ORF
Human Ortholog
Description Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.64 0.66 0.7 0.48 0.62 0.43 0.44 0.44 0.41 0.46 0.25 0.44 0.88 0.94 0.96 – 0.84 0.89 0.22 0.33 0.22 0.19 0.24 0.16
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.1 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0.05 0.06 0.06 0.05 0.06 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.47 0.41 0.32 0.58 0.41 0.67 0.66 0.62 0.71 0.73 0.86 0.74 0.07 0.05 0 – 0.07 0 0.56 0.38 0.52 0.54 0.49 0.49
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0.05 0.06 0.05 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0.06 0.06 0
Vac/Vac Membrane 0 0.07 0.07 0 0.05 0.06 0 0 0 0 0 0 0.09 0.05 0 – 0.09 0.06 0.05 0.12 0.05 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 6 2 4 4 8 25
Bud 0 0 7 7 4 8 8 16 10 21 19 23 0 2 0 – 1 3 1 2 0 1 3 8
Bud Neck 0 0 1 1 0 1 0 7 0 3 2 1 0 0 0 – 3 0 1 0 0 0 1 14
Bud Site 0 0 0 1 0 4 3 4 8 8 1 7 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 0 0 2 1 1 2 1 2 0 1 2 0 0 0 – 2 4 1 0 2 0 3 4
Cytoplasm 195 50 125 78 152 119 224 241 191 238 111 268 177 434 427 – 218 154 80 36 47 25 75 63
Endoplasmic Reticulum 8 1 0 1 1 1 0 0 1 0 3 0 1 1 2 – 26 8 8 0 4 0 3 5
Endosome 0 1 3 1 8 1 0 2 0 0 0 0 3 4 2 – 7 2 17 6 11 6 18 26
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 – 2 0 1 1 1 0 1 1
Mitochondria 145 31 58 94 100 186 337 346 331 376 379 457 14 25 18 – 17 7 208 42 110 71 153 192
Nucleus 0 0 1 0 1 3 4 8 2 3 2 7 0 0 1 – 2 1 0 0 0 0 0 0
Nuclear Periphery 1 0 0 1 0 1 1 0 2 1 1 0 0 0 1 – 0 0 0 1 1 0 0 2
Nucleolus 0 0 1 0 1 0 0 3 1 1 0 2 0 0 2 – 0 0 0 0 0 1 0 1
Peroxisomes 0 0 0 0 0 1 1 1 1 1 1 1 0 0 0 – 0 0 3 0 2 0 0 3
SpindlePole 2 0 1 5 7 14 31 26 17 23 10 28 1 0 1 – 5 4 8 2 8 7 17 13
Vac/Vac Membrane 9 5 12 6 13 17 11 8 6 10 7 7 19 21 5 – 24 10 19 13 10 5 14 23
Unique Cell Count 306 76 179 162 244 277 511 554 463 516 442 616 202 462 443 258 174 370 111 213 133 312 397
Labelled Cell Count 361 88 209 197 289 357 622 663 572 685 537 803 215 487 460 307 193 370 111 213 133 312 397


