Standard name | |
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Human Ortholog | |
Description | Regulatory subunit of the vacuolar transporter chaperone (VTC) complex; involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; VTC2 has a paralog, VTC3, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 22 | 14 | 36 | 38 | 25 | 14 | 8 | 2 | 5 | 1 | 0 | 2 | 3 | 3 | 1 | 5 | 2 | 5 | 18 | 17 | 36 | 9 | 20 | 9 |
Bud | 7 | 2 | 8 | 11 | 16 | 22 | 25 | 20 | 25 | 28 | 12 | 19 | 1 | 1 | 0 | 4 | 1 | 2 | 4 | 4 | 4 | 2 | 3 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 6 | 4 | 0 | 11 | 9 | 10 | 2 | 6 | 8 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 33 | 36 | 52 | 47 | 27 | 21 | 12 | 7 | 4 | 2 | 6 | 7 | 28 | 27 | 12 | 9 | 13 | 15 | 4 | 9 | 22 | 8 | 15 | 14 |
Cytoplasm | 234 | 153 | 294 | 118 | 208 | 428 | 518 | 725 | 409 | 453 | 334 | 424 | 94 | 251 | 333 | 779 | 509 | 398 | 38 | 34 | 28 | 26 | 52 | 85 |
Endoplasmic Reticulum | 83 | 76 | 116 | 84 | 41 | 31 | 14 | 3 | 3 | 0 | 3 | 5 | 31 | 27 | 3 | 31 | 32 | 17 | 293 | 191 | 358 | 107 | 111 | 126 |
Endosome | 5 | 0 | 5 | 1 | 7 | 3 | 3 | 7 | 2 | 3 | 2 | 1 | 1 | 0 | 0 | 8 | 4 | 1 | 0 | 5 | 1 | 3 | 1 | 2 |
Golgi | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 7 | 4 | 3 | 1 | 1 | 1 | 1 |
Mitochondria | 3 | 1 | 2 | 2 | 2 | 21 | 30 | 54 | 48 | 79 | 66 | 79 | 0 | 0 | 0 | 26 | 7 | 5 | 5 | 4 | 17 | 16 | 13 | 16 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 4 | 0 | 1 | 3 | 0 | 0 | 1 | 0 | 7 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 0 | 4 | 1 | 0 | 1 | 2 | 3 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 26 | 0 | 7 | 4 | 9 | 9 | 16 | 11 | 6 | 13 | 4 | 12 | 0 | 1 | 1 | 43 | 19 | 19 | 19 | 12 | 23 | 9 | 19 | 41 |
Unique Cell Count | 363 | 244 | 451 | 234 | 278 | 485 | 567 | 779 | 441 | 504 | 373 | 481 | 137 | 287 | 337 | 838 | 536 | 436 | 404 | 296 | 512 | 191 | 250 | 314 |
Labelled Cell Count | 416 | 283 | 524 | 309 | 337 | 552 | 630 | 842 | 504 | 583 | 431 | 553 | 158 | 311 | 350 | 921 | 595 | 472 | 404 | 296 | 512 | 191 | 250 | 314 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.3 | 8.6 | 7.0 | 8.4 | 6.9 | 5.1 | 4.5 | 4.4 | 4.0 | 3.7 | 3.8 | 3.8 | 8.3 | 6.7 | 6.1 | 5.7 | 6.2 | 7.2 | 8.3 | 7.9 | 7.9 |
Std Deviation (1e-4) | 2.7 | 2.4 | 1.9 | 2.1 | 1.9 | 1.0 | 1.1 | 1.1 | 0.7 | 0.9 | 0.9 | 0.6 | 1.8 | 1.2 | 1.0 | 1.4 | 1.5 | 2.1 | 2.2 | 1.9 | 2.0 |
