Standard name
Human Ortholog
Description Cobalamin-independent methionine synthase; involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.1 0.18 0 0 0 0 0.05 0.06 0.07 0 0 0.08 0.1 0.29 0.24 0.44 0.57 0.36 0 0 0 0 0 0
Cytoplasm 0.97 0.88 1.0 1.0 0.98 0.98 0.97 0.96 0.92 0.94 0.94 0.91 0.92 0.81 0.8 0.73 0.61 0.77 0.96 0.96 0.98 0.95 0.91 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1 0 0 0 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 18 47 3 0 3 4 6 9 8 5 5 15 14 33 35 92 88 50 2 4 0 0 0 2
Cytoplasm 167 232 107 88 100 124 120 153 101 116 133 162 123 92 114 154 95 106 197 326 114 119 190 179
Endoplasmic Reticulum 0 1 0 0 0 0 2 2 5 4 5 7 0 0 0 1 6 7 0 0 0 1 4 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 1 0 0 3 1
Unique Cell Count 173 265 107 88 102 127 124 160 110 123 142 178 134 114 143 210 155 137 206 339 117 126 208 191
Labelled Cell Count 185 281 110 88 103 129 129 164 116 127 144 186 137 125 149 247 190 163 206 339 117 126 208 191


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 456.2 470.6 330.6 291.3 284.5 227.7 206.3 175.4 158.2 128.5 130.5 135.3 485.3 505.9 487.9 627.9 637.1 589.7 375.9 402.8 423.1
Std Deviation (1e-4) 91.5 88.5 80.2 94.2 77.9 72.6 63.4 60.6 50.9 47.0 42.0 39.5 86.4 132.5 108.3 119.5 121.8 127.3 99.2 113.1 134.5
Intensity Change (Log2) – – – -0.18 -0.22 -0.54 -0.68 -0.91 -1.06 -1.36 -1.34 -1.29 0.55 0.61 0.56 0.93 0.95 0.83 0.19 0.29 0.36

WT3RAP60RAP140RAP220RAP300RAP380RAP460RAP540RAP620RAP7000200400600WT3HU80HU120HU1600200400600WT3rpd3Δ_1rpd3Δ_2rpd3Δ_30200400600WT1AF100AF140AF1800200400600
rapamycinhydroxy-urearpd3 knockoutalpha-factorMultiple Condition Time-Course Intensity PlotsGFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)GFP Intensity (1e-4)

