Standard name | |
---|---|
Human Ortholog | |
Description | Activator of Cbk1p kinase; component of the RAM signaling network that regulates cellular polarity and morphogenesis; activation of Cbk1p facilitates the Ace2p-dependent daughter cell-specific transcription of genes involved in cell separation; similar to Mob1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 29 | – | 1 | 27 | 16 | 13 | 6 | 9 | 2 | 5 | 1 | 1 | 2 | 3 | 4 | 28 | 39 | 57 | 28 | 6 | 3 | 25 | 46 | 53 |
Bud | 16 | – | 0 | 7 | 5 | 5 | 11 | 15 | 11 | 6 | 6 | 3 | 1 | 4 | 2 | 9 | 26 | 28 | 16 | 2 | 3 | 31 | 33 | 39 |
Bud Neck | 1 | – | 0 | 9 | 17 | 11 | 9 | 18 | 11 | 2 | 1 | 1 | 0 | 3 | 1 | 0 | 9 | 1 | 33 | 5 | 12 | 65 | 73 | 41 |
Bud Site | 13 | – | 0 | 3 | 9 | 3 | 1 | 0 | 0 | 3 | 1 | 1 | 1 | 1 | 0 | 3 | 5 | 7 | – | – | – | – | – | – |
Cell Periphery | 19 | – | 1 | 25 | 23 | 4 | 16 | 12 | 8 | 3 | 3 | 8 | 4 | 57 | 55 | 43 | 100 | 94 | 26 | 1 | 3 | 6 | 7 | 8 |
Cytoplasm | 135 | – | 16 | 230 | 422 | 287 | 548 | 556 | 451 | 374 | 165 | 249 | 31 | 284 | 334 | 178 | 209 | 193 | 75 | 13 | 22 | 24 | 66 | 124 |
Endoplasmic Reticulum | 3 | – | 0 | 4 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 13 | 14 | 10 | 8 | 0 | 0 | 2 | 4 | 2 |
Endosome | 2 | – | 0 | 1 | 1 | 5 | 4 | 1 | 9 | 1 | 1 | 2 | 0 | 0 | 0 | 3 | 6 | 4 | 4 | 0 | 0 | 3 | 1 | 3 |
Golgi | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 6 | – | 0 | 0 | 1 | 1 | 5 | 8 | 9 | 5 | 4 | 2 | 0 | 0 | 0 | 2 | 4 | 1 | 5 | 0 | 2 | 2 | 1 | 2 |
Nucleus | 0 | – | 0 | 3 | 5 | 4 | 7 | 3 | 3 | 2 | 0 | 2 | 0 | 1 | 0 | 1 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | – | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | – | 0 | 1 | 2 | 1 | 2 | 3 | 9 | 1 | 1 | 2 | 0 | 0 | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 6 | – | 0 | 2 | 2 | 2 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 9 | – | 0 | 22 | 55 | 56 | 29 | 23 | 26 | 11 | 7 | 6 | 4 | 2 | 0 | 10 | 27 | 14 | 0 | 0 | 2 | 2 | 2 | 1 |
Vac/Vac Membrane | 0 | – | 0 | 6 | 6 | 3 | 24 | 5 | 22 | 5 | 15 | 14 | 0 | 0 | 0 | 6 | 24 | 15 | 9 | 0 | 0 | 2 | 1 | 1 |
Unique Cell Count | 182 | 17 | 290 | 506 | 355 | 609 | 617 | 518 | 400 | 196 | 269 | 37 | 290 | 339 | 234 | 337 | 312 | 209 | 33 | 53 | 170 | 245 | 286 | |
