Standard name
Human Ortholog
Description Subunit of the Iml1p/SEACIT complex; SEACIT (Iml1p-Npr2p-Npr3p) is a subcomplex of the SEA complex, a coatomer-related complex that associates dynamically with the vacuole; Npr2p may have a structural or regulatory role, supporting Iml1p function as a GAP for the Rag family GTPase Gtr1p, and resulting in inhibition of TORC1 signaling in response to amino acid deprivation; SEACIT is required for non-nitrogen-starvation-induced autophagy; homolog of human tumor suppressor NPRL2
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.91 0.85 0.95 0.94 0.89 0.87 0.88 0.89 0.82 0.83 0.69 0.83 0.94 0.98 0.95 – – – 0.58 0.54 0.3 0.64 0.68 0.63
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 – – – 0 0 0 0 0 0
Endosome 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.1 0.14 0.08 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0 0.1 0.08 0.25 0.12 0.44 0.28 0 0 0 – – – 0.13 0.08 0.17 0.06 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0.12 0.06 0 0.13 0.09 0.06 0 0.06 0 0 0 0 0 0 – – – 0.12 0.17 0.35 0.15 0.16 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 1 1 0 2 2 0 0 0 0 – – – 0 2 0 0 0 2
Bud 0 0 0 1 2 0 1 6 2 6 9 2 0 0 0 – – – 0 0 1 0 0 3
Bud Neck 0 0 1 1 0 2 3 4 2 6 2 2 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 1 0 0 0 1 0 0 3 0 1 0 0 1 – – – 0 0 0 0 0 0
Cytoplasm 121 149 63 215 317 296 418 425 170 272 197 334 16 83 154 – – – 87 108 30 15 88 122
Endoplasmic Reticulum 2 1 0 5 1 1 5 0 0 0 6 1 1 0 0 – – – 1 0 1 0 0 0
Endosome 1 9 0 3 8 8 11 11 2 5 0 3 0 2 2 – – – 15 27 7 0 5 10
Golgi 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 – – – 1 2 0 0 0 0
Mitochondria 8 3 0 0 0 15 47 40 52 40 126 110 0 0 0 – – – 19 15 17 1 6 6
Nucleus 1 1 1 0 0 3 2 2 2 6 1 1 0 0 1 – – – 0 0 1 0 0 1
Nuclear Periphery 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 1 0 1 0 1 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 1 2 0 3 0 3 6 9 3 7 1 5 0 0 0 – – – 2 3 0 0 3 6
Vac/Vac Membrane 3 21 4 9 46 29 27 15 13 10 10 7 0 0 7 – – – 18 33 35 3 20 35
Unique Cell Count 133 175 66 229 356 340 475 479 207 326 287 400 17 85 162 152 200 100 24 130 195
Labelled Cell Count 137 187 70 240 374 359 523 514 246 357 354 466 17 85 165 152 200 100 24 130 195


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.7 5.5 5.2 5.9 4.8 4.8 5.0 4.1 4.2 3.9 4.2 4.5 5.4 5.2 – – – – 4.8 5.4
Std Deviation (1e-4) 0.6 1.2 1.3 1.2 1.8 1.4 1.3 1.2 1.3 0.9 1.0 1.3 0.7 1.1 1.2 – – – – 1.1 1.2
Intensity Change (Log2) – – – -0.09 0.1 -0.2 -0.2 -0.13 -0.42 -0.38 -0.52 -0.39 -0.3 -0.04 -0.09 – – – – -0.2 -0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0463 0.2101 0.1766 -0.0907 0.3437 -0.1217 -0.0479 0.6919 0.4591 0.6426 0.7838 0.1467 -1.3362 -1.0193 -1.1813 -0.9332 -0.9202 -1.2982
Actin 0.0258 0.001 0.0362 0.0013 0.0428 0.0235 0.0205 0.0009 0.0014 0.0354 0.0267 0.0088 0.0332 0.0001 0.0072 0.0002 0.0074 0.0024
Bud 0.0004 0.0031 0.0027 0.0019 0.0049 0.0009 0.0008 0.0003 0.0002 0.0017 0.0009 0.0013 0.001 0.0002 0.0015 0.0002 0.0004 0.0035
