Standard name
Human Ortholog
Description Strand exchange protein; forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.81 0.98 1.0 0.93 0.95 0.87 0.73 0.77 0.75 0.66 0.55 0.57 0.98 1.0 0.99 – 0.92 0.88 0.59 0.78 0.57 0.91 0.91 0.84
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.31 0.06 0 0.12 0.06 0.34 0.43 0.31 0.4 0.5 0.68 0.6 0 0 0 – 0 0 0.21 0.1 0.22 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 – 0 0 2 1 7 0 1 3
Bud 2 0 0 3 4 4 17 9 9 9 10 2 0 0 2 – 0 0 0 0 5 0 1 13
Bud Neck 0 0 0 2 3 2 3 1 3 1 0 1 1 5 0 – 0 0 0 1 4 0 0 2
Bud Site 0 0 0 0 1 1 1 7 0 2 1 2 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 2 1 3 0 0 0 1 0 0 1 2 8 1 – 6 1 0 0 4 0 0 2
Cytoplasm 101 124 252 213 300 284 340 347 258 252 139 174 253 421 450 – 130 89 82 105 144 108 224 313
Endoplasmic Reticulum 1 0 0 0 0 0 0 0 0 0 0 3 2 1 0 – 7 7 4 0 2 0 2 1
Endosome 0 0 1 0 4 0 1 0 1 0 1 0 1 0 2 – 2 4 2 3 5 0 1 1
Golgi 0 0 1 1 0 0 0 0 0 0 0 0 1 0 0 – 1 0 0 0 3 0 0 0
Mitochondria 38 8 2 27 20 112 200 139 137 191 172 183 0 1 1 – 3 3 29 13 55 2 6 9
Nucleus 0 0 0 1 1 1 2 3 1 1 2 1 0 0 2 – 0 0 0 0 0 1 0 0
Nuclear Periphery 0 0 0 1 0 0 0 1 0 0 0 2 0 0 1 – 0 0 1 0 1 0 0 2
Nucleolus 0 0 0 1 1 0 1 1 0 1 0 1 0 0 0 – 1 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 1 0 0 1 0 0 0 1 0 – 1 0 1 0 0 0 0 0
SpindlePole 1 0 3 2 3 2 7 27 11 10 6 9 0 0 0 – 1 3 3 2 5 0 1 8
Vac/Vac Membrane 0 0 1 1 0 1 2 8 0 2 4 4 0 0 0 – 8 5 4 4 6 1 3 2
Unique Cell Count 124 127 253 228 315 328 463 448 345 382 252 304 257 423 455 141 101 140 136 253 120 248 374
Labelled Cell Count 143 132 262 253 340 407 575 543 421 470 335 383 260 437 460 160 112 140 136 253 120 248 374


