Standard name
Human Ortholog
Description DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; Slt2p-independent regulator of cold growth; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0
Cytoplasm 0.2 0.13 0.18 0.14 0.18 0.13 0.1 0.11 0.08 – 0.07 0.05 0.13 0.22 0.24 0.05 – – 0.13 0.06 0.07 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0
Endosome 0.05 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 – – 0.05 0.05 0.06 0 0.08 0.09
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0
Mitochondria 0 0.14 0.37 0.45 0.24 0.45 0.48 0.55 0.65 – 0.71 0.72 0.07 0.08 0.2 0.05 – – 0 0 0.05 0 0 0.06
Nucleus 0.67 0.69 0.58 0.6 0.59 0.59 0.57 0.52 0.49 – 0.52 0.47 0.8 0.59 0.53 0.67 – – 0.45 0.54 0.4 0.54 0.4 0.26
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.05 0.1 0.1 0.13 0.17 – 0.16 0.16 0.06 0 0.05 0.05 – – 0.13 0.11 0.11 0.15 0.12 0.13
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0.08 0.07 0.11 0.07 0.11 0.16
Vac/Vac Membrane 0.05 0.08 0 0 0 0 0 0 0 – 0 0 0 0.12 0.06 0.14 – – 0.06 0.09 0.13 0.12 0.16 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 1
Bud 0 0 0 0 3 1 4 12 8 – 9 9 0 0 0 0 – – 1 8 4 1 4 2
Bud Neck 0 0 0 1 0 1 2 3 1 – 1 1 0 0 0 0 – – 0 1 1 2 1 3
Bud Site 0 0 0 0 2 9 9 13 14 – 15 13 0 0 0 0 – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0
Cytoplasm 33 37 45 29 50 44 48 62 30 – 24 19 24 49 59 1 – – 26 20 22 6 7 7
Endoplasmic Reticulum 0 4 1 0 0 0 0 1 0 – 0 0 0 0 2 0 – – 0 0 1 0 0 1
Endosome 9 7 4 1 10 2 4 4 1 – 1 0 3 6 2 1 – – 11 15 19 5 17 19
Golgi 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 5 3 1 1 0
Mitochondria 3 40 92 93 67 150 226 325 243 – 254 289 13 17 49 1 – – 7 6 13 2 7 14
Nucleus 112 195 145 124 163 196 268 305 181 – 187 188 152 133 130 14 – – 93 178 122 95 92 59
Nuclear Periphery 0 0 1 1 2 0 7 8 2 – 6 8 1 0 1 0 – – 2 3 2 0 2 2
Nucleolus 6 8 4 5 13 32 46 78 64 – 56 66 11 5 12 1 – – 27 34 34 26 26 30
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0
SpindlePole 6 2 13 1 9 5 21 16 8 – 5 4 3 4 3 0 – – 16 22 32 11 24 36
Vac/Vac Membrane 8 22 5 4 9 8 15 9 9 – 1 9 3 27 15 3 – – 12 28 38 20 36 37
Unique Cell Count 168 282 252 206 276 333 474 586 372 359 403 191 225 243 21 207 328 305 175 229 227
Labelled Cell Count 177 316 310 259 328 448 650 836 561 559 606 210 241 273 21 207 328 305 175 229 227


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 4.7 4.1 3.8 4.6 4.2 4.1 3.9 3.5 – 3.6 3.6 5.1 4.7 4.1 7.4 – – 4.7 4.7 5.4
Std Deviation (1e-4) 0.9 0.9 1.2 1.0 1.6 1.4 1.4 1.3 1.3 – 1.3 1.4 1.1 1.0 1.1 1.5 – – 1.0 1.3 1.6
Intensity Change (Log2) – – – -0.1 0.15 0.03 -0.02 -0.08 -0.22 – -0.18 -0.18 0.3 0.18 0.0 0.86 – – 0.2 0.21 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5552 2.0938 2.0559 1.5495 2.0714 1.7767 3.3529 3.4752 3.4265 2.855 2.2869 2.9789 3.1336 4.5547 3.7388 3.7 3.1911 3.6144
Actin 0.0685 0.0004 0.0182 0.0045 0.0544 0.0023 0.0276 0.0014 0.0044 0.0045 0.0098 0.0056 0.0188 0.0009 0.0107 0.0306 0.0028 0.0006
