Standard name | |
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Human Ortholog | |
Description | Ubiquitin-specific protease involved in transport and osmotic response; negatively regulates Ras/PKA signaling; interacts with Bre5p to coregulate anterograde, retrograde transport between ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; role in ribophagy; cleaves ubiquitin fusions but not polyubiquitin; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 1 | 1 | 2 | 10 | 12 | 14 | 7 | 11 | 17 | 18 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 1 | 3 | 1 |
Bud | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 2 | 2 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 1 | 4 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 19 | 22 | 17 | 14 | 24 | 15 | 18 | 10 | 12 | 17 | 14 | 6 | 8 | 11 | 17 | 31 | 12 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 296 | 498 | 439 | 218 | 278 | 269 | 247 | 230 | 153 | 159 | 151 | 136 | 271 | 290 | 327 | 369 | 314 | 342 | 277 | 501 | 438 | 147 | 245 | 155 |
Endoplasmic Reticulum | 8 | 26 | 37 | 27 | 33 | 59 | 60 | 79 | 42 | 46 | 39 | 38 | 5 | 13 | 15 | 13 | 12 | 12 | 9 | 7 | 25 | 35 | 97 | 73 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 2 | 6 |
Golgi | 0 | 0 | 1 | 1 | 1 | 3 | 5 | 8 | 2 | 1 | 0 | 4 | 0 | 0 | 1 | 8 | 2 | 1 | 0 | 0 | 3 | 2 | 12 | 10 |
Mitochondria | 0 | 1 | 0 | 1 | 2 | 15 | 42 | 15 | 37 | 63 | 87 | 57 | 2 | 1 | 0 | 5 | 0 | 2 | 0 | 2 | 6 | 3 | 9 | 4 |
Nucleus | 0 | 0 | 2 | 0 | 1 | 0 | 3 | 5 | 5 | 3 | 2 | 3 | 2 | 1 | 2 | 1 | 5 | 4 | 1 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Vac/Vac Membrane | 2 | 2 | 3 | 1 | 6 | 5 | 13 | 16 | 11 | 15 | 8 | 25 | 0 | 0 | 1 | 48 | 13 | 17 | 2 | 9 | 15 | 8 | 17 | 21 |
Unique Cell Count | 305 | 522 | 476 | 250 | 318 | 346 | 353 | 351 | 231 | 272 | 273 | 246 | 280 | 305 | 343 | 420 | 342 | 369 | 300 | 531 | 497 | 207 | 401 | 285 |
Labelled Cell Count | 310 | 547 | 506 | 266 | 338 | 385 | 400 | 389 | 270 | 311 | 324 | 297 | 286 | 313 | 358 | 464 | 380 | 391 | 300 | 531 | 497 | 207 | 401 | 285 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 15.8 | 17.1 | 13.5 | 12.5 | 13.5 | 11.8 | 11.7 | 12.0 | 11.0 | 10.2 | 9.2 | 10.0 | 15.3 | 16.3 | 16.4 | 16.4 | 18.0 | 17.1 | 14.1 | 15.1 | 16.0 |
Std Deviation (1e-4) | 3.0 | 2.8 | 1.8 | 1.7 | 1.6 | 1.9 | 1.9 | 1.8 | 2.0 | 1.8 | 1.9 | 1.9 | 2.4 | 2.3 | 2.6 | 4.0 | 3.7 | 3.8 | 2.8 | 2.5 | 3.0 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.0 | -0.19 | -0.2 | -0.17 | -0.29 | -0.4 | -0.55 | -0.43 | 0.19 | 0.27 | 0.29 | 0.28 | 0.42 | 0.35 | 0.07 | 0.17 | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 3.7 | 4.0 | 0 | 0 | 5.2 | 5.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.2 | -0.1 | 1.4 | -0.3 | 0.3 | -0.2 | -0.1 | 1.0 | 0.6 | -1.7 | -1.4 | -1.0 | -0.4 | 2.5 | -1.0 |
