Standard name
Human Ortholog
Description Cyclin-dependent kinase-activating kinase; required for passage through the cell cycle; phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0.05 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.95 0.98 0.98 0.99 0.95 0.89 0.93 0.83 0.85 0.74 0.79 1.0 0.98 0.99 0.98 0.97 0.95 0.84 0.86 0.74 0.88 0.8 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 0 0 0 0.05 0.16 0.09 0.26 0.21 0.48 0.22 0 0 0 0 0 0 0 0 0.1 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 3 1 3 3
Bud 0 1 0 0 1 0 5 2 5 0 7 4 0 0 0 0 0 1 1 0 1 0 3 2
Bud Neck 2 1 5 4 13 7 3 17 5 7 2 11 1 3 0 0 0 0 0 2 1 0 2 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 3 3 1 0 0 1 1 2 1 5 6 0 1 3 4 4 0 0 3 0 1 1
Cytoplasm 158 135 175 202 285 256 313 227 249 209 192 206 137 206 175 122 170 130 131 128 118 85 144 148
Endoplasmic Reticulum 1 1 1 0 0 1 0 0 0 0 0 0 0 0 0 9 13 7 0 0 0 0 0 2
Endosome 0 0 0 0 0 0 2 0 1 0 1 6 0 2 1 0 2 0 1 2 2 0 2 1
Golgi 0 1 0 1 1 0 0 0 0 0 0 0 0 2 0 1 0 0 1 1 0 0 0 0
Mitochondria 20 5 0 1 5 13 55 21 78 51 124 58 0 0 0 1 3 5 4 5 16 3 8 7
Nucleus 0 0 0 1 1 0 0 0 4 0 3 5 0 1 0 3 2 1 0 0 0 1 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 1 3 2 2 2 0 6 5 1 6 0 0 0 0 0 0 3 1 2 0 3 4
Vac/Vac Membrane 1 1 0 2 0 2 3 0 0 1 0 2 0 0 1 0 4 5 2 3 1 0 2 3
Unique Cell Count 169 142 178 207 288 269 351 245 300 247 259 260 137 211 177 125 176 137 158 150 161 97 181 186
Labelled Cell Count 183 146 185 217 309 281 384 268 349 275 332 306 144 215 179 139 198 153 158 150 161 97 181 186


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 2.9 4.0 4.6 4.7 4.5 3.8 3.6 3.9 3.4 3.4 3.3 3.5 4.8 5.3 5.5 6.0 6.0 5.6 4.7 5.2 5.4
Std Deviation (1e-4) 0.6 1.2 1.7 2.5 1.8 2.1 2.1 1.4 1.8 1.2 2.2 1.4 1.4 2.3 2.7 1.5 1.7 1.4 2.0 2.6 2.0
Intensity Change (Log2) – – – 0.03 -0.02 -0.27 -0.33 -0.24 -0.43 -0.44 -0.46 -0.39 0.06 0.22 0.26 0.4 0.39 0.3 0.03 0.2 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.3237 -0.1709 -0.2662 -0.7881 -0.5379 -0.4715 0.5659 -0.4715 -0.4962 -0.4182 0.498 -0.5601 0.6782 -1.6145 -1.6543 -1.4966 -1.7712 -1.3614
Actin 0.0193 0.0044 0.0089 0.0002 0.0148 0.0016 0.0385 0.0019 0.0175 0.0091 0.0145 0.0069 0.1033 0.0006 0.0022 0.0244 0.0158 0.0223
Bud 0.0003 0.0001 0.0002 0.0024 0.0023 0.0003 0.0024 0.0008 0.0008 0.0003 0.0005 0.0006 0.0018 0.0026 0.0007 0.0027 0.0009 0.0008
Bud Neck 0.0007 0.0001 0.0026 0.0002 0.0015 0.0002 0.0154 0.0002 0.0018 0.0019 0.0445 0.0011 0.0487 0 0.0002 0.0038 0.0001 0.0009
Bud Periphery 0.0004 0.0001 0.0003 0.0041 0.008 0.0022 0.008 0.0009 0.0011 0.0006 0.001 0.0016 0.0028 0.0084 0.0011 0.0068 0.0029 0.0026
Bud Site 0.0006 0.0099 0.01 0.0014 0.0054 0 0.0104 0.0136 0.0041 0.0005 0.0049 0.0004 0.0357 0.0006 0.0012 0.0067 0.0006 0.0003
