Standard name | |
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Human Ortholog | |
Description | Regulatory subunit of the vacuolar transporter chaperone (VTC) complex; involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; VTC2 has a paralog, VTC3, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 22 | 14 | 36 | 38 | 25 | 14 | 8 | 2 | 5 | 1 | 0 | 2 | 3 | 3 | 1 | 5 | 2 | 5 | 18 | 17 | 36 | 9 | 20 | 9 |
Bud | 7 | 2 | 8 | 11 | 16 | 22 | 25 | 20 | 25 | 28 | 12 | 19 | 1 | 1 | 0 | 4 | 1 | 2 | 4 | 4 | 4 | 2 | 3 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 6 | 4 | 0 | 11 | 9 | 10 | 2 | 6 | 8 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 33 | 36 | 52 | 47 | 27 | 21 | 12 | 7 | 4 | 2 | 6 | 7 | 28 | 27 | 12 | 9 | 13 | 15 | 4 | 9 | 22 | 8 | 15 | 14 |
Cytoplasm | 234 | 153 | 294 | 118 | 208 | 428 | 518 | 725 | 409 | 453 | 334 | 424 | 94 | 251 | 333 | 779 | 509 | 398 | 38 | 34 | 28 | 26 | 52 | 85 |
Endoplasmic Reticulum | 83 | 76 | 116 | 84 | 41 | 31 | 14 | 3 | 3 | 0 | 3 | 5 | 31 | 27 | 3 | 31 | 32 | 17 | 293 | 191 | 358 | 107 | 111 | 126 |
Endosome | 5 | 0 | 5 | 1 | 7 | 3 | 3 | 7 | 2 | 3 | 2 | 1 | 1 | 0 | 0 | 8 | 4 | 1 | 0 | 5 | 1 | 3 | 1 | 2 |
Golgi | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 7 | 4 | 3 | 1 | 1 | 1 | 1 |
Mitochondria | 3 | 1 | 2 | 2 | 2 | 21 | 30 | 54 | 48 | 79 | 66 | 79 | 0 | 0 | 0 | 26 | 7 | 5 | 5 | 4 | 17 | 16 | 13 | 16 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 4 | 0 | 1 | 3 | 0 | 0 | 1 | 0 | 7 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2 | 0 | 4 | 1 | 0 | 1 | 2 | 3 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 26 | 0 | 7 | 4 | 9 | 9 | 16 | 11 | 6 | 13 | 4 | 12 | 0 | 1 | 1 | 43 | 19 | 19 | 19 | 12 | 23 | 9 | 19 | 41 |
Unique Cell Count | 363 | 244 | 451 | 234 | 278 | 485 | 567 | 779 | 441 | 504 | 373 | 481 | 137 | 287 | 337 | 838 | 536 | 436 | 404 | 296 | 512 | 191 | 250 | 314 |
Labelled Cell Count | 416 | 283 | 524 | 309 | 337 | 552 | 630 | 842 | 504 | 583 | 431 | 553 | 158 | 311 | 350 | 921 | 595 | 472 | 404 | 296 | 512 | 191 | 250 | 314 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.3 | 8.6 | 7.0 | 8.4 | 6.9 | 5.1 | 4.5 | 4.4 | 4.0 | 3.7 | 3.8 | 3.8 | 8.3 | 6.7 | 6.1 | 5.7 | 6.2 | 7.2 | 8.3 | 7.9 | 7.9 |
Std Deviation (1e-4) | 2.7 | 2.4 | 1.9 | 2.1 | 1.9 | 1.0 | 1.1 | 1.1 | 0.7 | 0.9 | 0.9 | 0.6 | 1.8 | 1.2 | 1.0 | 1.4 | 1.5 | 2.1 | 2.2 | 1.9 | 2.0 |