Mitochondria, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 3.7 3.7 3.6 3.7 4.0 2.6 3.0 2.9 2.6 2.6 2.6 4.5 4.2 4.1 4.4 5.0 5.3 4.2 4.3 4.8
Std Deviation (1e-4) 0.4 1.1 1.2 1.0 1.3 6.4 0.9 1.1 1.1 0.8 0.5 1.2 1.6 1.1 0.9 1.4 1.8 1.7 1.7 1.9 1.8
Intensity Change (Log2) – – – -0.06 -0.04 0.1 -0.52 -0.33 -0.39 -0.54 -0.51 -0.54 0.27 0.16 0.12 0.23 0.43 0.51 0.16 0.19 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 1.0 -0.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.486 -0.2294 -0.2943 -0.6879 -0.2395 -0.3158 0.5555 0.7915 0.5953 0.7756 0.7432 0.8764 0.5333 1.501 0.6686 1.0638 0.9683 0.7284
Actin 0.0222 0.0174 0.0249 0.0148 0.0171 0.0138 0.0205 0.0148 0.0236 0.0439 0.0047 0.0062 0.0315 0.0113 0.0127 0.0222 0.0441 0.0081
Bud 0.0011 0.0046 0.002 0.003 0.0045 0.0017 0.001 0.0129 0.0012 0.0057 0.0005 0.0006 0.0037 0.0028 0.0013 0.0058 0.0027 0.0005
Bud Neck 0.0119 0.002 0.0018 0.0006 0.0009 0.017 0.0016 0.0003 0.0014 0.0023 0.0024 0.0105 0.0087 0.0009 0.0008 0.0033 0.003 0.0039
Bud Periphery 0.0023 0.0067 0.0038 0.0107 0.0119 0.006 0.0026 0.0026 0.0029 0.0263 0.0012 0.0011 0.0062 0.0033 0.0033 0.008 0.007 0.0009
Bud Site 0.0103 0.038 0.0119 0.006 0.0043 0.0023 0.0047 0.0068 0.0085 0.0022 0.0012 0.0017 0.0196 0.0307 0.0045 0.005 0.0021 0.001
Cell Periphery 0.0015 0.0052 0.0011 0.0085 0.0012 0.0007 0.001 0.0011 0.0013 0.0005 0.0009 0.0005 0.0026 0.002 0.0019 0.0013 0.0005 0.0009
Cytoplasm 0.1337 0.2561 0.1959 0.2448 0.2446 0.2009 0.1376 0.1335 0.214 0.1744 0.1555 0.2124 0.1471 0.1716 0.2233 0.2215 0.1328 0.2252
Cytoplasmic Foci 0.106 0.0994 0.1035 0.1066 0.0686 0.1738 0.107 0.0639 0.0764 0.0517 0.1761 0.1028 0.1676 0.0895 0.1307 0.1561 0.1468 0.1589
Eisosomes 0.0008 0.0011 0.001 0.001 0.0004 0.0003 0.0011 0.0014 0.0007 0.0005 0.0007 0.0002 0.0014 0.001 0.0009 0.0008 0.0007 0.0003
Endoplasmic Reticulum 0.0276 0.0194 0.0135 0.0146 0.0074 0.0108 0.013 0.0071 0.0181 0.0262 0.0106 0.0052 0.0078 0.005 0.0144 0.0105 0.0073 0.0154
Endosome 0.1052 0.0773 0.0733 0.0582 0.0777 0.0894 0.0505 0.0549 0.0387 0.0491 0.0452 0.0769 0.0717 0.0341 0.0858 0.0611 0.101 0.0988
Golgi 0.0315 0.0182 0.0154 0.0154 0.0288 0.0211 0.0174 0.0042 0.009 0.032 0.0095 0.0119 0.0206 0.0215 0.022 0.0156 0.0378 0.0201
Lipid Particles 0.0421 0.0127 0.0242 0.02 0.0239 0.0306 0.0391 0.0146 0.008 0.0064 0.0555 0.0237 0.0425 0.0213 0.015 0.0212 0.0124 0.0142
Mitochondria 0.0466 0.0664 0.0905 0.0799 0.1393 0.0779 0.0429 0.0136 0.0595 0.0965 0.0164 0.0456 0.08 0.076 0.1012 0.0997 0.1287 0.0897
None 0.3657 0.3272 0.3646 0.3143 0.3008 0.254 0.4901 0.6316 0.502 0.449 0.4322 0.4577 0.3054 0.5035 0.3335 0.32 0.28 0.3014
Nuclear Periphery 0.0153 0.0051 0.0076 0.0097 0.0113 0.0127 0.0048 0.0019 0.0044 0.0071 0.0017 0.0023 0.0032 0.0014 0.0067 0.0029 0.0069 0.0035
Nucleolus 0.0095 0.0014 0.002 0.0009 0.0009 0.0016 0.0016 0.0007 0.0011 0.0004 0.0013 0.0008 0.0018 0.0009 0.001 0.0008 0.0005 0.001
Nucleus 0.0124 0.0119 0.0142 0.0081 0.0363 0.0159 0.0132 0.0025 0.0042 0.0024 0.0013 0.0053 0.0055 0.0022 0.0059 0.0036 0.0024 0.0152
Peroxisomes 0.0257 0.0066 0.0274 0.0636 0.0054 0.0418 0.0317 0.0042 0.0154 0.0109 0.0675 0.0209 0.0517 0.0139 0.0212 0.0295 0.0724 0.0233
Punctate Nuclear 0.0106 0.0078 0.0113 0.0036 0.003 0.0109 0.0069 0.0012 0.0034 0.0012 0.0098 0.0024 0.0105 0.0021 0.0047 0.0028 0.0046 0.0088
Vacuole 0.0133 0.0128 0.0079 0.0111 0.0078 0.0133 0.0097 0.0239 0.0048 0.0072 0.005 0.0091 0.009 0.0041 0.0067 0.0061 0.0039 0.0067
Vacuole Periphery 0.0049 0.0026 0.0024 0.0045 0.0036 0.0035 0.0021 0.0022 0.0014 0.0041 0.0009 0.0023 0.0018 0.0012 0.0024 0.0023 0.0022 0.0024