Bud Neck 0.0175 0.0007 0.0016 0.0003 0.0034 0.0006 0.0016 0.0001 0.0001 0.0002 0.0006 0.0012 0.0032 0.0001 0.0006 0.0001 0.0003 0.002
Bud Periphery 0.0007 0.0087 0.0042 0.0014 0.0084 0.0054 0.0017 0.0003 0.0002 0.0014 0.002 0.0031 0.002 0.0002 0.0017 0.0001 0.0005 0.0035
Bud Site 0.0052 0.0072 0.0146 0.0007 0.0519 0.0002 0.0094 0.001 0.0004 0.0078 0.0009 0.0005 0.0071 0.0005 0.0097 0 0.0004 0.0099
Cell Periphery 0.0004 0.0004 0.0009 0.0003 0.0024 0.0003 0.0006 0.0002 0.0001 0.0001 0.0002 0.0002 0.0006 0.0001 0.0002 0.0001 0.0001 0.0005
Cytoplasm 0.1276 0.2204 0.1896 0.3533 0.2042 0.3515 0.1684 0.347 0.2992 0.2289 0.0655 0.3938 0.1335 0.3381 0.2201 0.1597 0.2141 0.2978
Cytoplasmic Foci 0.0815 0.0264 0.048 0.0267 0.0923 0.04 0.0367 0.0313 0.0298 0.0205 0.0541 0.0329 0.0603 0.0456 0.0369 0.0535 0.0318 0.0405
Eisosomes 0.0007 0.0002 0.0006 0.0006 0.0006 0.0007 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001 0.0009 0.0001 0.0002 0.0002 0.0001 0.0005
Endoplasmic Reticulum 0.0155 0.006 0.0053 0.0056 0.0042 0.0056 0.006 0.0041 0.0077 0.0007 0.0019 0.0036 0.0055 0.0025 0.0022 0.0007 0.0036 0.0044
Endosome 0.0394 0.0499 0.0529 0.0577 0.0669 0.0619 0.0576 0.0766 0.0772 0.0887 0.2139 0.0881 0.0477 0.0497 0.0459 0.0869 0.1334 0.0844
Golgi 0.0157 0.0026 0.0201 0.0096 0.0631 0.01 0.012 0.0018 0.0028 0.0052 0.1088 0.0056 0.0132 0.0014 0.0082 0.0004 0.0378 0.0052
Lipid Particles 0.0381 0.0034 0.0081 0.0034 0.0322 0.0054 0.0255 0.0034 0.0033 0.0201 0.025 0.0024 0.0214 0.0051 0.01 0.0024 0.0156 0.0031
Mitochondria 0.0036 0.0024 0.0456 0.0027 0.0829 0.0264 0.006 0.0023 0.0117 0.0112 0.0312 0.0303 0.0216 0.0015 0.0143 0.0008 0.0162 0.0278
None 0.5312 0.6205 0.5264 0.5019 0.2683 0.4341 0.6151 0.4725 0.5192 0.5446 0.4277 0.3797 0.6013 0.5183 0.6111 0.6359 0.3844 0.4821
Nuclear Periphery 0.0069 0.0038 0.0059 0.0058 0.0049 0.0027 0.0073 0.002 0.0051 0.001 0.0015 0.0025 0.0095 0.0013 0.0028 0.0009 0.0319 0.0022
Nucleolus 0.0019 0.0051 0.0004 0.0004 0.0029 0.0005 0.0013 0.0003 0.0003 0.0002 0.0002 0.0002 0.0009 0.0003 0.0006 0.0004 0.0019 0.0004
Nucleus 0.0041 0.0024 0.0024 0.0049 0.0028 0.0024 0.0035 0.0016 0.0031 0.0006 0.0009 0.0013 0.0018 0.0012 0.0013 0.0023 0.0078 0.0025
Peroxisomes 0.0525 0.001 0.0088 0.0005 0.0075 0.0031 0.0068 0.0102 0.0005 0.0036 0.0169 0.0141 0.0164 0.0061 0.01 0.0003 0.023 0.0009
Punctate Nuclear 0.0081 0.0018 0.0024 0.0022 0.01 0.0016 0.0052 0.0009 0.0009 0.0004 0.0007 0.0005 0.0067 0.0028 0.0012 0.0004 0.0052 0.0014
Vacuole 0.0213 0.0299 0.0186 0.0143 0.0364 0.0192 0.0119 0.0362 0.0286 0.0216 0.0163 0.0242 0.0094 0.0227 0.0113 0.0465 0.0747 0.0211
Vacuole Periphery 0.0016 0.0032 0.0046 0.0045 0.0069 0.0042 0.0019 0.007 0.0083 0.0063 0.0039 0.0058 0.0027 0.0022 0.0029 0.0081 0.0096 0.004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.5533 6.9388 8.7135 14.5639 5.419 12.5317 10.5237 12.9307 12.3587 14.1577
Translational Efficiency 0.6598 0.5394 0.611 0.3435 0.8391 0.3798 0.6205 0.4862 0.553 0.4872