Bud 0.0017 0.0022 0.0009 0.0133 0.0086 0.0004 0.0012 0.0044 0.0016 0.0157 0.031 0.0008 0.0007 0.0281 0.0011 0.0106 0.0476 0.0001
Bud Neck 0.0083 0.0012 0.0017 0.0236 0.008 0.0054 0.0056 0.0016 0.0011 0.0031 0.0081 0.0049 0.004 0.0015 0.0009 0.0032 0.0046 0.0015
Bud Periphery 0.0039 0.0022 0.0014 0.02 0.0128 0.0012 0.002 0.005 0.0024 0.0206 0.0408 0.0015 0.0022 0.0387 0.0015 0.0153 0.0622 0.0004
Bud Site 0.0053 0.0053 0.0095 0.01 0.0046 0.0005 0.0094 0.0082 0.0035 0.0052 0.0053 0.0006 0.0037 0.0394 0.001 0.0099 0.0043 0.0004
Cell Periphery 0.0006 0.0003 0.0003 0.0018 0.0009 0.0002 0.0006 0.0008 0.0008 0.0006 0.0009 0.0001 0.0004 0.0017 0.0001 0.0022 0.0012 0.0001
Cytoplasm 0.008 0.0129 0.0367 0.1983 0.3434 0.0233 0.0222 0.0311 0.0285 0.1734 0.271 0.0205 0.0068 0.081 0.0434 0.4209 0.418 0.0222
Cytoplasmic Foci 0.0092 0.0011 0.0042 0.016 0.0162 0.0017 0.0183 0.0008 0.0069 0.0153 0.0207 0.0027 0.0064 0.0018 0.0045 0.0146 0.0096 0.0047
Eisosomes 0.0006 0 0.0002 0.0003 0.0008 0 0.0003 0 0.0002 0.0002 0.0001 0 0.0002 0.0001 0 0.0012 0.0001 0
Endoplasmic Reticulum 0.0057 0.0026 0.0023 0.0165 0.0114 0.0007 0.0047 0.0011 0.0039 0.0085 0.0129 0.001 0.0011 0.0043 0.0017 0.0102 0.0083 0.0008
Endosome 0.023 0.0083 0.0087 0.0342 0.0267 0.0021 0.034 0.0007 0.0181 0.0271 0.0518 0.0061 0.0163 0.0015 0.0025 0.0102 0.0061 0.0088
Golgi 0.0085 0.0004 0.0043 0.0029 0.009 0.0005 0.0089 0.0001 0.0058 0.0022 0.0062 0.0025 0.0055 0.0002 0.0078 0.0064 0.0004 0.0007
Lipid Particles 0.0145 0.0005 0.0104 0.0336 0.0207 0.001 0.027 0.0002 0.0024 0.0044 0.0099 0.0061 0.0085 0.0003 0.0003 0.0276 0.0004 0.002
Mitochondria 0.0085 0.001 0.0114 0.0043 0.0233 0.0064 0.0116 0.0006 0.0112 0.013 0.0082 0.0061 0.0054 0.0012 0.0017 0.0162 0.0044 0.0089
None 0.0083 0.0107 0.0206 0.1983 0.0873 0.0046 0.0133 0.0125 0.0073 0.1293 0.1212 0.0202 0.0019 0.1582 0.0249 0.1733 0.1408 0.0031
Nuclear Periphery 0.0757 0.0187 0.0175 0.017 0.0307 0.0072 0.0373 0.0145 0.0108 0.0317 0.0357 0.0074 0.0137 0.0077 0.0109 0.0145 0.0088 0.0106
Nucleolus 0.0342 0.0422 0.019 0.0105 0.0171 0.0824 0.0367 0.0352 0.0167 0.0195 0.0129 0.0686 0.0464 0.011 0.0251 0.0063 0.0072 0.075
Nucleus 0.6538 0.8489 0.7814 0.3459 0.2182 0.8315 0.6931 0.8485 0.8557 0.4827 0.3029 0.8199 0.7928 0.6036 0.8347 0.1683 0.2399 0.8433
Peroxisomes 0.009 0.0001 0.0086 0.0019 0.0242 0.0006 0.0107 0.0001 0.0016 0.0016 0.0058 0.004 0.0104 0.0001 0.0126 0.0273 0.0004 0.0005
Punctate Nuclear 0.0427 0.033 0.0389 0.0213 0.057 0.0264 0.0292 0.0305 0.012 0.0254 0.0238 0.0193 0.05 0.0083 0.0126 0.0084 0.0066 0.0144
Vacuole 0.0059 0.0059 0.0023 0.0219 0.0193 0.0013 0.0043 0.0021 0.0039 0.0119 0.0157 0.0011 0.0037 0.0095 0.0015 0.0184 0.022 0.0011
Vacuole Periphery 0.0041 0.0019 0.0015 0.0037 0.0053 0.0004 0.0023 0.0004 0.0013 0.004 0.0054 0.0007 0.0012 0.001 0.0005 0.0044 0.0044 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.7278 4.2076 8.4746 32.9984 21.7948 17.1366 10.1039 8.7166 18.7353 35.1224
