Standard name | |
---|---|
Human Ortholog | |
Description | Receptor for alpha-factor pheromone; seven transmembrane-domain GPCR that interacts with both pheromone and a heterotrimeric G protein to initiate the signaling response that leads to mating between haploid a and alpha cells |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 1 | 4 | 4 | 2 | 4 | 2 | 0 | 2 | 0 | 2 | 2 | 17 | 22 | 22 | 0 | 0 | 0 | 0 | 0 | 6 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 2 | 1 | 3 | 0 | 0 | 0 | 2 | 0 | 3 | 1 | 1 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 7 | 1 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 2 | 15 | 9 | 8 | 25 | 29 | 43 | 55 | 46 | 54 | 55 | 11 | 25 | 50 | 6 | 5 | 4 | 2 | 0 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 7 | 0 | 27 | 14 | 4 | 6 | 3 | 3 | 3 | 0 | 5 | 1 | 75 | 65 | 24 | 2 | 6 | 2 | 9 | 2 | 40 | 0 | 0 | 1 |
Endosome | 1 | 0 | 1 | 2 | 3 | 14 | 1 | 2 | 1 | 1 | 1 | 2 | 2 | 11 | 22 | 2 | 3 | 2 | 3 | 0 | 1 | 22 | 12 | 29 |
Golgi | 11 | 0 | 53 | 23 | 15 | 1 | 7 | 3 | 5 | 1 | 0 | 2 | 53 | 62 | 16 | 0 | 0 | 2 | 4 | 0 | 4 | 10 | 6 | 17 |
Mitochondria | 13 | 4 | 12 | 6 | 14 | 23 | 39 | 48 | 46 | 49 | 60 | 48 | 0 | 2 | 1 | 0 | 4 | 3 | 6 | 0 | 9 | 20 | 37 | 35 |
Nucleus | 30 | 3 | 27 | 49 | 38 | 91 | 172 | 186 | 72 | 87 | 54 | 71 | 10 | 44 | 45 | 4 | 20 | 9 | 6 | 2 | 0 | 8 | 30 | 16 |
Nuclear Periphery | 3 | 0 | 2 | 0 | 0 | 6 | 9 | 6 | 4 | 8 | 5 | 6 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 2 | 7 | 22 | 35 |
Nucleolus | 14 | 0 | 11 | 24 | 10 | 22 | 14 | 26 | 8 | 8 | 17 | 17 | 1 | 3 | 6 | 0 | 0 | 0 | 1 | 0 | 0 | 23 | 35 | 28 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
SpindlePole | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 12 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 2 |
Vac/Vac Membrane | 90 | 10 | 171 | 178 | 230 | 333 | 482 | 642 | 344 | 328 | 360 | 381 | 78 | 180 | 141 | 13 | 40 | 27 | 148 | 53 | 249 | 243 | 366 | 338 |
Unique Cell Count | 145 | 20 | 261 | 263 | 288 | 467 | 692 | 840 | 467 | 460 | 473 | 500 | 206 | 363 | 305 | 25 | 59 | 38 | 190 | 63 | 319 | 343 | 520 | 516 |
Labelled Cell Count | 177 | 20 | 323 | 312 | 326 | 529 | 759 | 961 | 540 | 529 | 559 | 585 | 256 | 426 | 352 | 28 | 78 | 49 | 190 | 63 | 319 | 343 | 520 | 516 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 26.0 | 15.0 | 28.0 | 32.7 | 39.5 | 34.2 | 35.0 | 33.0 | 33.8 | 32.4 | 29.4 | 31.9 | 23.0 | 15.5 | 11.6 | 6.1 | 6.6 | 8.1 | 32.5 | 30.7 | 32.1 |
