Standard name | |
---|---|
Human Ortholog | |
Description | Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to substrate PI4P in the PM; interacts with FFAT motifs in Opi1p, Swh1p, Osh2p, and Osh3p; involved in telomeric silencing; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | – | 5 | 6 | 19 | 7 | 9 | 11 | – | 14 | 7 | 17 | 12 | 25 | 34 | 38 | 36 | 29 | 1 | 0 | 3 | 2 | 10 | 18 |
Bud | – | – | 1 | 0 | 3 | 4 | 2 | 5 | – | 11 | 7 | 4 | 2 | 3 | 4 | 5 | 4 | 3 | 0 | 0 | 0 | 0 | 2 | 1 |
Bud Neck | – | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Site | – | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | – | 0 | 7 | 9 | 0 | 8 | 6 | – | 8 | 3 | 18 | 9 | 21 | 47 | 43 | 41 | 19 | 0 | 0 | 0 | 1 | 1 | 7 |
Cytoplasm | – | – | 5 | 16 | 32 | 16 | 27 | 21 | – | 25 | 22 | 20 | 19 | 17 | 32 | 40 | 27 | 22 | 0 | 0 | 0 | 0 | 6 | 5 |
Endoplasmic Reticulum | – | – | 43 | 79 | 135 | 76 | 165 | 113 | – | 120 | 94 | 125 | 151 | 164 | 152 | 9 | 2 | 7 | 6 | 26 | 65 | 46 | 138 | 147 |
Endosome | – | – | 0 | 2 | 8 | 0 | 2 | 2 | – | 11 | 0 | 5 | 2 | 1 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 |
Golgi | – | – | 0 | 1 | 0 | 0 | 0 | 1 | – | 2 | 1 | 1 | 0 | 2 | 4 | 52 | 47 | 27 | 0 | 0 | 0 | 1 | 4 | 8 |
Mitochondria | – | – | 12 | 23 | 34 | 20 | 95 | 67 | – | 99 | 132 | 176 | 2 | 1 | 4 | 19 | 11 | 13 | 0 | 1 | 1 | 2 | 5 | 6 |
Nucleus | – | – | 0 | 0 | 2 | 1 | 3 | 2 | – | 1 | 2 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | – | – | 5 | 14 | 11 | 7 | 33 | 26 | – | 30 | 15 | 32 | 2 | 2 | 0 | 5 | 9 | 8 | 0 | 1 | 3 | 2 | 3 | 0 |
Nucleolus | – | – | 0 | 1 | 0 | 0 | 2 | 2 | – | 2 | 4 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | – | 0 | 0 | 0 | 1 | 0 | 0 | – | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | – | – | 1 | 24 | 54 | 9 | 62 | 60 | – | 72 | 74 | 78 | 13 | 30 | 14 | 40 | 31 | 17 | 0 | 0 | 2 | 0 | 3 | 8 |
Unique Cell Count | 57 | 134 | 241 | 112 | 298 | 243 | 314 | 287 | 351 | 183 | 220 | 235 | 200 | 167 | 119 | 11 | 33 | 80 | 59 | 184 | 218 | |||
Labelled Cell Count | 72 | 173 | 307 | 141 | 408 | 316 | 400 | 362 | 482 | 213 | 267 | 296 | 257 | 213 | 147 | 11 | 33 | 80 | 59 | 184 | 218 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | – | 7.3 | 8.5 | 8.6 | 7.9 | 7.5 | 7.6 | – | 7.4 | 7.4 | 7.3 | 10.0 | 10.1 | 9.8 | 13.4 | 14.0 | 13.8 | – | 12.1 | 12.7 |
