| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Type 1 S/T protein phosphatase (PP1) catalytic subunit; involved in glycogen metabolism, sporulation and mitotic progression; interacts with multiple regulatory subunits; regulates actomyosin ring formation; subunit of CPF; recruited to mating projections by Afr1p interaction; regulates nucleocytoplasmic shuttling of Hxk2p; import into the nucleus is inhibited during spindle assembly checkpoint arrest; involved in dephosphorylating Rps6a/b and Bnr1p |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 12 | 2 | 4 | 1 | 6 | 0 | 0 | 0 | 1 |
| Bud | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 3 | 1 | 0 | 0 | 10 | 0 | 0 | 0 | 3 |
| Bud Neck | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 |
| Bud Site | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 5 | 6 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
| Cytoplasm | – | 12 | – | – | – | – | – | – | – | – | – | – | – | – | – | 148 | 90 | 98 | 5 | 10 | 1 | 1 | 2 | 3 |
| Endoplasmic Reticulum | – | 4 | – | – | – | – | – | – | – | – | – | – | – | – | – | 21 | 7 | 11 | 8 | 5 | 0 | 0 | 1 | 4 |
| Endosome | – | 3 | – | – | – | – | – | – | – | – | – | – | – | – | – | 15 | 4 | 2 | 0 | 9 | 0 | 0 | 0 | 0 |
| Golgi | – | 4 | – | – | – | – | – | – | – | – | – | – | – | – | – | 162 | 148 | 81 | 0 | 1 | 0 | 0 | 0 | 2 |
| Mitochondria | – | 1 | – | – | – | – | – | – | – | – | – | – | – | – | – | 14 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| Nucleus | – | 4 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 1 | 0 | 1 | 16 | 0 | 0 | 0 | 0 |
| Nuclear Periphery | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 1 | 4 | 0 | 0 | 0 | 0 |
| Nucleolus | – | 17 | – | – | – | – | – | – | – | – | – | – | – | – | – | 29 | 20 | 10 | 0 | 5 | 0 | 0 | 0 | 0 |
| Peroxisomes | – | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| SpindlePole | – | 2 | – | – | – | – | – | – | – | – | – | – | – | – | – | 25 | 22 | 7 | 3 | 25 | 0 | 0 | 0 | 0 |
| Vac/Vac Membrane | – | 39 | – | – | – | – | – | – | – | – | – | – | – | – | – | 131 | 119 | 56 | 3 | 21 | 0 | 0 | 0 | 3 |
| Unique Cell Count | 76 | 490 | 354 | 249 | 31 | 124 | 4 | 3 | 9 | 24 | ||||||||||||||
| Labelled Cell Count | 86 | 565 | 420 | 275 | 31 | 124 | 4 | 3 | 9 | 24 | ||||||||||||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | – | 27.2 | – | – | – | – | – | – | – | – | – | – | – | – | – | 27.4 | 31.4 | 32.4 | – | – | – |
| Std Deviation (1e-4) | – | 6.8 | – | – | – | – | – | – | – | – | – | – | – | – | – | 6.3 | 7.8 | 6.7 | – | – | – |
| Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
Images
Protein Concentration and Protein Localization Data