Std Deviation (1e-4) | 8.9 | 7.1 | 10.6 | 11.3 | 17.5 | 18.0 | 18.5 | 17.8 | 16.8 | 17.5 | 15.6 | 16.5 | 7.4 | 4.9 | 4.2 | 1.2 | 1.6 | 2.1 | 10.9 | 10.9 | 11.6 |
Intensity Change (Log2) | – | – | – | 0.23 | 0.5 | 0.29 | 0.33 | 0.24 | 0.27 | 0.21 | 0.07 | 0.19 | -0.28 | -0.85 | -1.27 | -2.19 | -2.07 | -1.79 | 0.22 | 0.13 | 0.2 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 2.8 | 3.2 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.3 | -1.7 | -0.2 | -1.0 | -0.4 | 2.6 | 2.0 | 2.5 | 2.4 | -0.2 | 0.6 | 4.0 | 0 | 0 | 0 |
Endoplasmic Reticulum | -2.1 | -4.5 | -5.6 | -7.8 | -8.7 | -6.3 | -7.0 | -5.9 | -7.1 | 6.8 | 2.6 | -1.0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 4.1 | 0 | 0 | 0 |
Golgi | -3.8 | -5.4 | -9.9 | -10.9 | -12.8 | -9.2 | -9.8 | -10.2 | -10.1 | 1.4 | -1.0 | -5.5 | 0 | -3.8 | -2.2 |
Mitochondria | -1.5 | 0.1 | 0.2 | 0.6 | 0.7 | 2.5 | 2.8 | 3.5 | 2.4 | -3.1 | -3.4 | -3.4 | 0 | 0 | 0 |
Nucleus | 2.7 | 1.0 | 3.2 | 4.9 | 4.2 | 1.9 | 3.0 | 0.4 | 1.5 | -2.2 | 0.7 | 1.6 | 0 | 4.6 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2.3 | -0.5 | 0.3 | -1.9 | -0.9 | -2.0 | -2.0 | -0.4 | -0.6 | -2.5 | -2.8 | -1.6 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 | 0 |
Vacuole | 0.5 | 3.8 | 1.6 | 1.2 | 3.5 | 2.3 | 1.6 | 3.1 | 3.1 | -5.9 | -4.0 | -4.6 | -1.3 | 0.3 | 0.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 31.6783 | 31.8271 | 28.0587 | 29.1503 | 27.9609 | 29.7961 | 32.9923 | 34.4411 | 27.9314 | 27.2646 | 24.2402 | 30.0409 | 31.1079 | 35.7416 | 33.828 | 27.8262 | 37.1343 | 30.5432 |
Actin | 0.0062 | 0.0001 | 0.0069 | 0 | 0 | 0.0002 | 0.0197 | 0.0001 | 0.0003 | 0.0001 | 0.0321 | 0.0001 | 0.0059 | 0.0006 | 0.006 | 0 | 0 | 0.0019 |
Bud | 0.0003 | 0.0002 | 0.0003 | 0.0003 | 0.0003 | 0.0003 | 0.0006 | 0.0005 | 0.0006 | 0.0002 | 0.0016 | 0.0003 | 0.0008 | 0.0006 | 0.0006 | 0.0002 | 0.0003 | 0.0013 |
Bud Neck | 0.001 | 0.0005 | 0.001 | 0.0008 | 0.0005 | 0.0005 | 0.0019 | 0.0006 | 0.0015 | 0.0004 | 0.0014 | 0.0007 | 0.0008 | 0.0023 | 0.0014 | 0.0007 | 0.0016 | 0.0014 |
Bud Periphery | 0.0012 | 0.0009 | 0.0013 | 0.0012 | 0.0012 | 0.0014 | 0.0022 | 0.0017 | 0.0029 | 0.0011 | 0.004 | 0.0022 | 0.0025 | 0.0026 | 0.0026 | 0.0008 | 0.0011 | 0.0076 |
Bud Site | 0.0008 | 0.0005 | 0.0011 | 0.0007 | 0.0004 | 0.0003 | 0.0034 | 0.0021 | 0.005 | 0.0004 | 0.0114 | 0.0004 | 0.0018 | 0.0025 | 0.0017 | 0.0003 | 0.0038 | 0.0012 |