Intensity Change (Log2) | – | – | – | 0.26 | -0.03 | -0.46 | -0.66 | -0.66 | -0.82 | -0.92 | -0.89 | -0.88 | 0.24 | -0.08 | -0.21 | -0.29 | -0.17 | 0.04 | 0.24 | 0.16 | 0.16 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 3.3 | 0.5 | -3.5 | 0 | 0 | 0 | 0 | 0 | 0 | -2.4 | -4.1 | -5.0 | -7.2 | -6.2 | -4.8 |
Bud | 2.2 | 2.9 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 3.0 | -0.8 | -4.1 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | -0.9 | -4.1 | -8.4 | -5.7 | -4.6 |
Cytoplasm | -3.7 | 2.7 | 8.4 | 10.3 | 12.5 | 10.1 | 9.2 | 8.2 | 8.3 | 0.7 | 6.7 | 11.6 | 12.7 | 12.0 | 9.4 |
Endoplasmic Reticulum | 2.8 | -3.5 | -8.1 | 0 | 0 | 0 | 0 | 0 | 0 | -0.7 | -5.5 | -9.6 | -11.9 | -8.7 | -9.1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 1.5 | 0.4 | 1.4 | -0.2 | -0.2 | 1.1 | 0 | 1.0 | 0 | 0 | 0 | 3.2 | 1.9 | 2.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.0678 | 4.4567 | 3.9237 | 3.7601 | 3.9163 | 4.1537 | 6.0177 | 7.0347 | 7.0836 | 6.2175 | 5.0569 | 7.4091 | 7.4384 | 7.3791 | 7.3332 | 7.3452 | 6.9638 | 7.3735 |
Actin | 0.0201 | 0.0089 | 0.0092 | 0.02 | 0.0181 | 0.0042 | 0.0755 | 0.0057 | 0.0105 | 0.0498 | 0.0027 | 0.0105 | 0.001 | 0.0056 | 0.0092 | 0.0045 | 0.0086 | 0.0042 |
Bud | 0.0011 | 0.0049 | 0.0006 | 0.0002 | 0.0023 | 0.0043 | 0.0009 | 0.0008 | 0.0005 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.001 | 0.0008 | 0.0006 | 0.0004 |
Bud Neck | 0.0118 | 0.0018 | 0.0051 | 0.0096 | 0.0116 | 0.0199 | 0.0069 | 0.0022 | 0.0057 | 0.0043 | 0.0028 | 0.0111 | 0.0008 | 0.0021 | 0.0063 | 0.0041 | 0.0042 | 0.0085 |
Bud Periphery | 0.006 | 0.0025 | 0.0019 | 0.0008 | 0.0041 | 0.0019 | 0.002 | 0.0032 | 0.0012 | 0.0012 | 0.0008 | 0.0008 | 0.0008 | 0.0021 | 0.0036 | 0.0043 | 0.0045 | 0.0039 |
Bud Site | 0.0092 | 0.0408 | 0.0102 | 0.0014 | 0.0045 | 0.0006 | 0.008 | 0.0209 | 0.0164 | 0.0021 | 0.001 | 0.0004 | 0.001 | 0.0387 | 0.0113 | 0.0031 | 0.0016 | 0.0007 |
Cell Periphery | 0.0187 | 0.0149 | 0.0219 | 0.0289 | 0.02 | 0.0085 | 0.0235 | 0.0547 | 0.0228 | 0.027 | 0.0223 | 0.0145 | 0.0378 | 0.0328 | 0.0418 | 0.0506 | 0.0285 | 0.021 |
Cytoplasm | 0.4138 | 0.5838 | 0.5071 | 0.428 | 0.5331 | 0.5507 | 0.2651 | 0.5258 | 0.5558 | 0.4698 | 0.3126 | 0.3584 | 0.3851 | 0.3809 | 0.4691 | 0.4784 | 0.4936 | 0.5326 |