Std Deviation (1e-4) | – | – | 0.8 | 1.3 | 1.6 | 1.5 | 1.5 | 1.3 | – | 1.5 | 1.3 | 1.4 | 1.3 | 1.4 | 1.6 | 2.8 | 2.9 | 3.0 | – | 2.4 | 2.5 |
Intensity Change (Log2) | – | – | – | 0.22 | 0.24 | 0.12 | 0.05 | 0.07 | – | 0.03 | 0.03 | 0.0 | 0.46 | 0.48 | 0.43 | 0.88 | 0.95 | 0.93 | – | 0.73 | 0.8 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0.6 | 1.1 | 1.8 | 2.2 | 2.5 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3.7 | 3.8 | 4.1 | 3.2 |
Cytoplasm | 0.6 | 0.9 | 1.0 | 0.1 | 0 | – | 0 | 0 | 0 | 0.4 | 0 | 1.0 | 2.0 | 1.4 | 1.7 |
Endoplasmic Reticulum | -2.2 | -2.7 | -1.0 | -2.8 | -3.9 | – | -5.2 | -6.0 | -5.7 | 1.2 | -0.1 | -1.5 | -11.8 | -12.1 | -9.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 4.3 | 4.5 | 3.9 |
Mitochondria | -0.6 | -1.3 | -0.5 | 1.6 | 1.0 | – | 1.6 | 3.5 | 4.1 | 0 | 0 | 0 | -2.4 | -3.1 | -1.8 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.4 | 0 | 0 | 0.5 | 0.4 | – | 0.2 | 0 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.0 | 3.6 | 0 | 3.4 | 3.9 | – | 3.7 | 4.0 | 3.6 | 0 | 2.5 | 0 | 3.3 | 3.1 | 2.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.642 | 11.1904 | 9.9783 | 9.9299 | 8.8132 | 9.8811 | 10.6504 | 12.6851 | 11.7336 | 11.0117 | 10.4666 | 11.9566 | 9.2289 | 10.4267 | 9.7157 | 9.1784 | 8.3527 | 10.1024 |
Actin | 0.0711 | 0.0592 | 0.0592 | 0.0542 | 0.0228 | 0.027 | 0.0513 | 0.0218 | 0.0231 | 0.0017 | 0.0326 | 0.0055 | 0.065 | 0.0416 | 0.03 | 0.0212 | 0.0028 | 0.0323 |
Bud | 0.0016 | 0.0003 | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0004 | 0.0006 | 0.0003 | 0.0001 | 0.0002 | 0.0001 |
Bud Neck | 0.0052 | 0.003 | 0.004 | 0.0149 | 0.0008 | 0.001 | 0.0013 | 0.0005 | 0.001 | 0.0004 | 0.0005 | 0.0015 | 0.0016 | 0.0029 | 0.0015 | 0.003 | 0.0003 | 0.0021 |
Bud Periphery | 0.0044 | 0.0008 | 0.0008 | 0.0004 | 0.0005 | 0.0007 | 0.0016 | 0.0003 | 0.0007 | 0.0005 | 0.0005 | 0.0003 | 0.0014 | 0.0021 | 0.0006 | 0.0003 | 0.0003 | 0.0005 |
Bud Site | 0.0103 | 0.0058 | 0.0056 | 0.0031 | 0.0006 | 0.0002 | 0.0025 | 0.0007 | 0.0009 | 0.0009 | 0.0015 | 0 | 0.0014 | 0.0023 | 0.004 | 0.0002 | 0.0013 | 0.0002 |
Cell Periphery | 0.0215 | 0.0186 | 0.0188 | 0.0036 | 0.0016 | 0.0073 | 0.0108 | 0.0162 | 0.01 | 0.0024 | 0.0011 | 0.0063 | 0.0116 | 0.0164 | 0.008 | 0.0011 | 0.0005 | 0.008 |
Cytoplasm | 0.0248 | 0.0174 | 0.0444 | 0.0044 | 0.0036 | 0.0035 | 0.0296 | 0.0276 | 0.0026 | 0.001 | 0.0019 | 0.0038 | 0.0061 | 0.0075 | 0.0135 | 0.0016 | 0.001 | 0.0044 |
