Standard name
Human Ortholog
Description ADP-ribosylation factor GTPase activating protein (ARF GAP); involved in ER-Golgi transport; shares functional similarity with Gcs1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.16 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0 0.1 0.09 0.11 0 0 0 0 0 0.05
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.08 0.05 0.08 0.12 0 0.05 0 0.06 0 0 0 0 0 0.05 0.05 0.06 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.12 0.06 0.05 0 0.05 0 0 0.07 0 0 0 0.06 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0
Golgi 0.43 0.73 0.42 0.17 0.22 0.06 0 0 0 0 0 0 0.76 0.67 0.68 0.76 0.89 0.88 0.85 0.78 0.79 0.89 0.89 0.81
Mitochondria 0.83 0.36 0.81 0.85 0.72 0.91 0.91 0.91 0.9 0.9 0.93 0.95 0.56 0.55 0.43 0.1 0.11 0.08 0 0 0.05 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.17 0.12 0.06 0.08 0.12 0 0.05 0.08 0 0.05 0 0 0.11 0.13 0.13 0.19 0.16 0.16 0 0.11 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 8 54 7 0 1 5 0 4 6 2 2 3 15 14 4 31 23 20 2 6 5 3 8 14
Bud 3 0 1 1 0 0 0 4 3 3 3 6 1 4 3 0 1 1 0 1 2 0 3 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 2 0 0 2 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 2 3 2 0 5 0 1 2 2 2 2 0 0 0 0 0 0
Cytoplasm 2 25 8 8 14 8 9 6 12 6 7 4 6 9 16 15 16 10 1 4 1 0 1 0
Endoplasmic Reticulum 4 41 9 5 1 10 8 10 13 3 6 10 22 13 15 6 7 9 1 3 1 1 2 4
Endosome 0 0 1 0 1 0 1 0 0 0 0 0 3 3 2 10 4 0 7 7 10 4 4 8
Golgi 60 242 65 17 25 11 6 12 7 8 5 5 266 194 237 237 223 168 134 275 137 190 276 218
Mitochondria 115 119 126 84 81 172 175 253 166 176 172 220 195 158 150 30 27 16 4 11 8 5 7 6
Nucleus 0 0 0 0 1 1 0 1 0 3 0 0 1 0 2 2 3 0 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 1 1 3 3 3 2 4 3 0 0 0 2 2 0 0 0 0 1 0 0
Nucleolus 3 3 3 4 2 4 6 12 6 6 5 5 4 0 3 7 4 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 24 39 9 8 13 8 9 21 4 10 5 4 38 38 47 60 39 31 5 38 2 3 5 6
Unique Cell Count 138 333 155 99 112 189 193 277 185 196 185 231 348 289 349 313 251 190 158 354 174 214 311 268
Labelled Cell Count 219 524 229 127 140 221 217 328 226 222 209 267 551 434 481 403 351 258 158 354 174 214 311 268


