Standard name | |
---|---|
Human Ortholog | |
Description | Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 2 | 1 | 0 | 15 | 7 | 8 | 9 | 12 | 7 | 21 | 23 | 21 | 40 | 0 | 0 | 0 | 0 | 2 | 7 | 4 | 8 | 9 |
Bud | 0 | 1 | 2 | 2 | 0 | 2 | 9 | 10 | 9 | 5 | 11 | 17 | 15 | 15 | 14 | 0 | 1 | 0 | 0 | 1 | 4 | 2 | 7 | 12 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 53 | 59 | 168 | 125 | 121 | 152 | 149 | 152 | 188 | 159 | 151 | 263 | 535 | 535 | 511 | 29 | 51 | 10 | 56 | 57 | 235 | 171 | 316 | 320 |
Cytoplasm | 13 | 25 | 21 | 18 | 28 | 33 | 42 | 65 | 81 | 69 | 76 | 78 | 20 | 20 | 51 | 27 | 106 | 32 | 18 | 46 | 8 | 26 | 24 | 17 |
Endoplasmic Reticulum | 5 | 1 | 11 | 12 | 59 | 57 | 102 | 139 | 80 | 101 | 55 | 81 | 193 | 280 | 227 | 0 | 10 | 0 | 33 | 19 | 54 | 52 | 153 | 238 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 2 | 1 | 0 | 2 | 21 | 12 | 41 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 2 | 30 | 34 | 67 | 95 |
Nucleus | 0 | 0 | 0 | 1 | 1 | 0 | 4 | 6 | 3 | 5 | 2 | 9 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 3 | 3 | 3 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 1 | 0 | 2 | 6 | 6 | 5 | 5 | 3 | 11 | 3 | 3 | 5 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 2 |
Unique Cell Count | 63 | 68 | 195 | 154 | 196 | 229 | 286 | 354 | 325 | 309 | 249 | 401 | 732 | 798 | 795 | 53 | 144 | 40 | 113 | 134 | 346 | 298 | 586 | 703 |
Labelled Cell Count | 71 | 86 | 204 | 162 | 209 | 261 | 320 | 390 | 382 | 361 | 310 | 485 | 811 | 887 | 894 | 57 | 171 | 43 | 113 | 134 | 346 | 298 | 586 | 703 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 138.8 | 122.7 | 118.1 | 119.6 | 120.3 | 96.2 | 87.7 | 83.7 | 80.9 | 76.8 | 74.3 | 75.6 | 80.2 | 80.2 | 58.8 | 85.5 | 94.5 | 119.1 | 125.5 | 116.7 | 118.5 |
Std Deviation (1e-4) | 23.6 | 24.8 | 29.4 | 34.8 | 33.3 | 32.9 | 30.8 | 28.2 | 28.0 | 25.2 | 26.4 | 22.5 | 16.3 | 16.3 | 14.6 | 23.3 | 30.1 | 39.7 | 29.0 | 30.7 | 27.9 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.03 | -0.3 | -0.43 | -0.5 | -0.55 | -0.62 | -0.67 | -0.64 | -0.56 | -0.56 | -1.01 | -0.47 | -0.32 | 0.01 | 0.09 | -0.02 | 0.01 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 2.9 | 0 | 0 | 0 | 1.9 | 0 | 2.5 | 1.6 | 0 | 2.5 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.1 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -1.3 | -5.5 | -4.7 | -7.7 | -9.8 | -6.7 | -7.9 | -5.9 | -5.3 | -3.8 | -5.3 | -5.9 | -5.0 | -9.7 | -8.2 |
Cytoplasm | 0.3 | 1.1 | 1.1 | 1.2 | 2.3 | 3.9 | 3.3 | 5.0 | 2.7 | -4.9 | -5.2 | -2.1 | 6.6 | 11.8 | 9.5 |
