Standard name | |
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Human Ortholog | |
Description | Cyclin-dependent kinase-activating kinase; required for passage through the cell cycle; phosphorylates and activates Cdc28p; nucleotide-binding pocket differs significantly from those of most other protein kinases |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 3 | 1 | 3 | 3 |
Bud | 0 | 1 | 0 | 0 | 1 | 0 | 5 | 2 | 5 | 0 | 7 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 3 | 2 |
Bud Neck | 2 | 1 | 5 | 4 | 13 | 7 | 3 | 17 | 5 | 7 | 2 | 11 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 3 | 3 | 1 | 0 | 0 | 1 | 1 | 2 | 1 | 5 | 6 | 0 | 1 | 3 | 4 | 4 | 0 | 0 | 3 | 0 | 1 | 1 |
Cytoplasm | 158 | 135 | 175 | 202 | 285 | 256 | 313 | 227 | 249 | 209 | 192 | 206 | 137 | 206 | 175 | 122 | 170 | 130 | 131 | 128 | 118 | 85 | 144 | 148 |
Endoplasmic Reticulum | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9 | 13 | 7 | 0 | 0 | 0 | 0 | 0 | 2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 6 | 0 | 2 | 1 | 0 | 2 | 0 | 1 | 2 | 2 | 0 | 2 | 1 |
Golgi | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 20 | 5 | 0 | 1 | 5 | 13 | 55 | 21 | 78 | 51 | 124 | 58 | 0 | 0 | 0 | 1 | 3 | 5 | 4 | 5 | 16 | 3 | 8 | 7 |
Nucleus | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 4 | 0 | 3 | 5 | 0 | 1 | 0 | 3 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 1 | 3 | 2 | 2 | 2 | 0 | 6 | 5 | 1 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 0 | 3 | 4 |
Vac/Vac Membrane | 1 | 1 | 0 | 2 | 0 | 2 | 3 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 4 | 5 | 2 | 3 | 1 | 0 | 2 | 3 |
Unique Cell Count | 169 | 142 | 178 | 207 | 288 | 269 | 351 | 245 | 300 | 247 | 259 | 260 | 137 | 211 | 177 | 125 | 176 | 137 | 158 | 150 | 161 | 97 | 181 | 186 |
Labelled Cell Count | 183 | 146 | 185 | 217 | 309 | 281 | 384 | 268 | 349 | 275 | 332 | 306 | 144 | 215 | 179 | 139 | 198 | 153 | 158 | 150 | 161 | 97 | 181 | 186 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 2.9 | 4.0 | 4.6 | 4.7 | 4.5 | 3.8 | 3.6 | 3.9 | 3.4 | 3.4 | 3.3 | 3.5 | 4.8 | 5.3 | 5.5 | 6.0 | 6.0 | 5.6 | 4.7 | 5.2 | 5.4 |
Std Deviation (1e-4) | 0.6 | 1.2 | 1.7 | 2.5 | 1.8 | 2.1 | 2.1 | 1.4 | 1.8 | 1.2 | 2.2 | 1.4 | 1.4 | 2.3 | 2.7 | 1.5 | 1.7 | 1.4 | 2.0 | 2.6 | 2.0 |
Intensity Change (Log2) | – | – | – | 0.03 | -0.02 | -0.27 | -0.33 | -0.24 | -0.43 | -0.44 | -0.46 | -0.39 | 0.06 | 0.22 | 0.26 | 0.4 | 0.39 | 0.3 | 0.03 | 0.2 | 0.26 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.3237 | -0.1709 | -0.2662 | -0.7881 | -0.5379 | -0.4715 | 0.5659 | -0.4715 | -0.4962 | -0.4182 | 0.498 | -0.5601 | 0.6782 | -1.6145 | -1.6543 | -1.4966 | -1.7712 | -1.3614 |
Actin | 0.0193 | 0.0044 | 0.0089 | 0.0002 | 0.0148 | 0.0016 | 0.0385 | 0.0019 | 0.0175 | 0.0091 | 0.0145 | 0.0069 | 0.1033 | 0.0006 | 0.0022 | 0.0244 | 0.0158 | 0.0223 |
Bud | 0.0003 | 0.0001 | 0.0002 | 0.0024 | 0.0023 | 0.0003 | 0.0024 | 0.0008 | 0.0008 | 0.0003 | 0.0005 | 0.0006 | 0.0018 | 0.0026 | 0.0007 | 0.0027 | 0.0009 | 0.0008 |
Bud Neck | 0.0007 | 0.0001 | 0.0026 | 0.0002 | 0.0015 | 0.0002 | 0.0154 | 0.0002 | 0.0018 | 0.0019 | 0.0445 | 0.0011 | 0.0487 | 0 | 0.0002 | 0.0038 | 0.0001 | 0.0009 |
Bud Periphery | 0.0004 | 0.0001 | 0.0003 | 0.0041 | 0.008 | 0.0022 | 0.008 | 0.0009 | 0.0011 | 0.0006 | 0.001 | 0.0016 | 0.0028 | 0.0084 | 0.0011 | 0.0068 | 0.0029 | 0.0026 |