Cytoplasmic Foci | 0.0256 | 0.016 | 0.0155 | 0.0128 | 0.0131 | 0.0139 | 0.0719 | 0.0069 | 0.0099 | 0.0155 | 0.0971 | 0.018 | 0.0045 | 0.0067 | 0.0073 | 0.0066 | 0.0089 | 0.0073 |
Eisosomes | 0.0013 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0017 | 0.0006 | 0.0004 | 0.0002 | 0.0007 | 0.0002 | 0.0002 | 0.0004 | 0.0005 | 0.0003 | 0.0008 | 0.0005 |
Endoplasmic Reticulum | 0.3425 | 0.2355 | 0.3076 | 0.3629 | 0.2931 | 0.2992 | 0.1718 | 0.2969 | 0.2789 | 0.2857 | 0.4593 | 0.4674 | 0.5189 | 0.4593 | 0.388 | 0.3814 | 0.3946 | 0.3705 |
Endosome | 0.0357 | 0.0173 | 0.0243 | 0.0307 | 0.0254 | 0.0219 | 0.0866 | 0.0039 | 0.0191 | 0.0468 | 0.0244 | 0.0099 | 0.0053 | 0.0084 | 0.0083 | 0.0084 | 0.0059 | 0.006 |
Golgi | 0.0133 | 0.007 | 0.0117 | 0.0118 | 0.0135 | 0.0032 | 0.0543 | 0.0023 | 0.008 | 0.039 | 0.0058 | 0.007 | 0.0019 | 0.004 | 0.0062 | 0.0071 | 0.0044 | 0.0035 |
Lipid Particles | 0.0326 | 0.0222 | 0.0193 | 0.0334 | 0.0166 | 0.0092 | 0.0584 | 0.0233 | 0.0178 | 0.0319 | 0.0236 | 0.0368 | 0.0203 | 0.027 | 0.0186 | 0.0139 | 0.0197 | 0.0141 |
Mitochondria | 0.0065 | 0.0006 | 0.0029 | 0.0118 | 0.0134 | 0.0013 | 0.0129 | 0.0001 | 0.001 | 0.0016 | 0.0003 | 0.0114 | 0.0001 | 0.0004 | 0.0004 | 0.0003 | 0.0004 | 0.0004 |
None | 0.0266 | 0.0263 | 0.0467 | 0.0257 | 0.0133 | 0.041 | 0.0529 | 0.0191 | 0.0361 | 0.0101 | 0.0081 | 0.0414 | 0.0082 | 0.0137 | 0.0157 | 0.0084 | 0.0097 | 0.0171 |
Nuclear Periphery | 0.0102 | 0.0042 | 0.004 | 0.0061 | 0.0044 | 0.0092 | 0.0232 | 0.0066 | 0.0052 | 0.0026 | 0.0036 | 0.0026 | 0.0037 | 0.0037 | 0.0031 | 0.0044 | 0.0028 | 0.0018 |
Nucleolus | 0.0012 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0039 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0001 |
Nucleus | 0.0073 | 0.0022 | 0.0013 | 0.0014 | 0.0014 | 0.0021 | 0.0062 | 0.0036 | 0.0012 | 0.0009 | 0.001 | 0.0007 | 0.0008 | 0.0016 | 0.0011 | 0.0017 | 0.0035 | 0.0011 |
Peroxisomes | 0.0028 | 0.0003 | 0.0007 | 0.0019 | 0.0016 | 0.0003 | 0.0397 | 0.0001 | 0.0002 | 0.0006 | 0.0054 | 0.001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Punctate Nuclear | 0.0021 | 0.001 | 0.0007 | 0.0017 | 0.0005 | 0.0009 | 0.0257 | 0.0007 | 0.001 | 0.0007 | 0.0216 | 0.0005 | 0.0002 | 0.0007 | 0.0003 | 0.0005 | 0.0008 | 0.0005 |
Vacuole | 0.0096 | 0.009 | 0.0082 | 0.0097 | 0.0087 | 0.0066 | 0.0059 | 0.0218 | 0.0074 | 0.0089 | 0.0065 | 0.0071 | 0.0088 | 0.0108 | 0.0076 | 0.0208 | 0.0063 | 0.0054 |
