Standard name
Human Ortholog
Description Endoplasmic reticulum membrane protein; may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud 0 0 0 0 0.05 0.05 0.05 0.06 0.06 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.91 0.92 0.86 0.76 0.74 0.71 0.69 0.79 0.83 0.8 0.81 0.81 0.73 0.83 0.92 0.83 0.91 0.27 0.31 0.37 0.25 0.23 0.23
Endoplasmic Reticulum 0.05 0 0.06 0.16 0.19 0.17 0.11 0.09 0 0 0.07 0.18 0.24 0.13 0.08 0 0 0.23 0.26 0.11 0.23 0.36 0.38
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0 0.09 0.11 0 0 0 0 0.06 0.05 0.05 0.07 0 0 0 0 0 0.05 0.05 0.05 0.12 0.14 0.08 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0 0.05 0.11 0.05 0.06 0.06 0.06 0 0 0 0 0.09 0 0.4 0.32 0.3 0.27 0.21 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 17 13 29 30 20 13 14 9 6 25 15 2 3 1 1 0 1 2 13 15
Bud 1 3 9 16 21 33 38 35 35 43 27 7 6 5 0 0 0 1 0 1 2 6 9
Bud Neck 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 0 3 2 6 12
Bud Site 0 0 0 1 0 0 2 1 2 1 1 0 0 0 0 0 0
Cell Periphery 2 1 4 15 14 15 12 12 11 23 14 6 17 38 2 4 0 0 0 0 0 0 3
Cytoplasm 353 134 243 344 301 501 494 435 475 450 527 350 446 585 47 54 39 115 10 61 39 100 117
Endoplasmic Reticulum 19 2 18 73 78 120 79 48 19 24 44 76 143 92 4 2 1 95 8 18 37 157 191
Endosome 2 0 4 18 7 26 28 4 11 10 11 7 32 28 0 3 1 6 0 3 5 13 17
Golgi 0 0 0 0 0 0 2 0 0 0 0 0 2 2 0 0 2 2 0 1 1 4 1
Mitochondria 4 13 31 4 16 22 19 34 28 29 46 1 3 2 0 1 2 21 1 19 23 34 26
Nucleus 0 0 0 1 1 4 6 1 4 4 1 0 0 1 0 0 0 0 0 0 1 1 0
Nuclear Periphery 0 0 1 1 0 4 4 0 3 1 5 0 0 1 0 0 0 1 0 0 0 1 1
Nucleolus 0 1 0 1 0 2 6 2 0 2 1 0 1 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 2 0 0 2 0 0 1 0 1 1 4 0 0 0 0 0 1 0 1 1
Vac/Vac Membrane 12 4 1 21 10 32 76 25 33 31 40 11 5 5 2 6 0 167 11 50 42 92 99
Unique Cell Count 386 145 282 452 407 709 718 553 571 561 648 433 608 708 51 65 43 422 35 166 160 438 503
Labelled Cell Count 395 159 312 514 462 788 798 617 634 633 726 465 681 779 57 73 47 422 35 166 160 438 503


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 3.6 4.9 6.6 6.6 6.9 7.1 6.5 6.9 6.6 6.3 6.8 7.1 6.7 9.9 9.1 9.2 5.8 6.4 7.0
Std Deviation (1e-4) 0.7 0.8 1.2 1.4 1.2 1.4 1.3 1.4 1.6 1.5 1.4 1.1 1.2 1.3 1.9 1.5 1.9 1.7 1.2 1.4
Intensity Change (Log2) 0.42 0.86 0.85 0.92 0.95 0.83 0.91 0.85 0.79 0.91 0.97 0.88 1.44 1.31 1.34 0.67 0.81 0.93


