3.56 | -4.75 | -6.15 | -11.28 |
Mitotic Spindle | -2.56 | -1.93 | -5.66 | -3.92 | -0.63 | -1.57 | -0.94 | -4.65 | -1.78 | 0.53 | -3.01 | -2.07 | -7.83 | -3.93 | 0.58 |
None | 5.41 | 6.53 | 6.54 | 3.40 | -0.00 | 2.02 | 2.93 | 2.31 | 0.82 | -11.61 | 5.86 | 7.02 | 6.81 | 3.22 | -2.91 |
Nuclear Periphery | -0.04 | -5.81 | -3.91 | -3.55 | 4.84 | 1.64 | -2.26 | 0.76 | -1.73 | 2.36 | 1.13 | -6.24 | -1.32 | -2.77 | 6.08 |
Nuclear Periphery Foci | 2.11 | -5.35 | 3.36 | 1.73 | 6.66 | 1.04 | -3.54 | 5.62 | 4.52 | 4.02 | 2.49 | -6.59 | 5.53 | 4.76 | 7.84 |
Nucleolus | -1.74 | -0.46 | 3.60 | 5.04 | 1.36 | 3.50 | 2.46 | 2.48 | -4.47 | -0.57 | 1.02 | -0.22 | 3.87 | 3.41 | 1.14 |
Nucleus | 4.09 | -2.11 | 6.27 | 3.05 | 6.30 | 1.14 | -1.37 | 3.60 | 2.45 | 2.56 | 4.45 | -3.58 | 7.52 | 4.43 | 6.98 |
Peroxisomes | -2.31 | 0.64 | 2.71 | 5.39 | 1.11 | 4.40 | -0.79 | 3.18 | -2.67 | 1.62 | 1.89 | 0.43 | 3.75 | 2.33 | 1.50 |
Vacuole | 0.97 | -7.97 | -8.54 | -9.35 | 5.09 | -0.16 | -4.50 | 5.51 | 6.64 | 4.83 | 0.70 | -9.27 | -1.80 | -2.67 | 9.02 |
Vacuole Periphery | -2.24 | 5.17 | -1.37 | -0.15 | -2.74 | 0.97 | 1.86 | 1.32 | 0.69 | -5.62 | 0.32 | 2.64 | 0.97 | 1.03 | -4.29 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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