Labelled Cell Count | 239 | 18 | 341 | 565 | 395 | 663 | 655 | 562 | 419 | 206 | 291 | 43 | 356 | 399 | 297 | 477 | 427 | 209 | 33 | 53 | 170 | 245 | 286 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.8 | – | 5.9 | 7.6 | 6.9 | 6.2 | 6.0 | 5.8 | 5.6 | 5.5 | 6.9 | 6.0 | 7.3 | 7.1 | 6.8 | 9.4 | 10.3 | 11.2 | 6.6 | 7.2 | 7.4 |
Std Deviation (1e-4) | 1.0 | – | 0.6 | 7.2 | 1.8 | 7.4 | 5.5 | 1.2 | 1.2 | 1.0 | 4.2 | 1.2 | 1.2 | 1.5 | 1.2 | 1.8 | 2.0 | 2.0 | 1.3 | 1.2 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.38 | 0.24 | 0.09 | 0.03 | -0.02 | -0.06 | -0.08 | 0.23 | 0.04 | 0.32 | 0.28 | 0.22 | 0.69 | 0.82 | 0.94 | 0.17 | 0.29 | 0.33 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.5 | -1.2 | -1.4 | -0.6 | -0.5 | -0.9 | -0.1 | -1.1 | -0.2 | -1.0 | 1.0 | 1.4 | -1.7 | -2.7 | -2.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.805 | 2.4079 | 2.085 | 1.6769 | 1.7861 | 2.3802 | 1.6409 | 2.5185 | 2.3757 | 1.8601 | 2.0315 | 2.2766 | 1.4677 | 2.3262 | 1.819 | 2.3185 | 1.1043 | 1.8283 |
Actin | 0.0156 | 0.0088 | 0.031 | 0.0118 | 0.0482 | 0.0198 | 0.0323 | 0.007 | 0.0212 | 0.027 | 0.046 | 0.0132 | 0.0186 | 0.0104 | 0.0369 | 0.1526 | 0.1203 | 0.0243 |
Bud | 0.0049 | 0.004 | 0.0329 | 0.0139 | 0.0115 | 0.0062 | 0.0014 | 0.0017 | 0.0186 | 0.0065 | 0.0098 | 0.0027 | 0.0044 | 0.0014 | 0.0184 | 0.0433 | 0.0313 | 0.0016 |
Bud Neck | 0.0235 | 0.0021 | 0.0107 | 0.0235 | 0.0049 | 0.4128 | 0.0203 | 0.006 | 0.0056 | 0.0022 | 0.0018 | 0.6708 | 0.02 | 0.0026 | 0.0103 | 0.0026 | 0.0759 | 0.6659 |
Bud Periphery | 0.0291 | 0.0068 | 0.0837 | 0.1198 | 0.0592 | 0.0122 | 0.013 | 0.004 | 0.0793 | 0.0832 | 0.1784 | 0.0263 | 0.021 | 0.0017 | 0.0683 | 0.1979 | 0.1312 | 0.0084 |
Bud Site | 0.0787 | 0.1913 | 0.0615 | 0.0324 | 0.0069 | 0.007 | 0.0455 | 0.1892 | 0.0282 | 0.008 | 0.002 | 0.0013 | 0.0601 | 0.108 | 0.039 | 0.0072 | 0.0131 | 0.0053 |
Cell Periphery | 0.0407 | 0.0189 | 0.0225 | 0.0437 | 0.0485 | 0.0081 | 0.0509 | 0.0283 | 0.0298 | 0.0666 | 0.1335 | 0.0211 | 0.032 | 0.0196 | 0.0242 | 0.0213 | 0.0196 | 0.0083 |
Cytoplasm | 0.5892 | 0.6313 | 0.6126 | 0.6289 | 0.5556 | 0.4103 | 0.6236 | 0.6394 | 0.7485 | 0.5502 | 0.2547 | 0.2416 | 0.6972 | 0.7531 | 0.7006 | 0.4924 | 0.4978 | 0.2456 |
Cytoplasmic Foci | 0.0119 | 0.0088 | 0.0047 | 0.0038 | 0.008 | 0.0135 | 0.0114 | 0.0016 | 0.0023 | 0.0163 | 0.0136 | 0.0015 | 0.0214 | 0.0029 | 0.0107 | 0.0108 | 0.0155 | 0.0033 |
