Standard name | |
---|---|
Human Ortholog | |
Description | Fe(II)/2-oxoglutarate-dependent dioxygenase family member; catalyzes the repair of methyl-base lesions in both ss and dsDNA by oxidative demethylation; Poly(rA)-binding protein involved in mRNA poly(A) tail length and mRNA stability; role in translation termination efficiency; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; similar to human prolyl 4-hydroxylase OGFOD1; binds Fe(II) and 2-oxoglutarate |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 3 | 0 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 2 | 3 | 4 | 4 | 8 | 0 | 1 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 50 | 14 | 7 | 10 | 7 | 20 | 16 | 10 | 17 | 8 | 16 | 13 | 39 | 45 | 23 | 32 | 31 | 0 | 2 | 0 | 1 | 2 | 1 |
Endoplasmic Reticulum | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 5 | 10 | 0 | 0 | 1 | 0 | 2 | 0 |
Endosome | 1 | 1 | 1 | 0 | 1 | 2 | 2 | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 4 | 1 | 0 | 1 | 2 | 5 | 1 | 0 | 0 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 1 | 0 | 3 | 2 | 0 |
Mitochondria | 7 | 6 | 0 | 0 | 0 | 18 | 27 | 24 | 35 | 32 | 71 | 56 | 0 | 0 | 0 | 6 | 2 | 6 | 2 | 1 | 0 | 0 | 1 | 5 |
Nucleus | 386 | 348 | 264 | 207 | 233 | 257 | 336 | 310 | 275 | 278 | 244 | 359 | 216 | 190 | 227 | 154 | 150 | 185 | 382 | 437 | 278 | 166 | 260 | 205 |
Nuclear Periphery | 1 | 5 | 0 | 3 | 2 | 0 | 1 | 1 | 1 | 0 | 2 | 4 | 1 | 1 | 3 | 2 | 2 | 1 | 1 | 1 | 0 | 0 | 1 | 1 |
Nucleolus | 3 | 9 | 2 | 2 | 2 | 14 | 8 | 4 | 13 | 15 | 20 | 18 | 2 | 3 | 10 | 7 | 6 | 5 | 1 | 3 | 1 | 2 | 17 | 17 |
Peroxisomes | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 6 | 4 | 1 | 0 | 1 | 1 | 5 | 9 | 1 | 1 | 0 | 0 | 2 | 1 | 0 | 4 | 2 |
Vac/Vac Membrane | 2 | 33 | 8 | 2 | 6 | 7 | 6 | 4 | 2 | 5 | 5 | 15 | 5 | 5 | 6 | 14 | 17 | 26 | 7 | 24 | 10 | 6 | 35 | 42 |
Unique Cell Count | 401 | 433 | 284 | 215 | 253 | 285 | 376 | 345 | 307 | 321 | 289 | 421 | 237 | 237 | 287 | 203 | 207 | 248 | 402 | 487 | 303 | 185 | 332 | 288 |
Labelled Cell Count | 408 | 456 | 290 | 221 | 256 | 307 | 407 | 368 | 345 | 355 | 354 | 481 | 241 | 246 | 308 | 212 | 218 | 269 | 402 | 487 | 303 | 185 | 332 | 288 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.5 | 11.5 | 9.6 | 9.8 | 8.7 | 6.9 | 6.5 | 6.7 | 5.7 | 6.0 | 5.3 | 5.5 | 12.6 | 10.7 | 10.0 | 13.1 | 12.9 | 12.9 | 10.1 | 10.2 | 9.6 |
