ORF | |
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Human Ortholog | |
Description | Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 6 | 2 | 4 | 4 | 8 | 25 |
Bud | 0 | 0 | 7 | 7 | 4 | 8 | 8 | 16 | 10 | 21 | 19 | 23 | 0 | 2 | 0 | – | 1 | 3 | 1 | 2 | 0 | 1 | 3 | 8 |
Bud Neck | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 7 | 0 | 3 | 2 | 1 | 0 | 0 | 0 | – | 3 | 0 | 1 | 0 | 0 | 0 | 1 | 14 |
Bud Site | 0 | 0 | 0 | 1 | 0 | 4 | 3 | 4 | 8 | 8 | 1 | 7 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 2 | 1 | 1 | 2 | 1 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | – | 2 | 4 | 1 | 0 | 2 | 0 | 3 | 4 |
Cytoplasm | 195 | 50 | 125 | 78 | 152 | 119 | 224 | 241 | 191 | 238 | 111 | 268 | 177 | 434 | 427 | – | 218 | 154 | 80 | 36 | 47 | 25 | 75 | 63 |
Endoplasmic Reticulum | 8 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 3 | 0 | 1 | 1 | 2 | – | 26 | 8 | 8 | 0 | 4 | 0 | 3 | 5 |
Endosome | 0 | 1 | 3 | 1 | 8 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 3 | 4 | 2 | – | 7 | 2 | 17 | 6 | 11 | 6 | 18 | 26 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 0 | 1 | 1 | 1 | 0 | 1 | 1 |
Mitochondria | 145 | 31 | 58 | 94 | 100 | 186 | 337 | 346 | 331 | 376 | 379 | 457 | 14 | 25 | 18 | – | 17 | 7 | 208 | 42 | 110 | 71 | 153 | 192 |
Nucleus | 0 | 0 | 1 | 0 | 1 | 3 | 4 | 8 | 2 | 3 | 2 | 7 | 0 | 0 | 1 | – | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 2 |
Nucleolus | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 3 | 1 | 1 | 0 | 2 | 0 | 0 | 2 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | 3 | 0 | 2 | 0 | 0 | 3 |
SpindlePole | 2 | 0 | 1 | 5 | 7 | 14 | 31 | 26 | 17 | 23 | 10 | 28 | 1 | 0 | 1 | – | 5 | 4 | 8 | 2 | 8 | 7 | 17 | 13 |
Vac/Vac Membrane | 9 | 5 | 12 | 6 | 13 | 17 | 11 | 8 | 6 | 10 | 7 | 7 | 19 | 21 | 5 | – | 24 | 10 | 19 | 13 | 10 | 5 | 14 | 23 |
Unique Cell Count | 306 | 76 | 179 | 162 | 244 | 277 | 511 | 554 | 463 | 516 | 442 | 616 | 202 | 462 | 443 | 258 | 174 | 370 | 111 | 213 | 133 | 312 | 397 | |
Labelled Cell Count | 361 | 88 | 209 | 197 | 289 | 357 | 622 | 663 | 572 | 685 | 537 | 803 | 215 | 487 | 460 | 307 | 193 | 370 | 111 | 213 | 133 | 312 | 397 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.3 | 3.7 | 3.7 | 3.6 | 3.7 | 4.0 | 2.6 | 3.0 | 2.9 | 2.6 | 2.6 | 2.6 | 4.5 | 4.2 | 4.1 | 4.4 | 5.0 | 5.3 | 4.2 | 4.3 | 4.8 |
