Standard name
Human Ortholog
Description Regulatory subunit of the vacuolar transporter chaperone (VTC) complex; involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; VTC2 has a paralog, VTC3, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.06 0.08 0.16 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.07 0.05 0.08 0
Bud 0 0 0 0.05 0.06 0.05 0 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.09 0.15 0.12 0.2 0.1 0 0 0 0 0 0 0 0.2 0.09 0 0 0 0 0 0 0 0.05 0.06 0.05
Cytoplasm 0.64 0.63 0.65 0.5 0.75 0.88 0.91 0.93 0.93 0.9 0.9 0.88 0.69 0.87 0.99 0.93 0.95 0.91 0.09 0.12 0.06 0.14 0.21 0.27
Endoplasmic Reticulum 0.23 0.31 0.26 0.36 0.15 0.06 0 0 0 0 0 0 0.23 0.09 0 0 0.06 0 0.73 0.65 0.7 0.56 0.44 0.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 0.07 0.11 0.16 0.18 0.16 0 0 0 0 0 0 0 0 0 0.09 0.05 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0.05 0.05 0.08 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 22 14 36 38 25 14 8 2 5 1 0 2 3 3 1 5 2 5 18 17 36 9 20 9
Bud 7 2 8 11 16 22 25 20 25 28 12 19 1 1 0 4 1 2 4 4 4 2 3 2
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 6 4 0 11 9 10 2 6 8
Bud Site 0 0 0 0 0 2 0 1 0 1 0 1 0 0 0 0 0 0
Cell Periphery 33 36 52 47 27 21 12 7 4 2 6 7 28 27 12 9 13 15 4 9 22 8 15 14
Cytoplasm 234 153 294 118 208 428 518 725 409 453 334 424 94 251 333 779 509 398 38 34 28 26 52 85
Endoplasmic Reticulum 83 76 116 84 41 31 14 3 3 0 3 5 31 27 3 31 32 17 293 191 358 107 111 126
Endosome 5 0 5 1 7 3 3 7 2 3 2 1 1 0 0 8 4 1 0 5 1 3 1 2
Golgi 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1 7 4 3 1 1 1 1
Mitochondria 3 1 2 2 2 21 30 54 48 79 66 79 0 0 0 26 7 5 5 4 17 16 13 16
Nucleus 0 0 0 0 1 0 1 4 0 1 3 0 0 1 0 7 3 3 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 5 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 3 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0
SpindlePole 2 0 4 1 0 1 2 3 2 1 0 2 0 0 0 1 0 0 0 0 0 0 0 0
Vac/Vac Membrane 26 0 7 4 9 9 16 11 6 13 4 12 0 1 1 43 19 19 19 12 23 9 19 41
Unique Cell Count 363 244 451 234 278 485 567 779 441 504 373 481 137 287 337 838 536 436 404 296 512 191 250 314
Labelled Cell Count 416 283 524 309 337 552 630 842 504 583 431 553 158 311 350 921 595 472 404 296 512 191 250 314