Cell Periphery | 0.2053 | 0.1547 | 0.1531 | 0.1071 | 0.116 | 0.1441 | 0.2695 | 0.2434 | 0.218 | 0.1968 | 0.1528 | 0.2094 | 0.1875 | 0.169 | 0.1646 | 0.1425 | 0.2132 | 0.1689 |
Cytoplasm | 0.0019 | 0.0006 | 0.0049 | 0.0005 | 0.001 | 0.0007 | 0.0046 | 0.0009 | 0.0016 | 0.0004 | 0.0013 | 0.0006 | 0.0036 | 0.0005 | 0.0004 | 0.0003 | 0.0004 | 0.0018 |
Cytoplasmic Foci | 0.0028 | 0.0005 | 0.0007 | 0.0004 | 0.0003 | 0.0005 | 0.0079 | 0.0004 | 0.0042 | 0.0009 | 0.006 | 0.0013 | 0.0102 | 0.0016 | 0.0048 | 0.0009 | 0.0018 | 0.0016 |
Eisosomes | 0.0003 | 0 | 0.0002 | 0 | 0 | 0 | 0.0004 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.0003 | 0.0002 | 0.0003 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0021 | 0.0024 | 0.0134 | 0.0004 | 0.0008 | 0.0028 | 0.0051 | 0.007 | 0.0041 | 0.0013 | 0.0022 | 0.0029 | 0.0062 | 0.0028 | 0.0012 | 0.0004 | 0.0004 | 0.0022 |
Endosome | 0.0103 | 0.0077 | 0.0103 | 0.0058 | 0.0091 | 0.0139 | 0.0123 | 0.0102 | 0.01 | 0.0095 | 0.0348 | 0.0125 | 0.0365 | 0.0221 | 0.0093 | 0.0151 | 0.0179 | 0.0235 |
Golgi | 0.0011 | 0.0008 | 0.0023 | 0.0003 | 0.0002 | 0.0034 | 0.0034 | 0.0007 | 0.0013 | 0.0009 | 0.0037 | 0.0047 | 0.0157 | 0.0059 | 0.0048 | 0.0006 | 0.0021 | 0.0081 |
Lipid Particles | 0.033 | 0.0123 | 0.0206 | 0.0329 | 0.0065 | 0.016 | 0.0538 | 0.0191 | 0.0375 | 0.0681 | 0.0511 | 0.0191 | 0.0683 | 0.0256 | 0.08 | 0.0694 | 0.083 | 0.0276 |
Mitochondria | 0.0055 | 0.0019 | 0.0142 | 0.0008 | 0.0003 | 0.0043 | 0.016 | 0.0115 | 0.0189 | 0.0025 | 0.0215 | 0.007 | 0.0702 | 0.018 | 0.0557 | 0.0007 | 0.0015 | 0.0184 |
None | 0.0013 | 0 | 0.0003 | 0 | 0 | 0 | 0.0024 | 0.0001 | 0.0036 | 0 | 0.0002 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
Nuclear Periphery | 0.0024 | 0.0028 | 0.0031 | 0.0004 | 0.0006 | 0.0016 | 0.0051 | 0.0023 | 0.0023 | 0.0007 | 0.0011 | 0.0014 | 0.0021 | 0.0016 | 0.0008 | 0.0004 | 0.0004 | 0.0013 |
Nucleolus | 0.0295 | 0.009 | 0.0048 | 0.0099 | 0.0044 | 0.0041 | 0.0077 | 0.0035 | 0.017 | 0.0051 | 0.0195 | 0.0116 | 0.0151 | 0.0185 | 0.0126 | 0.0111 | 0.0316 | 0.0097 |
Nucleus | 0.0096 | 0.0031 | 0.0025 | 0.0026 | 0.0019 | 0.002 | 0.0025 | 0.0017 | 0.0034 | 0.0014 | 0.0033 | 0.0026 | 0.0043 | 0.0037 | 0.003 | 0.0025 | 0.0033 | 0.0029 |
Peroxisomes | 0.0005 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0033 | 0.0001 | 0.0009 | 0.0001 | 0.0024 | 0.0002 | 0.0035 | 0.0013 | 0.0057 | 0.0002 | 0.0005 | 0.0004 |
Punctate Nuclear | 0.0008 | 0 | 0.0002 | 0 | 0 | 0 | 0.0027 | 0 | 0.0007 | 0 | 0.0004 | 0 | 0.0015 | 0 | 0 | 0 | 0 | 0.0001 |
