Standard name
Human Ortholog
Description Transmembrane osmosensor for filamentous growth and HOG pathways; involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol (HOG) response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.23 0.22 0.23 0.19 0.11 0.1 0 0 – 0 0 0 0.23 0.25 0.13 0.05 0.15 0.13 0.15 0.14 0.26 0.17 0.18 0.15
Bud 0 0.06 0.15 0.09 0.11 0.11 0.1 0.09 – 0.08 0.1 0.09 0.11 0.09 0 0 0 0 0.06 0.11 0 0.18 0.21 0.16
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.09 0.12 0.06 0.15 0.12 0.1
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0.05 0.08 0.05 0 0 0 0 0 0 0 0 0
Cytoplasm 0.35 0.52 0.42 0.59 0.65 0.68 0.7 0.74 – 0.74 0.78 0.75 0.46 0.47 0.59 0.44 0.42 0.38 0 0.06 0 0 0 0
Endoplasmic Reticulum 0.24 0.27 0.22 0.13 0.06 0.08 0 0 – 0 0 0 0.27 0.27 0.26 0 0 0 0.27 0.24 0.23 0.07 0.08 0.12
Endosome 0.06 0 0.05 0 0.1 0 0.05 0 – 0 0 0 0 0.05 0.06 0.05 0.06 0 0.06 0 0.1 0.06 0.06 0.06
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0 0 0.08 0 0 0.09 0.15 0.14 – 0.13 0.23 0.22 0 0 0 0.07 0.07 0 0 0 0.06 0.06 0.06 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.07 0.07 0 0.07 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0 0 0 0.07 0 0.05 0.05 – 0.08 0 0.05 0 0 0.07 0.44 0.41 0.4 0.28 0.24 0.17 0.2 0.23 0.28
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 54 30 74 64 52 68 30 11 – 19 17 14 30 38 22 4 12 7 47 25 104 21 47 35
Bud 10 8 47 32 53 75 87 74 – 62 61 65 14 14 2 0 3 0 17 20 14 22 53 35
Bud Neck 0 0 0 1 2 1 3 0 – 3 1 1 0 0 0 0 0 0 28 21 22 18 31 22
Bud Site 7 6 9 11 7 14 10 5 – 1 4 3 1 1 0 0 0 0 – – – – – –
Cell Periphery 4 1 3 3 2 1 7 8 – 11 7 5 6 12 8 0 0 1 1 0 4 0 0 0
Cytoplasm 83 72 133 202 320 452 586 617 – 595 452 551 60 72 101 32 34 21 6 10 7 3 5 5
Endoplasmic Reticulum 57 37 70 45 29 51 28 19 – 20 5 12 36 41 44 3 2 2 84 43 93 8 20 28
Endosome 14 2 17 6 49 28 39 24 – 35 5 8 1 7 11 4 5 1 18 6 40 7 14 14
Golgi 0 0 0 1 0 0 0 0 – 0 0 0 0 1 1 2 0 3 10 1 13 1 1 4
Mitochondria 1 2 26 11 13 62 124 121 – 107 133 163 1 0 0 5 6 2 4 3 24 8 14 8
Nucleus 1 0 0 2 3 3 4 10 – 5 2 3 0 0 0 0 0 0 0 0 0 0 2 1
Nuclear Periphery 0 0 1 2 1 5 2 4 – 4 0 0 0 0 0 0 0 0 0 0 1 0 1 1
Nucleolus 0 1 1 0 1 0 3 10 – 3 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 18 10 11 24 23 9 14 4 – 3 3 2 1 2 0 0 1 0 1 0 3 3 2 1
SpindlePole 3 2 7 8 13 13 19 13 – 10 2 9 1 2 1 0 1 0 2 2 0 0 0 1
Vac/Vac Membrane 44 5 11 8 32 30 40 42 – 61 23 37 2 4 12 32 33 22 88 42 70 25 60 64
Unique Cell Count 240 139 318 342 492 667 835 836 800 583 733 131 154 172 73 81 55 315 181 407 125 258 232
