Standard name | |
---|---|
Human Ortholog | |
Description | GPI inositol deacylase of the endoplasmic reticulum (ER); negatively regulates COPII vesicle formation; prevents production of vesicles with defective subunits; required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules; functional ortholog of human PGAP1, mutation of which is associated with intellectual disability and encephalopathy |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 1 | 5 | 2 | 2 | 1 | 1 | 2 | – | 1 | 1 | 0 | 0 | 0 | 2 | – | 0 | 0 | 1 | 4 | 7 | 0 | 1 | 5 |
Bud | 0 | 4 | 4 | 5 | 3 | 10 | 9 | 25 | – | 27 | 22 | 28 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 1 | 0 | 5 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 5 | 2 | 1 | 3 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | – | 0 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 2 | 3 | 3 | 1 | 1 | – | 5 | 4 | 3 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 290 | 212 | 240 | 197 | 252 | 385 | 483 | 614 | – | 518 | 412 | 480 | 38 | 38 | 170 | – | 34 | 14 | 52 | 77 | 27 | 35 | 108 | 82 |
Endoplasmic Reticulum | 19 | 7 | 12 | 7 | 3 | 4 | 7 | 4 | – | 2 | 1 | 1 | 1 | 1 | 3 | – | 1 | 1 | 111 | 57 | 132 | 47 | 83 | 100 |
Endosome | 20 | 4 | 28 | 18 | 22 | 27 | 17 | 26 | – | 6 | 1 | 9 | 1 | 1 | 3 | – | 1 | 1 | 22 | 22 | 30 | 12 | 11 | 10 |
Golgi | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 3 | 3 | 12 | 1 | 2 | 2 |
Mitochondria | 2 | 5 | 6 | 5 | 9 | 53 | 70 | 137 | – | 176 | 148 | 198 | 2 | 2 | 0 | – | 1 | 0 | 36 | 15 | 44 | 29 | 36 | 24 |
Nucleus | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 5 | – | 2 | 1 | 3 | 1 | 1 | 0 | – | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | – | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 0 | 3 | 1 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 3 | – | 3 | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 |
SpindlePole | 2 | 0 | 7 | 1 | 4 | 4 | 5 | 7 | – | 4 | 5 | 4 | 0 | 0 | 2 | – | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 0 |
Vac/Vac Membrane | 22 | 7 | 20 | 6 | 24 | 19 | 17 | 15 | – | 16 | 14 | 24 | 0 | 0 | 2 | – | 6 | 3 | 162 | 83 | 82 | 75 | 144 | 176 |
Unique Cell Count | 341 | 231 | 298 | 228 | 307 | 455 | 565 | 749 | 631 | 499 | 599 | 43 | 43 | 176 | 43 | 18 | 398 | 278 | 352 | 210 | 407 | 416 | ||
Labelled Cell Count | 360 | 241 | 323 | 244 | 330 | 507 | 614 | 842 | 763 | 611 | 752 | 43 | 43 | 182 | 45 | 20 | 398 | 278 | 352 | 210 | 407 | 416 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 5.6 | 5.2 | 5.3 | 5.2 | 4.2 | 4.1 | 4.1 | – | 3.4 | 3.5 | 3.4 | 5.4 | 5.4 | 6.6 | 4.8 | 5.5 | 6.4 | 6.7 | 6.8 | 7.0 |
Std Deviation (1e-4) | 0.7 | 1.1 | 1.5 | 1.4 | 1.8 | 1.2 | 1.1 | 1.3 | – | 1.1 | 0.8 | 0.9 | 1.0 | 1.0 | 1.5 | 1.0 | 1.4 | 1.3 | 1.2 | 1.5 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.02 | -0.29 | -0.34 | -0.34 | – | -0.6 | -0.55 | -0.63 | 0.05 | 0.05 | 0.34 | -0.1 | 0.08 | 0.3 | 0.36 | 0.39 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cytoplasm | 1.8 | 0.5 | 1.5 | 1.9 | 0.5 | – | 0.6 | 0.7 | -0.1 | 1.2 | 1.2 | 4.9 | – | -0.2 | -0.3 |