Early Golgi, Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.7 20.1 13.0 11.2 11.6 11.2 9.3 9.4 9.6 9.4 9.2 9.0 16.8 16.1 16.4 17.2 22.3 23.2 14.6 14.6 14.7
Std Deviation (1e-4) 2.4 3.5 3.0 2.9 2.7 2.7 2.1 2.3 2.2 2.1 2.1 2.2 3.1 3.6 3.6 5.0 6.2 5.8 3.0 2.3 2.6
Intensity Change (Log2) – – – -0.22 -0.17 -0.22 -0.48 -0.47 -0.44 -0.47 -0.5 -0.54 0.37 0.31 0.33 0.4 0.78 0.83 0.17 0.16 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 -0.9 0 0 -0.6 0 0 0 -0.1 0.2 0 2.0 1.7 2.1
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 2.2 -0.4 -0.2 -1.7 0.5 -1.0 -0.6 0 0 -1.1 -0.3 -0.2 0.5 0
Endoplasmic Reticulum -0.3 0 -0.2 -0.7 -1.1 0.5 -2.2 -1.1 -0.7 0.2 -0.6 -0.7 0 -1.5 -0.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -4.1 -3.3 -8.0 -8.9 -9.8 -8.6 -8.7 -8.9 -9.9 7.5 5.1 5.5 7.2 10.1 9.2
Mitochondria 0.7 -1.7 2.6 2.5 3.1 2.2 2.3 3.3 4.4 -5.4 -5.6 -8.0 -15.5 -14.2 -13.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1.3 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.7 1.7 -0.7 -0.5 0.7 -1.7 -0.3 -1.4 -2.2 1.8 2.4 2.5 3.8 3.0 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 20.3041 22.1351 21.1867 21.2803 21.072 20.912 16.6525 19.0228 18.403 18.9213 17.201 18.0947 20.7849 21.9109 21.4243 21.687 21.3409 21.2052
Actin 0.0018 0.0009 0.0006 0.0014 0.0004 0.0023 0.0233 0.0042 0.0175 0.0098 0.0054 0.0046 0.0055 0.0017 0.0071 0.0064 0.0121 0.0014
Bud 0.0001 0 0.0001 0.0002 0 0 0.0003 0.0001 0.0003 0.0001 0.0001 0.0001 0.0003 0.0001 0.0006 0.0004 0.0003 0.0001
Bud Neck 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001
Bud Periphery 0.0001 0 0 0.0003 0.0001 0.0001 0.0003 0.0001 0.0003 0.0001 0.0001 0.0001 0.0003 0.0001 0.0007 0.0014 0.0006 0.0001
Bud Site 0.0003 0.0001 0 0.0001 0 0 0.0007 0.0001 0.0002 0.0002 0.0002 0.0001 0.0005 0.0001 0.0003 0.0002 0.0002 0
Cell Periphery 0.0002 0.0001 0 0.0001 0 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0.0001 0.0001 0.0008 0.0002 0
Cytoplasm 0.0008 0.0018 0.0009 0.0004 0.0005 0.0004 0.0069 0.0026 0.0028 0.0008 0.0006 0.0005 0.0013 0.002 0.0013 0.0011 0.0007 0.0007
Cytoplasmic Foci 0.0117 0.0194 0.0118 0.0091 0.0095 0.0074 0.0216 0.024 0.017 0.0151 0.0165 0.0095 0.0121 0.0223 0.0143 0.0139 0.0131 0.0102
Eisosomes 0 0 0 0.0001 0 0.0001 0.0002 0 0.0001 0.0001 0 0 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0007 0.0007 0.0007 0.0007 0.0007 0.0007 0.0027 0.0009 0.0023 0.0006 0.0009 0.0004 0.001 0.0008 0.0008 0.0014 0.0009 0.0007
Endosome 0.1194 0.1598 0.1299 0.0891 0.0998 0.0738 0.1161 0.1465 0.1163 0.125 0.1288 0.0699 0.1395 0.1731 0.157 0.2251 0.1893 0.0955
Golgi 0.7982 0.7645 0.7922 0.8321 0.8522 0.8708 0.7336 0.7883 0.8125 0.7915 0.7842 0.8799 0.7401 0.7684 0.7728 0.6866 0.7654 0.8608
Lipid Particles 0.004 0.0013 0.0005 0.0011 0.0006 0.0014 0.0043 0.0008 0.001 0.0005 0.0143 0.0003 0.0051 0.001 0.0015 0.0025 0.0029 0.0009
Mitochondria 0.0528 0.0415 0.0494 0.0445 0.0318 0.0342 0.0703 0.0214 0.0184 0.0129 0.0139 0.0323 0.0794 0.0199 0.0247 0.023 0.0047 0.0235
None 0 0 0 0 0 0 0.0032 0 0.0004 0 0 0 0 0 0.0001 0.0001 0 0
Nuclear Periphery 0.0001 0.0001 0.0002 0.0003 0.0001 0.0004 0.0027 0.0003 0.0003 0.0004 0.0004 0 0.0003 0.0001 0.0003 0.001 0.0005 0.0001
Nucleolus 0.0002 0 0 0 0 0 0.0002 0 0 0 0 0 0 0 0.0001 0.0001 0 0
Nucleus 0.0001 0 0 0.0001 0 0 0.0028 0.0001 0.0001 0 0 0 0.0001 0 0.0001 0.0002 0 0
Peroxisomes 0.0003 0.0001 0.0001 0.0001 0.0001 0 0.0026 0.0004 0.001 0.0002 0.023 0.0003 0.0002 0.0002 0.0004 0.0001 0.0001 0.0001
Punctate Nuclear 0 0 0 0 0 0 0.0015 0 0.0002 0 0.0001 0 0 0 0.0001 0 0 0
Vacuole 0.0038 0.004 0.0038 0.0085 0.001 0.0027 0.0019 0.0046 0.0039 0.0137 0.0019 0.0005 0.0044 0.003 0.0076 0.0149 0.0023 0.0015
Vacuole Periphery 0.0051 0.0054 0.0098 0.0118 0.003 0.0054 0.0043 0.0054 0.0053 0.0288 0.0092 0.0015 0.0094 0.007 0.0098 0.0208 0.0064 0.0042