Eisosomes | 0.0012 | 0.0004 | 0.0004 | 0.0014 | 0.001 | 0.0003 | 0.0006 | 0.0002 | 0.0003 | 0.0004 | 0.0008 | 0.0001 | 0.0007 | 0.0003 | 0.0005 | 0.0007 | 0.0004 | 0.0001 |
Endoplasmic Reticulum | 0.008 | 0.0055 | 0.0036 | 0.006 | 0.0072 | 0.0027 | 0.0164 | 0.0032 | 0.0073 | 0.0206 | 0.0037 | 0.001 | 0.0071 | 0.0028 | 0.0065 | 0.0066 | 0.0034 | 0.0034 |
Endosome | 0.009 | 0.0024 | 0.001 | 0.001 | 0.0114 | 0.0029 | 0.0082 | 0.0002 | 0.001 | 0.0293 | 0.0237 | 0.0052 | 0.0072 | 0.0003 | 0.0021 | 0.0055 | 0.0074 | 0.0006 |
Golgi | 0.0026 | 0.001 | 0.001 | 0.0007 | 0.0087 | 0.0008 | 0.0052 | 0.0002 | 0.0013 | 0.0266 | 0.0184 | 0.004 | 0.0019 | 0.0003 | 0.0025 | 0.0102 | 0.0086 | 0.0004 |
Lipid Particles | 0.0079 | 0.0008 | 0.0006 | 0.0005 | 0.003 | 0.0008 | 0.0118 | 0.0006 | 0.0022 | 0.0255 | 0.0284 | 0.0011 | 0.0045 | 0.0006 | 0.0042 | 0.0157 | 0.0154 | 0.0009 |
Mitochondria | 0.0009 | 0.0005 | 0.0016 | 0.0009 | 0.003 | 0.0006 | 0.016 | 0 | 0.0024 | 0.044 | 0.0763 | 0.001 | 0.0021 | 0.0001 | 0.0009 | 0.0028 | 0.0022 | 0.0001 |
None | 0.1573 | 0.1096 | 0.1233 | 0.1033 | 0.2131 | 0.083 | 0.112 | 0.1159 | 0.0463 | 0.0607 | 0.0132 | 0.0068 | 0.0745 | 0.093 | 0.0602 | 0.007 | 0.0277 | 0.0246 |
Nuclear Periphery | 0.0023 | 0.0006 | 0.0006 | 0.0008 | 0.0016 | 0.0034 | 0.0059 | 0.0003 | 0.0005 | 0.0036 | 0.0271 | 0.0005 | 0.0055 | 0.0003 | 0.0009 | 0.0018 | 0.0051 | 0.0001 |
Nucleolus | 0.0017 | 0.0015 | 0.0016 | 0.0003 | 0.0003 | 0.0017 | 0.0004 | 0.0001 | 0.0002 | 0.0008 | 0.0041 | 0.0001 | 0.0016 | 0.0003 | 0.0003 | 0.0001 | 0.0006 | 0.0024 |
Nucleus | 0.0074 | 0.0018 | 0.0028 | 0.0014 | 0.0017 | 0.005 | 0.0019 | 0.0006 | 0.0005 | 0.0017 | 0.0119 | 0.0005 | 0.0037 | 0.0006 | 0.0012 | 0.0008 | 0.0013 | 0.0023 |
Peroxisomes | 0.0014 | 0.0002 | 0.0003 | 0.0006 | 0.001 | 0.0005 | 0.0179 | 0 | 0.0025 | 0.0162 | 0.0976 | 0.0004 | 0.0036 | 0 | 0.0074 | 0.0139 | 0.0068 | 0.0002 |
Punctate Nuclear | 0.0022 | 0.0018 | 0.0015 | 0.0006 | 0.001 | 0.0062 | 0.0011 | 0.0002 | 0.0002 | 0.001 | 0.0521 | 0.0005 | 0.0098 | 0.0003 | 0.003 | 0.0041 | 0.0136 | 0.0005 |
Vacuole | 0.0043 | 0.0017 | 0.0021 | 0.0045 | 0.0035 | 0.0018 | 0.0032 | 0.0012 | 0.0016 | 0.0061 | 0.0018 | 0.0004 | 0.0026 | 0.0014 | 0.0019 | 0.0024 | 0.0022 | 0.0017 |