Endoplasmic Reticulum | -0.6 | -2.4 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -1.4 | – | 0 | 0 |
Endosome | -0.6 | -1.0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -3.3 | – | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Mitochondria | 0 | 0.7 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
SpindlePole | 0 | -1.0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Vacuole | -2.1 | 0.5 | -1.5 | -2.6 | -3.8 | – | -3.1 | -2.6 | -1.8 | 0 | 0 | -2.8 | – | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.3322 | 1.6508 | 1.4507 | 1.5757 | 1.9094 | 1.39 | 1.8994 | 2.7634 | 2.553 | 2.2454 | 2.4187 | 2.3644 | 2.5651 | 2.0997 | 2.3453 | 1.8154 | 2.1117 | 2.0801 |
Actin | 0.0258 | 0.001 | 0.0083 | 0.0789 | 0.0526 | 0.0063 | 0.0346 | 0.0008 | 0.0142 | 0.003 | 0.0155 | 0.0085 | 0.0023 | 0.0003 | 0.0296 | 0.0149 | 0.0011 | 0.0021 |
Bud | 0.0007 | 0.0004 | 0.0005 | 0.0007 | 0.0008 | 0.0001 | 0.0006 | 0.0004 | 0.0004 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0003 | 0.0002 | 0.0003 | 0.0001 |
Bud Neck | 0.0036 | 0.0002 | 0.0019 | 0.0023 | 0.0018 | 0.0007 | 0.0018 | 0.0002 | 0.0009 | 0.0018 | 0.0007 | 0.0016 | 0.0004 | 0.0002 | 0.0003 | 0.0003 | 0.0012 | 0.0006 |
Bud Periphery | 0.0012 | 0.0006 | 0.0007 | 0.0009 | 0.001 | 0.0002 | 0.0006 | 0.0004 | 0.0005 | 0.0006 | 0.0001 | 0.0003 | 0.0001 | 0 | 0.0002 | 0.0002 | 0.0002 | 0.0001 |
Bud Site | 0.0054 | 0.0018 | 0.0018 | 0.0012 | 0.0038 | 0.0002 | 0.0041 | 0.0022 | 0.0055 | 0.0008 | 0.0004 | 0.0005 | 0.0003 | 0.0003 | 0.0008 | 0.0003 | 0.001 | 0.0001 |
Cell Periphery | 0.0009 | 0.0005 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0005 | 0.0006 | 0.0006 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0004 | 0.0001 | 0.0002 |
Cytoplasm | 0.314 | 0.4714 | 0.4837 | 0.2948 | 0.4133 | 0.5002 | 0.2395 | 0.4741 | 0.3599 | 0.2562 | 0.2449 | 0.4055 | 0.1376 | 0.2858 | 0.3843 | 0.4408 | 0.3872 | 0.4231 |
Cytoplasmic Foci | 0.0802 | 0.0695 | 0.0875 | 0.1156 | 0.1067 | 0.0743 | 0.1454 | 0.1272 | 0.118 | 0.1446 | 0.1811 | 0.1284 | 0.0243 | 0.0448 | 0.0794 | 0.0817 | 0.1232 | 0.0835 |
Eisosomes | 0.0005 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0406 | 0.0529 | 0.0411 | 0.0343 | 0.0202 | 0.0371 | 0.0563 | 0.031 | 0.0548 | 0.0117 | 0.0076 | 0.0495 | 0.1061 | 0.077 | 0.0451 | 0.0707 | 0.0306 | 0.073 |
Endosome | 0.2131 | 0.1382 | 0.175 | 0.2686 | 0.1955 | 0.1406 | 0.3256 | 0.1461 | 0.2195 | 0.3761 | 0.3689 | 0.2026 | 0.2891 | 0.2272 | 0.1901 | 0.1207 | 0.2392 | 0.1382 |
Golgi | 0.0176 | 0.0076 | 0.0141 | 0.0432 | 0.0344 | 0.0056 | 0.0466 | 0.0144 | 0.0204 | 0.0222 | 0.032 | 0.0135 | 0.0102 | 0.0082 | 0.0285 | 0.0244 | 0.0077 | 0.0085 |