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 92.3226 85.1931 47.9066 69.0975 76.2607 64.7389 86.3069 82.8918 70.6526 84.4312
Translational Efficiency 2.3591 2.6349 2.8661 1.5257 2.1777 3.0537 1.9535 1.577 1.8041 1.8601

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
62 809 1546 1707 1716 1730 316 302 1778 2539 1862 2009

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1321.60 1255.90 1885.59 1904.07 1359.42 1415.87 1932.73 1874.60 1358.10 1364.90 1893.59 1899.64
Standard Deviation 186.27 337.99 293.70 301.79 190.48 238.92 234.82 331.05 190.46 284.34 285.11 306.54
Intensity Change Log 2 — -0.073564 0.512730 0.526801 — 0.058698 0.507649 0.463592 — -0.004986 0.510156 0.495097

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.003241 0.005101 0.002446 0.001297 0.001027 0.001319 0.002170 0.000898 0.001105 0.002524 0.002399 0.001237
Bud Neck 0.000341 0.000844 0.000597 0.000243 0.000083 0.000201 0.001418 0.000851 0.000092 0.000406 0.000736 0.000334
Bud Site 0.000863 0.003046 0.000637 0.002901 0.000338 0.001675 0.001798 0.007489 0.000356 0.002112 0.000834 0.003590
Cell Periphery 0.000415 0.000531 0.000231 0.000399 0.000117 0.000142 0.000324 0.000512 0.000128 0.000266 0.000247 0.000416
Cytoplasm 0.000341 0.001673 0.000095 0.001396 0.000191 0.000597 0.000190 0.002034 0.000196 0.000940 0.000111 0.001492
Cytoplasmic Foci 0.096420 0.049074 0.010901 0.013730 0.060282 0.061409 0.023160 0.032013 0.061542 0.057479 0.012981 0.016478
Eisosomes 0.000094 0.000088 0.000053 0.000026 0.000029 0.000024 0.000038 0.000023 0.000031 0.000044 0.000050 0.000026
Endoplasmic Reticulum 0.005330 0.004170 0.003032 0.001234 0.002080 0.001309 0.003695 0.002886 0.002194 0.002221 0.003145 0.001482
Endosome 0.054337 0.072776 0.011557 0.020069 0.018610 0.040710 0.041014 0.062871 0.019855 0.050927 0.016556 0.026503
Golgi 0.768180* 0.757992* 0.867189* 0.852460* 0.832634* 0.768416* 0.774480* 0.737217* 0.830386* 0.765095* 0.851455* 0.835136*
Lipid Particles 0.007602 0.023767 0.016401 0.001703 0.002774 0.003131 0.010523 0.009956 0.002942 0.009706 0.015403 0.002943
Mitochondria 0.026577 0.027676 0.005291 0.007074 0.034933 0.037719 0.002771 0.001929 0.034642 0.034519 0.004864 0.006301
Mitotic Spindle 0.000209 0.001091 0.000893 0.002447 0.000877 0.002995 0.002256 0.005821 0.000854 0.002388 0.001124 0.002954
None 0.000242 0.000746 0.000180 0.000104 0.000215 0.000461 0.000082 0.000047 0.000216 0.000552 0.000163 0.000096
Nuclear Periphery 0.000997 0.000352 0.001267 0.000165 0.000234 0.000317 0.003114 0.000506 0.000260 0.000328 0.001580 0.000217
Nuclear Periphery Foci 0.000905 0.001908 0.002482 0.000945 0.000552 0.000742 0.009930 0.003550 0.000564 0.001113 0.003746 0.001337
Nucleolus 0.001561 0.000626 0.000014 0.000024 0.000005 0.000030 0.000014 0.000032 0.000060 0.000220 0.000014 0.000025
Nucleus 0.007213 0.000871 0.000021 0.000062 0.000021 0.000030 0.000023 0.000111 0.000272 0.000298 0.000021 0.000070
Peroxisomes 0.000380 0.000611 0.000043 0.000070 0.000108 0.000297 0.000177 0.002047 0.000117 0.000397 0.000066 0.000367
Vacuole 0.006228 0.017015 0.018537 0.037509 0.007047 0.022558 0.060465 0.088648 0.007018 0.020792 0.025653 0.045197
Vacuole Periphery 0.018526 0.030044 0.058132 0.056141 0.037844 0.055918 0.062358 0.040560 0.037170 0.047674 0.058850 0.053799