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 2.0 2.1 0 0 0 0 0 2.0 0 0 0 0
Bud 0 0 1.6 1.4 1.7 2.0 1.9 2.4 1.2 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 2.5 0 0 0
Cytoplasm -1.9 -4.3 -4.7 -5.5 -5.8 -3.8 -2.8 -3.5 -3.2 -3.3 -4.9 -3.0 -0.1 -2.0 -0.4
Endoplasmic Reticulum 0 4.7 5.2 4.9 3.6 3.0 0 1.7 2.5 4.9 6.1 4.1 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 2.8 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 -2.3 -3.2 -3.7 -1.2 -1.9 -1.7 -0.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.0 0 1.0 3.0 0.9 1.5 1.4 1.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9698 1.7476 1.3387 0.9693 0.5554 1.7316 1.0831 2.0083 1.5139 1.3656 1.0274 1.5243 3.4447 4.9345 3.7871 3.8978 4.1276 3.8682
Actin 0.0125 0.0003 0.0044 0.0192 0 0.0002 0.0286 0.0097 0.0274 0.0317 0.0033 0.001 0.0251 0.0007 0.0026 0.02 0.0012 0.01
Bud 0.0004 0.0008 0.0002 0.0003 0.0002 0 0.0008 0.0034 0.0043 0.0029 0.0008 0.0001 0.0002 0.0005 0.0004 0.0002 0.0001 0.0012
Bud Neck 0.0089 0.0008 0.0027 0.0017 0.001 0.0008 0.0026 0.0005 0.0063 0.0019 0.0004 0.0009 0.0011 0.0002 0.0003 0.0002 0.0026 0.0024
Bud Periphery 0.0007 0.0014 0.0002 0.0005 0.0001 0 0.0011 0.0024 0.0022 0.0037 0.001 0.0002 0.0003 0.0003 0.0004 0.0002 0.0001 0.0012
Bud Site 0.0036 0.0043 0.0012 0.0045 0.0011 0.0001 0.0073 0.0234 0.0164 0.0096 0.0005 0.0003 0.0007 0.0032 0.0008 0.0002 0.0003 0.0016
Cell Periphery 0.0013 0.0012 0.0006 0.0009 0.0003 0.0001 0.0009 0.0011 0.0008 0.0009 0.0002 0.0004 0.0008 0.0007 0.0003 0.0002 0.0004 0.0004
Cytoplasm 0.3797 0.6229 0.4963 0.3346 0.4408 0.5183 0.3458 0.5303 0.4192 0.4352 0.4206 0.5193 0.4385 0.5675 0.5253 0.3718 0.5076 0.5383
Cytoplasmic Foci 0.0351 0.0188 0.041 0.0624 0.035 0.0364 0.0566 0.0352 0.0758 0.0402 0.0408 0.0515 0.0399 0.0363 0.0384 0.0216 0.0265 0.0224
Eisosomes 0.002 0 0.0001 0.0003 0 0 0.0002 0.0001 0.0002 0.0006 0 0 0.0003 0 0 0.0001 0 0.0001
Endoplasmic Reticulum 0.1611 0.1551 0.0862 0.0848 0.0092 0.0904 0.2086 0.1615 0.1314 0.1141 0.1745 0.1183 0.1928 0.1376 0.1449 0.1674 0.111 0.1832
Endosome 0.1009 0.0781 0.1137 0.2216 0.3013 0.1075 0.1303 0.099 0.1744 0.1676 0.2408 0.1285 0.1038 0.1 0.1455 0.15 0.1693 0.0974
Golgi 0.0132 0.002 0.0062 0.0259 0.0015 0.0022 0.0183 0.0048 0.0142 0.0223 0.0313 0.0101 0.0068 0.0034 0.0068 0.0075 0.0079 0.0067
Lipid Particles 0.0168 0.009 0.0158 0.0116 0.0014 0.0068 0.0379 0.0093 0.0101 0.0166 0.0042 0.0266 0.0134 0.0056 0.0048 0.0044 0.03 0.0022
Mitochondria 0.0054 0.0013 0.0016 0.012 0.0004 0.0006 0.0098 0.0012 0.0051 0.0409 0.0228 0.0133 0.0027 0.0008 0.0015 0.0042 0.0013 0.0016
None 0.1648 0.0512 0.174 0.0934 0.0166 0.1685 0.0843 0.0727 0.0561 0.0466 0.0117 0.0758 0.0995 0.0666 0.0603 0.183 0.0495 0.0575
Nuclear Periphery 0.0283 0.013 0.0079 0.0324 0.005 0.012 0.0159 0.0099 0.0089 0.0058 0.0053 0.0063 0.0168 0.0115 0.0115 0.0137 0.0259 0.0098
Nucleolus 0.004 0.0017 0.0002 0.0004 0.0004 0.0001 0.0006 0.0006 0.0009 0.0014 0.0001 0.0002 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001
Nucleus 0.0056 0.0037 0.002 0.004 0.0027 0.0022 0.0095 0.0032 0.0046 0.0021 0.0023 0.0028 0.003 0.0032 0.0028 0.0024 0.0082 0.0033
Peroxisomes 0.0024 0.0002 0.0094 0.0187 0.0001 0.0003 0.0136 0.0005 0.009 0.0163 0.0003 0.006 0.0028 0.0005 0.0007 0.004 0.0001 0.0005
Punctate Nuclear 0.0191 0.0008 0.0027 0.0052 0.0003 0.0009 0.0023 0.003 0.0082 0.0017 0.0007 0.001 0.0064 0.001 0.0007 0.0009 0.0006 0.0008
Vacuole 0.0311 0.0304 0.0292 0.0564 0.1746 0.0476 0.0217 0.0257 0.0206 0.0326 0.0318 0.031 0.0413 0.0568 0.047 0.041 0.0519 0.0548
Vacuole Periphery 0.003 0.0029 0.0046 0.009 0.0081 0.0049 0.0033 0.0024 0.0038 0.0052 0.0063 0.0065 0.0034 0.0034 0.0046 0.007 0.0054 0.0046