Cytoplasm | -2.2 | -2.2 | -5.9 | -8.4 | -9.7 | -8.8 | -11.1 | -11.9 | -11.7 | 2.5 | 1.6 | 1.8 | -2.2 | -0.2 | 0.2 |
Endoplasmic Reticulum | 1.4 | 1.3 | 4.1 | 4.1 | 6.0 | 4.1 | 3.8 | 2.8 | 3.2 | -3.5 | -2.0 | -2.0 | -3.0 | -2.5 | -2.8 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.6 | 7.7 | 4.6 | 9.0 | 11.0 | 13.1 | 10.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 3.2 | 3.7 | 0 | 4.2 | 0 | 6.3 | 0 | 0 | 0 | 7.0 | 3.2 | 3.8 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 11.1607 | 15.734 | 13.4232 | 11.5687 | 11.1686 | 13.6283 | 11.7596 | 16.1865 | 14.7565 | 12.2784 | 12.6342 | 14.516 | 10.8645 | 14.3946 | 13.2516 | 13.2177 | 11.5316 | 13.0438 |
Actin | 0.0094 | 0.0023 | 0.0055 | 0.0235 | 0.0199 | 0.0073 | 0.0148 | 0.0006 | 0.0016 | 0.0229 | 0.0032 | 0.0014 | 0.0193 | 0.0043 | 0.0104 | 0.0323 | 0.0298 | 0.0055 |
Bud | 0.001 | 0.0006 | 0.0014 | 0.0015 | 0.0014 | 0.001 | 0.0025 | 0.0033 | 0.0014 | 0.0029 | 0.0006 | 0.001 | 0.0098 | 0.0119 | 0.0145 | 0.0117 | 0.0072 | 0.005 |
Bud Neck | 0.0036 | 0.0006 | 0.001 | 0.0014 | 0.0015 | 0.0035 | 0.0018 | 0.0004 | 0.0007 | 0.0014 | 0.0006 | 0.0011 | 0.0011 | 0.0007 | 0.0011 | 0.0012 | 0.001 | 0.002 |
Bud Periphery | 0.0012 | 0.0004 | 0.0013 | 0.0012 | 0.0018 | 0.001 | 0.0022 | 0.0008 | 0.0006 | 0.0036 | 0.0004 | 0.001 | 0.0057 | 0.0038 | 0.006 | 0.0069 | 0.0028 | 0.0018 |
Bud Site | 0.0042 | 0.0016 | 0.0043 | 0.0021 | 0.0139 | 0.001 | 0.0062 | 0.0068 | 0.0011 | 0.0078 | 0.0006 | 0.0002 | 0.0093 | 0.0415 | 0.0158 | 0.0082 | 0.0014 | 0.0033 |
Cell Periphery | 0.002 | 0.0012 | 0.0013 | 0.0006 | 0.0005 | 0.0005 | 0.0012 | 0.0003 | 0.0004 | 0.0013 | 0.0001 | 0.0003 | 0.0015 | 0.001 | 0.0009 | 0.0007 | 0.0004 | 0.0004 |
Cytoplasm | 0.6016 | 0.918 | 0.7614 | 0.6415 | 0.549 | 0.7196 | 0.7055 | 0.9028 | 0.863 | 0.6056 | 0.6994 | 0.7974 | 0.7169 | 0.8475 | 0.8207 | 0.7941 | 0.6896 | 0.8001 |
Cytoplasmic Foci | 0.023 | 0.0096 | 0.0215 | 0.0255 | 0.0341 | 0.033 | 0.0183 | 0.0135 | 0.0198 | 0.0238 | 0.0357 | 0.0218 | 0.0305 | 0.0299 | 0.0221 | 0.0359 | 0.0362 | 0.0305 |
Eisosomes | 0.0003 | 0 | 0.0005 | 0.0002 | 0.0003 | 0 | 0.0004 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0.0002 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.1374 | 0.0226 | 0.0343 | 0.0416 | 0.0606 | 0.078 | 0.0771 | 0.0155 | 0.0248 | 0.0271 | 0.0119 | 0.0444 | 0.0352 | 0.0108 | 0.0163 | 0.0061 | 0.007 | 0.0346 |