Cell Periphery 0.0001 0.0001 0.0002 0.0002 0.0003 0.0001 0.0007 0.0001 0.0001 0.0001 0.0002 0.0001 0.0021 0.0001 0.0001 0.0003 0.0002 0.0001
Cytoplasm 0.0441 0.0156 0.016 0.0246 0.0422 0.047 0.0258 0.0238 0.014 0.0102 0.0335 0.0304 0.0939 0.0441 0.0384 0.0678 0.0434 0.1152
Cytoplasmic Foci 0.0202 0.0016 0.0061 0.0012 0.018 0.0082 0.0382 0.0029 0.0116 0.0225 0.0117 0.0068 0.0362 0.0052 0.0082 0.0232 0.0031 0.022
Eisosomes 0.0012 0.0003 0.0003 0.0002 0.0001 0.0001 0.0006 0.0003 0.0003 0.0002 0.0001 0.0002 0.0005 0.0002 0.0002 0.0004 0.0007 0.0002
Endoplasmic Reticulum 0.0054 0.0001 0.0004 0.0002 0.0044 0.0002 0.0045 0.0005 0.0014 0.0003 0.0006 0.0011 0.0026 0.0005 0.0005 0.0006 0.0014 0.0009
Endosome 0.0213 0.0001 0.005 0.0001 0.0441 0.0005 0.0388 0.0008 0.0076 0.0074 0.0042 0.0056 0.0464 0.0007 0.0124 0.0093 0.0075 0.0157
Golgi 0.0044 0.0001 0.0022 0 0.0069 0.0003 0.0114 0.0001 0.0066 0.0056 0.0015 0.003 0.0137 0.0002 0.0138 0.0029 0.0045 0.012
Lipid Particles 0.0272 0.0001 0.0067 0.0001 0.0059 0.0012 0.0724 0.0004 0.0081 0.01 0.0029 0.005 0.0553 0.0009 0.0052 0.0026 0.0092 0.0292
Mitochondria 0.0023 0.0002 0.0091 0.0002 0.0068 0.0003 0.0221 0.0008 0.0121 0.0099 0.0004 0.0113 0.0547 0.0009 0.0073 0.0009 0.0578 0.01
None 0.792 0.9662 0.9211 0.963 0.79 0.9232 0.6085 0.9483 0.8813 0.8862 0.8113 0.9087 0.378 0.9317 0.9033 0.8207 0.8193 0.7474
Nuclear Periphery 0.0145 0 0.0012 0.0002 0.012 0.0003 0.0099 0.0003 0.0039 0.0013 0.0087 0.0027 0.0048 0.0002 0.0005 0.0003 0.0031 0.0009
Nucleolus 0.0008 0.0001 0.001 0.0003 0.0007 0.0001 0.006 0.0006 0.0016 0.0004 0.0015 0.0005 0.0242 0.0003 0.0002 0.0019 0.0004 0.0009
Nucleus 0.0163 0.0001 0.0009 0.0007 0.0038 0.0004 0.0086 0.0011 0.0035 0.0011 0.0111 0.0009 0.0102 0.0004 0.0005 0.0009 0.0011 0.0007
Peroxisomes 0.0072 0.0003 0.0045 0.0001 0.0076 0.0113 0.0282 0.0007 0.0122 0.025 0.0215 0.0075 0.0236 0.0004 0.0019 0.0121 0.0248 0.0112
Punctate Nuclear 0.0201 0.0002 0.0024 0.0004 0.0124 0.0025 0.0424 0.0016 0.0097 0.0069 0.025 0.0049 0.0414 0.0007 0.0008 0.0029 0.0007 0.0049
Vacuole 0.001 0.0001 0.0004 0.0003 0.0075 0.0001 0.0053 0.0003 0.0005 0.0003 0.0005 0.0005 0.0164 0.0012 0.0008 0.008 0.0011 0.0013
Vacuole Periphery 0.0007 0 0.0004 0 0.0051 0 0.002 0.0001 0.0003 0.0003 0.0002 0.0004 0.0037 0.0001 0.0003 0.0007 0.0014 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.9675 7.1283 10.579 11.5071 11.631 23.9088 10.5859 15.2016 15.3941 12.2412
Translational Efficiency 0.9523 1.2791 1.0524 0.9327 0.7186 0.5406 1.3972 0.8606 0.7789 0.9774

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
22 846 1862 1622 1320 1129 226 779 1342 1975 2088 2401