Intensity Change (Log2) | – | – | – | 0.26 | -0.03 | -0.46 | -0.66 | -0.66 | -0.82 | -0.92 | -0.89 | -0.88 | 0.24 | -0.08 | -0.21 | -0.29 | -0.17 | 0.04 | 0.24 | 0.16 | 0.16 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 3.3 | 0.5 | -3.5 | 0 | 0 | 0 | 0 | 0 | 0 | -2.4 | -4.1 | -5.0 | -7.2 | -6.2 | -4.8 |
Bud | 2.2 | 2.9 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 3.0 | -0.8 | -4.1 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | -0.9 | -4.1 | -8.4 | -5.7 | -4.6 |
Cytoplasm | -3.7 | 2.7 | 8.4 | 10.3 | 12.5 | 10.1 | 9.2 | 8.2 | 8.3 | 0.7 | 6.7 | 11.6 | 12.7 | 12.0 | 9.4 |
Endoplasmic Reticulum | 2.8 | -3.5 | -8.1 | 0 | 0 | 0 | 0 | 0 | 0 | -0.7 | -5.5 | -9.6 | -11.9 | -8.7 | -9.1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 1.5 | 0.4 | 1.4 | -0.2 | -0.2 | 1.1 | 0 | 1.0 | 0 | 0 | 0 | 3.2 | 1.9 | 2.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 4.0678 | 4.4567 | 3.9237 | 3.7601 | 3.9163 | 4.1537 | 6.0177 | 7.0347 | 7.0836 | 6.2175 | 5.0569 | 7.4091 | 7.4384 | 7.3791 | 7.3332 | 7.3452 | 6.9638 | 7.3735 |
Actin | 0.0201 | 0.0089 | 0.0092 | 0.02 | 0.0181 | 0.0042 | 0.0755 | 0.0057 | 0.0105 | 0.0498 | 0.0027 | 0.0105 | 0.001 | 0.0056 | 0.0092 | 0.0045 | 0.0086 | 0.0042 |
Bud | 0.0011 | 0.0049 | 0.0006 | 0.0002 | 0.0023 | 0.0043 | 0.0009 | 0.0008 | 0.0005 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.001 | 0.0008 | 0.0006 | 0.0004 |
Bud Neck | 0.0118 | 0.0018 | 0.0051 | 0.0096 | 0.0116 | 0.0199 | 0.0069 | 0.0022 | 0.0057 | 0.0043 | 0.0028 | 0.0111 | 0.0008 | 0.0021 | 0.0063 | 0.0041 | 0.0042 | 0.0085 |
Bud Periphery | 0.006 | 0.0025 | 0.0019 | 0.0008 | 0.0041 | 0.0019 | 0.002 | 0.0032 | 0.0012 | 0.0012 | 0.0008 | 0.0008 | 0.0008 | 0.0021 | 0.0036 | 0.0043 | 0.0045 | 0.0039 |
Bud Site | 0.0092 | 0.0408 | 0.0102 | 0.0014 | 0.0045 | 0.0006 | 0.008 | 0.0209 | 0.0164 | 0.0021 | 0.001 | 0.0004 | 0.001 | 0.0387 | 0.0113 | 0.0031 | 0.0016 | 0.0007 |
Cell Periphery | 0.0187 | 0.0149 | 0.0219 | 0.0289 | 0.02 | 0.0085 | 0.0235 | 0.0547 | 0.0228 | 0.027 | 0.0223 | 0.0145 | 0.0378 | 0.0328 | 0.0418 | 0.0506 | 0.0285 | 0.021 |
Cytoplasm | 0.4138 | 0.5838 | 0.5071 | 0.428 | 0.5331 | 0.5507 | 0.2651 | 0.5258 | 0.5558 | 0.4698 | 0.3126 | 0.3584 | 0.3851 | 0.3809 | 0.4691 | 0.4784 | 0.4936 | 0.5326 |
Cytoplasmic Foci | 0.0256 | 0.016 | 0.0155 | 0.0128 | 0.0131 | 0.0139 | 0.0719 | 0.0069 | 0.0099 | 0.0155 | 0.0971 | 0.018 | 0.0045 | 0.0067 | 0.0073 | 0.0066 | 0.0089 | 0.0073 |
Eisosomes | 0.0013 | 0.0002 | 0.0004 | 0.0003 | 0.0002 | 0.0001 | 0.0017 | 0.0006 | 0.0004 | 0.0002 | 0.0007 | 0.0002 | 0.0002 | 0.0004 | 0.0005 | 0.0003 | 0.0008 | 0.0005 |