Vacuole Periphery | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0.0007 | 0.0003 | 0.0009 | 0 | 0.0001 | 0.0032 | 0.0012 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0003 | 0.0004 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 24.2647 | 13.6997 | 8.4715 | 23.0044 | 24.0091 | 21.6985 | 17.3147 | 15.9712 | 16.0636 | 22.8811 |
Translational Efficiency | 1.8319 | 2.2542 | 2.6991 | 0.9192 | 1.1284 | 2.1621 | 1.5402 | 1.5866 | 1.6081 | 1.5495 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1706 | 1047 | 2100 | 1462 | 150 | 1176 | 1146 | 648 | 1856 | 2223 | 3246 | 2110 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 788.68 | 874.31 | 989.44 | 956.17 | 795.16 | 892.92 | 943.20 | 1051.77 | 789.20 | 884.15 | 973.11 | 985.53 |
Standard Deviation | 85.37 | 104.95 | 108.45 | 156.40 | 95.11 | 115.05 | 131.33 | 161.03 | 86.22 | 110.80 | 119.11 | 163.88 |
Intensity Change Log 2 | — | 0.148705 | 0.327172 | 0.277827 | — | 0.167286 | 0.246319 | 0.403502 | — | 0.158063 | 0.287146 | 0.342290 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.003767 | 0.012131 | 0.009543 | 0.022125 | 0.003726 | 0.009434 | 0.010948 | 0.046575 | 0.003763 | 0.010705 | 0.010039 | 0.029634 |
Bud Neck | 0.136151 | 0.132517 | 0.137306 | 0.073024 | 0.118529 | 0.198191* | 0.093498 | 0.043569 | 0.134727 | 0.167260 | 0.121840 | 0.063978 |
Bud Site | 0.116636* | 0.256674* | 0.292725* | 0.348773* | 0.087551 | 0.283410* | 0.269235* | 0.328498* | 0.114286* | 0.270818* | 0.284432* | 0.342546* |
Cell Periphery | 0.002245 | 0.005142 | 0.005724 | 0.003862 | 0.002484 | 0.006247 | 0.003262 | 0.009058 | 0.002265 | 0.005726 | 0.004854 | 0.005458 |
Cytoplasm | 0.466444* | 0.351937* | 0.353492* | 0.370845* | 0.369168* | 0.343452* | 0.347958* | 0.353790* | 0.458583* | 0.347448* | 0.351538* | 0.365607* |
Cytoplasmic Foci | 0.075335 | 0.097698 | 0.014929 | 0.017870 | 0.163097 | 0.042891 | 0.039567 | 0.027948 | 0.082428 | 0.068704 | 0.023628 | 0.020965 |
Eisosomes | 0.002075 | 0.002085 | 0.000779 | 0.000647 | 0.002423 | 0.002870 | 0.000730 | 0.002103 | 0.002103 | 0.002501 | 0.000762 | 0.001094 |
Endoplasmic Reticulum | 0.001544 | 0.005148 | 0.004696 | 0.001125 | 0.003056 | 0.002323 | 0.003167 | 0.002279 | 0.001666 | 0.003654 | 0.004156 | 0.001479 |
Endosome | 0.008805 | 0.012599 | 0.001535 | 0.004559 | 0.018968 | 0.007746 | 0.004459 | 0.003439 | 0.009626 | 0.010032 | 0.002567 | 0.004215 |