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 1.5 0 0 1.9 2.3 5.3 4.7 7.5 9.0 6.3
Cytoplasm 0 -1.5 -1.6 -1.9 -2.2 -3.0 -2.5 -2.7 -3.2 -3.0 -4.8 -5.0 -5.9 -7.3 -5.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 530.3302 807.6401 594.5139 550.3847 618.4806 652.8472 445.16 984.2512 563.7692 401.5141 447.7044 736.0263 688.4178 987.9898 729.2842 807.5749 670.1007 756.4286
Actin 0.013 0.0002 0.0091 0.0166 0.0003 0.0006 0.0017 0.0002 0.004 0.0016 0.0456 0.0009 0.0008 0.0002 0.0004 0.0002 0.0004 0.0008
Bud 0.0008 0.0008 0.0049 0.0004 0.0002 0.0008 0.0036 0.0002 0.0031 0.0053 0.0394 0.0004 0.0042 0.0009 0.0026 0.0004 0.0008 0.001
Bud Neck 0.0006 0.0002 0.0013 0.0006 0.0016 0.0008 0.0008 0.0001 0.0005 0.0011 0.0007 0.0008 0.0004 0.0001 0.0003 0.0003 0.001 0.0006
Bud Periphery 0.0005 0.0002 0.003 0.0002 0 0.0001 0.0018 0 0.0044 0.0021 0.0289 0.0001 0.0017 0.0002 0.001 0.0002 0.0002 0.0002
Bud Site 0.0007 0.0006 0.0029 0.002 0 0.0001 0.0043 0.0001 0.0016 0.0063 0.0182 0 0.0011 0.0001 0.0005 0.0002 0.0002 0.0001
Cell Periphery 0.0042 0.0033 0.0017 0.0001 0.0005 0.0004 0.0062 0.0003 0.004 0.0125 0.0037 0.0003 0.0029 0.0005 0.0014 0.0007 0.0024 0.0004
Cytoplasm 0.7424 0.9424 0.8098 0.7246 0.9732 0.8615 0.5912 0.98 0.6954 0.6543 0.5514 0.9397 0.8279 0.9706 0.8337 0.8742 0.8069 0.9559
Cytoplasmic Foci 0.0302 0.0001 0.0043 0.0158 0.0002 0.0007 0.0328 0.0002 0.0148 0.0517 0.0201 0.0006 0.0219 0.0001 0.0143 0.0038 0.0307 0.001
Eisosomes 0.0017 0 0.0011 0.0001 0 0.0003 0.0008 0 0.0053 0.0054 0.0035 0.0001 0.0009 0 0.0006 0.0006 0.0002 0.0001
Endoplasmic Reticulum 0.0072 0.0001 0.0045 0.0001 0.0002 0.0001 0.0022 0 0.0021 0.0015 0.0017 0.0001 0.0009 0.0001 0.0005 0.0002 0.0005 0.0003
Endosome 0.0063 0 0.0161 0.004 0 0.0001 0.0116 0 0.005 0.0002 0.0007 0.0002 0.0054 0 0.0048 0 0.0001 0.0001
Golgi 0.0055 0 0.0033 0.0082 0 0 0.0008 0 0.001 0.0002 0.0007 0 0.0006 0 0.0002 0 0 0
Lipid Particles 0.0059 0 0.0046 0.0274 0 0.0003 0.006 0 0.0052 0.0056 0.0013 0 0.0025 0 0.001 0.0003 0.0013 0.0002
Mitochondria 0.0155 0.0024 0.0114 0.0053 0.0003 0.0032 0.0284 0.0014 0.0515 0.0308 0.2104 0.0034 0.0221 0.001 0.014 0.0117 0.0081 0.0016
None 0.0192 0.0006 0.0229 0.0004 0.0002 0.0029 0.0288 0.0006 0.0215 0.1255 0.0376 0.0023 0.0205 0.0007 0.0311 0.0609 0.0131 0.0017
Nuclear Periphery 0.0136 0.0025 0.0141 0.0052 0.006 0.0098 0.0159 0.002 0.0132 0.0041 0.0014 0.0079 0.0086 0.0019 0.0041 0.0035 0.0094 0.0064
Nucleolus 0.0507 0.0032 0.0089 0.0002 0.0002 0.0302 0.111 0.0005 0.0493 0.0382 0.0053 0.0038 0.0283 0.0005 0.0489 0.0101 0.0183 0.0013
Nucleus 0.0656 0.0394 0.0536 0.0683 0.0161 0.0823 0.0904 0.0138 0.0362 0.0297 0.0126 0.036 0.0388 0.0213 0.0276 0.0299 0.0979 0.0261
Peroxisomes 0.0019 0.0001 0.0012 0.119 0 0.0001 0.0015 0 0.0015 0.0022 0.0092 0.0001 0.0011 0 0.0006 0.0003 0.0005 0.0001
Punctate Nuclear 0.007 0 0.0024 0.001 0.0001 0.0018 0.0079 0.0001 0.0368 0.0034 0.0007 0.0005 0.0042 0.0001 0.0077 0.0009 0.0023 0.0004
Vacuole 0.0052 0.0032 0.011 0.0004 0.0007 0.0018 0.0415 0.0002 0.0238 0.0154 0.0045 0.0012 0.0036 0.0013 0.0031 0.001 0.0039 0.0011
Vacuole Periphery 0.0024 0.0007 0.0079 0.0004 0.0004 0.0021 0.0107 0.0002 0.0198 0.0028 0.0023 0.0015 0.0015 0.0004 0.0017 0.0004 0.0017 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 493.036 221.7634 228.0939 405.3018 268.5502 339.3579 119.2547 144.9092 301.8031 252.1417
Translational Efficiency 1.5684 1.4905 1.4591 1.4629 1.6271 1.762 1.4347 1.0996 1.186 1.2546