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.3 8.6 7.0 8.4 6.9 5.1 4.5 4.4 4.0 3.7 3.8 3.8 8.3 6.7 6.1 5.7 6.2 7.2 8.3 7.9 7.9
Std Deviation (1e-4) 2.7 2.4 1.9 2.1 1.9 1.0 1.1 1.1 0.7 0.9 0.9 0.6 1.8 1.2 1.0 1.4 1.5 2.1 2.2 1.9 2.0
Intensity Change (Log2) 0.26 -0.03 -0.46 -0.66 -0.66 -0.82 -0.92 -0.89 -0.88 0.24 -0.08 -0.21 -0.29 -0.17 0.04 0.24 0.16 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 3.3 0.5 -3.5 0 0 0 0 0 0 -2.4 -4.1 -5.0 -7.2 -6.2 -4.8
Bud 2.2 2.9 2.4 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3.0 -0.8 -4.1 0 0 0 0 0 0 2.7 -0.9 -4.1 -8.4 -5.7 -4.6
Cytoplasm -3.7 2.7 8.4 10.3 12.5 10.1 9.2 8.2 8.3 0.7 6.7 11.6 12.7 12.0 9.4
Endoplasmic Reticulum 2.8 -3.5 -8.1 0 0 0 0 0 0 -0.7 -5.5 -9.6 -11.9 -8.7 -9.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.8 0 0 0 0 0 0 0 0 0 3.1 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.5 0.4 1.4 -0.2 -0.2 1.1 0 1.0 0 0 0 3.2 1.9 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0678 4.4567 3.9237 3.7601 3.9163 4.1537 6.0177 7.0347 7.0836 6.2175 5.0569 7.4091 7.4384 7.3791 7.3332 7.3452 6.9638 7.3735
Actin 0.0201 0.0089 0.0092 0.02 0.0181 0.0042 0.0755 0.0057 0.0105 0.0498 0.0027 0.0105 0.001 0.0056 0.0092 0.0045 0.0086 0.0042
Bud 0.0011 0.0049 0.0006 0.0002 0.0023 0.0043 0.0009 0.0008 0.0005 0.0005 0.0001 0.0002 0.0001 0.0007 0.001 0.0008 0.0006 0.0004
Bud Neck 0.0118 0.0018 0.0051 0.0096 0.0116 0.0199 0.0069 0.0022 0.0057 0.0043 0.0028 0.0111 0.0008 0.0021 0.0063 0.0041 0.0042 0.0085
Bud Periphery 0.006 0.0025 0.0019 0.0008 0.0041 0.0019 0.002 0.0032 0.0012 0.0012 0.0008 0.0008 0.0008 0.0021 0.0036 0.0043 0.0045 0.0039
Bud Site 0.0092 0.0408 0.0102 0.0014 0.0045 0.0006 0.008 0.0209 0.0164 0.0021 0.001 0.0004 0.001 0.0387 0.0113 0.0031 0.0016 0.0007
Cell Periphery 0.0187 0.0149 0.0219 0.0289 0.02 0.0085 0.0235 0.0547 0.0228 0.027 0.0223 0.0145 0.0378 0.0328 0.0418 0.0506 0.0285 0.021
Cytoplasm 0.4138 0.5838 0.5071 0.428 0.5331 0.5507 0.2651 0.5258 0.5558 0.4698 0.3126 0.3584 0.3851 0.3809 0.4691 0.4784 0.4936 0.5326
Cytoplasmic Foci 0.0256 0.016 0.0155 0.0128 0.0131 0.0139 0.0719 0.0069 0.0099 0.0155 0.0971 0.018 0.0045 0.0067 0.0073 0.0066 0.0089 0.0073
Eisosomes 0.0013 0.0002 0.0004 0.0003 0.0002 0.0001 0.0017 0.0006 0.0004 0.0002 0.0007 0.0002 0.0002 0.0004 0.0005 0.0003 0.0008 0.0005
Endoplasmic Reticulum 0.3425 0.2355 0.3076 0.3629 0.2931 0.2992 0.1718 0.2969 0.2789 0.2857 0.4593 0.4674 0.5189 0.4593 0.388 0.3814 0.3946 0.3705
Endosome 0.0357 0.0173 0.0243 0.0307 0.0254 0.0219 0.0866 0.0039 0.0191 0.0468 0.0244 0.0099 0.0053 0.0084 0.0083 0.0084 0.0059 0.006
Golgi 0.0133 0.007 0.0117 0.0118 0.0135 0.0032 0.0543 0.0023 0.008 0.039 0.0058 0.007 0.0019 0.004 0.0062 0.0071 0.0044 0.0035
Lipid Particles 0.0326 0.0222 0.0193 0.0334 0.0166 0.0092 0.0584 0.0233 0.0178 0.0319 0.0236 0.0368 0.0203 0.027 0.0186 0.0139 0.0197 0.0141
Mitochondria 0.0065 0.0006 0.0029 0.0118 0.0134 0.0013 0.0129 0.0001 0.001 0.0016 0.0003 0.0114 0.0001 0.0004 0.0004 0.0003 0.0004 0.0004
None 0.0266 0.0263 0.0467 0.0257 0.0133 0.041 0.0529 0.0191 0.0361 0.0101 0.0081 0.0414 0.0082 0.0137 0.0157 0.0084 0.0097 0.0171
Nuclear Periphery 0.0102 0.0042 0.004 0.0061 0.0044 0.0092 0.0232 0.0066 0.0052 0.0026 0.0036 0.0026 0.0037 0.0037 0.0031 0.0044 0.0028 0.0018
Nucleolus 0.0012 0.0003 0.0002 0.0002 0.0002 0.0002 0.0039 0.0003 0.0002 0.0002 0.0003 0.0001 0.0001 0.0003 0.0001 0.0003 0.0002 0.0001
Nucleus 0.0073 0.0022 0.0013 0.0014 0.0014 0.0021 0.0062 0.0036 0.0012 0.0009 0.001 0.0007 0.0008 0.0016 0.0011 0.0017 0.0035 0.0011
Peroxisomes 0.0028 0.0003 0.0007 0.0019 0.0016 0.0003 0.0397 0.0001 0.0002 0.0006 0.0054 0.001 0 0.0001 0.0001 0.0001 0.0002 0.0001
Punctate Nuclear 0.0021 0.001 0.0007 0.0017 0.0005 0.0009 0.0257 0.0007 0.001 0.0007 0.0216 0.0005 0.0002 0.0007 0.0003 0.0005 0.0008 0.0005
Vacuole 0.0096 0.009 0.0082 0.0097 0.0087 0.0066 0.0059 0.0218 0.0074 0.0089 0.0065 0.0071 0.0088 0.0108 0.0076 0.0208 0.0063 0.0054
Vacuole Periphery 0.0016 0.0004 0.0006 0.0008 0.0009 0.0008 0.003 0.0003 0.0006 0.0007 0.0002 0.0003 0.0002 0.0002 0.0003 0.0004 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 103.153 117.794 123.019 133.9088 120.2243 90.4583 123.0113 123.5554 146.1555 159.6648
Translational Efficiency 1.23 1.3089 1.2432 1.2804 1.1959 1.515 1.2073 1.2448 1.0715 1.1177

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Regulatory subunit of the vacuolar transporter chaperone (VTC) complex; involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; VTC2 has a paralog, VTC3, that arose from the whole genome duplication
Localization
Cell Percentages ER (76%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Vtc2

Vtc2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Vtc2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available