Std Deviation (1e-4) | 0.4 | 1.1 | 1.2 | 1.0 | 1.3 | 6.4 | 0.9 | 1.1 | 1.1 | 0.8 | 0.5 | 1.2 | 1.6 | 1.1 | 0.9 | 1.4 | 1.8 | 1.7 | 1.7 | 1.9 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.04 | 0.1 | -0.52 | -0.33 | -0.39 | -0.54 | -0.51 | -0.54 | 0.27 | 0.16 | 0.12 | 0.23 | 0.43 | 0.51 | 0.16 | 0.19 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | 1.0 | -0.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.486 | -0.2294 | -0.2943 | -0.6879 | -0.2395 | -0.3158 | 0.5555 | 0.7915 | 0.5953 | 0.7756 | 0.7432 | 0.8764 | 0.5333 | 1.501 | 0.6686 | 1.0638 | 0.9683 | 0.7284 |
Actin | 0.0222 | 0.0174 | 0.0249 | 0.0148 | 0.0171 | 0.0138 | 0.0205 | 0.0148 | 0.0236 | 0.0439 | 0.0047 | 0.0062 | 0.0315 | 0.0113 | 0.0127 | 0.0222 | 0.0441 | 0.0081 |
Bud | 0.0011 | 0.0046 | 0.002 | 0.003 | 0.0045 | 0.0017 | 0.001 | 0.0129 | 0.0012 | 0.0057 | 0.0005 | 0.0006 | 0.0037 | 0.0028 | 0.0013 | 0.0058 | 0.0027 | 0.0005 |
Bud Neck | 0.0119 | 0.002 | 0.0018 | 0.0006 | 0.0009 | 0.017 | 0.0016 | 0.0003 | 0.0014 | 0.0023 | 0.0024 | 0.0105 | 0.0087 | 0.0009 | 0.0008 | 0.0033 | 0.003 | 0.0039 |
Bud Periphery | 0.0023 | 0.0067 | 0.0038 | 0.0107 | 0.0119 | 0.006 | 0.0026 | 0.0026 | 0.0029 | 0.0263 | 0.0012 | 0.0011 | 0.0062 | 0.0033 | 0.0033 | 0.008 | 0.007 | 0.0009 |
Bud Site | 0.0103 | 0.038 | 0.0119 | 0.006 | 0.0043 | 0.0023 | 0.0047 | 0.0068 | 0.0085 | 0.0022 | 0.0012 | 0.0017 | 0.0196 | 0.0307 | 0.0045 | 0.005 | 0.0021 | 0.001 |
Cell Periphery | 0.0015 | 0.0052 | 0.0011 | 0.0085 | 0.0012 | 0.0007 | 0.001 | 0.0011 | 0.0013 | 0.0005 | 0.0009 | 0.0005 | 0.0026 | 0.002 | 0.0019 | 0.0013 | 0.0005 | 0.0009 |
Cytoplasm | 0.1337 | 0.2561 | 0.1959 | 0.2448 | 0.2446 | 0.2009 | 0.1376 | 0.1335 | 0.214 | 0.1744 | 0.1555 | 0.2124 | 0.1471 | 0.1716 | 0.2233 | 0.2215 | 0.1328 | 0.2252 |
Cytoplasmic Foci | 0.106 | 0.0994 | 0.1035 | 0.1066 | 0.0686 | 0.1738 | 0.107 | 0.0639 | 0.0764 | 0.0517 | 0.1761 | 0.1028 | 0.1676 | 0.0895 | 0.1307 | 0.1561 | 0.1468 | 0.1589 |
Eisosomes | 0.0008 | 0.0011 | 0.001 | 0.001 | 0.0004 | 0.0003 | 0.0011 | 0.0014 | 0.0007 | 0.0005 | 0.0007 | 0.0002 | 0.0014 | 0.001 | 0.0009 | 0.0008 | 0.0007 | 0.0003 |
Endoplasmic Reticulum | 0.0276 | 0.0194 | 0.0135 | 0.0146 | 0.0074 | 0.0108 | 0.013 | 0.0071 | 0.0181 | 0.0262 | 0.0106 | 0.0052 | 0.0078 | 0.005 | 0.0144 | 0.0105 | 0.0073 | 0.0154 |