Endoplasmic Reticulum | 0.8 | 6.3 | 5.4 | 7.6 | 8.5 | 5.5 | 7.1 | 4.8 | 4.6 | 6.2 | 8.1 | 6.7 | 0 | 0.5 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 80.5676 | 131.085 | 118.14 | 109.8896 | 122.3924 | 117.49 | 93.0527 | 139.9834 | 128.8896 | 123.0101 | 90.6754 | 130.7585 | 94.791 | 146.5711 | 133.5515 | 113.6739 | 87.7028 | 132.6287 |
Actin | 0.0305 | 0 | 0.0007 | 0.0103 | 0 | 0.0015 | 0.0145 | 0.0003 | 0.0058 | 0.0056 | 0.0002 | 0 | 0.0222 | 0 | 0.0001 | 0.0009 | 0.0006 | 0.0001 |
Bud | 0.0014 | 0 | 0.0001 | 0.0002 | 0 | 0.0002 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.001 | 0.0001 | 0.0009 | 0.0001 | 0.0003 | 0.0002 | 0.0006 | 0.0001 |
Bud Neck | 0.0016 | 0.0001 | 0.0005 | 0.0009 | 0.0001 | 0.0003 | 0.0007 | 0.0005 | 0.0018 | 0.0011 | 0.0009 | 0.0004 | 0.006 | 0.0003 | 0.0013 | 0.001 | 0.0007 | 0.0011 |
Bud Periphery | 0.0021 | 0.0002 | 0.0005 | 0.0013 | 0.0003 | 0.0012 | 0.003 | 0.0005 | 0.0011 | 0.0009 | 0.0027 | 0.0011 | 0.0029 | 0.0004 | 0.0017 | 0.0025 | 0.0018 | 0.0013 |
Bud Site | 0.0127 | 0.0004 | 0.0004 | 0.0005 | 0 | 0.0001 | 0.0097 | 0.0043 | 0.0024 | 0.0002 | 0.0033 | 0 | 0.0053 | 0.0078 | 0.003 | 0.0002 | 0.0038 | 0.0003 |
Cell Periphery | 0.5371 | 0.6531 | 0.6674 | 0.6835 | 0.7291 | 0.6354 | 0.692 | 0.7614 | 0.7513 | 0.7438 | 0.5934 | 0.7083 | 0.7119 | 0.8006 | 0.8101 | 0.7336 | 0.6253 | 0.7069 |
Cytoplasm | 0.0086 | 0.0037 | 0.0025 | 0.0042 | 0.0009 | 0.0007 | 0.0012 | 0.0008 | 0.0018 | 0.0002 | 0.0011 | 0.0001 | 0.0012 | 0.0009 | 0.0024 | 0.0046 | 0.0004 | 0.0008 |
Cytoplasmic Foci | 0.0185 | 0 | 0.0109 | 0.0024 | 0 | 0.0003 | 0.0123 | 0.0001 | 0.0015 | 0.0119 | 0.0926 | 0 | 0.0079 | 0 | 0.0003 | 0.0004 | 0.0008 | 0.023 |
Eisosomes | 0.0032 | 0.0005 | 0.0011 | 0.001 | 0.0004 | 0.0005 | 0.0095 | 0.006 | 0.0125 | 0.0026 | 0.0008 | 0.0002 | 0.0052 | 0.0093 | 0.0049 | 0.0027 | 0.0307 | 0.0082 |
Endoplasmic Reticulum | 0.0059 | 0.0031 | 0.0063 | 0.0133 | 0.0042 | 0.0048 | 0.0037 | 0.0017 | 0.0036 | 0.0041 | 0.0053 | 0.0011 | 0.003 | 0.001 | 0.0019 | 0.0054 | 0.0023 | 0.0013 |
Endosome | 0.0067 | 0 | 0.0012 | 0.0036 | 0 | 0.0073 | 0.0064 | 0.0017 | 0.0016 | 0.0005 | 0.0003 | 0 | 0.0005 | 0 | 0.0002 | 0.0008 | 0.0001 | 0.0001 |
Golgi | 0.0045 | 0 | 0.0003 | 0.0011 | 0 | 0.0014 | 0.0084 | 0.0003 | 0.0006 | 0.0018 | 0.0002 | 0 | 0.001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 |
Lipid Particles | 0.0105 | 0.0048 | 0.0099 | 0.0038 | 0.0021 | 0.0053 | 0.0064 | 0.0045 | 0.0076 | 0.0037 | 0.0163 | 0.0008 | 0.0039 | 0.0017 | 0.0012 | 0.0014 | 0.0028 | 0.005 |
Mitochondria | 0.0058 | 0 | 0.0006 | 0.0008 | 0 | 0.0017 | 0.0107 | 0.0001 | 0.0002 | 0.0003 | 0.0084 | 0 | 0.0057 | 0.0001 | 0.0016 | 0.0003 | 0.156 | 0.0075 |
None | 0.0178 | 0 | 0.0009 | 0.0018 | 0 | 0.0005 | 0.0032 | 0 | 0.0009 | 0.0001 | 0.0054 | 0 | 0.0021 | 0 | 0.0038 | 0.0443 | 0.0081 | 0.0077 |