Endosome | 0.1034 | 0.0242 | 0.078 | 0.0848 | 0.1462 | 0.0847 | 0.0821 | 0.0339 | 0.0508 | 0.1564 | 0.1682 | 0.0825 | 0.0714 | 0.0247 | 0.0468 | 0.0475 | 0.1492 | 0.0752 |
Golgi | 0.0188 | 0.0076 | 0.0116 | 0.0389 | 0.0277 | 0.0185 | 0.0173 | 0.006 | 0.0085 | 0.0256 | 0.0205 | 0.0189 | 0.0105 | 0.0031 | 0.0069 | 0.0097 | 0.0116 | 0.01 |
Lipid Particles | 0.0088 | 0.0002 | 0.0071 | 0.0078 | 0.03 | 0.0006 | 0.004 | 0.0001 | 0.0003 | 0.0048 | 0.004 | 0.0001 | 0.0046 | 0.0009 | 0.0007 | 0.0147 | 0.0024 | 0.0008 |
Mitochondria | 0.0172 | 0.0024 | 0.0126 | 0.0414 | 0.0324 | 0.0269 | 0.0274 | 0.0026 | 0.0041 | 0.0542 | 0.0133 | 0.0177 | 0.0188 | 0.002 | 0.005 | 0.0016 | 0.0021 | 0.0086 |
None | 0.0036 | 0.0005 | 0.0026 | 0.0006 | 0.0007 | 0.0011 | 0.0019 | 0.0005 | 0.0008 | 0.0015 | 0.002 | 0.0007 | 0.0109 | 0.0035 | 0.003 | 0.0064 | 0.0066 | 0.0022 |
Nuclear Periphery | 0.0116 | 0.0011 | 0.004 | 0.0044 | 0.007 | 0.0035 | 0.0069 | 0.0008 | 0.0013 | 0.0032 | 0.0152 | 0.001 | 0.0076 | 0.001 | 0.0022 | 0.0017 | 0.003 | 0.0041 |
Nucleolus | 0.001 | 0 | 0.0008 | 0.0001 | 0.0004 | 0.0009 | 0.0003 | 0 | 0 | 0.0003 | 0.0003 | 0 | 0.0038 | 0.0005 | 0.0028 | 0.0002 | 0.0003 | 0.0003 |
Nucleus | 0.0059 | 0.0009 | 0.0075 | 0.0015 | 0.0026 | 0.0021 | 0.0028 | 0.0008 | 0.0009 | 0.0019 | 0.0026 | 0.0007 | 0.0079 | 0.0031 | 0.0041 | 0.0017 | 0.0033 | 0.0029 |
Peroxisomes | 0.0018 | 0.0001 | 0.0037 | 0.0286 | 0.0384 | 0.0002 | 0.0014 | 0.0001 | 0.0002 | 0.0064 | 0.0009 | 0.0001 | 0.0013 | 0.0005 | 0.0005 | 0.0092 | 0.0029 | 0.0004 |
Punctate Nuclear | 0.0056 | 0.0001 | 0.0005 | 0.0003 | 0.0011 | 0.0006 | 0.0007 | 0.0001 | 0.0001 | 0.0016 | 0.0007 | 0 | 0.0038 | 0.0009 | 0.0008 | 0.0027 | 0.0026 | 0.0028 |
Vacuole | 0.0319 | 0.0047 | 0.0318 | 0.0417 | 0.019 | 0.0114 | 0.0195 | 0.0091 | 0.016 | 0.0331 | 0.0124 | 0.006 | 0.0253 | 0.007 | 0.0164 | 0.0056 | 0.0332 | 0.0069 |
Vacuole Periphery | 0.0066 | 0.0012 | 0.0072 | 0.0109 | 0.0112 | 0.0045 | 0.0059 | 0.0019 | 0.0037 | 0.0145 | 0.0073 | 0.0037 | 0.0045 | 0.0014 | 0.003 | 0.0017 | 0.0073 | 0.0026 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 40.4864 | 33.8515 | 25.6538 | 42.8157 | 43.8742 | 30.5996 | 49.1562 | 51.9775 | 38.1641 | 50.9308 |
Translational Efficiency | 1.3224 | 1.6973 | 1.6755 | 0.8687 | 1.0396 | 1.4217 | 1.1567 | 0.8779 | 1.0431 | 0.9098 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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