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1784 304 214 1110 1193 2095 780 1112 2977 2399 994 2222

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 20969.91 30417.35 20682.70 13622.37 21610.04 28272.52 15687.99 13644.51 21226.44 28544.31 16763.31 13633.45
Standard Deviation 6303.76 6860.87 6745.75 4697.34 5844.80 7153.12 4042.42 4536.71 6132.00 7152.43 5180.20 4617.66
Intensity Change Log 2 — 0.536574 -0.019896 -0.622343 — 0.387699 -0.462041 -0.663381 — 0.462937 -0.227398 -0.643025

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000024 0.000122 0.000202 0.001432 0.000063 0.000052 0.000106 0.000200 0.000040 0.000061 0.000126 0.000816
Bud Neck 0.000095 0.001561 0.008134 0.001926 0.000544 0.000222 0.001836 0.001805 0.000275 0.000392 0.003192 0.001865
Bud Site 0.001522 0.003575 0.002272 0.010565 0.001518 0.001048 0.004012 0.005569 0.001520 0.001368 0.003638 0.008065
Cell Periphery 0.003647 0.028891 0.032832 0.016169 0.008191 0.003374 0.008702 0.009744 0.005468 0.006607 0.013897 0.012954
Cytoplasm 0.926578 0.694489 0.447661 0.779068 0.834296 0.908760 0.778538 0.803216 0.889597 0.881608 0.707303 0.791153
Cytoplasmic Foci 0.002743 0.024502 0.056449 0.010362 0.009184 0.003760 0.011725 0.013207 0.005324 0.006388 0.021354 0.011786
Eisosomes 0.000000 0.000006 0.000017 0.000006 0.000002 0.000001 0.000003 0.000004 0.000001 0.000001 0.000006 0.000005
Endoplasmic Reticulum 0.000028 0.000120 0.000048 0.000048 0.000042 0.000060 0.000028 0.000053 0.000034 0.000067 0.000032 0.000051
Endosome 0.000041 0.000277 0.000274 0.001428 0.000090 0.000061 0.000159 0.000567 0.000061 0.000088 0.000184 0.000997
Golgi 0.002712 0.019957 0.012585 0.001385 0.009369 0.002744 0.002307 0.002178 0.005380 0.004925 0.004520 0.001782
Lipid Particles 0.000667 0.012933 0.012025 0.010719 0.003597 0.001364 0.008382 0.007323 0.001841 0.002830 0.009166 0.009019
Mitochondria 0.000116 0.000249 0.000320 0.000415 0.000164 0.000133 0.000104 0.000488 0.000135 0.000148 0.000151 0.000452
Mitotic Spindle 0.000152 0.000965 0.001180 0.000554 0.000699 0.000244 0.000532 0.001449 0.000371 0.000336 0.000672 0.001002
None 0.008536 0.005483 0.002379 0.005854 0.009369 0.007886 0.003321 0.005708 0.008870 0.007581 0.003118 0.005781
Nuclear Periphery 0.000066 0.002381 0.004926 0.000234 0.000494 0.000138 0.000405 0.000286 0.000238 0.000422 0.001378 0.000260
Nuclear Periphery Foci 0.000645 0.005628 0.004681 0.001466 0.002850 0.001247 0.002236 0.001988 0.001529 0.001802 0.002763 0.001727
Nucleolus 0.000040 0.000152 0.000268 0.000326 0.000084 0.000070 0.000111 0.000159 0.000058 0.000081 0.000145 0.000243
Nucleus 0.038733 0.093775 0.219102 0.063445 0.078784 0.051942 0.086827 0.082167 0.054783 0.057243 0.115304 0.072814
Peroxisomes 0.000011 0.000014 0.000019 0.000243 0.000010 0.000012 0.000027 0.000057 0.000010 0.000012 0.000025 0.000150
Vacuole 0.008696 0.080619 0.179272 0.092336 0.027435 0.014428 0.088168 0.061874 0.016206 0.022816 0.107782 0.077091
Vacuole Periphery 0.004945 0.024303 0.015353 0.002018 0.013215 0.002455 0.002472 0.001959 0.008259 0.005223 0.005245 0.001988