Std Deviation (1e-4) | 1.4 | 4.3 | 1.8 | 2.2 | 1.6 | 1.6 | 1.8 | 1.8 | 1.4 | 1.6 | 1.3 | 1.3 | 3.1 | 3.6 | 3.3 | 5.4 | 5.1 | 4.7 | 2.3 | 2.3 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.03 | -0.13 | -0.48 | -0.55 | -0.51 | -0.75 | -0.68 | -0.84 | -0.81 | 0.39 | 0.16 | 0.06 | 0.45 | 0.42 | 0.42 | 0.08 | 0.08 | -0.0 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.9 | -0.5 | -1.6 | 0.2 | -0.2 | -1.0 | 0.2 | -1.3 | -0.7 | 0.3 | 4.3 | 4.2 | 2.6 | 4.0 | 3.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.3 | 4.6 | 4.5 | 5.9 | 5.5 | 8.9 | 6.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1.6 | -0.4 | -1.2 | -1.6 | -1.4 | -1.4 | -2.6 | -3.2 | -3.1 | -0.8 | -4.3 | -4.8 | -5.3 | -6.2 | -5.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 3.0 | 0 | 0 | 2.7 | 2.9 | 3.9 | 2.8 | 0 | 0 | 2.3 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | -0.3 | -0.3 | -1.1 | -1.5 | 0 | -1.1 | -0.9 | 0.5 | -0.5 | -0.5 | -0.6 | 2.1 | 2.7 | 3.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.4019 | 9.4859 | 8.2212 | 6.7307 | 6.0771 | 8.567 | 7.083 | 8.2557 | 7.0838 | 7.0639 | 5.8625 | 7.1759 | 7.1324 | 9.522 | 8.0705 | 8.1092 | 5.676 | 8.4925 |
Actin | 0.0242 | 0 | 0.0004 | 0.0216 | 0.0054 | 0.0118 | 0.0095 | 0 | 0.0024 | 0.0092 | 0.0107 | 0.0027 | 0.0336 | 0 | 0.0195 | 0 | 0.0472 | 0.0113 |
Bud | 0.0004 | 0 | 0.0003 | 0.0004 | 0.0006 | 0.0006 | 0.0001 | 0 | 0.0003 | 0.0006 | 0.001 | 0.0002 | 0.0009 | 0 | 0.0002 | 0 | 0.005 | 0.0002 |
Bud Neck | 0.0004 | 0 | 0.0001 | 0.0027 | 0.0008 | 0.0009 | 0.0001 | 0 | 0.0046 | 0.0004 | 0.0005 | 0.0004 | 0.0008 | 0 | 0.0012 | 0 | 0.0011 | 0.0016 |
Bud Periphery | 0.0004 | 0 | 0.0008 | 0.0005 | 0.002 | 0.0014 | 0.0002 | 0 | 0.0004 | 0.0013 | 0.0018 | 0.0002 | 0.0017 | 0 | 0.0004 | 0 | 0.0097 | 0.0003 |
Bud Site | 0.0026 | 0 | 0.0005 | 0.002 | 0.0009 | 0.0008 | 0.0003 | 0.0001 | 0.0015 | 0.0012 | 0.0021 | 0.0001 | 0.0063 | 0 | 0.0027 | 0 | 0.0032 | 0.0003 |
Cell Periphery | 0.0002 | 0 | 0.0004 | 0.0004 | 0.001 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0009 | 0 | 0.0002 | 0 | 0.0003 | 0.0001 |
Cytoplasm | 0.0217 | 0 | 0.0004 | 0.0012 | 0.0006 | 0.0013 | 0.005 | 0.0003 | 0.0004 | 0.0014 | 0.0018 | 0.0033 | 0.0045 | 0 | 0.0004 | 0 | 0.003 | 0.0001 |
Cytoplasmic Foci | 0.0262 | 0 | 0.0011 | 0.014 | 0.0044 | 0.0009 | 0.0063 | 0 | 0.0008 | 0.0011 | 0.0179 | 0.0006 | 0.0169 | 0 | 0.0076 | 0 | 0.0494 | 0.0014 |
Eisosomes | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0003 | 0.0001 | 0 | 0.0007 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.003 | 0 | 0.0005 | 0.0002 | 0.0026 | 0.0003 | 0.0013 | 0 | 0.0001 | 0.0028 | 0.001 | 0.0006 | 0.0071 | 0 | 0.0002 | 0 | 0.0039 | 0.0002 |
Endosome | 0.0263 | 0 | 0.0007 | 0.0079 | 0.0738 | 0.0034 | 0.0067 | 0.0001 | 0.0011 | 0.0088 | 0.0164 | 0.0026 | 0.0222 | 0 | 0.0033 | 0 | 0.041 | 0.0044 |
Golgi | 0.0082 | 0 | 0.0008 | 0.0401 | 0.0421 | 0.0019 | 0.0012 | 0 | 0.001 | 0.0018 | 0.0152 | 0.0015 | 0.0069 | 0 | 0.0055 | 0 | 0.0416 | 0.0039 |
Lipid Particles | 0.0305 | 0 | 0.0055 | 0.005 | 0.0237 | 0.004 | 0.0068 | 0 | 0.0007 | 0.0025 | 0.022 | 0.0007 | 0.0094 | 0 | 0.0062 | 0 | 0.0149 | 0.0024 |