Endosome | 0.1052 | 0.0773 | 0.0733 | 0.0582 | 0.0777 | 0.0894 | 0.0505 | 0.0549 | 0.0387 | 0.0491 | 0.0452 | 0.0769 | 0.0717 | 0.0341 | 0.0858 | 0.0611 | 0.101 | 0.0988 |
Golgi | 0.0315 | 0.0182 | 0.0154 | 0.0154 | 0.0288 | 0.0211 | 0.0174 | 0.0042 | 0.009 | 0.032 | 0.0095 | 0.0119 | 0.0206 | 0.0215 | 0.022 | 0.0156 | 0.0378 | 0.0201 |
Lipid Particles | 0.0421 | 0.0127 | 0.0242 | 0.02 | 0.0239 | 0.0306 | 0.0391 | 0.0146 | 0.008 | 0.0064 | 0.0555 | 0.0237 | 0.0425 | 0.0213 | 0.015 | 0.0212 | 0.0124 | 0.0142 |
Mitochondria | 0.0466 | 0.0664 | 0.0905 | 0.0799 | 0.1393 | 0.0779 | 0.0429 | 0.0136 | 0.0595 | 0.0965 | 0.0164 | 0.0456 | 0.08 | 0.076 | 0.1012 | 0.0997 | 0.1287 | 0.0897 |
None | 0.3657 | 0.3272 | 0.3646 | 0.3143 | 0.3008 | 0.254 | 0.4901 | 0.6316 | 0.502 | 0.449 | 0.4322 | 0.4577 | 0.3054 | 0.5035 | 0.3335 | 0.32 | 0.28 | 0.3014 |
Nuclear Periphery | 0.0153 | 0.0051 | 0.0076 | 0.0097 | 0.0113 | 0.0127 | 0.0048 | 0.0019 | 0.0044 | 0.0071 | 0.0017 | 0.0023 | 0.0032 | 0.0014 | 0.0067 | 0.0029 | 0.0069 | 0.0035 |
Nucleolus | 0.0095 | 0.0014 | 0.002 | 0.0009 | 0.0009 | 0.0016 | 0.0016 | 0.0007 | 0.0011 | 0.0004 | 0.0013 | 0.0008 | 0.0018 | 0.0009 | 0.001 | 0.0008 | 0.0005 | 0.001 |
Nucleus | 0.0124 | 0.0119 | 0.0142 | 0.0081 | 0.0363 | 0.0159 | 0.0132 | 0.0025 | 0.0042 | 0.0024 | 0.0013 | 0.0053 | 0.0055 | 0.0022 | 0.0059 | 0.0036 | 0.0024 | 0.0152 |
Peroxisomes | 0.0257 | 0.0066 | 0.0274 | 0.0636 | 0.0054 | 0.0418 | 0.0317 | 0.0042 | 0.0154 | 0.0109 | 0.0675 | 0.0209 | 0.0517 | 0.0139 | 0.0212 | 0.0295 | 0.0724 | 0.0233 |
Punctate Nuclear | 0.0106 | 0.0078 | 0.0113 | 0.0036 | 0.003 | 0.0109 | 0.0069 | 0.0012 | 0.0034 | 0.0012 | 0.0098 | 0.0024 | 0.0105 | 0.0021 | 0.0047 | 0.0028 | 0.0046 | 0.0088 |
Vacuole | 0.0133 | 0.0128 | 0.0079 | 0.0111 | 0.0078 | 0.0133 | 0.0097 | 0.0239 | 0.0048 | 0.0072 | 0.005 | 0.0091 | 0.009 | 0.0041 | 0.0067 | 0.0061 | 0.0039 | 0.0067 |
Vacuole Periphery | 0.0049 | 0.0026 | 0.0024 | 0.0045 | 0.0036 | 0.0035 | 0.0021 | 0.0022 | 0.0014 | 0.0041 | 0.0009 | 0.0023 | 0.0018 | 0.0012 | 0.0024 | 0.0023 | 0.0022 | 0.0024 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.499 | 10.1898 | 7.5613 | 9.9336 | 8.3748 | 4.557 | 11.6767 | 9.9671 | 10.4055 | 10.1811 |
Translational Efficiency | 2.1541 | 1.7299 | 2.1229 | 1.3298 | 1.3831 | 3.4835 | 1.421 | 1.2473 | 1.1334 | 1.4623 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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