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.499 10.1898 7.5613 9.9336 8.3748 4.557 11.6767 9.9671 10.4055 10.1811
Translational Efficiency 2.1541 1.7299 2.1229 1.3298 1.3831 3.4835 1.421 1.2473 1.1334 1.4623

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2234 1880 194 1430 484 2321 939 746 2718 4201 1133 2176

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 593.81 695.19 871.75 833.56 720.24 695.18 886.38 820.55 616.32 695.18 883.87 829.10
Standard Deviation 139.77 91.97 86.74 111.78 77.21 90.59 94.95 104.74 139.49 91.21 93.75 109.59
Intensity Change Log 2 — 0.227406 0.553913 0.489285 — -0.051091 0.299448 0.188114 — 0.081449 0.420022 0.332035

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000176 0.000963 0.000615 0.004882 0.000157 0.000825 0.000227 0.001097 0.000172 0.000887 0.000293 0.003584
Bud Neck 0.046896 0.065409 0.002468 0.035891 0.021618 0.065308 0.002868 0.018578 0.042395 0.065353 0.002799 0.029956
Bud Site 0.014139 0.026013 0.007309 0.048437 0.004710 0.021407 0.003625 0.022087 0.012460 0.023468 0.004255 0.039404
Cell Periphery 0.000694 0.000161 0.000156 0.000321 0.000148 0.000142 0.000144 0.000276 0.000597 0.000150 0.000146 0.000305
Cytoplasm 0.321691* 0.126511* 0.217871* 0.234695* 0.376042* 0.140076* 0.411445* 0.226814* 0.331370* 0.134005* 0.378300* 0.231993*
Cytoplasmic Foci 0.205134* 0.261928* 0.011521 0.037198 0.260184* 0.285054* 0.025382 0.062218 0.214937* 0.274705* 0.023009 0.045776
Eisosomes 0.000324 0.000174 0.000061 0.000116 0.000121 0.000176 0.000052 0.000121 0.000288 0.000176 0.000054 0.000118
Endoplasmic Reticulum 0.004717 0.001651 0.008692 0.012897 0.001005 0.001049 0.003285 0.005594 0.004056 0.001318 0.004211 0.010393
Endosome 0.012388 0.049131 0.002929 0.008286 0.015823 0.035864 0.004328 0.022022 0.013000 0.041801 0.004088 0.012995
Golgi 0.004876 0.031589 0.000105 0.003097 0.004229 0.030231 0.000125 0.005549 0.004761 0.030839 0.000122 0.003937
Lipid Particles 0.024863 0.013532 0.002485 0.004016 0.009126 0.014135 0.002424 0.008603 0.022061 0.013865 0.002435 0.005589
Mitochondria 0.031548 0.156179* 0.000531 0.009970 0.011988 0.150007* 0.000434 0.010063 0.028065 0.152769* 0.000450 0.010002
Mitotic Spindle 0.000657 0.020673 0.001907 0.057894 0.001570 0.018714 0.001451 0.023840 0.000819 0.019591 0.001529 0.046219
None 0.019377 0.002113 0.003536 0.005203 0.005840 0.002254 0.004285 0.007217 0.016967 0.002191 0.004157 0.005893
Nuclear Periphery 0.000939 0.000873 0.001821 0.002907 0.000664 0.000601 0.001784 0.003536 0.000890 0.000723 0.001790 0.003123
Nuclear Periphery Foci 0.000382 0.000463 0.002355 0.002264 0.000540 0.000357 0.001988 0.004045 0.000411 0.000404 0.002051 0.002875
Nucleolus 0.003588 0.004804 0.000708 0.002868 0.004494 0.003448 0.000912 0.006985 0.003749 0.004055 0.000877 0.004279
Nucleus 0.253257* 0.083100 0.675569* 0.399637* 0.235701* 0.097407 0.463255* 0.440067* 0.250130* 0.091005 0.499609* 0.413498*
Peroxisomes 0.008759 0.075636 0.000681 0.017161 0.011016 0.072261 0.000411 0.010090 0.009161 0.073771 0.000457 0.014736
Vacuole 0.042319 0.071528 0.058438 0.110264 0.032823 0.056363 0.071306 0.116802 0.040628 0.063150 0.069103 0.112505
Vacuole Periphery 0.003275 0.007571 0.000244 0.001997 0.002199 0.004320 0.000270 0.004397 0.003083 0.005775 0.000265 0.002820