Nuclear Periphery | 0.0092 | 0.0144 | 0.0207 | 0.0389 | 0.0088 | 0.0236 | 0.016 | 0.0132 | 0.0206 | 0.0173 | 0.0164 | 0.0059 | 0.0148 | 0.0125 | 0.013 | 0.0157 | 0.0107 | 0.0073 |
Nucleolus | 0.0187 | 0.0004 | 0.0008 | 0.0008 | 0.0002 | 0.0011 | 0.0081 | 0.0008 | 0.0012 | 0.0012 | 0.0905 | 0.0007 | 0.0086 | 0.001 | 0.0018 | 0.0031 | 0.0187 | 0.0144 |
Nucleus | 0.0093 | 0.0003 | 0.0045 | 0.0023 | 0.0003 | 0.0013 | 0.0011 | 0.0007 | 0.0009 | 0.0008 | 0.0144 | 0.0006 | 0.0019 | 0.0009 | 0.0014 | 0.0071 | 0.003 | 0.0017 |
Peroxisomes | 0.0113 | 0 | 0.0005 | 0.0002 | 0 | 0.0002 | 0.0025 | 0 | 0.0004 | 0.0268 | 0.0022 | 0 | 0.0007 | 0 | 0.0001 | 0.0001 | 0.003 | 0.0009 |
Punctate Nuclear | 0.0115 | 0 | 0.0014 | 0.0039 | 0 | 0.0009 | 0.0005 | 0 | 0.0001 | 0.0003 | 0.0017 | 0 | 0.0015 | 0 | 0 | 0.0085 | 0 | 0.0005 |
Vacuole | 0.2695 | 0.318 | 0.2676 | 0.2217 | 0.2526 | 0.3086 | 0.1863 | 0.2014 | 0.1818 | 0.1739 | 0.1385 | 0.2771 | 0.1902 | 0.1623 | 0.1477 | 0.1628 | 0.1262 | 0.2084 |
Vacuole Periphery | 0.0036 | 0.001 | 0.0014 | 0.0033 | 0.0012 | 0.003 | 0.003 | 0.0015 | 0.0022 | 0.0028 | 0.0046 | 0.0035 | 0.0028 | 0.0008 | 0.0032 | 0.0042 | 0.0041 | 0.0035 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 139.8197 | 107.1345 | 139.8076 | 173.7889 | 147.7942 | 99.1275 | 122.7978 | 116.8097 | 150.3507 | 165.3681 |
Translational Efficiency | 1.3335 | 1.339 | 1.2966 | 1.1141 | 1.1984 | 1.4782 | 1.1498 | 1.0293 | 1.019 | 1.0281 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
100 | 234 | 902 | 418 | 1674 | 588 | 1054 | 615 | 1774 | 822 | 1956 | 1033 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1711.57 | 8335.32 | 1958.86 | 1657.25 | 7061.02 | 7253.51 | 2607.12 | 1687.87 | 6759.47 | 7561.47 | 2308.18 | 1675.48 |
Standard Deviation | 376.18 | 1635.42 | 406.11 | 324.55 | 2036.57 | 1641.67 | 664.98 | 458.45 | 2333.23 | 1711.01 | 647.11 | 409.85 |
Intensity Change Log 2 | — | 2.283917 | 0.194694 | -0.046529 | — | 0.038803 | -1.437420 | -2.064673 | — | 0.829438 | -0.942078 | -1.390945 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000216 | 0.000273 | 0.001190 | 0.001789 | 0.000338 | 0.000296 | 0.012853 | 0.001175 | 0.000331 | 0.000289 | 0.007475 | 0.001423 |
Bud Neck | 0.004797 | 0.000710 | 0.005901 | 0.005476 | 0.001169 | 0.000885 | 0.007679 | 0.005347 | 0.001373 | 0.000835 | 0.006859 | 0.005399 |
Bud Site | 0.019870 | 0.003577 | 0.006660 | 0.016151 | 0.005502 | 0.001522 | 0.019934 | 0.023376 | 0.006312 | 0.002107 | 0.013812 | 0.020452 |
Cell Periphery | 0.009347 | 0.536844* | 0.002249 | 0.007953 | 0.459991* | 0.602810* | 0.010663 | 0.004330 | 0.434588* | 0.584031* | 0.006783 | 0.005796 |