Golgi | 0.003264 | 0.005094 | 0.000330 | 0.002983 | 0.003942 | 0.004043 | 0.000600 | 0.003324 | 0.003319 | 0.004538 | 0.000426 | 0.003088 |
Lipid Particles | 0.004482 | 0.004581 | 0.001047 | 0.001038 | 0.006000 | 0.002413 | 0.001841 | 0.002060 | 0.004605 | 0.003434 | 0.001327 | 0.001352 |
Mitochondria | 0.001669 | 0.001587 | 0.000818 | 0.002497 | 0.002944 | 0.002296 | 0.000698 | 0.001102 | 0.001772 | 0.001962 | 0.000776 | 0.002068 |
Mitotic Spindle | 0.000110 | 0.000455 | 0.002130 | 0.003117 | 0.000444 | 0.003844 | 0.002753 | 0.007154 | 0.000137 | 0.002248 | 0.002350 | 0.004357 |
None | 0.001211 | 0.000499 | 0.000382 | 0.000454 | 0.000144 | 0.000403 | 0.000618 | 0.000264 | 0.001125 | 0.000448 | 0.000465 | 0.000396 |
Nuclear Periphery | 0.000081 | 0.000048 | 0.000126 | 0.000123 | 0.000091 | 0.000049 | 0.000573 | 0.000098 | 0.000082 | 0.000049 | 0.000284 | 0.000115 |
Nuclear Periphery Foci | 0.000509 | 0.000858 | 0.001017 | 0.000901 | 0.001050 | 0.000421 | 0.002610 | 0.001970 | 0.000553 | 0.000627 | 0.001580 | 0.001229 |
Nucleolus | 0.000405 | 0.000132 | 0.000084 | 0.000152 | 0.000156 | 0.000155 | 0.000193 | 0.000059 | 0.000385 | 0.000144 | 0.000123 | 0.000124 |
Nucleus | 0.060321 | 0.021370 | 0.064315 | 0.020248 | 0.035612 | 0.022989 | 0.045537 | 0.019109 | 0.058324 | 0.022227 | 0.057686 | 0.019898 |
Peroxisomes | 0.003980 | 0.006396 | 0.001309 | 0.002161 | 0.008914 | 0.003983 | 0.002135 | 0.003653 | 0.004379 | 0.005119 | 0.001601 | 0.002619 |
Vacuole | 0.110378 | 0.082200 | 0.107605 | 0.121906 | 0.170700 | 0.061650 | 0.169191 | 0.142732 | 0.115253 | 0.071329 | 0.129348 | 0.128302 |
Vacuole Periphery | 0.000587 | 0.000849 | 0.000106 | 0.001592 | 0.001001 | 0.001188 | 0.000429 | 0.001215 | 0.000620 | 0.001028 | 0.000220 | 0.001476 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -6.90 | -6.70 | -8.96 | -3.36 | -5.59 | -5.23 | -4.96 | -8.24 | -6.86 | -6.72 | -8.99 | -8.36 | -12.03 | -7.65 | -8.65 |
Bud Neck | 0.35 | 2.68 | 11.08 | 9.22 | 9.86 | -4.11 | 2.04 | 4.41 | 18.19 | 6.97 | -4.16 | 4.80 | 13.13 | 18.81 | 11.62 |
Bud Site | -14.51 | -17.66 | -18.21 | -2.42 | -3.23 | -13.10 | -9.38 | -10.61 | 1.57 | -2.54 | -21.50 | -19.76 | -21.08 | -0.78 | -4.21 |
Cell Periphery | -5.81 | -4.55 | -2.42 | 3.57 | 2.78 | -5.08 | -0.58 | -4.01 | -0.67 | -4.07 | -8.74 | -4.57 | -4.88 | 2.70 | -0.30 |