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 53.072 33.0999 52.3979 51.8942 30.4045 54.2762 46.2964 54.9645 56.989 50.1321
Translational Efficiency 1.4366 1.6038 1.21 1.1017 1.758 1.575 1.5569 1.8383 1.4242 1.5961

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1638 955 845 1254 1649 1798 1604 1649 3287 2753 2449 2903

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 710.30 774.29 1232.61 1062.35 794.00 808.60 974.75 1044.09 752.29 796.70 1063.72 1051.98
Standard Deviation 86.00 105.99 235.50 184.38 113.77 107.16 159.74 163.72 109.23 108.00 225.55 173.18
Intensity Change Log 2 0.124446 0.795216 0.580759 0.026287 0.295893 0.395035 0.073469 0.553230 0.485715

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000419 0.000790 0.005265 0.016120 0.000778 0.000370 0.005026 0.014951 0.000599 0.000516 0.005109 0.015456
Bud Neck 0.014446 0.015146 0.004664 0.012798 0.006630 0.022008 0.009667 0.012856 0.010525 0.019628 0.007941 0.012831
Bud Site 0.006883 0.013290 0.027029 0.048963 0.004558 0.012224 0.025452 0.042832 0.005716 0.012594 0.025996 0.045480
Cell Periphery 0.000417 0.000335 0.004801 0.005238 0.000646 0.000355 0.000898 0.004254 0.000532 0.000348 0.002245 0.004679
Cytoplasm 0.351645* 0.254028* 0.173730* 0.199557* 0.245553* 0.287034* 0.271178* 0.223950* 0.298421* 0.275584* 0.237555* 0.213413*
Cytoplasmic Foci 0.267015* 0.270527* 0.040787 0.084818 0.158540 0.235957* 0.098870 0.090924 0.212596* 0.247949* 0.078829 0.088286
Eisosomes 0.000529 0.000327 0.000257 0.001410 0.000754 0.000485 0.000521 0.001943 0.000642 0.000430 0.000430 0.001713
Endoplasmic Reticulum 0.056366 0.039062 0.523938* 0.246380* 0.252868* 0.037159 0.221689* 0.247692* 0.154946* 0.037819 0.325976* 0.247125*
Endosome 0.032850 0.085508 0.028659 0.039970 0.042600 0.064561 0.036532 0.040841 0.037742 0.071828 0.033815 0.040465
Golgi 0.013614 0.012098 0.009543 0.012951 0.024116 0.012205 0.009042 0.021340 0.018882 0.012168 0.009215 0.017716
Lipid Particles 0.069863 0.035729 0.052965 0.102079* 0.083328 0.044342 0.069679 0.105983* 0.076618 0.041354 0.063912 0.104296*
Mitochondria 0.010133 0.004083 0.000539 0.001280 0.007651 0.006877 0.002251 0.003820 0.008888 0.005908 0.001660 0.002723
Mitotic Spindle 0.000862 0.001442 0.002944 0.010643 0.000365 0.001898 0.004666 0.005327 0.000613 0.001739 0.004072 0.007623
None 0.003626 0.002132 0.001025 0.001602 0.003071 0.001924 0.001544 0.001278 0.003348 0.001996 0.001365 0.001418
Nuclear Periphery 0.002307 0.001756 0.001595 0.001427 0.002969 0.001231 0.002952 0.001294 0.002639 0.001413 0.002484 0.001351
Nuclear Periphery Foci 0.002745 0.003820 0.053321 0.023408 0.004191 0.001568 0.033693 0.013626 0.003471 0.002349 0.040465 0.017851
Nucleolus 0.001466 0.001093 0.000080 0.000443 0.000673 0.000943 0.000401 0.000393 0.001068 0.000995 0.000291 0.000414
Nucleus 0.063858 0.028818 0.011966 0.016871 0.029846 0.041599 0.046757 0.020404 0.046795 0.037165 0.034752 0.018878
Peroxisomes 0.005030 0.009158 0.000462 0.003894 0.004271 0.007158 0.003604 0.006155 0.004649 0.007852 0.002520 0.005178
Vacuole 0.092610 0.213782* 0.055439 0.167036 0.121050 0.214265* 0.153841 0.138016 0.106877 0.214098* 0.119888 0.150551
Vacuole Periphery 0.003315 0.007079 0.000989 0.003114 0.005542 0.005837 0.001737 0.002123 0.004432 0.006267 0.001479 0.002551