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1204.10 629.86 774.29 779.99 640.42 621.07 794.78 806.75 649.66 624.84 776.51 788.67
Standard Deviation 853.54 94.25 104.03 138.88 79.61 77.97 116.34 132.56 152.64 85.43 105.63 137.43
Intensity Change Log 2 — -0.934852 -0.637009 -0.626428 — -0.044262 0.311537 0.333103 — -0.560244 -0.233336 -0.217180

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001535 0.000287 0.000148 0.000665 0.000056 0.001573 0.000087 0.003417 0.000080 0.001022 0.000141 0.001558
Bud Neck 0.048897 0.038197 0.009866 0.014458 0.011281 0.032857 0.001928 0.011235 0.011898 0.035144 0.009006 0.013412
Bud Site 0.054484 0.016432 0.014709 0.057209 0.001941 0.015413 0.002141 0.088966 0.002803 0.015849 0.013348 0.067513
Cell Periphery 0.000528 0.000224 0.000263 0.000328 0.000152 0.000214 0.000475 0.000424 0.000158 0.000218 0.000286 0.000359
Cytoplasm 0.256786* 0.416697* 0.340999* 0.419240* 0.516427* 0.453843* 0.320380* 0.416728* 0.512171* 0.437931* 0.338767* 0.418425*
Cytoplasmic Foci 0.099071 0.221388* 0.011911 0.015320 0.144923 0.210347* 0.060393 0.038598 0.144171 0.215076* 0.017159 0.022872
Eisosomes 0.000097 0.000178 0.000036 0.000047 0.000149 0.000143 0.000320 0.000051 0.000148 0.000158 0.000067 0.000048
Endoplasmic Reticulum 0.007396 0.000507 0.001066 0.003021 0.000438 0.000457 0.000881 0.002404 0.000552 0.000478 0.001046 0.002821
Endosome 0.049698 0.022509 0.001802 0.006709 0.003631 0.015533 0.007065 0.030415 0.004386 0.018521 0.002372 0.014400
Golgi 0.011734 0.005209 0.000304 0.006790 0.000489 0.004825 0.004561 0.018127 0.000674 0.004990 0.000765 0.010468
Lipid Particles 0.006211 0.008868 0.000937 0.001102 0.004050 0.006971 0.002986 0.001440 0.004085 0.007784 0.001159 0.001211
Mitochondria 0.028988 0.013100 0.001875 0.006139 0.001640 0.011005 0.000463 0.009441 0.002088 0.011903 0.001722 0.007210
Mitotic Spindle 0.081344 0.001615 0.014595 0.049321 0.000316 0.005450 0.004920 0.061773 0.001644 0.003807 0.013548 0.053361
None 0.016524 0.011622 0.010800 0.007593 0.005666 0.005660 0.021681 0.013367 0.005844 0.008214 0.011977 0.009467
Nuclear Periphery 0.001317 0.000373 0.001361 0.001892 0.000501 0.000374 0.000746 0.000969 0.000514 0.000374 0.001295 0.001593
Nuclear Periphery Foci 0.031123 0.000379 0.000268 0.001807 0.000201 0.000469 0.000473 0.001014 0.000708 0.000431 0.000290 0.001550
Nucleolus 0.027496 0.003334 0.001271 0.000611 0.000624 0.001675 0.000973 0.000779 0.001064 0.002386 0.001239 0.000665
Nucleus 0.135780* 0.143346* 0.561434* 0.302073* 0.264680* 0.142871* 0.518314* 0.166040* 0.262567* 0.143074* 0.556767* 0.257938*
Peroxisomes 0.008562 0.017506 0.000681 0.000463 0.001931 0.020696 0.000393 0.004574 0.002039 0.019329 0.000650 0.001797
Vacuole 0.098653 0.075677 0.024581 0.095933 0.040526 0.068517 0.049478 0.116716 0.041479 0.071584 0.027276 0.102676