Vacuole | 0.5819 | 0.6791 | 0.6291 | 0.6952 | 0.7642 | 0.6602 | 0.4948 | 0.5852 | 0.5546 | 0.5996 | 0.583 | 0.6179 | 0.4351 | 0.5516 | 0.4746 | 0.6248 | 0.5552 | 0.5984 |
Vacuole Periphery | 0.1023 | 0.1228 | 0.1293 | 0.1404 | 0.0921 | 0.1437 | 0.0806 | 0.1088 | 0.1113 | 0.1104 | 0.0659 | 0.1052 | 0.1276 | 0.1689 | 0.1698 | 0.1288 | 0.0815 | 0.1218 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 60.0232 | 39.5768 | 140.0614 | 225.0721 | 91.0691 | 77.4338 | 69.6434 | 111.6684 | 145.7249 | 115.1523 |
Translational Efficiency | 0.547 | 0.4934 | 0.8881 | 0.5523 | 0.7701 | 0.5996 | 0.4755 | 0.6262 | 0.759 | 0.678 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
99 | 1138 | 1186 | 829 | 1659 | 976 | 2503 | 106 | 1758 | 2114 | 3689 | 935 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1000.12 | 2202.31 | 1288.02 | 1009.26 | 1485.12 | 2050.54 | 1226.35 | 1037.72 | 1457.81 | 2132.24 | 1246.18 | 1012.49 |
Standard Deviation | 141.37 | 735.13 | 277.67 | 227.08 | 344.18 | 627.15 | 274.00 | 156.26 | 354.14 | 691.54 | 276.69 | 220.38 |
Intensity Change Log 2 | — | 1.138844 | 0.364982 | 0.013125 | — | 0.465424 | -0.276209 | -0.517162 | — | 0.775045 | 0.016812 | -0.279888 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.013164 | 0.001360 | 0.017183 | 0.037971 | 0.000172 | 0.001159 | 0.030851 | 0.035025 | 0.000904 | 0.001267 | 0.026457 | 0.037637 |
Bud Neck | 0.037998 | 0.013792 | 0.047490 | 0.048722 | 0.007927 | 0.014908 | 0.093128 | 0.025610 | 0.009620 | 0.014307 | 0.078456 | 0.046102 |
Bud Site | 0.057436 | 0.022121 | 0.128557* | 0.163104* | 0.005557 | 0.015221 | 0.194389* | 0.072209 | 0.008479 | 0.018935 | 0.173224* | 0.152800* |
Cell Periphery | 0.048238 | 0.271339* | 0.080754 | 0.049251 | 0.310399* | 0.254504* | 0.124821* | 0.033490 | 0.295636* | 0.263567* | 0.110654* | 0.047464 |
Cytoplasm | 0.065516 | 0.001415 | 0.025118 | 0.116646* | 0.000481 | 0.001210 | 0.042471 | 0.104415* | 0.004143 | 0.001321 | 0.036892 | 0.115259* |
Cytoplasmic Foci | 0.038306 | 0.001352 | 0.016651 | 0.053178 | 0.000139 | 0.002012 | 0.016381 | 0.061187 | 0.002288 | 0.001656 | 0.016468 | 0.054086 |
Eisosomes | 0.001509 | 0.001869 | 0.000693 | 0.001856 | 0.000421 | 0.003089 | 0.001621 | 0.001696 | 0.000482 | 0.002432 | 0.001323 | 0.001838 |
Endoplasmic Reticulum | 0.019126 | 0.025793 | 0.056156 | 0.015125 | 0.037540 | 0.025318 | 0.046205 | 0.009307 | 0.036503 | 0.025574 | 0.049404 | 0.014466 |