Cytoplasm | 0.015899 | 0.000171 | 0.000785 | 0.002894 | 0.020591 | 0.000319 | 0.002002 | 0.006624 | 0.020327 | 0.000277 | 0.001441 | 0.005115 |
Cytoplasmic Foci | 0.011290 | 0.000017 | 0.013978 | 0.011917 | 0.000136 | 0.000073 | 0.006969 | 0.033575 | 0.000764 | 0.000057 | 0.010201 | 0.024811 |
Eisosomes | 0.000035 | 0.006039 | 0.000123 | 0.000047 | 0.001029 | 0.003124 | 0.000701 | 0.000037 | 0.000973 | 0.003954 | 0.000435 | 0.000041 |
Endoplasmic Reticulum | 0.017478 | 0.001308 | 0.001310 | 0.038350 | 0.001349 | 0.002591 | 0.016330 | 0.008554 | 0.002259 | 0.002226 | 0.009404 | 0.020611 |
Endosome | 0.027159 | 0.000129 | 0.083338 | 0.089083 | 0.000205 | 0.000254 | 0.180773 | 0.158240 | 0.001724 | 0.000218 | 0.135841 | 0.130256 |
Golgi | 0.028042 | 0.000384 | 0.077728 | 0.049370 | 0.001135 | 0.001616 | 0.123820* | 0.094676 | 0.002652 | 0.001265 | 0.102565 | 0.076343 |
Lipid Particles | 0.007590 | 0.000978 | 0.090188 | 0.045138 | 0.005010 | 0.001031 | 0.238405* | 0.055696 | 0.005155 | 0.001016 | 0.170056* | 0.051424 |
Mitochondria | 0.000170 | 0.000051 | 0.003935 | 0.000567 | 0.000150 | 0.000642 | 0.004847 | 0.004943 | 0.000151 | 0.000473 | 0.004426 | 0.003172 |
Mitotic Spindle | 0.001002 | 0.000579 | 0.000276 | 0.001455 | 0.000415 | 0.000578 | 0.000654 | 0.010234 | 0.000448 | 0.000579 | 0.000480 | 0.006682 |
None | 0.000027 | 0.000004 | 0.000125 | 0.000041 | 0.000111 | 0.000008 | 0.000198 | 0.000221 | 0.000106 | 0.000007 | 0.000165 | 0.000148 |
Nuclear Periphery | 0.000893 | 0.003569 | 0.002997 | 0.001291 | 0.006473 | 0.006180 | 0.000899 | 0.000848 | 0.006159 | 0.005437 | 0.001866 | 0.001027 |
Nuclear Periphery Foci | 0.004656 | 0.000202 | 0.011334 | 0.018130 | 0.000799 | 0.000213 | 0.003539 | 0.010348 | 0.001016 | 0.000210 | 0.007134 | 0.013497 |
Nucleolus | 0.001278 | 0.000047 | 0.001126 | 0.000131 | 0.000103 | 0.000065 | 0.000202 | 0.001226 | 0.000169 | 0.000060 | 0.000629 | 0.000783 |
Nucleus | 0.001305 | 0.000060 | 0.000211 | 0.000543 | 0.000359 | 0.000086 | 0.000352 | 0.000907 | 0.000413 | 0.000079 | 0.000287 | 0.000760 |
Peroxisomes | 0.000252 | 0.000028 | 0.000456 | 0.000170 | 0.000117 | 0.000035 | 0.000591 | 0.000693 | 0.000125 | 0.000033 | 0.000529 | 0.000481 |
Vacuole | 0.786356* | 0.442470* | 0.576005* | 0.671123* | 0.490311* | 0.374441* | 0.320680* | 0.525892* | 0.506999* | 0.393807* | 0.438422* | 0.584659* |
Vacuole Periphery | 0.062336 | 0.002557 | 0.120085* | 0.038381 | 0.004707 | 0.003231 | 0.047907 | 0.053058 | 0.007956 | 0.003039 | 0.081191 | 0.047119 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.11 | -2.58 | -1.94 | -1.85 | -0.68 | 0.40 | -6.60 | -1.77 | -1.88 | 5.97 | 0.45 | -6.82 | -2.50 | -2.63 | 5.35 |