| R1 | R2 | R3 | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Concentration | 35.1231 | 51.8103 | 42.1289 | 44.3921 | 50.386 | 44.2456 | 34.7721 | 43.9227 | 42.8232 | 44.5877 | 43.6923 | 40.1673 | 33.5921 | 35.9382 | 35.3928 | 34.8946 | 33.286 | 33.7285 |
| Actin | 0.0235 | 0.002 | 0.0179 | 0.0022 | 0.001 | 0.0208 | 0.0219 | 0.0009 | 0.0092 | 0.0003 | 0.0009 | 0.0264 | 0.0008 | 0.0006 | 0.0035 | 0.0017 | 0.0014 | 0.0073 |
| Bud | 0.0009 | 0.0013 | 0.0011 | 0.0007 | 0.0002 | 0.0018 | 0.0012 | 0.0008 | 0.0013 | 0.0007 | 0.0002 | 0.0008 | 0.0004 | 0.0006 | 0.0006 | 0.0005 | 0.0002 | 0.0009 |
| Bud Neck | 0.0011 | 0.0026 | 0.0057 | 0.0021 | 0.0006 | 0.1085 | 0.0052 | 0.0047 | 0.0124 | 0.0025 | 0.005 | 0.0683 | 0.005 | 0.0028 | 0.0043 | 0.0042 | 0.0066 | 0.117 |
| Bud Periphery | 0.0011 | 0.0009 | 0.0017 | 0.0006 | 0.0001 | 0.003 | 0.0014 | 0.001 | 0.001 | 0.0005 | 0.0003 | 0.0021 | 0.0007 | 0.0006 | 0.001 | 0.0005 | 0.0003 | 0.0021 |
| Bud Site | 0.003 | 0.0071 | 0.004 | 0.0008 | 0.0002 | 0.0014 | 0.0326 | 0.0047 | 0.0047 | 0.0006 | 0.0003 | 0.0006 | 0.0016 | 0.0034 | 0.003 | 0.0009 | 0.0003 | 0.001 |
| Cell Periphery | 0.0008 | 0.0023 | 0.0046 | 0.0034 | 0.0002 | 0.0016 | 0.0052 | 0.005 | 0.0028 | 0.0012 | 0.0023 | 0.0056 | 0.0024 | 0.0029 | 0.0057 | 0.001 | 0.0003 | 0.0024 |
| Cytoplasm | 0.0499 | 0.0288 | 0.0508 | 0.0675 | 0.0196 | 0.0258 | 0.0775 | 0.033 | 0.0398 | 0.0467 | 0.0535 | 0.0323 | 0.0172 | 0.0295 | 0.01 | 0.0299 | 0.0435 | 0.0152 |
| Cytoplasmic Foci | 0.0596 | 0.0361 | 0.0395 | 0.0253 | 0.0464 | 0.0422 | 0.0082 | 0.0125 | 0.0141 | 0.0009 | 0.0009 | 0.0122 | 0.0164 | 0.0351 | 0.0212 | 0.0176 | 0.0439 | 0.0448 |
| Eisosomes | 0.0004 | 0.0001 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0012 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 |
| Endoplasmic Reticulum | 0.0058 | 0.0012 | 0.0115 | 0.0095 | 0.0008 | 0.006 | 0.0069 | 0.0017 | 0.0013 | 0.0007 | 0.0074 | 0.0073 | 0.002 | 0.0013 | 0.0043 | 0.002 | 0.0035 | 0.0067 |
| Endosome | 0.0132 | 0.0024 | 0.0131 | 0.0093 | 0.0041 | 0.0098 | 0.0066 | 0.0009 | 0.0024 | 0.0023 | 0.007 | 0.0049 | 0.0031 | 0.0024 | 0.0038 | 0.0056 | 0.0044 | 0.0106 |
| Golgi | 0.0052 | 0.0007 | 0.0041 | 0.0008 | 0.0039 | 0.0042 | 0.0017 | 0.0003 | 0.0006 | 0.0001 | 0.0002 | 0.0021 | 0.0013 | 0.0007 | 0.0017 | 0.0069 | 0.0021 | 0.0049 |
| Lipid Particles | 0.0289 | 0.0153 | 0.0084 | 0.0222 | 0.0316 | 0.0303 | 0.0033 | 0.0098 | 0.011 | 0.0007 | 0.0005 | 0.0173 | 0.0173 | 0.0196 | 0.0307 | 0.0227 | 0.0381 | 0.0518 |
| Mitochondria | 0.0078 | 0.0016 | 0.0082 | 0.0043 | 0.0328 | 0.0032 | 0.0053 | 0.0006 | 0.0027 | 0.0003 | 0.0008 | 0.0039 | 0.0011 | 0.0005 | 0.001 | 0.0011 | 0.0004 | 0.001 |