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -10.70 -5.13 -9.10 -7.34 -7.62 -8.65 -2.64 -4.01 -0.85 -3.51 -13.02 -4.62 -9.71 -7.39 -9.12
Bud Neck -5.07 19.04 4.86 9.63 -15.66 -9.70 4.98 1.11 15.82 -8.62 -8.26 19.42 6.28 16.18 -17.41
Bud Site -6.26 1.53 -7.48 -3.59 -6.05 -12.97 1.20 -4.78 0.97 -5.08 -7.78 5.60 -7.77 -3.21 -11.54
Cell Periphery 8.89 8.40 7.03 -6.99 -4.40 0.66 0.48 -4.30 -5.07 -4.90 9.09 9.08 6.79 -9.38 -8.93
Cytoplasm 29.67 8.01 16.53 -9.54 -0.19 18.80 0.51 10.78 -8.16 12.04 36.30 -0.59 19.27 -12.27 14.11
Cytoplasmic Foci -8.71 42.33 37.98 43.69 -7.24 -2.13 23.31 19.20 39.17 -7.93 -11.58 46.40 40.98 61.92 -7.55
Eisosomes 9.68 15.16 12.84 6.03 -5.58 -5.42 6.94 -0.40 6.10 -9.82 8.89 18.01 12.76 8.38 -12.58
Endoplasmic Reticulum 5.47 -5.36 -11.96 -20.07 -3.51 -0.37 -11.09 -13.31 -13.52 -4.63 6.00 -2.37 -12.34 -23.21 -12.37
Endosome -17.56 11.10 7.60 20.93 -3.64 -8.37 6.30 -0.72 6.93 -6.49 -21.02 12.33 2.83 21.68 -6.85
Golgi -15.72 15.35 5.40 17.17 -2.90 -13.99 4.58 0.37 13.46 -3.26 -21.69 15.43 4.11 22.43 -4.36
Lipid Particles 7.78 18.64 18.02 9.87 -3.69 -4.33 7.66 0.67 4.25 -4.85 7.26 20.26 15.97 11.13 -6.69
Mitochondria -22.16* 16.64 12.48 28.15* -7.43 -27.15* 5.59 1.68 28.65* -3.86 -32.38* 17.51 12.08 40.88* -7.89
Mitotic Spindle -11.79 -1.53 -10.89 -5.43 -9.96 -10.74 0.22 -5.31 -0.73 -5.35 -16.43 -1.63 -12.14 -5.42 -11.74
None 9.68 8.66 8.39 -3.39 -0.92 3.49 2.39 0.02 -3.52 -2.40 9.95 9.07 8.02 -4.66 -2.20
Nuclear Periphery 0.33 -7.23 -14.85 -13.83 -7.64 0.55 -13.68 -6.13 -6.24 -3.40 1.66 -12.57 -13.20 -14.01 -7.75
Nuclear Periphery Foci -1.77 -6.03 -13.33 -12.53 0.02 2.12 -6.56 -3.63 -3.81 -1.79 -0.23 -8.70 -8.14 -8.09 -2.19
Nucleolus -2.50 8.96 2.56 4.14 -7.12 0.85 4.78 -1.85 -2.86 -6.10 -1.69 9.10 -0.69 0.85 -8.01
Nucleus 26.58 -20.76 -17.11 -37.05 12.09 12.28 -16.41 -12.48 -26.65 2.19 29.91 -25.29 -21.24 -45.60 7.21
Peroxisomes -21.83* 13.89 -2.75 18.65 -8.40 -21.12 7.43 1.21 21.58 -5.25 -32.31* 16.72 -1.92 28.00 -10.06
Vacuole -9.34 -4.40 -22.61 -16.68 -7.98 -8.09 -13.62 -15.58 -12.68 -4.58 -10.47 -13.42 -26.93 -21.93 -9.08
Vacuole Periphery -7.91 14.97 6.70 11.56 -3.40 -6.11 7.08 -1.04 1.46 -3.55 -8.64 16.33 3.60 9.67 -4.82
ORF
Human Ortholog
Description Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (11%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YDR514C

YDR514C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ydr514c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available