Standard name
Human Ortholog
Description Ferulic acid decarboxylase; involved in the decarboxylation of aromatic carboxylic acids such as phenylacrylic (cinnamic) acid, ferulic acid and coumaric acid and formation of the corresponding vinyl derivatives; overexpression of both Pad1p and Fdc1p increases decarboxylase activity due to the Pad1p-catalyzed formation of a diffusible cofactor required for Fdc1p activity; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm in an HTP study

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.97 0.98 0.96 0.88 0.84 0.76 0.72 0.67 0.67 0.62 0.98 0.99 0.98 0.92 0.87 0.87 0.9 0.9 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.15 0.17 0.21 0.31 0.39 0.35 0.46 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.07 0.06 0.08 0.06 0.06 0.08 0.08 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 1 1 0 0 0 1 0 1 1 1 0 1 1
Bud 0 4 0 0 0 0 3 4 3 5 3 3 1 1 0 3 16 3 0 3 15
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0
Cell Periphery 0 2 2 0 1 3 4 6 7 5 4 10 1 2 4 0 0 0 0 0 0
Cytoplasm 192 392 219 140 237 323 387 355 291 319 206 197 187 393 471 170 352 206 88 213 229
Endoplasmic Reticulum 0 0 2 0 1 1 1 7 12 14 7 0 0 1 2 0 1 4 2 2 4
Endosome 0 0 0 0 0 0 2 2 0 0 0 0 0 2 1 0 4 2 0 0 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1
Mitochondria 8 6 2 4 7 55 79 97 124 187 109 147 1 1 1 2 4 4 0 2 3
Nucleus 0 2 4 1 0 0 1 9 10 6 4 7 3 1 4 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 0 1 1 0 5 3 1 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 1 1 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 2 1 0 1 1 0 0 0 0 1 8 0 0 0 1
Vac/Vac Membrane 0 2 0 4 9 24 30 36 23 29 24 25 0 3 3 0 6 7 3 6 9
Unique Cell Count 194 400 226 143 247 367 462 469 404 476 308 318 190 398 480 186 405 238 98 237 277
Labelled Cell Count 200 408 230 149 255 406 511 521 471 573 362 391 193 405 486 186 405 238 98 237 277