Labelled Cell Count 296 176 410 420 600 812 996 962 939 715 874 153 194 202 82 97 59 315 181 407 125 258 232


Bud Neck, Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.2 8.6 6.0 6.0 5.8 5.5 4.6 4.9 – 4.5 4.3 4.3 7.6 8.1 8.5 10.9 10.2 10.7 8.7 9.0 8.8
Std Deviation (1e-4) 1.1 1.5 1.0 1.4 1.2 1.5 1.1 1.2 – 1.3 0.9 0.9 1.1 1.4 1.7 2.4 2.4 2.4 1.4 1.7 2.1
Intensity Change (Log2) – – – 0.01 -0.03 -0.11 -0.38 -0.3 – -0.39 -0.47 -0.48 0.34 0.44 0.51 0.86 0.78 0.84 0.55 0.59 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.4 -4.9 -5.5 0 0 – 0 0 0 -0.1 0.3 -2.8 -3.4 -1.7 -1.8
Bud -2.1 -1.7 -1.6 0 0 – 0 0 0 -1.2 -1.7 -4.8 -3.5 -2.7 -3.0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0.3 -1.4 -0.7 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 4.4 6.5 7.8 8.9 10.2 – 10.3 10.7 10.4 0.8 1.0 3.6 0.3 0 -0.5
Endoplasmic Reticulum -3.0 -6.8 -6.4 0 0 – 0 0 0 1.2 1.1 0.9 -3.5 -4.1 -3.2
Endosome -2.5 2.3 -0.8 0 0 – 0 0 0 -2.3 -0.4 0.5 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria -2.8 -3.6 0.6 0 0 – 0 0 0 -3.0 -3.7 -3.9 -0.4 -0.2 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 2.0 0.8 -2.2 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0.1 0.4 -0.3 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -0.9 1.9 0.8 1.0 1.1 – 2.6 0.4 1.1 0 0 1.8 9.9 9.6 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.0525 4.2213 4.1998 3.6234 3.4248 3.9813 4.8054 6.4902 6.1237 5.6107 6.0095 5.9961 2.7454 4.0586 3.8538 3.2662 3.3877 3.786
Actin 0.072 0.0424 0.0692 0.0571 0.0694 0.0313 0.0813 0.0221 0.0714 0.0356 0.0302 0.0639 0.0542 0.0229 0.0518 0.0396 0.0088 0.0193
Bud 0.0269 0.0265 0.1371 0.0522 0.0291 0.0086 0.0203 0.0188 0.0687 0.0105 0.0036 0.0037 0.0303 0.0165 0.1022 0.0216 0.0185 0.0025
Bud Neck 0.0396 0.0088 0.0075 0.0117 0.0103 0.514 0.0332 0.0165 0.0097 0.003 0.007 0.5015 0.0315 0.0138 0.0069 0.0093 0.0343 0.5666
Bud Periphery 0.057 0.0487 0.3031 0.1738 0.076 0.0186 0.0609 0.0346 0.205 0.1057 0.0128 0.008 0.0771 0.021 0.204 0.1 0.0486 0.0061
Bud Site 0.1616 0.2373 0.0665 0.0273 0.0375 0.0083 0.1246 0.2839 0.0563 0.0026 0.0078 0.0073 0.1017 0.2627 0.0593 0.0115 0.0042 0.0067
Cell Periphery 0.013 0.0045 0.0049 0.0051 0.0115 0.0053 0.0363 0.0087 0.0073 0.0076 0.013 0.0054 0.0187 0.003 0.0048 0.0047 0.0034 0.0045
Cytoplasm 0.1658 0.2754 0.1279 0.1329 0.2074 0.1303 0.2165 0.156 0.1351 0.2668 0.222 0.1094 0.1888 0.149 0.1039 0.1326 0.1828 0.0834
Cytoplasmic Foci 0.0797 0.0525 0.0367 0.0408 0.0894 0.0583 0.0704 0.0647 0.0473 0.0481 0.0826 0.044 0.07 0.0572 0.0404 0.0373 0.0778 0.0457