Vacuole Periphery | 0.0016 | 0.0004 | 0.0006 | 0.0008 | 0.0009 | 0.0008 | 0.003 | 0.0003 | 0.0006 | 0.0007 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 103.153 | 117.794 | 123.019 | 133.9088 | 120.2243 | 90.4583 | 123.0113 | 123.5554 | 146.1555 | 159.6648 |
Translational Efficiency | 1.23 | 1.3089 | 1.2432 | 1.2804 | 1.1959 | 1.515 | 1.2073 | 1.2448 | 1.0715 | 1.1177 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
705 | 1235 | 156 | 1742 | 2266 | 2175 | 209 | 1439 | 2971 | 3410 | 365 | 3181 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 910.16 | 969.20 | 1023.92 | 1059.29 | 1075.78 | 928.60 | 1140.94 | 1043.18 | 1036.48 | 943.30 | 1090.93 | 1052.00 |
Standard Deviation | 144.30 | 176.97 | 104.21 | 150.33 | 172.09 | 147.76 | 119.09 | 154.76 | 180.26 | 160.15 | 126.94 | 152.56 |
Intensity Change Log 2 | — | 0.090674 | 0.169911 | 0.218906 | — | -0.212254 | 0.084840 | -0.044395 | — | -0.065494 | 0.124452 | 0.082263 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.014437 | 0.008008 | 0.002204 | 0.032191 | 0.002219 | 0.005321 | 0.021761 | 0.032919 | 0.005119 | 0.006294 | 0.013402 | 0.032521 |
Bud Neck | 0.015960 | 0.019315 | 0.007094 | 0.028663 | 0.004817 | 0.022660 | 0.012416 | 0.026442 | 0.007461 | 0.021449 | 0.010141 | 0.027658 |
Bud Site | 0.066421 | 0.052866 | 0.020045 | 0.103864* | 0.012174 | 0.039773 | 0.051591 | 0.091998 | 0.025047 | 0.044515 | 0.038108 | 0.098496 |
Cell Periphery | 0.010856 | 0.029682 | 0.002543 | 0.022213 | 0.057033 | 0.016007 | 0.032260 | 0.035529 | 0.046075 | 0.020960 | 0.019559 | 0.028237 |
Cytoplasm | 0.256335* | 0.290489* | 0.261124* | 0.284864* | 0.216767* | 0.363852* | 0.123030* | 0.271124* | 0.226156* | 0.337282* | 0.182051* | 0.278648* |
Cytoplasmic Foci | 0.071835 | 0.065352 | 0.122925 | 0.042426 | 0.015673 | 0.081997 | 0.023995 | 0.034890 | 0.029000 | 0.075969 | 0.066277 | 0.039017 |
Eisosomes | 0.003483 | 0.005029 | 0.000651 | 0.005655 | 0.003506 | 0.006345 | 0.006140 | 0.008671 | 0.003500 | 0.005869 | 0.003794 | 0.007019 |
Endoplasmic Reticulum | 0.184238* | 0.367729* | 0.087518 | 0.182759* | 0.573106* | 0.253092* | 0.510953* | 0.268036* | 0.480830* | 0.294610* | 0.329978* | 0.221336* |
Endosome | 0.021505 | 0.017882 | 0.027428 | 0.012364 | 0.006780 | 0.021623 | 0.008548 | 0.011124 | 0.010274 | 0.020268 | 0.016617 | 0.011803 |