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 7.3 7.1 7.5 7.8 6.9 6.4 6.5 6.1 5.7 5.9 5.5 8.8 9.0 8.8 7.5 8.3 9.0
Std Deviation (1e-4) 2.0 2.5 2.1 2.1 2.5 2.2 2.4 2.1 2.0 2.0 2.0 2.0 2.6 2.6 2.7 2.5 2.6 2.7
Intensity Change (Log2) 0.08 0.13 -0.05 -0.15 -0.13 -0.21 -0.31 -0.27 -0.36 0.31 0.35 0.3 0.08 0.23 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.6 -0.6 -3.8 -5.0 -6.9 -7.6 -8.7 -8.5 -9.5 1.0 1.6 1.0
Endoplasmic Reticulum 0 0 0 0 0 1.7 1.7 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.7 6.2 7.0 9.0 10.7 9.7 11.7 0 0 0
Nucleus 0 0 0 0 0 0.6 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.9 3.9 4.3 3.7 3.8 4.3 4.3 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9207 2.8818 2.1312 2.3309 1.9098 2.2369 2.8731 4.5713 3.105 2.6233 2.28 3.0067 4.1595 5.5812 4.5698 5.9661 3.985 4.8308
Actin 0.0118 0.0005 0.0008 0.0005 0.0001 0.002 0.0331 0.0007 0.0076 0.0083 0.0108 0.0175 0.0111 0.0009 0.0298 0.0006 0.0013 0.0163
Bud 0.0002 0.0001 0.0019 0.0004 0 0.0002 0.0009 0.0002 0.0015 0.0061 0.0004 0.0026 0.0007 0.0007 0.0008 0.0006 0.0013 0.0005
Bud Neck 0.0011 0.0002 0.0029 0.0004 0.0002 0.0011 0.0009 0.0001 0.0002 0.0005 0.0005 0.0013 0.0012 0.0003 0.0034 0.0003 0.0004 0.0008
Bud Periphery 0.0001 0.0001 0.0028 0.0005 0 0.0002 0.0014 0.0002 0.0007 0.0079 0.0004 0.0014 0.0025 0.0004 0.0014 0.0043 0.0049 0.0004
Bud Site 0.0009 0.0005 0.0051 0.0002 0 0.0001 0.0016 0.0006 0.0061 0.0018 0.0005 0.0003 0.0058 0.0028 0.0009 0.0001 0.0001 0.0005
Cell Periphery 0.0001 0.0002 0.0004 0.0001 0 0.0001 0.0002 0.0001 0.0001 0.0004 0.0001 0.0001 0.0005 0.0001 0.0003 0.0001 0.0002 0
Cytoplasm 0.7092 0.7699 0.7591 0.7797 0.6928 0.7485 0.5234 0.715 0.5462 0.4869 0.5699 0.5757 0.739 0.8099 0.708 0.7607 0.6301 0.7554
Cytoplasmic Foci 0.0057 0.0056 0.0082 0.0035 0.0036 0.0059 0.03 0.0123 0.0056 0.008 0.0244 0.0066 0.0267 0.0087 0.0112 0.003 0.0042 0.0134
Eisosomes 0.0001 0.0003 0.0002 0 0 0.0001 0.0004 0 0.0002 0.0002 0.0001 0.0002 0.0002 0.0001 0.0003 0.0001 0.0001 0
Endoplasmic Reticulum 0.0044 0.0089 0.0041 0.0065 0.0039 0.0052 0.0043 0.0015 0.0018 0.0037 0.0025 0.0017 0.0066 0.0028 0.0094 0.0006 0.0023 0.0024
Endosome 0.004 0.0026 0.0023 0.0014 0.0013 0.0064 0.0144 0.0018 0.0038 0.0022 0.0031 0.0027 0.0104 0.0012 0.0049 0.0012 0.0008 0.0037
Golgi 0.002 0.0005 0.0004 0.0001 0.0001 0.0005 0.0079 0.0003 0.0015 0.0007 0.0024 0.0021 0.0019 0.0002 0.0024 0.0001 0.0001 0.0035
Lipid Particles 0.0019 0.0052 0.0008 0.0002 0.0006 0.0008 0.0061 0.0001 0.0036 0.0208 0.0044 0.0028 0.0032 0.0001 0.0102 0 0.0002 0.0071
Mitochondria 0.0003 0.0004 0.0005 0.0003 0.0001 0.0011 0.0072 0.0002 0.0005 0.0017 0.0284 0.0008 0.0006 0.0005 0.0012 0.0006 0.0004 0.0015
None 0.2416 0.1961 0.2028 0.1891 0.2917 0.2017 0.3311 0.2631 0.4086 0.4128 0.3369 0.3683 0.1627 0.1546 0.1749 0.2143 0.341 0.1682
Nuclear Periphery 0.003 0.0024 0.0012 0.0041 0.0012 0.0043 0.0046 0.0005 0.0021 0.0029 0.0008 0.0014 0.0028 0.0013 0.0048 0.0011 0.0016 0.0008
Nucleolus 0.0002 0.0001 0.0002 0.0001 0 0.0009 0.0003 0 0.0001 0.0106 0.0002 0.0001 0.0013 0.0001 0.0003 0 0.0001 0
Nucleus 0.0059 0.0038 0.0028 0.0093 0.003 0.0108 0.0051 0.0015 0.0035 0.0142 0.0019 0.0037 0.0042 0.0126 0.0149 0.0111 0.0089 0.0025
Peroxisomes 0.0011 0.0003 0.0003 0.0002 0.0001 0.0002 0.022 0.0005 0.0039 0.0039 0.0098 0.0075 0.0108 0.0003 0.0183 0.0001 0.0002 0.0195
Punctate Nuclear 0.0035 0.0004 0.0008 0.0009 0.0006 0.0075 0.0036 0.0007 0.0016 0.0035 0.0012 0.0024 0.0061 0.0015 0.0016 0.0005 0.0007 0.0029
Vacuole 0.0024 0.0016 0.0021 0.0021 0.0004 0.0018 0.0011 0.0004 0.0006 0.0025 0.0009 0.0005 0.0013 0.0008 0.001 0.0004 0.0009 0.0004
Vacuole Periphery 0.0003 0.0002 0.0002 0.0003 0.0001 0.0006 0.0004 0.0001 0.0002 0.0004 0.0003 0.0002 0.0002 0.0001 0.0003 0.0001 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.0013 16.9615 17.9111 19.1444 20.8158 26.257 19.6233 24.708 25.6786 30.922
Translational Efficiency 0.845 0.6922 0.713 0.8343 0.786 0.9837 1.0283 0.7695 0.965 0.9817

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Ferulic acid decarboxylase; involved in the decarboxylation of aromatic carboxylic acids such as phenylacrylic (cinnamic) acid, ferulic acid and coumaric acid and formation of the corresponding vinyl derivatives; overexpression of both Pad1p and Fdc1p increases decarboxylase activity due to the Pad1p-catalyzed formation of a diffusible cofactor required for Fdc1p activity; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm in an HTP study
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Fdc1

Fdc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fdc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available