Bud Site | 0.0006 | 0.0099 | 0.01 | 0.0014 | 0.0054 | 0 | 0.0104 | 0.0136 | 0.0041 | 0.0005 | 0.0049 | 0.0004 | 0.0357 | 0.0006 | 0.0012 | 0.0067 | 0.0006 | 0.0003 |
Cell Periphery | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0021 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0001 |
Cytoplasm | 0.0441 | 0.0156 | 0.016 | 0.0246 | 0.0422 | 0.047 | 0.0258 | 0.0238 | 0.014 | 0.0102 | 0.0335 | 0.0304 | 0.0939 | 0.0441 | 0.0384 | 0.0678 | 0.0434 | 0.1152 |
Cytoplasmic Foci | 0.0202 | 0.0016 | 0.0061 | 0.0012 | 0.018 | 0.0082 | 0.0382 | 0.0029 | 0.0116 | 0.0225 | 0.0117 | 0.0068 | 0.0362 | 0.0052 | 0.0082 | 0.0232 | 0.0031 | 0.022 |
Eisosomes | 0.0012 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0006 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0004 | 0.0007 | 0.0002 |
Endoplasmic Reticulum | 0.0054 | 0.0001 | 0.0004 | 0.0002 | 0.0044 | 0.0002 | 0.0045 | 0.0005 | 0.0014 | 0.0003 | 0.0006 | 0.0011 | 0.0026 | 0.0005 | 0.0005 | 0.0006 | 0.0014 | 0.0009 |
Endosome | 0.0213 | 0.0001 | 0.005 | 0.0001 | 0.0441 | 0.0005 | 0.0388 | 0.0008 | 0.0076 | 0.0074 | 0.0042 | 0.0056 | 0.0464 | 0.0007 | 0.0124 | 0.0093 | 0.0075 | 0.0157 |
Golgi | 0.0044 | 0.0001 | 0.0022 | 0 | 0.0069 | 0.0003 | 0.0114 | 0.0001 | 0.0066 | 0.0056 | 0.0015 | 0.003 | 0.0137 | 0.0002 | 0.0138 | 0.0029 | 0.0045 | 0.012 |
Lipid Particles | 0.0272 | 0.0001 | 0.0067 | 0.0001 | 0.0059 | 0.0012 | 0.0724 | 0.0004 | 0.0081 | 0.01 | 0.0029 | 0.005 | 0.0553 | 0.0009 | 0.0052 | 0.0026 | 0.0092 | 0.0292 |
Mitochondria | 0.0023 | 0.0002 | 0.0091 | 0.0002 | 0.0068 | 0.0003 | 0.0221 | 0.0008 | 0.0121 | 0.0099 | 0.0004 | 0.0113 | 0.0547 | 0.0009 | 0.0073 | 0.0009 | 0.0578 | 0.01 |
None | 0.792 | 0.9662 | 0.9211 | 0.963 | 0.79 | 0.9232 | 0.6085 | 0.9483 | 0.8813 | 0.8862 | 0.8113 | 0.9087 | 0.378 | 0.9317 | 0.9033 | 0.8207 | 0.8193 | 0.7474 |
Nuclear Periphery | 0.0145 | 0 | 0.0012 | 0.0002 | 0.012 | 0.0003 | 0.0099 | 0.0003 | 0.0039 | 0.0013 | 0.0087 | 0.0027 | 0.0048 | 0.0002 | 0.0005 | 0.0003 | 0.0031 | 0.0009 |
Nucleolus | 0.0008 | 0.0001 | 0.001 | 0.0003 | 0.0007 | 0.0001 | 0.006 | 0.0006 | 0.0016 | 0.0004 | 0.0015 | 0.0005 | 0.0242 | 0.0003 | 0.0002 | 0.0019 | 0.0004 | 0.0009 |
Nucleus | 0.0163 | 0.0001 | 0.0009 | 0.0007 | 0.0038 | 0.0004 | 0.0086 | 0.0011 | 0.0035 | 0.0011 | 0.0111 | 0.0009 | 0.0102 | 0.0004 | 0.0005 | 0.0009 | 0.0011 | 0.0007 |
Peroxisomes | 0.0072 | 0.0003 | 0.0045 | 0.0001 | 0.0076 | 0.0113 | 0.0282 | 0.0007 | 0.0122 | 0.025 | 0.0215 | 0.0075 | 0.0236 | 0.0004 | 0.0019 | 0.0121 | 0.0248 | 0.0112 |
Punctate Nuclear | 0.0201 | 0.0002 | 0.0024 | 0.0004 | 0.0124 | 0.0025 | 0.0424 | 0.0016 | 0.0097 | 0.0069 | 0.025 | 0.0049 | 0.0414 | 0.0007 | 0.0008 | 0.0029 | 0.0007 | 0.0049 |
Vacuole | 0.001 | 0.0001 | 0.0004 | 0.0003 | 0.0075 | 0.0001 | 0.0053 | 0.0003 | 0.0005 | 0.0003 | 0.0005 | 0.0005 | 0.0164 | 0.0012 | 0.0008 | 0.008 | 0.0011 | 0.0013 |
Vacuole Periphery | 0.0007 | 0 | 0.0004 | 0 | 0.0051 | 0 | 0.002 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0037 | 0.0001 | 0.0003 | 0.0007 | 0.0014 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.9675 | 7.1283 | 10.579 | 11.5071 | 11.631 | 23.9088 | 10.5859 | 15.2016 | 15.3941 | 12.2412 |
Translational Efficiency | 0.9523 | 1.2791 | 1.0524 | 0.9327 | 0.7186 | 0.5406 | 1.3972 | 0.8606 | 0.7789 | 0.9774 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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