Cytoplasmic Foci | 0.0377 | 0.0074 | 0.0147 | 0.0121 | 0.0078 | 0.0063 | 0.0254 | 0.0026 | 0.0064 | 0.0007 | 0.0102 | 0.003 | 0.0182 | 0.0076 | 0.0066 | 0.0027 | 0.0184 | 0.0027 |
Eisosomes | 0.005 | 0.0053 | 0.006 | 0.001 | 0.0005 | 0.0024 | 0.0088 | 0.0054 | 0.0112 | 0.0009 | 0.0002 | 0.0014 | 0.012 | 0.0061 | 0.0139 | 0.0002 | 0.0001 | 0.0042 |
Endoplasmic Reticulum | 0.5067 | 0.7102 | 0.6658 | 0.817 | 0.6712 | 0.7634 | 0.6685 | 0.8701 | 0.8263 | 0.8891 | 0.792 | 0.8734 | 0.6255 | 0.7289 | 0.8092 | 0.8678 | 0.7851 | 0.8463 |
Endosome | 0.0422 | 0.0145 | 0.0206 | 0.0079 | 0.0256 | 0.0237 | 0.0287 | 0.0051 | 0.0116 | 0.0177 | 0.0213 | 0.0105 | 0.0356 | 0.0207 | 0.0128 | 0.0156 | 0.018 | 0.0103 |
Golgi | 0.0542 | 0.0576 | 0.0884 | 0.0161 | 0.069 | 0.0388 | 0.0641 | 0.0214 | 0.0325 | 0.0133 | 0.0298 | 0.026 | 0.0904 | 0.0554 | 0.0379 | 0.0253 | 0.0089 | 0.0503 |
Lipid Particles | 0.0921 | 0.0729 | 0.0347 | 0.0067 | 0.0437 | 0.0502 | 0.0279 | 0.0168 | 0.0389 | 0.0068 | 0.0197 | 0.0359 | 0.0784 | 0.071 | 0.0337 | 0.0286 | 0.0214 | 0.0103 |
Mitochondria | 0.0056 | 0.007 | 0.0034 | 0.0006 | 0.0336 | 0.0207 | 0.0323 | 0.0004 | 0.0133 | 0.0019 | 0.0212 | 0.0015 | 0.0105 | 0.0049 | 0.0044 | 0.0021 | 0.0069 | 0.0013 |
None | 0.0032 | 0.0005 | 0.0021 | 0.0068 | 0.0017 | 0.001 | 0.0082 | 0.0003 | 0.0003 | 0.0004 | 0.0003 | 0.0002 | 0.007 | 0.0076 | 0.0054 | 0.0002 | 0.0007 | 0.0004 |
Nuclear Periphery | 0.0675 | 0.0136 | 0.0116 | 0.0345 | 0.0724 | 0.0418 | 0.0137 | 0.0077 | 0.0084 | 0.021 | 0.0488 | 0.0257 | 0.0207 | 0.0129 | 0.0085 | 0.0229 | 0.099 | 0.0206 |
Nucleolus | 0.0041 | 0.0001 | 0.0039 | 0.0016 | 0.0023 | 0.0003 | 0.0004 | 0 | 0 | 0.0011 | 0.0001 | 0 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0003 | 0 |
Nucleus | 0.0042 | 0.0004 | 0.0029 | 0.0013 | 0.008 | 0.0027 | 0.0013 | 0.0002 | 0.0002 | 0.0014 | 0.0008 | 0.0004 | 0.0012 | 0.0009 | 0.0006 | 0.0006 | 0.003 | 0.0004 |
Peroxisomes | 0.0101 | 0.0009 | 0.004 | 0.0027 | 0.0086 | 0.0013 | 0.0109 | 0.0001 | 0.0085 | 0.0002 | 0.0112 | 0.0001 | 0.0029 | 0.0005 | 0.0034 | 0.0003 | 0.0198 | 0.0009 |
Punctate Nuclear | 0.015 | 0.0009 | 0.0048 | 0.0068 | 0.0015 | 0.0009 | 0.0056 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0037 | 0.0026 | 0.0021 | 0.0002 | 0.0061 | 0.0009 |
Vacuole | 0.0091 | 0.0026 | 0.0029 | 0.0021 | 0.0095 | 0.0036 | 0.0044 | 0.002 | 0.0022 | 0.0331 | 0.0028 | 0.0022 | 0.0038 | 0.0055 | 0.0018 | 0.0036 | 0.0024 | 0.0022 |