Lipid Particles | 0.0316 | 0.0085 | 0.0107 | 0.0124 | 0.0328 | 0.0118 | 0.0406 | 0.0122 | 0.0225 | 0.0169 | 0.0125 | 0.0187 | 0.0318 | 0.0132 | 0.0216 | 0.0061 | 0.0533 | 0.0079 |
Mitochondria | 0.0093 | 0.0028 | 0.0047 | 0.0113 | 0.0087 | 0.005 | 0.0097 | 0.0084 | 0.0159 | 0.0196 | 0.0027 | 0.0052 | 0.0025 | 0.0006 | 0.0049 | 0.0027 | 0.0013 | 0.002 |
None | 0.1718 | 0.1906 | 0.1288 | 0.0683 | 0.0876 | 0.1833 | 0.05 | 0.1551 | 0.1286 | 0.0944 | 0.0792 | 0.1339 | 0.16 | 0.3112 | 0.1744 | 0.1752 | 0.1113 | 0.2393 |
Nuclear Periphery | 0.0295 | 0.0124 | 0.0085 | 0.0159 | 0.006 | 0.0065 | 0.0076 | 0.0028 | 0.0052 | 0.0024 | 0.0014 | 0.0048 | 0.0741 | 0.0078 | 0.0025 | 0.0156 | 0.0053 | 0.0042 |
Nucleolus | 0.0019 | 0.0007 | 0.0005 | 0.0003 | 0.0007 | 0.0005 | 0.0011 | 0.0002 | 0.0003 | 0.0003 | 0.0002 | 0.0002 | 0.0064 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 |
Nucleus | 0.0127 | 0.0047 | 0.004 | 0.0033 | 0.0021 | 0.0034 | 0.0017 | 0.002 | 0.0015 | 0.0012 | 0.0019 | 0.0013 | 0.0875 | 0.001 | 0.0008 | 0.0023 | 0.0021 | 0.0016 |
Peroxisomes | 0.0042 | 0.0009 | 0.0067 | 0.0171 | 0.008 | 0.0022 | 0.0139 | 0.0039 | 0.0117 | 0.0054 | 0.0332 | 0.0101 | 0.0007 | 0.0003 | 0.0241 | 0.0033 | 0.0066 | 0.0014 |
Punctate Nuclear | 0.0083 | 0.0037 | 0.005 | 0.006 | 0.0084 | 0.0066 | 0.0058 | 0.0014 | 0.0027 | 0.0023 | 0.0014 | 0.0024 | 0.0417 | 0.0007 | 0.0014 | 0.0007 | 0.002 | 0.0019 |
Vacuole | 0.0215 | 0.0283 | 0.0136 | 0.0202 | 0.0121 | 0.0129 | 0.0116 | 0.015 | 0.0145 | 0.0328 | 0.0139 | 0.0105 | 0.0203 | 0.0194 | 0.0088 | 0.0354 | 0.022 | 0.0106 |
Vacuole Periphery | 0.0058 | 0.0034 | 0.0026 | 0.0042 | 0.0031 | 0.0023 | 0.0022 | 0.0015 | 0.0024 | 0.0068 | 0.0022 | 0.0022 | 0.0038 | 0.0015 | 0.0024 | 0.0039 | 0.0042 | 0.0018 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.6046 | 8.938 | 9.4749 | 9.1913 | 14.5839 | 14.9894 | 12.2648 | 10.4563 | 8.1303 | 17.1051 |
Translational Efficiency | 0.5373 | 0.611 | 0.4186 | 0.3536 | 0.3883 | 0.5906 | 0.4765 | 0.3406 | 0.4945 | 0.3803 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
479 | 524 | 215 | 81 | 836 | 1391 | 2172 | 201 | 1315 | 1915 | 2387 | 282 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 824.84 | 851.95 | 971.33 | 1008.36 | 582.48 | 816.71 | 842.66 | 905.79 | 670.76 | 826.35 | 854.25 | 935.25 |
Standard Deviation | 87.33 | 128.43 | 95.79 | 124.57 | 71.76 | 110.07 | 106.87 | 104.09 | 140.19 | 116.45 | 112.14 | 119.72 |
Intensity Change Log 2 | — | 0.046654 | 0.235847 | 0.289825 | — | 0.487615 | 0.532742 | 0.636968 | — | 0.245740 | 0.366216 | 0.443753 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000658 | 0.002577 | 0.001911 | 0.000457 | 0.000760 | 0.000843 | 0.000533 | 0.000294 | 0.000723 | 0.001318 | 0.000657 | 0.000341 |
Bud Neck | 0.005106 | 0.008536 | 0.004137 | 0.002684 | 0.025352 | 0.008839 | 0.009618 | 0.002288 | 0.017977 | 0.008756 | 0.009125 | 0.002402 |