Eisosomes 0.0006 0.0001 0.0002 0.0002 0.0006 0.0002 0.0012 0.0002 0.0012 0.0001 0.0002 0.0003 0.0005 0.0001 0.0004 0.0003 0.0001 0.0001
Endoplasmic Reticulum 0.0155 0.0188 0.0064 0.0126 0.0106 0.0114 0.026 0.0292 0.0156 0.014 0.0203 0.0138 0.0218 0.0142 0.0111 0.026 0.0191 0.0096
Endosome 0.1517 0.1487 0.1125 0.2486 0.2046 0.1039 0.1356 0.1886 0.1837 0.2443 0.3457 0.1221 0.1933 0.2633 0.2228 0.3484 0.3297 0.1298
Golgi 0.0591 0.0389 0.0434 0.0744 0.0925 0.0428 0.0504 0.0518 0.069 0.1681 0.1178 0.0445 0.0544 0.0466 0.057 0.0572 0.0978 0.052
Lipid Particles 0.0329 0.0112 0.0055 0.0077 0.0111 0.0125 0.0345 0.0169 0.0096 0.0068 0.0225 0.0087 0.0202 0.006 0.0049 0.0286 0.0172 0.0059
Mitochondria 0.0272 0.0071 0.0127 0.0396 0.0311 0.0163 0.0112 0.0066 0.0228 0.0056 0.0086 0.0064 0.0092 0.0106 0.0172 0.0121 0.011 0.0069
None 0.008 0.0123 0.0066 0.0031 0.0121 0.003 0.0158 0.0162 0.0034 0.002 0.0022 0.012 0.0078 0.0047 0.0058 0.0058 0.0045 0.007
Nuclear Periphery 0.0092 0.0029 0.0022 0.0029 0.003 0.0007 0.0036 0.003 0.0042 0.0019 0.0021 0.0006 0.0037 0.0026 0.0027 0.0052 0.0093 0.0013
Nucleolus 0.0014 0.0008 0.0011 0.0006 0.006 0.0005 0.0013 0.0025 0.0008 0.0003 0.0002 0.0008 0.0014 0.0012 0.0011 0.0004 0.0005 0.0005
Nucleus 0.0039 0.0029 0.0032 0.0025 0.0219 0.0016 0.0022 0.0121 0.0019 0.001 0.0012 0.0011 0.0025 0.0027 0.0026 0.0018 0.0022 0.0013
Peroxisomes 0.0144 0.0021 0.0057 0.0018 0.0071 0.0047 0.0093 0.0018 0.0119 0.0011 0.0037 0.0027 0.0032 0.002 0.0022 0.0095 0.0077 0.0032
Punctate Nuclear 0.0034 0.0016 0.002 0.0005 0.004 0.0024 0.0044 0.0047 0.001 0.0003 0.0004 0.0004 0.0024 0.0012 0.0008 0.0019 0.0023 0.0017
Vacuole 0.0509 0.0511 0.0384 0.088 0.0592 0.0219 0.0559 0.0564 0.0617 0.0664 0.0875 0.0383 0.1006 0.0895 0.082 0.1272 0.1044 0.0403
Vacuole Periphery 0.0062 0.0052 0.0072 0.0164 0.0055 0.0034 0.0052 0.0048 0.0121 0.0082 0.0085 0.005 0.0067 0.009 0.0161 0.0189 0.0156 0.0054

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 50.4728 33.0579 18.5635 28.3296 41.467 28.9683 22.8102 24.3886 19.8917 48.1946
Translational Efficiency 1.4383 1.1306 1.3043 0.852 1.1742 2.1978 1.5949 0.8982 1.0371 1.0158

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Transmembrane osmosensor for filamentous growth and HOG pathways; involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol (HOG) response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p
Localization
Cell Percentages ER (14%), cell periphery (7%), mixed (39%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sho1

Sho1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sho1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available