Vacuole Periphery | 0.0044 | 0.0009 | 0.0011 | 0.002 | 0.0143 | 0.0029 | 0.0024 | 0.0006 | 0.0013 | 0.0052 | 0.0028 | 0.0019 | 0.0024 | 0.0019 | 0.0012 | 0.0023 | 0.0037 | 0.0014 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 200.4143 | 240.2195 | 234.0196 | 188.2817 | 201.4535 | 190.5507 | 244.921 | 209.7224 | 189.7857 | 181.4945 |
Translational Efficiency | 2.0498 | 2.0579 | 1.8279 | 1.991 | 2.132 | 2.3202 | 1.7816 | 1.8905 | 2.0287 | 1.9477 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1494 | 1360 | 1780 | 112 | 1947 | 1671 | 2423 | 2266 | 3441 | 3031 | 4203 | 2378 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 887.62 | 1079.57 | 1474.05 | 1745.75 | 1040.26 | 1038.87 | 1842.55 | 1564.32 | 973.99 | 1057.13 | 1686.49 | 1572.87 |
Standard Deviation | 132.19 | 184.25 | 236.01 | 329.76 | 152.09 | 163.22 | 324.02 | 336.05 | 162.48 | 174.15 | 342.44 | 337.95 |
Intensity Change Log 2 | — | 0.282443 | 0.731771 | 0.975833 | — | -0.001929 | 0.824760 | 0.588592 | — | 0.135987 | 0.782690 | 0.779846 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.008759 | 0.012198 | 0.021787 | 0.091145 | 0.015595 | 0.015142 | 0.123570* | 0.158037* | 0.012627 | 0.013821 | 0.080464 | 0.154886* |
Bud Neck | 0.000780 | 0.000946 | 0.004122 | 0.029284 | 0.000690 | 0.000910 | 0.011184 | 0.014170 | 0.000729 | 0.000926 | 0.008193 | 0.014882 |
Bud Site | 0.003994 | 0.006031 | 0.006624 | 0.022393 | 0.004126 | 0.006749 | 0.009999 | 0.022987 | 0.004068 | 0.006427 | 0.008570 | 0.022959 |
Cell Periphery | 0.007156 | 0.010441 | 0.003439 | 0.004034 | 0.011686 | 0.012420 | 0.009560 | 0.009228 | 0.009719 | 0.011532 | 0.006968 | 0.008983 |
Cytoplasm | 0.032614 | 0.019583 | 0.002397 | 0.004142 | 0.015986 | 0.015511 | 0.001199 | 0.001681 | 0.023205 | 0.017339 | 0.001706 | 0.001797 |
Cytoplasmic Foci | 0.008208 | 0.005392 | 0.000909 | 0.017619 | 0.006490 | 0.009289 | 0.001447 | 0.005106 | 0.007236 | 0.007541 | 0.001219 | 0.005695 |
Eisosomes | 0.002311 | 0.002773 | 0.001785 | 0.007010 | 0.003260 | 0.004489 | 0.024836 | 0.012976 | 0.002848 | 0.003719 | 0.015074 | 0.012695 |
Endoplasmic Reticulum | 0.812238* | 0.841971* | 0.855568* | 0.364878* | 0.844778* | 0.807300* | 0.608430* | 0.462487* | 0.830650* | 0.822857* | 0.713094* | 0.457890* |