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
239 1297 1623 1255 1513 1060 1520 98 1752 2357 3143 1353

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 574.02 623.51 834.84 808.20 715.16 696.27 861.88 843.30 695.91 656.23 847.92 810.74
Standard Deviation 60.41 100.20 120.07 118.15 80.35 97.77 97.59 116.32 91.76 105.52 110.60 118.36
Intensity Change Log 2 — 0.119312 0.540399 0.493611 — -0.038619 0.269221 0.237780 — 0.033844 0.396295 0.357323

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000244 0.000641 0.000351 0.000973 0.000147 0.000543 0.000145 0.000310 0.000160 0.000597 0.000252 0.000925
Bud Neck 0.036737 0.069183 0.010072 0.010662 0.017178 0.054961 0.007092 0.003208 0.019846 0.062787 0.008631 0.010122
Bud Site 0.005817 0.034707 0.008829 0.040806 0.005297 0.035903 0.012090 0.004180 0.005368 0.035245 0.010406 0.038153
Cell Periphery 0.000211 0.000238 0.000122 0.000180 0.000092 0.000174 0.000109 0.000155 0.000108 0.000210 0.000116 0.000178
Cytoplasm 0.354457* 0.291967* 0.233023* 0.474315* 0.448330* 0.321973* 0.402415* 0.384096* 0.435524* 0.305462* 0.314943* 0.467780*
Cytoplasmic Foci 0.298710* 0.188900* 0.003637 0.013728 0.213219* 0.237557* 0.007802 0.043336 0.224881* 0.210782* 0.005651 0.015872
Eisosomes 0.000343 0.000165 0.000019 0.000037 0.000102 0.000173 0.000026 0.000084 0.000135 0.000169 0.000022 0.000040
Endoplasmic Reticulum 0.002280 0.001464 0.003893 0.002666 0.001654 0.000806 0.002336 0.003619 0.001739 0.001168 0.003140 0.002735
Endosome 0.047856 0.047379 0.002060 0.004875 0.027833 0.069511 0.002781 0.005201 0.030564 0.057332 0.002409 0.004899
Golgi 0.004102 0.009045 0.000101 0.001527 0.001755 0.009261 0.000052 0.000082 0.002075 0.009142 0.000077 0.001422
Lipid Particles 0.013727 0.010283 0.000580 0.000937 0.006069 0.006997 0.000943 0.002137 0.007113 0.008805 0.000756 0.001024
Mitochondria 0.010544 0.032724 0.001011 0.001736 0.003165 0.027809 0.000880 0.000198 0.004172 0.030513 0.000948 0.001625
Mitotic Spindle 0.000060 0.007742 0.009317 0.022034 0.001640 0.013849 0.002481 0.002887 0.001424 0.010488 0.006011 0.020647
None 0.001469 0.006182 0.006507 0.005059 0.001962 0.001959 0.006181 0.004245 0.001895 0.004283 0.006349 0.005000
Nuclear Periphery 0.000370 0.000835 0.001808 0.001955 0.000584 0.000385 0.002143 0.001482 0.000555 0.000633 0.001970 0.001921
Nuclear Periphery Foci 0.000638 0.000778 0.000842 0.001342 0.000510 0.001333 0.000847 0.001694 0.000528 0.001028 0.000844 0.001367
Nucleolus 0.000758 0.005970 0.000813 0.000323 0.001217 0.002008 0.000492 0.000335 0.001154 0.004188 0.000657 0.000324
Nucleus 0.087086 0.122929 0.693863* 0.324708* 0.158766* 0.070997 0.489386* 0.377518* 0.148988* 0.099574 0.594975* 0.328533*
Peroxisomes 0.007261 0.030418 0.000380 0.001559 0.006201 0.022946 0.000172 0.001929 0.006345 0.027058 0.000279 0.001586
Vacuole 0.122843 0.132662 0.022190 0.089077 0.102413 0.114203 0.061377 0.163168 0.105200 0.124360 0.041142 0.094444