Bud Site | 0.008772 | 0.007981 | 0.014052 | 0.005060 | 0.006033 | 0.006931 | 0.015718 | 0.008635 | 0.007030 | 0.007218 | 0.015568 | 0.007608 |
Cell Periphery | 0.000238 | 0.000157 | 0.000449 | 0.000306 | 0.000321 | 0.000183 | 0.000187 | 0.000256 | 0.000291 | 0.000176 | 0.000210 | 0.000270 |
Cytoplasm | 0.330854* | 0.137495* | 0.331053* | 0.268363* | 0.222504* | 0.144671* | 0.456775* | 0.293442* | 0.261971* | 0.142707* | 0.445451* | 0.286239* |
Cytoplasmic Foci | 0.132348 | 0.367389* | 0.129960 | 0.066686 | 0.387451* | 0.277681* | 0.115062 | 0.141766 | 0.294527* | 0.302228* | 0.116404 | 0.120201 |
Eisosomes | 0.000162 | 0.000375 | 0.000139 | 0.000155 | 0.001284 | 0.000279 | 0.000119 | 0.000187 | 0.000875 | 0.000306 | 0.000121 | 0.000178 |
Endoplasmic Reticulum | 0.012396 | 0.028449 | 0.026872 | 0.013034 | 0.006270 | 0.031832 | 0.028623 | 0.012480 | 0.008501 | 0.030907 | 0.028465 | 0.012640 |
Endosome | 0.035679 | 0.115913 | 0.036744 | 0.010946 | 0.078712 | 0.139862 | 0.020104 | 0.028885 | 0.063037 | 0.133309 | 0.021603 | 0.023732 |
Golgi | 0.003090 | 0.019176 | 0.001317 | 0.000116 | 0.054582 | 0.028556 | 0.001147 | 0.000475 | 0.035826 | 0.025990 | 0.001163 | 0.000372 |
Lipid Particles | 0.026621 | 0.052170 | 0.020920 | 0.010017 | 0.092822 | 0.067570 | 0.037168 | 0.021430 | 0.068708 | 0.063356 | 0.035704 | 0.018152 |
Mitochondria | 0.004939 | 0.002898 | 0.000391 | 0.000077 | 0.019587 | 0.009525 | 0.001431 | 0.000216 | 0.014252 | 0.007712 | 0.001337 | 0.000176 |
Mitotic Spindle | 0.006265 | 0.006180 | 0.002808 | 0.000177 | 0.000323 | 0.005543 | 0.011064 | 0.003598 | 0.002487 | 0.005717 | 0.010321 | 0.002615 |
None | 0.003368 | 0.000197 | 0.000867 | 0.000532 | 0.003486 | 0.000687 | 0.001853 | 0.002484 | 0.003443 | 0.000553 | 0.001765 | 0.001923 |
Nuclear Periphery | 0.007574 | 0.000721 | 0.003999 | 0.002622 | 0.000406 | 0.002659 | 0.012535 | 0.004081 | 0.003017 | 0.002129 | 0.011766 | 0.003662 |
Nuclear Periphery Foci | 0.010376 | 0.003650 | 0.071126 | 0.022138 | 0.000506 | 0.002005 | 0.019850 | 0.023362 | 0.004101 | 0.002455 | 0.024469 | 0.023011 |
Nucleolus | 0.000922 | 0.000459 | 0.000744 | 0.000180 | 0.001232 | 0.000906 | 0.001508 | 0.001177 | 0.001119 | 0.000784 | 0.001439 | 0.000891 |
Nucleus | 0.109160 | 0.009262 | 0.121463 | 0.060319 | 0.026872 | 0.020508 | 0.151408* | 0.095832 | 0.056846 | 0.017431 | 0.148710* | 0.085631 |
Peroxisomes | 0.005529 | 0.023020 | 0.003397 | 0.000643 | 0.021434 | 0.011644 | 0.002637 | 0.001266 | 0.015641 | 0.014757 | 0.002705 | 0.001087 |
Vacuole | 0.291830* | 0.209648* | 0.226738* | 0.535356* | 0.045696 | 0.229471* | 0.111989 | 0.357419* | 0.135352 | 0.224047* | 0.122324 | 0.408529* |