Golgi | 0.011041 | 0.027637 | 0.001257 | 0.006064 | 0.009071 | 0.036492 | 0.005702 | 0.008098 | 0.009539 | 0.033285 | 0.003802 | 0.006984 |
Lipid Particles | 0.021213 | 0.034698 | 0.028381 | 0.037633 | 0.032228 | 0.042653 | 0.032574 | 0.048292 | 0.029614 | 0.039772 | 0.030782 | 0.042455 |
Mitochondria | 0.006611 | 0.007310 | 0.000142 | 0.004407 | 0.005829 | 0.017663 | 0.000142 | 0.004376 | 0.006015 | 0.013914 | 0.000142 | 0.004393 |
Mitotic Spindle | 0.006750 | 0.001277 | 0.000313 | 0.011141 | 0.000102 | 0.000781 | 0.000200 | 0.003283 | 0.001680 | 0.000961 | 0.000248 | 0.007586 |
None | 0.000234 | 0.001324 | 0.000189 | 0.000863 | 0.002427 | 0.003897 | 0.000062 | 0.001151 | 0.001907 | 0.002965 | 0.000116 | 0.000993 |
Nuclear Periphery | 0.000584 | 0.000173 | 0.001904 | 0.000708 | 0.000341 | 0.000359 | 0.000495 | 0.000314 | 0.000399 | 0.000291 | 0.001097 | 0.000530 |
Nuclear Periphery Foci | 0.005205 | 0.001561 | 0.050760 | 0.008047 | 0.000757 | 0.001148 | 0.020421 | 0.006549 | 0.001813 | 0.001298 | 0.033388 | 0.007369 |
Nucleolus | 0.000282 | 0.000228 | 0.000239 | 0.000256 | 0.000099 | 0.000619 | 0.000037 | 0.000193 | 0.000143 | 0.000478 | 0.000123 | 0.000228 |
Nucleus | 0.024458 | 0.009253 | 0.092318 | 0.047904 | 0.002535 | 0.019586 | 0.019853 | 0.027324 | 0.007737 | 0.015844 | 0.050824 | 0.038594 |
Peroxisomes | 0.005929 | 0.013447 | 0.001430 | 0.005146 | 0.035109 | 0.014964 | 0.001419 | 0.008348 | 0.028185 | 0.014415 | 0.001424 | 0.006594 |
Vacuole | 0.267395* | 0.042860 | 0.291071* | 0.161317 | 0.017522 | 0.046353 | 0.128263 | 0.109609 | 0.076815 | 0.045088 | 0.197847 | 0.137926 |
Vacuole Periphery | 0.005230 | 0.003879 | 0.000463 | 0.001515 | 0.001904 | 0.004813 | 0.000140 | 0.001729 | 0.002694 | 0.004474 | 0.000278 | 0.001612 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.67 | 6.27 | -6.23 | -10.06 | -14.09 | -4.86 | -5.44 | -14.87 | -12.86 | -2.47 | -2.05 | -3.92 | -17.60 | -16.48 | -6.88 |
Bud Neck | -2.13 | 4.20 | -5.67 | -3.26 | -9.70 | -13.16 | -3.71 | -13.19 | -1.51 | -5.22 | -12.86 | -2.03 | -16.04 | -3.15 | -9.52 |
Bud Site | 1.61 | 6.57 | -4.90 | -8.18 | -13.61 | -11.25 | -4.89 | -17.30 | -10.25 | -3.75 | -8.27 | -2.73 | -20.39 | -13.86 | -9.06 |
Cell Periphery | -7.34 | 6.20 | -5.04 | 3.68 | -11.61 | 16.28 | 4.36 | 7.62 | -7.53 | -0.36 | 11.77 | 7.33 | 9.21 | -2.90 | -1.97 |