Vacuole Periphery 0.004487 0.005787 0.000585 0.001502 0.001868 0.006653 0.000248 0.000135 0.002225 0.006176 0.000422 0.001403

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.50 -2.07 -1.85 0.24 -0.85 -4.73 -1.43 -3.62 1.92 -3.11 -8.24 -5.64 -2.78 0.04 -1.69
Bud Neck -4.57 4.17 4.85 16.50 1.97 -10.32 5.87 10.49 15.08 3.42 -14.92 6.24 7.46 20.73 1.38
Bud Site -12.70 -1.69 -5.95 3.18 -4.72 -10.50 -1.89 0.45 10.26 1.98 -16.41 -2.66 -6.17 4.24 -4.72
Cell Periphery -0.51 3.63 2.89 2.97 -1.10 -5.48 -1.32 -3.64 0.15 -3.01 -5.59 -0.98 -3.32 3.09 -2.54
Cytoplasm 3.67 9.01 -1.27 -8.29 -18.81 11.51 10.71 5.39 0.92 1.72 15.31 21.05 6.35 -7.35 -11.88
Cytoplasmic Foci 7.27 20.97 20.30 33.74 -10.25 -2.89 39.73 21.18 21.95 -3.09 2.20 44.66 42.39 47.29 -9.37
Eisosomes 5.18 9.50 8.74 17.09 -10.89 -5.83 12.78 -0.23 4.50 -6.88 -3.82 15.62 11.51 18.06 -11.77
Endoplasmic Reticulum 1.02 -4.48 -2.02 -7.84 5.51 3.80 -6.75 -4.56 -5.80 -2.42 2.40 -11.34 -7.41 -11.04 2.42
Endosome 0.19 7.96 7.76 16.68 -1.71 -8.37 13.21 11.40 14.08 -2.41 -8.42 15.43 14.58 21.18 -1.79
Golgi -2.60 2.80 2.36 7.77 -2.26 -7.39 7.43 7.27 9.31 -2.79 -9.01 7.16 3.95 11.15 -2.37
Lipid Particles 1.92 8.08 7.73 9.92 -6.64 -1.27 12.27 6.92 5.98 -4.83 -2.34 14.86 13.56 12.62 -6.62
Mitochondria -5.03 3.00 3.01 11.51 0.15 -8.37 3.61 4.72 9.61 6.31 -12.42 4.56 4.33 14.75 -0.10
Mitotic Spindle -5.68 -5.82 -6.54 -2.77 -1.82 -5.48 -2.30 -0.90 3.47 0.11 -6.75 -5.05 -5.80 -1.53 -3.12
None -4.26 -8.41 -4.87 1.29 2.10 -0.25 -4.37 -2.08 -1.92 1.34 -3.65 -7.71 -4.83 -0.41 1.71
Nuclear Periphery -2.52 -8.56 -9.94 -7.10 -0.85 2.62 -21.32 -7.77 -9.03 5.42 -0.74 -16.23 -10.23 -9.32 0.40
Nuclear Periphery Foci -0.44 -1.38 -2.52 -3.20 -1.72 -1.22 -4.70 -4.25 -1.20 -3.09 -1.56 -4.48 -4.91 -1.69 -2.54
Nucleolus -6.22 -1.79 2.48 6.58 3.56 -2.46 3.12 5.37 5.75 2.61 -6.07 1.79 4.90 7.82 3.77
Nucleus -4.04 -58.61 -25.14 -26.36 33.37 14.85 -41.74 -7.09 -10.03 4.93 8.86 -69.73 -25.83 -32.90 27.44
Peroxisomes -10.55 6.43 6.01 15.28 -1.34 -8.85 7.61 5.61 12.37 -1.35 -13.89 8.57 7.55 19.78 -2.22
Vacuole -1.46 8.18 -4.19 -4.25 -20.38 -2.13 1.99 -5.70 -5.24 -6.06 -4.70 10.22 -10.37 -7.18 -17.41
Vacuole Periphery -0.85 3.93 3.64 8.34 -0.73 -5.84 9.58 10.07 8.18 2.39 -7.55 8.44 4.95 10.36 -1.22
Standard name
Human Ortholog
Description Subunit of the Iml1p/SEACIT complex; SEACIT (Iml1p-Npr2p-Npr3p) is a subcomplex of the SEA complex, a coatomer-related complex that associates dynamically with the vacuole; Npr2p may have a structural or regulatory role, supporting Iml1p function as a GAP for the Rag family GTPase Gtr1p, and resulting in inhibition of TORC1 signaling in response to amino acid deprivation; SEACIT is required for non-nitrogen-starvation-induced autophagy; homolog of human tumor suppressor NPRL2
Localization
Cell Percentages cytoplasm (25%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Npr2

Npr2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Npr2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available