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.1 3.9 4.1 3.4 3.7 3.0 3.0 3.0 2.8 2.7 2.5 2.5 4.7 4.7 4.8 4.8 5.1 5.8 4.2 4.8 5.4
Std Deviation (1e-4) 0.6 0.9 4.5 1.5 1.7 1.2 1.6 1.8 1.4 1.2 1.3 1.2 1.0 1.2 1.2 1.3 1.5 2.6 1.3 1.2 1.4
Intensity Change (Log2) – – – -0.25 -0.15 -0.45 -0.46 -0.43 -0.55 -0.59 -0.69 -0.7 0.22 0.2 0.22 0.23 0.33 0.51 0.04 0.24 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0044 -0.1159 -0.1075 -0.1758 0.2166 -0.2047 -0.9682 -1.1017 -1.1536 -1.2062 -1.4337 -1.1115 -2.5534 -2.0506 -2.5875 -1.9923 -1.9825 -2.2357
Actin 0.0467 0.0005 0.0139 0.0001 0.0455 0.0073 0.0338 0.0034 0.0165 0.0001 0.0007 0.0182 0.0082 0.0015 0.002 0.0001 0.0196 0.0034
Bud 0.0014 0.0009 0.0019 0 0.0003 0.0001 0.002 0.0109 0.0007 0.0001 0.0001 0.001 0.0021 0.0047 0.0002 0.0001 0.001 0.0001
Bud Neck 0.0032 0.0004 0.0015 0 0.0022 0.0001 0.0033 0.0002 0.0007 0.0001 0.0001 0.0007 0.0009 0.0001 0 0 0.0002 0.0001
Bud Periphery 0.0029 0.0008 0.0047 0.0001 0.0006 0.0004 0.0078 0.0146 0.001 0 0.0001 0.004 0.0017 0.0039 0.0001 0.0001 0.0015 0.0001
Bud Site 0.0056 0.0125 0.0069 0 0.0013 0 0.0098 0.0157 0.008 0.0002 0.0005 0.0005 0.0111 0.0019 0.0002 0 0.0004 0.0002
Cell Periphery 0.0004 0.0002 0.0005 0 0.0003 0 0.0004 0.0004 0.0001 0 0 0.0001 0.0003 0.0002 0 0 0.0001 0
Cytoplasm 0.0181 0.0298 0.017 0.0201 0.0479 0.025 0.0525 0.114 0.1031 0.1675 0.1057 0.1584 0.0274 0.0365 0.0387 0.0209 0.0272 0.0618
Cytoplasmic Foci 0.0199 0.0027 0.0124 0.0019 0.0077 0.005 0.0456 0.0119 0.008 0.0056 0.0251 0.0128 0.0217 0.0124 0.0069 0.0088 0.0661 0.0091
Eisosomes 0.0006 0.0002 0.0047 0.0002 0.0006 0.0001 0.0004 0.0002 0.0002 0.0001 0 0.0001 0.0003 0.0001 0.0001 0.0001 0.0003 0.0001
Endoplasmic Reticulum 0.0033 0.0004 0.0008 0.0001 0.0032 0.0003 0.0048 0.0013 0.0008 0.001 0.0013 0.0016 0.0046 0.0009 0.0004 0.0003 0.0014 0.0005
Endosome 0.0095 0.0028 0.0041 0.0001 0.04 0.0008 0.0531 0.002 0.0017 0.0352 0.0062 0.0123 0.0107 0.0014 0.0005 0.0007 0.0043 0.0085
Golgi 0.0053 0.0001 0.0021 0 0.0261 0.0009 0.0143 0.0007 0.0023 0.0009 0.0043 0.0094 0.0026 0.0007 0.0007 0.0001 0.0062 0.0033
Lipid Particles 0.0093 0.0002 0.0064 0.0001 0.022 0.0016 0.0518 0.0007 0.0052 0.0001 0.0101 0.0075 0.0063 0.0043 0.0035 0.0003 0.0211 0.007
Mitochondria 0.0075 0.0004 0.0027 0.0001 0.0097 0.0003 0.0119 0.0065 0.0103 0.0003 0.0009 0.0173 0.0031 0.0015 0.0034 0.0005 0.0075 0.0021
None 0.8357 0.9363 0.8967 0.9767 0.7575 0.9551 0.6246 0.8118 0.833 0.7807 0.8294 0.7395 0.8594 0.9221 0.9387 0.9583 0.7744 0.9003
Nuclear Periphery 0.0043 0.0007 0.0019 0.0001 0.0084 0.0005 0.01 0.0008 0.0005 0.0008 0.0005 0.0036 0.0193 0.0003 0.0004 0.0003 0.0009 0.0006
Nucleolus 0.0014 0.0008 0.0018 0 0.0009 0.0001 0.0048 0.0005 0.0001 0 0.0001 0.0005 0.0018 0.0002 0.0003 0.0003 0.0001 0
Nucleus 0.0027 0.0022 0.0026 0.0001 0.0017 0.0003 0.0034 0.0008 0.0003 0.0006 0.0005 0.0019 0.0088 0.0013 0.0005 0.0004 0.0003 0.0002
Peroxisomes 0.0154 0.0002 0.0083 0.0001 0.0137 0.0009 0.0138 0.0005 0.006 0.0001 0.0113 0.0031 0.0031 0.0044 0.0008 0.0001 0.065 0.0009
Punctate Nuclear 0.0051 0.0018 0.0074 0.0001 0.004 0.001 0.0394 0.0017 0.0009 0.0002 0.0019 0.004 0.0042 0.0006 0.0021 0.0077 0.0011 0.0004
Vacuole 0.0011 0.0048 0.0013 0.0001 0.0032 0.0001 0.0086 0.0009 0.0003 0.0049 0.001 0.0027 0.0015 0.0008 0.0002 0.0009 0.0009 0.001
Vacuole Periphery 0.0006 0.0012 0.0004 0 0.0031 0 0.004 0.0003 0.0002 0.0014 0.0002 0.0009 0.001 0.0001 0.0001 0.0002 0.0005 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 77.0457 40.9965 21.2948 31.5605 88.0011 78.8364 26.3217 21.2625 34.3188 58.5162
Translational Efficiency 1.9056 1.3416 1.31 2.3257 1.2932 2.201 1.9275 1.8439 1.4428 1.698