Bud Neck | 2.96 | -0.63 | -0.40 | -5.61 | 0.32 | 0.61 | -10.70 | -6.47 | -6.81 | 2.85 | 1.42 | -10.32 | -7.28 | -8.64 | 2.13 |
Bud Site | 2.34 | 2.10 | 0.80 | -2.94 | -3.12 | 3.28 | -5.57 | -5.47 | -6.79 | -0.56 | 2.95 | -4.42 | -5.84 | -7.57 | -2.23 |
Cell Periphery | -32.83* | 2.71 | 0.40 | 33.24* | -6.67 | -11.31 | 65.88* | 67.29* | 55.41* | 4.19 | -13.32 | 62.63 | 62.81 | 64.20* | 1.13 |
Cytoplasm | 3.02 | 2.92 | 2.59 | -4.05 | -2.78 | 8.75 | 7.32 | 4.70 | -5.42 | -3.25 | 9.19 | 8.30 | 6.13 | -6.46 | -4.17 |
Cytoplasmic Foci | 3.05 | -0.13 | 0.33 | -5.13 | 0.80 | 1.33 | -9.20 | -10.20 | -10.22 | -7.85 | 3.19 | -10.67 | -10.85 | -11.30 | -6.19 |
Eisosomes | -3.26 | -4.66 | -0.89 | 3.25 | 4.64 | -2.24 | 1.18 | 3.75 | 3.44 | 7.19 | -3.44 | 2.12 | 3.73 | 4.72 | 7.77 |
Endoplasmic Reticulum | 2.91 | 2.91 | -2.70 | -6.19 | -6.20 | -1.24 | -8.14 | -4.18 | -2.99 | 3.08 | -0.01 | -6.78 | -6.85 | -6.66 | -3.96 |
Endosome | 7.45 | -9.68 | -8.07 | -13.30 | -0.66 | -0.53 | -24.00 | -21.36 | -21.35 | 2.15 | 5.72 | -28.65 | -24.35 | -24.67 | 0.84 |
Golgi | 3.88 | -6.18 | -2.42 | -8.17 | 3.86 | -0.45 | -22.44 | -14.49 | -14.22 | 3.62 | 1.54 | -27.18 | -15.97 | -16.12 | 4.68 |
Lipid Particles | 2.22 | -11.73 | -5.26 | -6.74 | 4.89 | 4.57 | -25.65 | -8.89 | -9.67 | 17.11 | 5.11 | -27.49 | -10.64 | -11.76 | 16.17 |
Mitochondria | 1.89 | -5.97 | -1.35 | -1.96 | 4.99 | -0.92 | -5.73 | -2.23 | -1.94 | 0.04 | -0.82 | -8.09 | -2.35 | -2.01 | 1.01 |
Mitotic Spindle | 0.77 | 0.99 | 0.08 | -1.77 | -2.33 | -3.95 | -1.01 | -3.83 | -3.78 | -3.63 | -1.16 | -0.46 | -4.00 | -3.95 | -3.90 |
None | 3.08 | -5.50 | -0.97 | -3.15 | 4.08 | 4.43 | -1.80 | -1.15 | -2.17 | -0.04 | 4.55 | -1.86 | -0.77 | -2.41 | 0.35 |
Nuclear Periphery | -4.82 | -3.54 | -0.84 | 4.27 | 2.97 | 0.40 | 17.65 | 16.77 | 8.30 | 0.27 | 1.30 | 11.57 | 15.48 | 8.97 | 3.08 |
Nuclear Periphery Foci | 3.66 | -2.84 | -3.60 | -5.24 | -1.89 | 6.78 | -10.53 | -7.36 | -7.80 | -5.24 | 7.42 | -7.51 | -7.86 | -8.39 | -3.76 |
Nucleolus | 1.21 | 0.11 | 1.13 | -5.01 | 4.41 | 3.50 | -5.66 | -1.66 | -1.71 | -1.50 | 1.91 | -3.93 | -1.51 | -1.79 | -0.35 |
Nucleus | 2.10 | 1.90 | 1.24 | -1.96 | -1.43 | 5.97 | -0.64 | -1.67 | -2.47 | -1.14 | 5.90 | 0.57 | -1.57 | -3.10 | -1.75 |
Peroxisomes | 2.00 | -1.20 | 0.80 | -3.48 | 2.74 | 4.86 | -2.45 | -5.50 | -6.32 | -0.53 | 5.51 | -3.55 | -5.38 | -6.92 | 0.32 |
Vacuole | 12.03 | 8.30 | 3.91 | -10.13 | -5.29 | 9.09 | 14.39 | -2.47 | -8.99 | -12.67 | 9.98 | 6.84 | -6.43 | -13.99 | -11.67 |
Vacuole Periphery | 5.74 | -5.59 | 2.10 | -13.63 | 16.87 | 4.14 | -19.73 | -14.21 | -14.69 | -1.10 | 6.70 | -29.70 | -16.41 | -19.27 | 10.63 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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