Mitochondria | 0.0039 | 0.0001 | 0.003 | 0.005 | 0.0487 | 0.0089 | 0.0029 | 0.0001 | 0.0016 | 0.0434 | 0.041 | 0.001 | 0.0371 | 0.0001 | 0.0054 | 0.0001 | 0.0232 | 0.0031 |
None | 0.0026 | 0 | 0.0002 | 0.0003 | 0.0007 | 0.0002 | 0.0073 | 0 | 0.0003 | 0.0011 | 0.0015 | 0.0004 | 0.003 | 0 | 0.0002 | 0 | 0.0008 | 0.0001 |
Nuclear Periphery | 0.0047 | 0.001 | 0.0126 | 0.0062 | 0.0174 | 0.0018 | 0.0148 | 0.0013 | 0.0022 | 0.0269 | 0.0158 | 0.0044 | 0.0152 | 0.0006 | 0.001 | 0.0003 | 0.0049 | 0.0009 |
Nucleolus | 0.032 | 0.0062 | 0.0221 | 0.0886 | 0.111 | 0.0492 | 0.0149 | 0.0071 | 0.0065 | 0.0182 | 0.0385 | 0.0316 | 0.0198 | 0.0056 | 0.0058 | 0.01 | 0.056 | 0.0228 |
Nucleus | 0.7776 | 0.9926 | 0.9392 | 0.7789 | 0.6294 | 0.9032 | 0.8992 | 0.9889 | 0.9668 | 0.8684 | 0.7823 | 0.9453 | 0.792 | 0.9935 | 0.9211 | 0.9894 | 0.6367 | 0.9402 |
Peroxisomes | 0.013 | 0 | 0.0045 | 0.0162 | 0.0014 | 0.0009 | 0.0022 | 0 | 0.0006 | 0.0008 | 0.0149 | 0.0001 | 0.0061 | 0 | 0.0092 | 0 | 0.0476 | 0.0011 |
Punctate Nuclear | 0.0184 | 0 | 0.0015 | 0.0052 | 0.001 | 0.0013 | 0.0166 | 0.0006 | 0.0082 | 0.0023 | 0.013 | 0.0022 | 0.0061 | 0 | 0.0006 | 0.0001 | 0.0039 | 0.0043 |
Vacuole | 0.0019 | 0 | 0.0028 | 0.0026 | 0.0086 | 0.0051 | 0.0019 | 0.0011 | 0.0004 | 0.003 | 0.001 | 0.0013 | 0.0041 | 0.0001 | 0.0038 | 0 | 0.0035 | 0.0007 |
Vacuole Periphery | 0.0016 | 0 | 0.0027 | 0.0008 | 0.0238 | 0.0019 | 0.0023 | 0.0004 | 0.0002 | 0.0042 | 0.0014 | 0.0005 | 0.0047 | 0.0002 | 0.0053 | 0 | 0.0028 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 73.8454 | 82.0706 | 100.239 | 67.6756 | 79.1088 | 59.2716 | 78.2139 | 65.9212 | 58.1071 | 71.3651 |
Translational Efficiency | 1.372 | 1.4187 | 1.0251 | 1.3245 | 1.0548 | 1.1687 | 1.2073 | 1.1928 | 1.1871 | 1.0308 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2413 | 1309 | 511 | 105 | 1640 | 1851 | 192 | 176 | 4053 | 3160 | 703 | 281 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 736.26 | 1037.44 | 1350.91 | 1632.22 | 969.01 | 1018.40 | 1606.44 | 1731.02 | 830.44 | 1026.29 | 1420.70 | 1694.10 |
Standard Deviation | 108.46 | 173.25 | 227.92 | 322.96 | 160.79 | 173.80 | 246.24 | 324.33 | 174.68 | 173.83 | 259.39 | 327.33 |
Intensity Change Log 2 | — | 0.494741 | 0.875644 | 1.148548 | — | 0.071721 | 0.729284 | 0.837039 | — | 0.269728 | 0.794305 | 0.979852 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000182 | 0.000355 | 0.000676 | 0.000997 | 0.000284 | 0.000459 | 0.000680 | 0.000798 | 0.000223 | 0.000416 | 0.000677 | 0.000872 |
Bud Neck | 0.003570 | 0.007354 | 0.010085 | 0.013835 | 0.003250 | 0.011827 | 0.005984 | 0.017487 | 0.003440 | 0.009974 | 0.008965 | 0.016123 |
Bud Site | 0.000936 | 0.000751 | 0.000769 | 0.002275 | 0.000293 | 0.001734 | 0.000438 | 0.003227 | 0.000676 | 0.001327 | 0.000679 | 0.002871 |