Cytoplasm | -3.31 | 0.09 | -2.90 | 0.78 | -1.83 | -20.54 | 8.25 | -7.27 | 11.36 | -12.61 | -18.58 | 4.42 | -9.04 | 9.90 | -9.48 |
Cytoplasmic Foci | 1.02 | -5.70 | 6.78 | 6.71 | 9.73 | -24.53 | -2.74 | -10.47 | 14.94 | -3.24 | -20.76 | -7.86 | -6.09 | 15.69 | 5.51 |
Eisosomes | -2.75 | 5.22 | -2.99 | 0.03 | -12.58 | -7.06 | -2.24 | -8.27 | -3.07 | -1.85 | -7.20 | -0.40 | -8.23 | -1.51 | -3.90 |
Endoplasmic Reticulum | -13.17 | 5.64 | 0.59 | 17.05 | -6.31 | 33.87* | 2.97 | 29.58 | -0.05 | 9.45 | 20.98 | 8.10 | 32.48 | 11.89 | 6.13 |
Endosome | 1.31 | -2.43 | 5.35 | 5.00 | 6.35 | -16.32 | -2.35 | -4.75 | 9.58 | -1.99 | -13.09 | -5.21 | -1.81 | 10.77 | 4.07 |
Golgi | -7.53 | 5.13 | 2.65 | 13.57 | -7.40 | -17.74 | 3.90 | 1.86 | 18.66 | -2.31 | -19.46 | 7.73 | 4.25 | 23.45 | -4.63 |
Lipid Particles | -5.97 | -2.68 | -6.10 | -0.09 | -2.62 | -4.92 | -0.10 | -4.90 | -1.31 | -3.18 | -6.23 | -0.42 | -5.62 | -0.19 | -3.59 |
Mitochondria | -0.65 | 3.30 | 1.10 | 3.12 | -5.27 | -11.74 | 14.29 | 1.68 | 10.36 | -4.35 | -9.51 | 11.03 | 2.40 | 11.27 | -6.78 |
Mitotic Spindle | 2.71 | 3.54 | -1.60 | -4.78 | -5.83 | -2.70 | -1.90 | -3.54 | -2.64 | -3.35 | 1.16 | 3.17 | -5.02 | -5.71 | -6.68 |
None | -2.88 | 0.32 | -2.20 | 1.20 | -2.46 | -2.19 | 6.28 | 2.00 | 3.59 | -1.95 | -2.23 | 6.19 | 2.41 | 4.30 | -2.94 |
Nuclear Periphery | 4.86 | -5.83 | -1.42 | -4.45 | 4.36 | -0.44 | -3.40 | 0.38 | 0.62 | 3.20 | 3.09 | -6.86 | -2.32 | -3.55 | 4.17 |
Nuclear Periphery Foci | 8.33 | -9.64 | -4.58 | -12.56 | 8.97 | -2.59 | -10.22 | -10.84 | -9.82 | 7.00 | 3.25 | -13.20 | -14.65 | -16.01 | 10.71 |
Nucleolus | 0.33 | 0.24 | 0.20 | -0.50 | -0.19 | -4.00 | 7.74 | -3.38 | 3.24 | -5.56 | -3.70 | 0.41 | -2.06 | 3.00 | -4.67 |
Nucleus | 7.50 | -7.13 | -9.19 | -19.29 | 4.47 | -20.69 | -7.63 | -18.09 | -5.77 | -2.70 | -10.42 | -9.44 | -23.57 | -17.55 | 2.38 |
Peroxisomes | -6.05 | 4.18 | 0.75 | 9.26 | -9.21 | 11.65 | 21.57 | 16.57 | 7.56 | -11.57 | 10.07 | 21.64 | 17.33 | 12.56 | -14.53 |
Vacuole | 22.28 | -0.71 | 7.71 | -25.26 | 6.28 | -12.29 | -9.91 | -21.46 | -15.13 | 0.55 | 9.96 | -11.18 | -16.16 | -29.25 | 3.68 |
Vacuole Periphery | 0.73 | 3.72 | 2.84 | 4.15 | -2.46 | -7.85 | 9.00 | 0.73 | 7.06 | -4.60 | -4.52 | 7.29 | 2.83 | 8.29 | -4.89 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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