Endosome | 0.010136 | 0.012324 | 0.021441 | 0.069492 | 0.011741 | 0.014880 | 0.020599 | 0.041763 | 0.011044 | 0.013733 | 0.020956 | 0.043069 |
Golgi | 0.019199 | 0.011393 | 0.002763 | 0.022571 | 0.011303 | 0.015938 | 0.015280 | 0.013936 | 0.014732 | 0.013899 | 0.009979 | 0.014342 |
Lipid Particles | 0.047580 | 0.047312 | 0.062605 | 0.313549* | 0.043359 | 0.052547 | 0.149544* | 0.236807* | 0.045192 | 0.050198 | 0.112725* | 0.240422* |
Mitochondria | 0.005420 | 0.003194 | 0.000532 | 0.000757 | 0.002272 | 0.007328 | 0.003787 | 0.002413 | 0.003639 | 0.005473 | 0.002408 | 0.002335 |
Mitotic Spindle | 0.000225 | 0.000825 | 0.000541 | 0.011528 | 0.000301 | 0.001013 | 0.000577 | 0.000658 | 0.000268 | 0.000929 | 0.000561 | 0.001170 |
None | 0.004045 | 0.003831 | 0.001646 | 0.000042 | 0.004762 | 0.004401 | 0.007111 | 0.001320 | 0.004451 | 0.004145 | 0.004797 | 0.001260 |
Nuclear Periphery | 0.000616 | 0.000575 | 0.001781 | 0.000964 | 0.000482 | 0.000932 | 0.000769 | 0.000592 | 0.000540 | 0.000772 | 0.001198 | 0.000609 |
Nuclear Periphery Foci | 0.009958 | 0.004889 | 0.008237 | 0.018820 | 0.004655 | 0.006037 | 0.000714 | 0.002979 | 0.006957 | 0.005522 | 0.003900 | 0.003725 |
Nucleolus | 0.000217 | 0.000084 | 0.000018 | 0.000021 | 0.000018 | 0.000110 | 0.000021 | 0.000026 | 0.000104 | 0.000099 | 0.000020 | 0.000026 |
Nucleus | 0.001244 | 0.001082 | 0.000209 | 0.000263 | 0.000666 | 0.000978 | 0.000302 | 0.000205 | 0.000917 | 0.001025 | 0.000263 | 0.000208 |
Peroxisomes | 0.023438 | 0.013580 | 0.001012 | 0.001095 | 0.016728 | 0.020225 | 0.008552 | 0.007669 | 0.019641 | 0.017243 | 0.005359 | 0.007359 |
Vacuole | 0.001245 | 0.001040 | 0.002348 | 0.019609 | 0.000657 | 0.001774 | 0.001877 | 0.003632 | 0.000912 | 0.001445 | 0.002076 | 0.004384 |
Vacuole Periphery | 0.000616 | 0.000536 | 0.000238 | 0.000785 | 0.000446 | 0.002024 | 0.000640 | 0.001328 | 0.000520 | 0.001357 | 0.000470 | 0.001303 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.58 | -4.99 | -4.62 | -4.42 | -3.84 | 0.18 | -20.47 | -23.93* | -23.97* | -4.54 | -0.71 | -20.39 | -25.35 | -25.06 | -11.74 |
Bud Neck | -0.73 | -6.61 | -4.20 | -4.18 | -3.69 | -1.39 | -13.07 | -12.91 | -12.66 | -2.29 | -1.48 | -14.56 | -13.54 | -13.31 | -5.74 |
Bud Site | -1.84 | -2.61 | -3.77 | -3.34 | -3.16 | -2.54 | -6.26 | -11.09 | -8.67 | -7.13 | -3.12 | -6.40 | -11.65 | -9.72 | -8.46 |
Cell Periphery | -2.17 | 3.92 | 2.49 | 4.27 | -0.63 | -0.44 | 1.58 | 1.82 | 2.45 | 0.35 | -1.58 | 3.17 | 0.72 | 2.50 | -3.10 |