Cell Periphery | 0.000909 | 0.000119 | 0.000038 | 0.000053 | 0.000143 | 0.000350 | 0.000022 | 0.000065 | 0.000599 | 0.000254 | 0.000034 | 0.000061 |
Cytoplasm | 0.003835 | 0.000374 | 0.000157 | 0.000335 | 0.000153 | 0.000860 | 0.000044 | 0.000367 | 0.002345 | 0.000659 | 0.000126 | 0.000355 |
Cytoplasmic Foci | 0.000588 | 0.000253 | 0.000020 | 0.002107 | 0.000007 | 0.001261 | 0.000047 | 0.002779 | 0.000353 | 0.000844 | 0.000028 | 0.002528 |
Eisosomes | 0.000033 | 0.000043 | 0.000053 | 0.000074 | 0.000051 | 0.000064 | 0.000083 | 0.000058 | 0.000040 | 0.000056 | 0.000061 | 0.000064 |
Endoplasmic Reticulum | 0.008385 | 0.003711 | 0.001673 | 0.001179 | 0.004013 | 0.006677 | 0.001290 | 0.000582 | 0.006616 | 0.005448 | 0.001568 | 0.000805 |
Endosome | 0.000625 | 0.000236 | 0.000037 | 0.000211 | 0.000068 | 0.001111 | 0.000018 | 0.001089 | 0.000400 | 0.000749 | 0.000032 | 0.000761 |
Golgi | 0.000144 | 0.000191 | 0.000040 | 0.000291 | 0.000016 | 0.000839 | 0.000031 | 0.000445 | 0.000092 | 0.000571 | 0.000038 | 0.000387 |
Lipid Particles | 0.000125 | 0.000039 | 0.000027 | 0.000076 | 0.000009 | 0.000229 | 0.000017 | 0.000120 | 0.000078 | 0.000150 | 0.000025 | 0.000104 |
Mitochondria | 0.000489 | 0.000733 | 0.001205 | 0.002484 | 0.000360 | 0.001830 | 0.001347 | 0.002832 | 0.000437 | 0.001375 | 0.001244 | 0.002702 |
Mitotic Spindle | 0.000193 | 0.001144 | 0.000063 | 0.047120 | 0.000073 | 0.003878 | 0.000026 | 0.025704 | 0.000145 | 0.002745 | 0.000053 | 0.033707 |
None | 0.010478 | 0.002145 | 0.000689 | 0.001134 | 0.001079 | 0.001904 | 0.000334 | 0.002403 | 0.006674 | 0.002004 | 0.000592 | 0.001929 |
Nuclear Periphery | 0.000858 | 0.000166 | 0.000108 | 0.001773 | 0.000077 | 0.001110 | 0.000062 | 0.000275 | 0.000542 | 0.000719 | 0.000096 | 0.000835 |
Nuclear Periphery Foci | 0.000487 | 0.000055 | 0.000015 | 0.004153 | 0.000036 | 0.000531 | 0.000005 | 0.000500 | 0.000305 | 0.000334 | 0.000012 | 0.001865 |
Nucleolus | 0.008061 | 0.005785 | 0.006278 | 0.056949 | 0.006601 | 0.007298 | 0.011691 | 0.091408 | 0.007470 | 0.006671 | 0.007756 | 0.078532 |
Nucleus | 0.958452* | 0.975544* | 0.976952* | 0.847910* | 0.982860* | 0.952416* | 0.975961* | 0.837740* | 0.968328* | 0.961997* | 0.976681* | 0.841540* |
Peroxisomes | 0.000208 | 0.000288 | 0.000494 | 0.015819 | 0.000145 | 0.000992 | 0.001280 | 0.009367 | 0.000182 | 0.000700 | 0.000709 | 0.011778 |
Vacuole | 0.001153 | 0.000560 | 0.000546 | 0.001018 | 0.000408 | 0.003066 | 0.000572 | 0.002118 | 0.000852 | 0.002028 | 0.000553 | 0.001707 |
Vacuole Periphery | 0.000290 | 0.000153 | 0.000076 | 0.000207 | 0.000076 | 0.001562 | 0.000068 | 0.000635 | 0.000203 | 0.000978 | 0.000073 | 0.000475 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -7.30 | -13.02 | -9.55 | -8.04 | -4.52 | -5.66 | -9.01 | -6.68 | -5.26 | -3.27 | -9.31 | -15.18 | -10.59 | -8.44 | -4.88 |