Endoplasmic Reticulum | 0.3425 | 0.2355 | 0.3076 | 0.3629 | 0.2931 | 0.2992 | 0.1718 | 0.2969 | 0.2789 | 0.2857 | 0.4593 | 0.4674 | 0.5189 | 0.4593 | 0.388 | 0.3814 | 0.3946 | 0.3705 |
Endosome | 0.0357 | 0.0173 | 0.0243 | 0.0307 | 0.0254 | 0.0219 | 0.0866 | 0.0039 | 0.0191 | 0.0468 | 0.0244 | 0.0099 | 0.0053 | 0.0084 | 0.0083 | 0.0084 | 0.0059 | 0.006 |
Golgi | 0.0133 | 0.007 | 0.0117 | 0.0118 | 0.0135 | 0.0032 | 0.0543 | 0.0023 | 0.008 | 0.039 | 0.0058 | 0.007 | 0.0019 | 0.004 | 0.0062 | 0.0071 | 0.0044 | 0.0035 |
Lipid Particles | 0.0326 | 0.0222 | 0.0193 | 0.0334 | 0.0166 | 0.0092 | 0.0584 | 0.0233 | 0.0178 | 0.0319 | 0.0236 | 0.0368 | 0.0203 | 0.027 | 0.0186 | 0.0139 | 0.0197 | 0.0141 |
Mitochondria | 0.0065 | 0.0006 | 0.0029 | 0.0118 | 0.0134 | 0.0013 | 0.0129 | 0.0001 | 0.001 | 0.0016 | 0.0003 | 0.0114 | 0.0001 | 0.0004 | 0.0004 | 0.0003 | 0.0004 | 0.0004 |
None | 0.0266 | 0.0263 | 0.0467 | 0.0257 | 0.0133 | 0.041 | 0.0529 | 0.0191 | 0.0361 | 0.0101 | 0.0081 | 0.0414 | 0.0082 | 0.0137 | 0.0157 | 0.0084 | 0.0097 | 0.0171 |
Nuclear Periphery | 0.0102 | 0.0042 | 0.004 | 0.0061 | 0.0044 | 0.0092 | 0.0232 | 0.0066 | 0.0052 | 0.0026 | 0.0036 | 0.0026 | 0.0037 | 0.0037 | 0.0031 | 0.0044 | 0.0028 | 0.0018 |
Nucleolus | 0.0012 | 0.0003 | 0.0002 | 0.0002 | 0.0002 | 0.0002 | 0.0039 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0001 |
Nucleus | 0.0073 | 0.0022 | 0.0013 | 0.0014 | 0.0014 | 0.0021 | 0.0062 | 0.0036 | 0.0012 | 0.0009 | 0.001 | 0.0007 | 0.0008 | 0.0016 | 0.0011 | 0.0017 | 0.0035 | 0.0011 |
Peroxisomes | 0.0028 | 0.0003 | 0.0007 | 0.0019 | 0.0016 | 0.0003 | 0.0397 | 0.0001 | 0.0002 | 0.0006 | 0.0054 | 0.001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 |
Punctate Nuclear | 0.0021 | 0.001 | 0.0007 | 0.0017 | 0.0005 | 0.0009 | 0.0257 | 0.0007 | 0.001 | 0.0007 | 0.0216 | 0.0005 | 0.0002 | 0.0007 | 0.0003 | 0.0005 | 0.0008 | 0.0005 |
Vacuole | 0.0096 | 0.009 | 0.0082 | 0.0097 | 0.0087 | 0.0066 | 0.0059 | 0.0218 | 0.0074 | 0.0089 | 0.0065 | 0.0071 | 0.0088 | 0.0108 | 0.0076 | 0.0208 | 0.0063 | 0.0054 |
Vacuole Periphery | 0.0016 | 0.0004 | 0.0006 | 0.0008 | 0.0009 | 0.0008 | 0.003 | 0.0003 | 0.0006 | 0.0007 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 103.153 | 117.794 | 123.019 | 133.9088 | 120.2243 | 90.4583 | 123.0113 | 123.5554 | 146.1555 | 159.6648 |
Translational Efficiency | 1.23 | 1.3089 | 1.2432 | 1.2804 | 1.1959 | 1.515 | 1.2073 | 1.2448 | 1.0715 | 1.1177 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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