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.20 0.66 1.69 3.08 1.63 -0.54 -1.15 0.37 0.74 1.25 -2.63 -2.45 -0.49 2.07 1.89
Bud Neck -3.14 -1.83 0.80 4.19 2.90 -3.77 -3.79 -4.63 -3.89 1.40 -6.61 -6.06 -4.52 0.85 3.30
Bud Site -3.78 0.94 -4.63 0.04 -5.65 -3.03 -3.65 -3.17 -2.55 -2.51 -5.06 -4.63 -6.79 -2.67 -5.71
Cell Periphery -0.61 1.03 0.07 1.64 -3.95 -1.72 -2.70 -3.37 -3.14 -1.34 -5.46 -5.89 -7.35 -3.35 -4.02
Cytoplasm -2.17 2.02 -3.85 0.38 -5.18 -2.95 -0.02 -1.60 -1.42 -1.60 -3.47 4.20 -3.92 -1.86 -4.12
Cytoplasmic Foci 3.83 7.02 6.78 15.55 -3.57 -0.43 13.14 7.81 7.99 -2.36 1.73 25.48 23.21 24.81 -3.87
Eisosomes 0.20 1.70 2.89 6.18 3.60 2.19 -0.85 0.84 0.08 1.19 -3.62 -3.27 1.46 4.05 3.78
Endoplasmic Reticulum 0.70 1.48 2.66 4.37 5.80 2.65 -1.42 -0.59 -1.15 0.47 -0.09 -2.76 2.24 2.07 4.38
Endosome -1.92 4.92 3.92 12.42 -7.87 -13.97 -5.81 -7.64 -3.74 -3.16 -16.94 2.78 -4.67 11.93 -6.76
Golgi 0.34 -3.40 -2.84 -8.84 2.36 8.89 3.93 5.85 2.05 1.91 9.93 -3.27 -0.43 -9.79 2.66
Lipid Particles -3.50 -2.24 1.61 7.73 9.66 -0.55 -2.54 -2.36 -2.23 0.14 -6.28 -8.79 -0.04 6.11 8.65
Mitochondria -0.09 1.73 1.58 5.80 -1.52 -0.77 12.59 13.02 13.09 0.85 0.04 12.13 11.12 12.39 -1.44
Mitotic Spindle -2.07 -1.94 -4.74 -2.26 -2.75 -2.98 -2.59 -3.23 -1.74 -2.27 -2.88 -0.71 -4.10 -0.98 -3.43
None -1.76 0.51 1.11 2.47 5.13 -0.77 8.19 5.91 1.24 0.72 -1.45 3.31 7.64 1.95 5.01
Nuclear Periphery 0.75 -0.31 0.97 1.94 5.97 -1.88 -3.16 -2.77 -1.87 2.83 -1.26 -6.00 0.88 2.31 6.23
Nuclear Periphery Foci -1.55 -2.19 -0.10 1.94 2.61 -1.15 -2.69 -2.68 -2.51 1.70 -2.94 -4.26 -2.91 -0.82 3.02
Nucleolus 0.57 0.99 0.99 1.11 -0.94 -2.13 -4.20 -1.87 -0.75 -1.51 -0.89 0.84 0.56 1.11 -1.41
Nucleus 0.88 1.00 0.99 1.34 -0.96 -1.11 -0.24 -1.01 -0.99 -1.01 -0.09 1.00 0.53 0.80 -1.34
Peroxisomes -1.05 1.84 1.69 4.29 -2.11 -2.07 -1.20 -1.14 -1.03 -1.10 -3.60 1.52 -0.97 0.12 -1.18
Vacuole -4.30 -6.03 -11.49 -7.38 -7.92 -11.33 -9.73 -9.69 -7.82 -2.93 -11.83 -12.73 -16.50 -9.98 -7.57
Vacuole Periphery -2.22 -7.51 -7.25 -8.01 0.56 -6.06 -4.15 -0.67 3.59 3.28 -4.23 -7.65 -6.29 -2.26 1.63
Standard name
Human Ortholog
Description ADP-ribosylation factor GTPase activating protein (ARF GAP); involved in ER-Golgi transport; shares functional similarity with Gcs1p
Localization
Cell Percentages Golgi (13%), nucleus (9%), mixed (47%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Glo3

Glo3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Glo3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available