Cytoplasm | 5.28 | 17.15 | 4.57 | 2.18 | -1.03 | 0.32 | 14.02 | 13.46 | 12.22 | -2.69 | 4.20 | 21.95 | 21.22 | 14.79 | -0.68 |
Cytoplasmic Foci | 2.41 | 7.81 | -1.97 | -2.53 | -3.43 | -2.12 | 6.37 | 1.35 | 3.29 | -5.36 | -0.34 | 9.95 | 1.66 | 1.86 | -6.65 |
Eisosomes | -0.97 | 1.05 | -1.23 | -1.10 | -1.37 | -1.99 | -13.01 | -8.34 | -6.84 | 6.03 | -2.16 | -12.30 | -8.90 | -7.92 | 1.67 |
Endoplasmic Reticulum | -2.97 | -4.37 | 11.94 | 12.72 | 13.07 | 4.05 | 24.49 | 37.48* | 31.10* | 12.82 | 1.16 | 16.64 | 39.93 | 37.81 | 25.78 |
Endosome | -1.96 | -8.52 | -5.93 | -5.70 | -4.75 | -2.70 | -8.44 | -18.18 | -15.15 | -12.40 | -3.30 | -12.01 | -20.14 | -17.85 | -13.31 |
Golgi | 4.44 | 11.91 | -0.53 | -1.95 | -3.59 | -2.90 | -3.36 | -1.94 | 1.33 | 1.22 | 0.69 | 5.28 | 0.38 | -0.29 | -4.39 |
Lipid Particles | 0.07 | -3.12 | -8.31 | -8.30 | -7.79 | -2.16 | -19.40 | -29.00 | -26.53 | -11.24 | -1.62 | -17.62 | -30.86 | -29.39 | -18.56 |
Mitochondria | 1.75 | 4.81 | 4.40 | 2.96 | -0.65 | -4.20 | -3.10 | -0.35 | 3.99 | 2.49 | -2.12 | 2.33 | 2.20 | 3.85 | 0.15 |
Mitotic Spindle | -1.35 | -1.80 | -2.87 | -2.73 | -2.78 | -1.70 | -2.20 | -2.87 | 0.82 | -0.68 | -2.17 | -2.77 | -4.04 | -0.91 | -2.53 |
None | 0.19 | 2.95 | 5.71 | 3.75 | 3.86 | 0.29 | -1.95 | 4.03 | 3.33 | 6.33 | 0.35 | -0.43 | 5.34 | 4.08 | 6.03 |
Nuclear Periphery | 0.14 | -3.50 | -1.82 | -1.27 | 2.25 | -1.70 | -3.59 | -1.12 | 1.23 | 1.87 | -1.18 | -4.44 | -0.80 | 0.75 | 3.72 |
Nuclear Periphery Foci | 3.40 | 0.99 | -1.41 | -2.16 | -1.65 | -1.40 | 6.51 | 2.10 | 3.19 | -4.35 | 1.67 | 3.78 | 3.55 | 2.14 | 0.20 |
Nucleolus | 0.74 | 1.18 | 1.16 | 1.13 | -0.23 | -1.92 | -0.78 | -1.40 | 1.72 | -1.03 | 0.08 | 1.15 | 1.06 | 1.96 | -0.89 |
Nucleus | 0.52 | 3.41 | 2.69 | 2.68 | -0.87 | -0.84 | 2.72 | 3.40 | 2.21 | 4.40 | -0.34 | 4.29 | 4.64 | 3.73 | 2.00 |
Peroxisomes | 3.93 | 10.94 | 10.99 | 8.21 | -0.17 | -1.62 | 5.25 | 5.88 | 6.63 | 0.74 | 1.47 | 11.35 | 8.84 | 7.07 | -2.10 |
Vacuole | 0.73 | -3.08 | -3.64 | -3.68 | -3.42 | -2.89 | -3.35 | -7.11 | -3.43 | -3.32 | -2.03 | -4.49 | -7.28 | -5.79 | -4.47 |
Vacuole Periphery | 0.44 | 2.74 | -0.66 | -0.91 | -2.26 | -3.07 | -1.57 | -2.14 | 1.06 | -1.65 | -2.83 | 0.50 | -2.00 | 0.10 | -2.13 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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