Translational Efficiency 0.9965 0.9506 1.0196 0.4602 0.876 2.0135 0.6129 0.7268 0.7634 0.7474

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
69 179 335 987 1920 1372 1037 352 1989 1551 1372 1339

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 651.22 772.14 1041.99 859.58 685.61 736.37 940.42 902.60 684.42 740.50 965.22 870.89
Standard Deviation 65.56 101.65 131.72 121.50 123.01 99.38 114.19 114.48 121.64 100.30 126.48 121.19
Intensity Change Log 2 — 0.245717 0.678125 0.400487 — 0.103043 0.455917 0.396699 — 0.174308 0.568439 0.398545

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000026 0.001947 0.001351 0.001029 0.000183 0.000320 0.001335 0.001047 0.000178 0.000508 0.001339 0.001034
Bud Neck 0.004322 0.007987 0.007905 0.014984 0.005455 0.011266 0.020271 0.009554 0.005416 0.010888 0.017251 0.013557
Bud Site 0.000697 0.004474 0.002894 0.019481 0.001556 0.003057 0.007980 0.008210 0.001526 0.003221 0.006738 0.016518
Cell Periphery 0.000218 0.000177 0.000123 0.000230 0.000149 0.000156 0.000162 0.000147 0.000151 0.000159 0.000152 0.000208
Cytoplasm 0.234767* 0.093393 0.078190 0.257383* 0.117802* 0.108600* 0.054229 0.174474* 0.121860* 0.106845* 0.060079 0.235588*
Cytoplasmic Foci 0.061834 0.031624 0.003621 0.018223 0.022284 0.028836 0.004128 0.018909 0.023656 0.029158 0.004004 0.018403
Eisosomes 0.000114 0.000105 0.000039 0.000048 0.000066 0.000052 0.000048 0.000055 0.000068 0.000058 0.000046 0.000050
Endoplasmic Reticulum 0.000294 0.006463 0.006995 0.005216 0.001846 0.002561 0.008755 0.006639 0.001792 0.003012 0.008326 0.005590
Endosome 0.003129 0.005854 0.001414 0.014439 0.001445 0.004171 0.001940 0.009276 0.001503 0.004365 0.001812 0.013082
Golgi 0.000205 0.000925 0.000218 0.007128 0.000771 0.001531 0.000199 0.002373 0.000751 0.001461 0.000204 0.005878
Lipid Particles 0.005276 0.006925 0.000741 0.001816 0.003814 0.006352 0.000746 0.001739 0.003865 0.006418 0.000745 0.001796
Mitochondria 0.000601 0.004437 0.001103 0.010254 0.003348 0.011932 0.002107 0.001366 0.003252 0.011067 0.001862 0.007917
Mitotic Spindle 0.000103 0.008592 0.002619 0.101098* 0.001824 0.010667 0.008621 0.062738 0.001765 0.010427 0.007156 0.091014
None 0.026651 0.015827 0.007261 0.019099 0.046151 0.015052 0.005321 0.015223 0.045475 0.015141 0.005794 0.018080
Nuclear Periphery 0.000715 0.001813 0.001119 0.002793 0.003191 0.003992 0.001979 0.002245 0.003105 0.003741 0.001769 0.002649
Nuclear Periphery Foci 0.000336 0.008952 0.001880 0.002415 0.003474 0.002124 0.002148 0.003608 0.003365 0.002912 0.002083 0.002728
Nucleolus 0.014104 0.055533 0.018711 0.010372 0.044581 0.041861 0.027138 0.008642 0.043523 0.043439 0.025080 0.009917
Nucleus 0.637006* 0.728306* 0.849673* 0.456508* 0.737670* 0.735063* 0.834508* 0.614920* 0.734178* 0.734283* 0.838211* 0.498152*