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
237 170 1289 898 133 1675 240 965 370 1845 1529 1863

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 663.02 775.61 788.79 752.42 701.40 689.77 884.27 758.77 676.82 697.68 803.78 755.71
Standard Deviation 93.03 101.72 87.44 113.18 78.33 86.84 90.87 102.78 89.93 91.74 94.60 107.97
Intensity Change Log 2 — 0.226279 0.250589 0.182486 — -0.024122 0.334250 0.113425 — 0.102987 0.294201 0.147397

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000137 0.001306 0.000179 0.000554 0.000061 0.000321 0.000373 0.001076 0.000110 0.000412 0.000209 0.000824
Bud Neck 0.013607 0.043589 0.003286 0.007222 0.002096 0.044430 0.001956 0.004721 0.009469 0.044353 0.003077 0.005926
Bud Site 0.002191 0.018425 0.004731 0.012627 0.001280 0.011956 0.003659 0.031285 0.001863 0.012552 0.004563 0.022292
Cell Periphery 0.000185 0.000234 0.000135 0.000228 0.000309 0.000210 0.000191 0.000239 0.000230 0.000213 0.000144 0.000234
Cytoplasm 0.351098* 0.251775* 0.251196* 0.437734* 0.534866* 0.355196* 0.179255* 0.463716* 0.417155* 0.345667* 0.239904* 0.451192*
Cytoplasmic Foci 0.322432* 0.332858* 0.009278 0.034474 0.215919* 0.246630* 0.017288 0.028130 0.284145* 0.254575* 0.010535 0.031188
Eisosomes 0.000237 0.000325 0.000039 0.000071 0.000158 0.000197 0.000106 0.000061 0.000209 0.000209 0.000049 0.000066
Endoplasmic Reticulum 0.000764 0.001585 0.002729 0.003450 0.000602 0.000666 0.004796 0.002271 0.000706 0.000751 0.003054 0.002839
Endosome 0.014774 0.049789 0.001693 0.004774 0.007888 0.034157 0.003788 0.004155 0.012299 0.035597 0.002022 0.004454
Golgi 0.001501 0.008714 0.000058 0.001845 0.000833 0.006863 0.000099 0.002316 0.001261 0.007034 0.000064 0.002089
Lipid Particles 0.015450 0.008856 0.001486 0.002409 0.006220 0.011716 0.003779 0.001620 0.012132 0.011452 0.001846 0.002000
Mitochondria 0.006006 0.015317 0.000693 0.002094 0.001708 0.023539 0.000994 0.001239 0.004461 0.022782 0.000740 0.001651
Mitotic Spindle 0.000128 0.005849 0.002515 0.009824 0.001664 0.012028 0.003476 0.017307 0.000680 0.011459 0.002665 0.013700
None 0.001478 0.000638 0.004024 0.006183 0.003934 0.002820 0.005295 0.006194 0.002361 0.002619 0.004224 0.006189
Nuclear Periphery 0.000642 0.000644 0.001983 0.001983 0.000311 0.000613 0.002049 0.001087 0.000523 0.000616 0.001993 0.001519
Nuclear Periphery Foci 0.000523 0.000916 0.000599 0.001673 0.000645 0.000272 0.001364 0.001025 0.000567 0.000331 0.000719 0.001337
Nucleolus 0.001185 0.001797 0.000641 0.000775 0.001090 0.001821 0.001201 0.000384 0.001151 0.001819 0.000729 0.000572
Nucleus 0.204751* 0.101061 0.686421* 0.355118* 0.186387* 0.133981* 0.677975* 0.329542* 0.198150* 0.130947* 0.685095* 0.341870*
Peroxisomes 0.017437 0.041745 0.000256 0.000868 0.001618 0.020319 0.001873 0.001815 0.011751 0.022293 0.000509 0.001359
Vacuole 0.044843 0.111142 0.027896 0.114002 0.031936 0.089504 0.090283 0.101049 0.040204 0.091498 0.037689 0.107293
Vacuole Periphery 0.000632 0.003435 0.000162 0.002092 0.000475 0.002760 0.000199 0.000768 0.000575 0.002822 0.000168 0.001406