Bud Neck | -4.51 | -9.84 | -4.78 | -3.80 | -3.01 | -8.58 | -5.90 | -6.41 | -3.91 | -5.47 | -9.37 | -11.46 | -7.97 | -5.44 | -5.63 |
Bud Site | 0.60 | 0.25 | -3.24 | -3.34 | -3.29 | -5.83 | -3.49 | -5.71 | -4.30 | -5.49 | -3.47 | -0.88 | -6.25 | -5.49 | -6.17 |
Cell Periphery | 4.60 | 5.09 | 4.74 | 0.91 | -4.48 | -2.19 | 4.59 | 2.09 | 2.88 | -5.85 | 2.98 | 5.50 | 4.96 | 3.00 | -6.27 |
Cytoplasm | 5.58 | 6.07 | 5.03 | -0.91 | -1.99 | -4.16 | 2.64 | -3.70 | 2.63 | -5.70 | 4.25 | 6.10 | 4.97 | 1.40 | -3.80 |
Cytoplasmic Foci | 1.32 | 2.89 | -2.43 | -2.68 | -2.84 | -3.09 | -2.23 | -2.85 | -2.12 | -2.81 | -1.46 | 2.74 | -3.56 | -3.19 | -3.87 |
Eisosomes | -2.63 | -6.76 | -6.65 | -5.42 | -4.23 | -2.21 | -5.99 | -3.71 | -1.76 | 0.79 | -4.05 | -7.84 | -7.77 | -4.69 | -3.64 |
Endoplasmic Reticulum | 8.76 | 13.22 | 10.72 | 4.22 | 0.22 | -6.60 | 10.55 | 14.02 | 16.12 | 2.70 | 3.07 | 15.55 | 16.14 | 15.23 | 2.74 |
Endosome | 1.79 | 3.00 | 1.26 | -0.77 | -2.97 | -4.55 | 6.25 | -1.19 | 0.85 | -1.28 | -1.99 | 3.13 | -0.43 | 0.63 | -1.60 |
Golgi | -0.36 | 1.58 | -4.20 | -2.59 | -5.26 | -2.24 | -3.52 | -5.45 | 0.03 | -5.27 | -2.12 | 1.28 | -6.38 | -0.94 | -7.17 |
Lipid Particles | 2.05 | 2.66 | -1.62 | -2.85 | -3.11 | -1.95 | -2.29 | -2.96 | 0.40 | -2.80 | -1.22 | 2.24 | -2.75 | -0.48 | -3.82 |
Mitochondria | -1.30 | -9.22 | -7.02 | -5.57 | -4.58 | -5.14 | -12.70 | -4.20 | -1.69 | -2.54 | -5.04 | -13.99 | -6.03 | -3.43 | -3.89 |
Mitotic Spindle | -1.27 | 1.07 | -3.96 | -3.91 | -3.96 | -3.82 | 0.27 | -4.57 | -4.25 | -4.57 | -3.93 | 0.98 | -6.04 | -5.79 | -6.05 |
None | 5.44 | 7.92 | 6.67 | 0.53 | -1.60 | -1.71 | 3.49 | -2.86 | -0.77 | -4.68 | 5.33 | 8.14 | 5.65 | -0.15 | -4.32 |
Nuclear Periphery | 2.74 | 2.91 | -0.66 | -1.75 | -1.81 | -3.11 | 0.60 | -4.26 | 1.95 | -4.33 | -0.75 | 2.87 | -0.78 | -0.14 | -2.61 |
Nuclear Periphery Foci | 2.26 | 2.45 | -1.11 | -1.40 | -1.42 | -3.03 | 1.28 | -1.80 | -0.68 | -1.88 | -0.06 | 2.53 | -1.59 | -1.59 | -2.07 |
Nucleolus | 2.14 | 1.39 | -5.72 | -5.81 | -5.77 | -0.90 | -2.02 | -8.61 | -8.56 | -7.88 | 0.99 | -1.36 | -10.30 | -10.35 | -10.13 |
Nucleus | -5.21 | -6.41 | 6.91 | 7.38 | 7.37 | 9.15 | 2.39 | 10.55 | 9.31 | 9.91 | 2.71 | -2.40 | 12.31 | 11.95 | 12.54 |
Peroxisomes | -0.78 | -3.48 | -3.75 | -3.73 | -3.69 | -2.02 | -3.92 | -4.64 | -4.38 | -4.30 | -2.08 | -5.44 | -5.97 | -5.76 | -5.78 |
Vacuole | 3.17 | 2.36 | -1.31 | -1.45 | -1.42 | -4.58 | -2.43 | -2.74 | 0.46 | -2.45 | -3.36 | 1.35 | -2.01 | -1.23 | -2.12 |
Vacuole Periphery | 2.40 | 4.45 | -1.13 | -3.36 | -5.12 | -4.40 | -0.16 | -2.36 | 2.28 | -2.36 | -3.86 | 4.35 | -2.19 | 1.81 | -3.05 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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