Peroxisomes 0.001104 0.006896 0.000503 0.001564 0.001637 0.004691 0.000793 0.000609 0.001618 0.004945 0.000722 0.001313
Vacuole 0.008241 0.009049 0.013428 0.052411 0.002493 0.006413 0.017284 0.054854 0.002692 0.006717 0.016342 0.053054
Vacuole Periphery 0.000257 0.000721 0.000209 0.003509 0.000261 0.001302 0.000309 0.003373 0.000261 0.001235 0.000284 0.003473

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.04 -12.60 -11.69 1.45 1.88 -4.52 -18.21 -11.52 -10.54 -2.07 -6.06 -21.46 -13.98 -9.38 -0.12
Bud Neck -2.06 -4.11 -8.17 -5.39 -3.57 -5.39 -13.00 -9.65 -5.50 2.08 -5.58 -13.37 -13.07 -6.94 1.53
Bud Site -3.58 -5.36 -8.45 -4.79 -4.51 -1.94 -4.76 -4.49 -3.94 -2.41 -2.42 -5.56 -8.84 -7.43 -4.51
Cell Periphery 0.35 1.03 0.62 0.68 -1.52 -0.46 -0.75 -2.74 -1.84 -1.95 -0.65 -0.59 -2.73 -1.56 -2.19
Cytoplasm 4.23 5.53 2.09 -5.01 -10.08 1.01 12.64 0.52 -0.12 -6.35 2.02 13.39 -3.77 -5.29 -14.40
Cytoplasmic Foci 2.06 4.47 4.07 3.95 -4.40 -1.83 11.16 7.45 8.35 -2.35 -1.55 12.13 9.02 9.71 -4.93
Eisosomes -0.39 1.84 1.32 3.41 -2.22 0.96 0.82 -1.68 -6.13 -5.98 0.54 0.84 -0.82 -4.82 -6.61
Endoplasmic Reticulum -6.46 -12.97 -19.43 -3.09 -0.23 -3.28 -15.36 -10.86 -10.19 -3.04 -5.64 -18.61 -19.57 -16.26 -2.45
Endosome -0.87 0.93 -1.30 -0.03 -3.61 -4.11 -1.53 -2.02 0.08 -1.73 -4.40 -1.41 -4.50 -0.92 -4.13
Golgi -3.45 -1.07 -2.36 -1.23 -2.22 -1.80 2.39 0.10 1.60 -1.44 -1.89 2.30 -1.55 -0.45 -2.47
Lipid Particles -1.37 1.92 1.76 4.64 -1.19 -2.63 8.67 6.13 6.70 -4.10 -3.08 8.60 6.66 7.78 -4.61
Mitochondria -6.60 -3.66 -3.97 -0.02 -3.03 -5.43 2.12 2.44 6.37 0.78 -5.56 2.48 -0.93 4.51 -2.44
Mitotic Spindle -3.45 -3.20 -12.71 -9.74 -10.56 -4.83 -5.77 -6.88 -5.82 -5.35 -5.34 -6.22 -14.14 -11.89 -11.36
None 1.66 2.96 2.28 1.82 -3.52 8.43 12.39 9.91 2.57 -4.20 8.62 12.60 10.15 2.79 -5.05
Nuclear Periphery -3.30 -4.70 -10.11 -6.38 -7.70 -1.33 1.05 -3.17 -2.11 -3.82 -1.19 1.50 -5.00 -3.20 -7.45
Nuclear Periphery Foci -3.57 -4.17 -5.35 2.67 1.02 3.65 1.62 -0.09 -2.05 -1.14 1.39 1.19 0.91 -0.48 -0.33
Nucleolus -4.27 -0.55 1.13 6.42 2.92 1.71 3.22 9.22 7.52 5.62 0.85 4.40 13.38 12.27 8.22
Nucleus -1.80 -5.02 1.56 5.87 16.16 0.02 -8.77 6.82 6.61 11.35 -0.09 -10.32 13.14 12.56 21.86
Peroxisomes -3.67 1.35 1.21 3.96 -0.33 -3.97 4.72 5.41 5.96 0.96 -4.84 4.92 4.87 6.75 -0.07
Vacuole 0.30 -3.37 -12.15 -13.67 -8.22 -4.64 -7.50 -9.92 -9.53 -8.10 -4.90 -9.25 -18.43 -17.43 -13.26
Vacuole Periphery -2.27 -0.07 -2.41 -1.30 -2.62 -3.74 -1.87 -1.22 0.47 -1.13 -3.95 -1.58 -2.88 0.16 -2.75
Standard name
Human Ortholog
Description DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; Slt2p-independent regulator of cold growth; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p
Localization
Cell Percentages nucleus (94%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Swi4

Swi4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Swi4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available