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.91 -5.99 -9.04 -8.82 -5.48 2.42 -7.35 -11.07 -11.48 -7.03 0.83 -9.66 -14.26 -14.39 -8.89
Bud Neck -0.42 7.85 1.06 1.49 -7.65 -10.21 -3.10 -6.20 5.57 -2.74 -7.66 2.97 -2.65 5.68 -5.79
Bud Site -4.74 -7.09 -12.56 -10.28 -4.69 -5.71 -9.59 -13.61 -10.08 -5.00 -6.83 -11.67 -18.41 -14.28 -6.98
Cell Periphery 1.58 -4.68 -7.18 -7.30 -0.09 5.54 -2.15 -7.85 -8.57 -7.26 5.32 -5.11 -10.66 -11.18 -4.54
Cytoplasm 10.41 17.05 16.90 5.34 -2.29 -5.54 -3.19 2.76 8.02 5.60 3.80 9.15 14.23 9.99 3.68
Cytoplasmic Foci -0.44 41.13 31.47 26.89 -10.12 -13.09 12.34 13.82 27.05 1.41 -7.33 34.17 31.93 37.71 -3.36
Eisosomes 3.71 4.55 -4.30 -5.47 -5.80 3.92 3.52 -7.18 -9.24 -9.05 4.69 4.69 -8.38 -10.37 -10.38
Endoplasmic Reticulum 3.34 -33.97 -17.81 -19.46 18.08 27.51* 4.34 1.46 -24.60 -2.76 23.63 -18.91 -10.67 -31.41 8.71
Endosome -13.71 1.95 -2.81 11.58 -4.33 -8.87 3.46 1.02 8.97 -2.11 -16.36 3.05 -1.31 14.35 -3.92
Golgi 1.22 3.27 1.34 0.01 -1.88 8.23 12.34 2.16 -5.00 -8.08 6.87 11.69 1.94 -4.14 -7.87
Lipid Particles 10.07 3.66 -5.48 -12.46 -7.79 11.92 3.90 -4.43 -13.53 -7.40 14.81 4.60 -7.18 -18.08 -10.37
Mitochondria 5.17 8.74 8.07 6.38 -4.05 0.79 5.92 4.03 3.45 -1.90 3.66 9.70 8.27 5.53 -2.06
Mitotic Spindle -1.45 -2.96 -5.39 -5.04 -3.88 -2.84 -4.37 -4.33 -2.80 -0.20 -2.93 -5.04 -6.86 -5.60 -2.75
None 1.21 3.64 2.17 0.62 -0.66 1.07 1.57 1.99 0.81 0.29 1.71 3.00 3.02 0.97 -0.07
Nuclear Periphery 1.04 1.46 1.80 0.52 0.24 3.23 -0.26 2.81 -0.47 3.98 3.42 0.05 3.34 -0.13 3.82
Nuclear Periphery Foci -1.91 -14.12 -12.20 -11.11 7.37 7.06 -17.52 -9.15 -11.96 10.05 3.77 -22.25 -15.31 -16.34 11.56
Nucleolus 1.44 7.10 4.70 3.81 -5.15 -2.15 2.83 2.90 5.26 -0.01 0.48 7.04 5.57 6.31 -2.83
Nucleus 13.62 22.59 19.91 5.28 -3.19 -5.68 -7.61 5.03 10.79 11.76 5.77 5.54 18.33 12.24 9.98
Peroxisomes -4.39 17.75 2.94 5.54 -11.03 -4.22 1.70 -3.06 1.57 -4.54 -5.89 7.35 -1.18 4.81 -7.34
Vacuole -17.07 6.76 -13.17 3.08 -17.26 -15.04 -7.76 -4.63 9.37 2.83 -23.25 -5.57 -12.62 8.84 -6.43
Vacuole Periphery -5.33 10.94 0.72 4.74 -3.35 -0.44 7.92 8.06 7.24 -1.05 -4.11 10.02 5.89 8.11 -2.86
Standard name
Human Ortholog
Description Endoplasmic reticulum membrane protein; may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p
Localization
Cell Percentages ER (63%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Erg28

Erg28


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Erg28-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available