| None | 0.0101 | 0.0006 | 0.0068 | 0.0065 | 0.0078 | 0.0038 | 0.0072 | 0.0003 | 0.0007 | 0.0003 | 0.0002 | 0.0009 | 0.0006 | 0.0003 | 0.0003 | 0.0004 | 0.0003 | 0.0004 |
| Nuclear Periphery | 0.0655 | 0.0264 | 0.0775 | 0.0786 | 0.1036 | 0.0759 | 0.0651 | 0.0424 | 0.0429 | 0.0334 | 0.094 | 0.091 | 0.101 | 0.0501 | 0.0988 | 0.1019 | 0.1397 | 0.0922 |
| Nucleolus | 0.1654 | 0.2943 | 0.189 | 0.2496 | 0.1239 | 0.1043 | 0.1846 | 0.2483 | 0.342 | 0.0888 | 0.1627 | 0.1253 | 0.1517 | 0.215 | 0.2419 | 0.1173 | 0.0391 | 0.1082 |
| Nucleus | 0.4053 | 0.4348 | 0.4541 | 0.427 | 0.4958 | 0.4096 | 0.4489 | 0.5536 | 0.4357 | 0.7811 | 0.5862 | 0.4326 | 0.563 | 0.4825 | 0.4529 | 0.476 | 0.4621 | 0.3492 |
| Peroxisomes | 0.0119 | 0.0004 | 0.0023 | 0.0004 | 0.0047 | 0.0014 | 0.0007 | 0.0002 | 0.0005 | 0 | 0 | 0.0003 | 0.0005 | 0.0003 | 0.0005 | 0.0004 | 0.0003 | 0.0011 |
| Punctate Nuclear | 0.1325 | 0.129 | 0.0764 | 0.0705 | 0.1174 | 0.1323 | 0.105 | 0.068 | 0.0614 | 0.0041 | 0.0644 | 0.1445 | 0.105 | 0.1373 | 0.0994 | 0.2045 | 0.2089 | 0.1701 |
| Vacuole | 0.0047 | 0.0107 | 0.0187 | 0.0121 | 0.0016 | 0.0103 | 0.0073 | 0.0101 | 0.0107 | 0.0255 | 0.0104 | 0.0177 | 0.0071 | 0.012 | 0.0125 | 0.0032 | 0.0031 | 0.0108 |
| Vacuole Periphery | 0.0034 | 0.0016 | 0.004 | 0.0063 | 0.0037 | 0.0038 | 0.0029 | 0.0011 | 0.0027 | 0.0095 | 0.0027 | 0.0037 | 0.0017 | 0.0025 | 0.0027 | 0.0014 | 0.0015 | 0.0023 |
Sequencing Data
| R1 | R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Gene Expression | 125.1465 | 136.559 | 120.8197 | 164.1101 | 123.4074 | 95.1292 | 139.4023 | 160.9338 | 142.3801 | 144.3759 |
| Translational Efficiency | 2.1153 | 2.0117 | 1.933 | 1.2856 | 1.595 | 2.6344 | 2.1454 | 1.3056 | 1.5478 | 1.5561 |
Hit Data
| Dataset | Hit |
|---|---|
| Protein Concentration | ✔ |
| Protein Localization | ✘ |
| Gene Expression | ✔ |
| Translational Efficiency | ✘ |
| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Type 1 S/T protein phosphatase (PP1) catalytic subunit; involved in glycogen metabolism, sporulation and mitotic progression; interacts with multiple regulatory subunits; regulates actomyosin ring formation; subunit of CPF; recruited to mating projections by Afr1p interaction; regulates nucleocytoplasmic shuttling of Hxk2p; import into the nucleus is inhibited during spindle assembly checkpoint arrest; involved in dephosphorylating Rps6a/b and Bnr1p |
| Localization | |
| Cell Percentages | cytoplasm (24%), nucleus (3%), mixed (56%) |
| Cell Cycle Regulation | No |
| Subcompartmental Group | N/A |
Glc7
Glc7
Nearest neighbours ( genes)
correlation threshold
0.80
Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Glc7-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