Cytoplasm | 10.51 | 10.83 | 7.60 | -3.02 | -2.16 | 1.09 | 0.66 | 0.43 | -1.05 | -0.35 | 12.52 | 11.85 | 8.84 | -3.48 | -2.11 |
Cytoplasmic Foci | -4.31 | 20.23 | 19.04 | 17.39 | -1.65 | 9.15 | 9.40 | 10.24 | 4.33 | 3.91 | 3.63 | 19.37 | 20.29 | 17.36 | 2.41 |
Eisosomes | -0.06 | 7.11 | 7.79 | 6.81 | 1.62 | -0.87 | 3.54 | 1.29 | 2.96 | -3.85 | -1.58 | 7.89 | 6.12 | 7.39 | -2.14 |
Endoplasmic Reticulum | -5.05 | -7.12 | 1.29 | 5.82 | 8.56 | 0.76 | -0.50 | 0.67 | -0.26 | 2.27 | -4.38 | -7.41 | 0.31 | 5.11 | 8.89 |
Endosome | -3.31 | 11.47 | 4.83 | 6.85 | -2.52 | 3.38 | 4.54 | 4.93 | 5.03 | 1.76 | -0.53 | 10.86 | 6.98 | 7.58 | -1.47 |
Golgi | -3.21 | 10.43 | 1.10 | 2.83 | -2.28 | -0.15 | 3.00 | 0.98 | 1.36 | -1.76 | -2.38 | 10.55 | 1.40 | 2.78 | -2.83 |
Lipid Particles | -0.10 | 7.95 | 8.08 | 5.73 | 1.07 | 3.94 | 4.42 | 3.46 | -0.68 | -1.66 | 2.45 | 8.04 | 7.53 | 5.46 | -0.43 |
Mitochondria | 0.21 | 2.86 | -0.31 | -0.39 | -1.48 | 0.29 | 1.21 | 1.08 | 2.77 | -0.45 | -0.58 | 3.60 | 0.34 | 0.67 | -1.56 |
Mitotic Spindle | -3.90 | -4.01 | -3.65 | -3.13 | -0.66 | -2.18 | -2.02 | -2.30 | -0.54 | -1.21 | -2.58 | -4.42 | -4.14 | -1.05 | -1.32 |
None | 2.22 | 3.15 | 2.73 | 0.29 | 0.00 | -3.23 | -3.31 | -1.05 | 1.18 | 2.18 | 2.72 | 2.71 | 2.97 | 0.47 | 1.00 |
Nuclear Periphery | 2.16 | -5.84 | -1.49 | -2.55 | 1.33 | 3.67 | -1.36 | -1.10 | -2.27 | 1.22 | 2.29 | -1.99 | -1.84 | -3.24 | 1.46 |
Nuclear Periphery Foci | -4.05 | -8.15 | -2.78 | -0.65 | 1.42 | 3.39 | -6.19 | -2.51 | -3.76 | 0.96 | -1.28 | -11.27 | -4.30 | -3.90 | 1.92 |
Nucleolus | 6.79 | 7.84 | 4.37 | -0.00 | -0.83 | 0.74 | 0.12 | 3.36 | 4.17 | 3.12 | 6.51 | 6.92 | 5.58 | 1.00 | 0.33 |
Nucleus | 14.86 | -5.25 | 12.39 | -2.12 | 16.53 | 3.33 | -3.86 | 1.76 | -1.95 | 7.14 | 15.62 | -4.05 | 12.71 | -2.64 | 17.85 |
Peroxisomes | -3.03 | 6.72 | 4.94 | 6.57 | -2.30 | 1.84 | 2.65 | 2.21 | 1.34 | -1.58 | -1.14 | 6.76 | 4.70 | 6.26 | -2.60 |
Vacuole | 5.66 | -6.19 | -10.56 | -13.75 | -5.63 | 7.04 | -2.43 | -2.50 | -15.50 | -0.39 | 11.27 | -10.78 | -12.91 | -21.16 | -4.33 |
Vacuole Periphery | -2.21 | 7.66 | -1.71 | -0.99 | -3.06 | -0.34 | 1.40 | 0.15 | 0.43 | -0.81 | -1.62 | 5.04 | -1.54 | -0.21 | -2.88 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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