Vacuole Periphery 0.033775 0.002554 0.001092 0.009278 0.000379 0.001107 0.001343 0.013523 0.000927 0.001727 0.001119 0.010655

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.02 2.14 1.79 -3.22 -5.13 -1.83 -2.29 -3.77 -0.10 -3.52 -1.96 -3.26 -5.50 0.25 -4.94
Bud Neck 0.32 1.54 1.44 7.40 -1.79 -7.83 8.10 2.15 9.10 -6.88 -10.03 1.81 0.51 11.15 -1.72
Bud Site 1.62 1.74 0.95 -4.69 -5.84 -7.43 -0.98 -8.50 -5.51 -8.30 -9.12 -6.04 -12.96 -7.26 -8.31
Cell Periphery 2.52 2.42 2.54 0.06 0.13 -0.85 -5.37 -2.10 -0.15 3.58 -1.39 -2.61 -3.26 -0.13 0.77
Cytoplasm -1.85 -0.42 -0.80 6.68 -3.06 6.01 12.68 15.29 9.22 -1.74 8.26 23.08 20.35 11.99 -3.33
Cytoplasmic Foci -4.41 2.63 2.60 28.85 -0.92 -8.99 14.84 26.20 29.42 6.71 -11.59 29.78 30.02 42.25 0.43
Eisosomes -1.81 2.23 1.63 11.37 -7.49 0.44 -3.03 7.32 9.41 4.87 -0.83 6.93 8.10 15.11 0.73
Endoplasmic Reticulum 3.30 2.78 1.64 -17.92 -11.64 -0.27 -5.20 -12.14 -12.14 -4.34 0.96 -10.26 -19.71 -21.67 -12.87
Endosome 1.31 2.47 2.37 9.88 -3.61 -9.11 -1.42 -4.15 1.69 -1.81 -12.11 3.80 -2.23 9.61 -5.22
Golgi 1.53 2.74 2.16 2.00 -3.16 -4.48 -1.07 -3.32 -0.78 -0.84 -5.93 0.20 -4.08 1.10 -3.90
Lipid Particles -1.09 2.15 1.96 6.44 -4.63 -3.16 1.38 7.84 6.17 5.94 -4.87 9.05 8.53 8.96 -2.16
Mitochondria 0.58 1.09 1.02 4.75 -1.68 -4.80 1.89 -1.65 3.31 -2.91 -6.46 0.63 -1.35 5.73 -2.47
Mitotic Spindle 1.48 1.22 0.83 -9.66 -4.94 -3.80 -1.39 -8.26 -6.92 -5.73 -2.06 -5.65 -12.05 -11.25 -6.81
None 0.44 0.77 1.21 2.50 2.87 -0.01 -2.26 -1.30 -1.44 1.79 -1.56 -2.76 -0.65 1.33 2.87
Nuclear Periphery 1.62 -1.19 -3.87 -20.27 -10.02 1.35 -4.94 -12.92 -16.70 -7.48 1.50 -12.07 -19.05 -25.81 -10.97
Nuclear Periphery Foci 1.41 1.41 1.36 -5.99 -6.21 -1.69 -4.83 -9.78 -4.05 -3.82 0.77 0.91 -1.47 -6.13 -7.95
Nucleolus 1.99 2.18 2.22 3.90 1.98 -6.39 -2.44 -0.17 6.34 2.35 -3.49 -0.16 1.99 5.63 2.08
Nucleus -0.22 -10.38 -5.15 -22.80 22.67 15.35 -12.00 2.04 -9.35 12.38 16.61 -35.86 -7.51 -25.49 29.13
Peroxisomes -2.60 2.83 2.95 10.02 1.43 -7.36 5.05 0.21 7.26 -2.33 -10.51 4.09 3.53 11.42 -0.71
Vacuole 1.11 2.45 -2.43 -14.63 -23.64 -6.56 -4.57 -21.16 -18.00 -10.41 -8.03 0.03 -28.84 -23.20 -29.16
Vacuole Periphery 1.76 1.86 1.69 -1.28 -3.34 -4.96 -1.12 -3.16 -2.64 -2.42 -2.45 0.00 -4.14 -3.21 -4.20
Standard name
Human Ortholog
Description Cyclin-dependent kinase-activating kinase; required for passage through the cell cycle; phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases
Localization
Cell Percentages cytoplasm (35%), nucleus (5%), mixed (49%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cak1

Cak1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cak1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available