Endosome | 0.103928 | 0.016185 | 0.118785 | 0.101987 | 0.007265 | 0.014257 | 0.122760 | 0.118448 | 0.012708 | 0.015295 | 0.121482 | 0.103853 |
Golgi | 0.016081 | 0.017218 | 0.016702 | 0.016623 | 0.001443 | 0.005280 | 0.016211 | 0.018882 | 0.002268 | 0.011706 | 0.016369 | 0.016879 |
Lipid Particles | 0.011503 | 0.010333 | 0.057479 | 0.030966 | 0.001206 | 0.004364 | 0.064183 | 0.014661 | 0.001786 | 0.007577 | 0.062028 | 0.029118 |
Mitochondria | 0.007271 | 0.002254 | 0.000878 | 0.001331 | 0.000213 | 0.002343 | 0.006793 | 0.013130 | 0.000611 | 0.002295 | 0.004891 | 0.002669 |
Mitotic Spindle | 0.009401 | 0.000084 | 0.000299 | 0.003610 | 0.000036 | 0.000452 | 0.000335 | 0.001392 | 0.000564 | 0.000254 | 0.000323 | 0.003359 |
None | 0.000024 | 0.000567 | 0.000036 | 0.000146 | 0.000022 | 0.000126 | 0.000080 | 0.000079 | 0.000022 | 0.000364 | 0.000066 | 0.000138 |
Nuclear Periphery | 0.000285 | 0.000396 | 0.000201 | 0.000204 | 0.000085 | 0.000352 | 0.000179 | 0.000567 | 0.000096 | 0.000376 | 0.000186 | 0.000245 |
Nuclear Periphery Foci | 0.002144 | 0.001114 | 0.003098 | 0.002299 | 0.000543 | 0.001209 | 0.001470 | 0.002327 | 0.000633 | 0.001158 | 0.001993 | 0.002302 |
Nucleolus | 0.000395 | 0.001001 | 0.000404 | 0.000179 | 0.000401 | 0.000807 | 0.000326 | 0.000132 | 0.000400 | 0.000912 | 0.000351 | 0.000174 |
Nucleus | 0.006040 | 0.000451 | 0.001260 | 0.007980 | 0.000166 | 0.000336 | 0.001685 | 0.017307 | 0.000496 | 0.000398 | 0.001548 | 0.009037 |
Peroxisomes | 0.006483 | 0.000346 | 0.002332 | 0.018871 | 0.000033 | 0.000388 | 0.006637 | 0.016120 | 0.000396 | 0.000366 | 0.005253 | 0.018559 |
Vacuole | 0.543918* | 0.562174* | 0.412167* | 0.324974* | 0.605693* | 0.624713* | 0.218383* | 0.442926* | 0.602214* | 0.591047* | 0.280684* | 0.338346* |
Vacuole Periphery | 0.011235 | 0.048835 | 0.013755 | 0.004978 | 0.020257 | 0.027953 | 0.011092 | 0.011092 | 0.019749 | 0.039194 | 0.011948 | 0.005671 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.92 | -0.87 | -2.75 | -8.22 | -3.33 | -4.79 | -17.04 | -2.57 | -2.48 | 0.35 | -1.23 | -18.48 | -8.69 | -8.59 | -1.00 |
Bud Neck | 2.76 | -1.46 | -0.62 | -7.92 | 1.63 | -5.37 | -28.77 | -2.61 | -1.22 | 12.25 | -4.56 | -30.19 | -9.54 | -7.96 | 10.95 |
Bud Site | 2.71 | -5.87 | -6.14 | -14.93 | -0.87 | -4.35 | -39.79* | -4.65 | -3.80 | 10.27 | -5.69 | -42.67 | -17.71 | -15.93 | 5.71 |