Vacuole Periphery | 0.004115 | 0.003746 | 0.000912 | 0.000131 | 0.004367 | 0.009805 | 0.000670 | 0.000425 | 0.004275 | 0.008147 | 0.000692 | 0.000341 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.76 | -2.56 | 0.52 | 3.03 | 3.02 | -0.40 | 1.19 | 3.85 | 2.45 | 0.96 | -2.40 | 0.32 | 3.08 | 3.88 | 1.44 |
Bud Neck | -2.30 | 0.98 | 2.16 | 3.93 | 1.15 | 8.02 | 7.25 | 11.58 | 10.93 | 10.16 | 6.54 | 5.76 | 11.60 | 10.82 | 9.86 |
Bud Site | 0.08 | -1.36 | 1.16 | 1.67 | 2.18 | -0.73 | -4.89 | -0.73 | -0.42 | 1.76 | -0.37 | -4.45 | -0.34 | -0.16 | 2.53 |
Cell Periphery | 2.47 | -5.80 | -0.96 | -2.72 | 3.78 | 3.58 | 5.62 | 2.96 | -1.49 | -2.97 | 3.89 | 4.34 | 1.82 | -2.46 | -2.22 |
Cytoplasm | 13.06 | 0.02 | 3.28 | -3.05 | 3.06 | 8.82 | -23.91 | -3.91 | -8.53 | 8.63 | 15.13 | -20.69 | -0.97 | -8.76 | 10.48 |
Cytoplasmic Foci | -21.23 | 0.80 | 9.76 | 29.26 | 6.45 | 12.54 | 34.83 | 24.10 | 15.79 | -0.73 | -1.06 | 25.99 | 21.54 | 25.20 | 2.16 |
Eisosomes | -2.06 | 1.48 | 1.09 | 2.23 | 0.05 | 11.80 | 13.92 | 13.23 | 5.34 | -3.75 | 9.07 | 13.54 | 12.74 | 4.50 | -3.50 |
Endoplasmic Reticulum | -3.60 | -4.07 | 1.01 | 3.89 | 4.28 | -9.56 | -10.84 | -2.03 | 6.52 | 6.07 | -9.76 | -10.59 | -1.12 | 7.62 | 7.35 |
Endosome | -13.01 | -0.08 | 7.28 | 20.58 | 6.31 | -10.82 | 14.68 | 12.18 | 25.08 | -2.39 | -16.19 | 13.75 | 13.03 | 30.97 | 0.65 |
Golgi | -7.58 | 1.61 | 2.93 | 9.76 | 2.67 | 6.84 | 16.47 | 16.69 | 13.83 | 3.19 | 3.68 | 15.70 | 16.08 | 16.26 | 4.14 |
Lipid Particles | -5.22 | 2.03 | 6.98 | 9.87 | 5.60 | 4.87 | 11.56 | 12.79 | 11.02 | 4.52 | 1.47 | 9.53 | 12.98 | 13.87 | 6.36 |
Mitochondria | 0.83 | 2.62 | 2.81 | 2.59 | 2.67 | 4.71 | 9.35 | 10.04 | 9.80 | 5.03 | 4.12 | 9.23 | 10.18 | 10.00 | 5.31 |
Mitotic Spindle | -0.23 | 0.78 | 2.10 | 2.54 | 2.57 | -4.22 | -7.43 | -1.76 | 0.99 | 3.32 | -2.34 | -4.84 | -0.09 | 1.98 | 4.28 |
None | 1.48 | 1.11 | 1.29 | -2.00 | 1.49 | 2.18 | 1.34 | 0.85 | -1.69 | -0.47 | 2.59 | 1.50 | 1.30 | -1.83 | -0.05 |
Nuclear Periphery | 5.33 | 2.66 | 4.04 | -2.62 | 2.49 | -4.35 | -12.90 | -3.14 | -1.07 | 5.62 | 1.38 | -8.89 | -0.49 | -1.42 | 6.84 |
Nuclear Periphery Foci | 4.07 | -8.74 | -1.79 | -4.16 | 7.32 | -6.52 | -16.88 | -6.25 | -5.80 | -0.44 | 2.64 | -14.93 | -6.11 | -6.89 | 1.54 |
Nucleolus | 3.44 | 1.38 | 6.07 | 5.65 | 6.40 | 1.62 | -1.33 | 0.75 | -0.42 | 1.94 | 2.33 | -1.79 | 1.56 | 0.05 | 2.94 |
Nucleus | 15.25 | -1.34 | 5.40 | -6.02 | 5.53 | 2.16 | -29.16 | -7.31 | -8.34 | 6.55 | 12.14 | -21.15 | -3.51 | -9.72 | 9.41 |
Peroxisomes | -5.90 | 1.35 | 3.34 | 8.48 | 3.38 | 5.80 | 13.29 | 14.62 | 11.22 | 5.72 | 0.55 | 11.94 | 13.96 | 13.82 | 7.12 |
Vacuole | 6.46 | 3.25 | -8.97 | -12.15 | -10.49 | -27.64 | -18.45 | -19.24 | -8.64 | -14.81 | -10.61 | 1.02 | -17.70 | -13.22 | -18.80 |
Vacuole Periphery | -0.03 | 2.06 | 2.61 | 10.56 | 3.28 | -7.89 | 11.17 | 12.01 | 15.61 | 3.07 | -5.74 | 6.45 | 7.11 | 17.30 | 4.84 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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