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.95 -2.94 -3.18 1.31 -2.22 -5.67 -5.28 -2.37 -1.34 -1.08 -4.19 -5.46 -3.33 -1.13 -2.19
Bud Neck -3.78 4.08 2.85 4.87 -2.34 -16.80 0.01 -4.96 15.18 -4.72 -12.45 3.69 2.19 15.77 -3.13
Bud Site -3.99 -2.16 -4.50 1.88 -2.64 -9.60 -1.43 -5.66 -2.04 -4.78 -9.84 -2.62 -7.00 -0.93 -4.97
Cell Periphery -1.32 2.19 0.63 1.67 -0.97 3.00 3.59 3.74 1.17 0.50 0.92 4.59 2.66 1.76 -1.39
Cytoplasm 4.20 7.16 0.92 -4.13 -9.24 8.56 16.13 7.68 -0.99 -13.67 4.85 13.77 4.51 -0.52 -14.94
Cytoplasmic Foci -0.36 21.40 20.24 18.23 -7.48 -2.10 14.08 13.63 41.17 -2.90 2.42 24.94 23.63 44.85 -9.58
Eisosomes -0.87 8.61 7.12 2.50 -7.07 -2.39 2.04 6.05 13.67 5.61 0.12 9.84 8.66 10.81 -5.26
Endoplasmic Reticulum -2.08 -11.81 -11.75 -6.38 -2.93 -0.40 -10.93 -11.94 -13.02 5.43 -0.32 -16.39 -15.64 -17.82 0.54
Endosome -5.46 6.66 5.83 7.63 -3.96 -10.17 2.41 3.14 14.76 1.91 -9.64 7.36 6.60 16.32 -3.37
Golgi -2.46 4.19 0.96 2.65 -1.29 -7.22 2.02 -0.30 5.92 -1.55 -7.46 4.65 0.62 7.14 -2.03
Lipid Particles 2.43 5.71 5.47 5.09 -3.15 -5.17 2.98 6.51 12.23 6.19 0.45 6.38 6.42 12.67 0.32
Mitochondria -2.01 2.22 1.96 3.59 -1.58 -9.11 0.86 0.76 10.82 -0.38 -7.33 2.37 2.14 11.18 -1.51
Mitotic Spindle -2.27 -3.01 -5.13 -1.99 -3.80 -3.95 -0.40 -3.07 0.40 -2.66 -5.54 -2.13 -6.03 -0.16 -4.66
None 3.13 -5.66 -3.93 -5.26 -1.16 1.19 -0.04 -0.26 -1.55 -0.17 -0.37 -3.07 -3.45 -2.32 -1.21
Nuclear Periphery -0.03 -15.43 -12.51 -10.44 -2.90 -3.91 -17.26 -15.36 -10.38 4.23 -1.07 -22.06 -16.95 -15.43 0.50
Nuclear Periphery Foci -1.72 -2.16 -5.17 -3.15 -4.34 3.46 -5.35 -4.15 -7.39 1.49 2.59 -3.72 -6.55 -9.35 -4.29
Nucleolus -1.47 3.09 3.09 3.32 0.33 -3.93 0.45 4.51 9.44 2.58 -3.76 3.50 4.96 9.59 2.74
Nucleus 5.87 -32.64 -11.23 -18.77 25.97 3.33 -19.11 -9.76 -23.06 13.80 6.33 -39.85 -14.66 -31.96 32.69
Peroxisomes -2.80 4.60 4.49 5.43 -1.56 -13.17 0.60 1.46 13.69 0.27 -3.75 4.67 4.50 14.83 -1.75
Vacuole -5.72 0.75 -17.10 -6.59 -19.30 -10.67 -8.89 -18.42 -12.40 -2.74 -10.99 -4.03 -24.35 -16.73 -21.96
Vacuole Periphery -3.89 5.50 -0.77 2.89 -1.84 -12.29 4.10 0.39 10.53 -1.64 -12.51 6.67 -0.48 7.53 -2.30
Standard name
Human Ortholog
Description Strand exchange protein; forms a helical filament with DNA that searches for homology; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; homolog of Dmc1p and bacterial RecA protein
Localization
Cell Percentages cytoplasm (22%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rad51

Rad51


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rad51-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available