Cell Periphery | -13.33 | -2.21 | 0.39 | 24.88* | 6.40 | 4.91 | 22.90 | 20.17 | 15.09 | 7.51 | 3.51 | 24.67 | 32.16 | 30.75* | 13.62 |
Cytoplasm | 4.85 | 3.06 | -4.28 | -19.03 | -14.79 | -0.97 | -19.33 | -7.65 | -7.60 | -5.06 | 2.83 | -17.38 | -19.88 | -20.47 | -14.54 |
Cytoplasmic Foci | 5.19 | 3.04 | -1.11 | -14.45 | -8.95 | -2.80 | -16.09 | -7.55 | -7.26 | -5.26 | 1.19 | -14.92 | -15.47 | -15.78 | -10.58 |
Eisosomes | -0.63 | 0.99 | -0.10 | 1.08 | -2.66 | -8.03 | -12.16 | -1.59 | 2.68 | 0.47 | -9.85 | -9.96 | -3.62 | 2.97 | -0.56 |
Endoplasmic Reticulum | -1.10 | -4.80 | 0.74 | 5.64 | 11.51 | 4.22 | -2.87 | 10.18 | 5.50 | 11.93 | 4.75 | -4.86 | 11.03 | 6.98 | 16.08 |
Endosome | 4.92 | -1.23 | 0.84 | -11.58 | 4.86 | -4.33 | -31.49* | -4.70 | -4.30 | 1.56 | -1.87 | -34.70* | -12.99 | -12.59 | 6.09 |
Golgi | -0.46 | -0.33 | 0.35 | 1.43 | 1.45 | -3.44 | -14.27 | -1.71 | -1.20 | 0.20 | -6.97 | -15.51 | -5.20 | -0.59 | 1.40 |
Lipid Particles | 0.07 | -7.25 | -2.37 | -4.05 | 6.23 | -3.82 | -20.21 | -1.77 | -1.24 | 7.52 | -6.18 | -24.14 | -7.16 | -5.07 | 9.79 |
Mitochondria | 0.95 | 1.25 | 1.20 | 2.02 | -0.62 | -3.14 | -14.70 | -1.36 | -1.06 | -0.46 | -3.60 | -10.57 | -1.69 | 0.18 | 2.95 |
Mitotic Spindle | 1.08 | 1.05 | 0.58 | -3.64 | -3.43 | -1.88 | -10.60 | -2.21 | -1.86 | -1.96 | 0.57 | 0.39 | -3.17 | -3.73 | -3.66 |
None | -1.50 | -1.22 | -3.81 | 0.95 | -3.63 | -2.84 | -4.70 | -2.11 | -1.40 | -1.72 | -1.75 | -4.09 | -4.36 | 0.64 | -3.48 |
Nuclear Periphery | -0.94 | 0.64 | -0.25 | 1.55 | -3.11 | -3.95 | -8.55 | -2.87 | -1.58 | -2.43 | -6.76 | -7.79 | -7.34 | 0.36 | -4.67 |
Nuclear Periphery Foci | 1.48 | -1.55 | -0.43 | -2.35 | 1.14 | -5.71 | -6.73 | -3.84 | -2.44 | -1.76 | -6.53 | -11.23 | -3.57 | -2.55 | -0.79 |
Nucleolus | -2.67 | -0.26 | 0.93 | 5.04 | 1.26 | -3.98 | 1.81 | 5.10 | 6.69 | 6.06 | -5.02 | 0.53 | 4.51 | 7.37 | 2.58 |
Nucleus | 3.47 | 2.86 | -3.47 | -8.64 | -7.94 | -2.13 | -7.89 | -4.27 | -4.24 | -4.02 | 1.15 | -6.08 | -9.51 | -9.61 | -8.76 |
Peroxisomes | 1.82 | 1.19 | -2.58 | -7.31 | -6.37 | -1.84 | -12.93 | -3.75 | -3.64 | -1.94 | -0.01 | -12.19 | -7.93 | -7.94 | -5.34 |
Vacuole | -0.13 | 4.26 | 5.07 | 12.53 | 2.14 | -1.60 | 44.57* | 3.93 | 4.42 | -7.68 | 1.29 | 38.47* | 16.32 | 15.69 | -9.10 |
Vacuole Periphery | -8.69 | -0.90 | 1.78 | 16.44 | 9.47 | -2.99 | 5.98 | 2.37 | 3.75 | 0.52 | -8.69 | 5.37 | 9.63 | 18.25 | 8.55 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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