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.22 -9.43 -1.31 -1.14 -1.00 0.82 -5.56 -4.59 -4.75 -2.28 -2.07 -11.90 -1.53 -1.46 -1.19
Bud Neck -6.49 -9.90 -13.07 -2.13 7.56 4.39 -10.90 -8.80 -10.68 1.86 -3.08 -14.58 -15.57 -14.06 6.66
Bud Site -1.97 -2.86 -6.83 -3.21 -5.52 2.01 -5.07 -6.56 -7.95 -1.93 0.52 -5.95 -9.30 -9.45 -4.54
Cell Periphery -7.11 -8.11 -7.72 4.05 4.41 6.18 0.61 -0.50 -6.11 -1.01 -1.46 -5.85 -6.86 -5.12 0.50
Cytoplasm 14.94 29.64 23.28 -3.58 -17.92 -11.37 11.67 8.52 20.22 -4.22 2.69 26.66 23.97 20.23 -10.61
Cytoplasmic Foci -9.34 -10.83 -6.39 4.31 8.67 6.48 -4.22 -3.13 -7.10 0.69 -2.46 -11.90 -7.41 -6.06 6.14
Eisosomes -5.82 -10.05 -8.26 -0.19 6.28 4.64 -6.42 -6.36 -8.80 -0.63 -2.87 -12.30 -10.75 -9.22 2.78
Endoplasmic Reticulum -3.30 -4.26 -1.76 2.59 0.64 -0.66 2.74 -0.09 0.61 -1.54 -1.49 -0.22 -1.54 1.01 -1.45
Endosome -3.06 -7.04 -1.70 -1.24 -1.20 1.74 -4.28 -2.01 -2.21 -1.35 -1.41 -7.85 -2.25 -2.15 -1.66
Golgi -6.74 -5.58 4.29 7.38 6.77 6.84 7.63 7.85 2.53 1.33 0.83 2.82 8.74 7.63 6.63
Lipid Particles -5.18 -10.01 -10.03 0.80 0.92 4.35 -5.41 -5.12 -9.16 0.48 -2.21 -10.66 -11.54 -9.60 0.21
Mitochondria -2.51 -5.85 -2.91 -1.34 -0.31 1.11 1.88 -1.00 -1.14 -1.20 -1.21 -3.05 -2.39 -2.16 -1.89
Mitotic Spindle -1.70 -3.23 -2.74 1.24 1.54 1.93 -0.17 -1.27 -2.41 -1.24 -0.41 -2.68 -2.30 -1.91 -0.53
None 3.69 11.81 3.65 0.22 -3.31 0.99 5.82 3.19 2.69 -1.93 1.53 10.83 4.89 2.97 -3.28
Nuclear Periphery -2.51 -8.80 -10.69 2.24 8.39 5.04 -0.88 2.16 -8.10 5.34 -1.51 -9.19 -2.59 0.81 8.74
Nuclear Periphery Foci -6.92 -9.53 -8.28 5.28 6.60 4.69 0.70 1.87 -4.51 1.03 -1.04 -4.98 -2.68 -1.16 3.65
Nucleolus -3.11 -9.04 -2.05 -0.91 0.32 0.04 -2.75 -2.07 -1.65 -0.86 -1.33 -9.43 -2.65 -1.86 -0.79
Nucleus -7.99 -15.58 -10.42 3.18 12.33 6.99 -5.02 -3.55 -10.42 1.89 -2.16 -15.84 -10.69 -8.18 9.13
Peroxisomes -0.90 -3.00 -1.98 -1.94 -1.83 -0.43 -2.99 -3.26 -3.18 -2.14 -0.61 -3.66 -2.46 -2.42 -2.13
Vacuole -9.01 -12.80 -19.91 -2.82 5.50 5.00 -13.63 -11.73 -15.78 4.48 -3.71 -19.87 -23.30 -20.19 5.67
Vacuole Periphery -4.60 -3.44 3.61 5.59 5.61 5.40 5.54 5.87 1.79 1.40 2.75 3.66 6.85 5.48 5.05
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Cobalamin-independent methionine synthase; involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Met6

Met6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Met6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available