Standard name | |
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Human Ortholog | |
Description | Positive regulator of the Gcn2p kinase activity; forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 10 | 13 | 9 | 10 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 5 | 6 | 7 | 15 | 13 | 16 | 10 | 13 | 7 | 23 | 7 | 8 | 6 | 4 | 8 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 81 | 160 | 177 | 237 | 267 | 297 | 241 | 272 | 253 | 217 | 139 | 159 | 216 | 314 | 360 | 103 | 106 | 81 | 77 | 159 | 182 | 115 | 269 | 250 |
Endoplasmic Reticulum | 3 | 3 | 13 | 6 | 24 | 35 | 27 | 69 | 48 | 32 | 17 | 26 | 15 | 12 | 9 | 3 | 7 | 1 | 1 | 0 | 4 | 15 | 31 | 27 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 8 | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Mitochondria | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 10 | 21 | 31 | 42 | 21 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 4 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 0 | 1 | 2 | 1 | 2 | 21 | 0 | 7 | 4 | 9 | 1 | 2 | 0 | 4 | 1 | 2 | 0 | 1 | 5 | 5 | 7 | 8 |
Unique Cell Count | 85 | 163 | 187 | 242 | 294 | 336 | 275 | 368 | 321 | 287 | 194 | 223 | 234 | 330 | 372 | 108 | 112 | 85 | 86 | 163 | 194 | 146 | 315 | 295 |
Labelled Cell Count | 85 | 167 | 195 | 250 | 300 | 355 | 290 | 407 | 348 | 314 | 223 | 259 | 239 | 336 | 376 | 115 | 123 | 88 | 86 | 163 | 194 | 146 | 315 | 295 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 28.3 | 31.8 | 24.0 | 22.3 | 21.2 | 18.1 | 16.3 | 15.3 | 14.5 | 12.9 | 12.3 | 13.1 | 30.9 | 31.4 | 31.2 | 33.1 | 35.2 | 33.0 | 27.0 | 28.0 | 30.1 |
Std Deviation (1e-4) | 6.9 | 5.6 | 4.3 | 3.7 | 3.3 | 2.8 | 2.9 | 3.0 | 2.6 | 2.4 | 2.4 | 2.6 | 6.5 | 5.8 | 5.4 | 7.4 | 9.0 | 7.5 | 6.0 | 5.9 | 7.9 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.18 | -0.41 | -0.56 | -0.65 | -0.73 | -0.89 | -0.97 | -0.87 | 0.37 | 0.39 | 0.38 | 0.47 | 0.55 | 0.46 | 0.17 | 0.22 | 0.33 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 1.0 | 1.1 | 1.0 | 0.3 | 1.0 | 0.5 | 3.1 | 0.2 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.8 | -1.5 | -2.4 | -2.5 | -5.9 | -4.8 | -5.4 | -6.0 | -6.1 | -1.0 | 0.2 | 1.2 | 0.3 | 0 | 0.2 |
Endoplasmic Reticulum | -2.2 | 0.5 | 1.3 | 1.1 | 3.7 | 2.7 | 1.5 | 0.7 | 1.6 | -0.2 | -1.7 | -2.6 | -1.5 | -0.2 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 3.6 | 4.6 | 6.7 | 4.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 15.6883 | 27.0441 | 20.0037 | 21.3908 | 18.7886 | 20.3828 | 22.6926 | 33.4393 | 30.3142 | 25.2982 | 26.3264 | 30.2521 | 22.1459 | 33.8871 | 28.5361 | 23.9028 | 27.7314 | 28.0521 |
Actin | 0.0411 | 0.001 | 0.0402 | 0.0118 | 0.0246 | 0.0149 | 0.0224 | 0.0006 | 0.0073 | 0.0011 | 0.0256 | 0.0009 | 0.0191 | 0.0016 | 0.0079 | 0.0111 | 0.0003 | 0.0017 |
Bud | 0.0025 | 0.0008 | 0.002 | 0.0014 | 0.005 | 0.0018 | 0.0019 | 0.0003 | 0.0008 | 0.0015 | 0.0011 | 0.0014 | 0.0014 | 0.0002 | 0.001 | 0.0019 | 0.0003 | 0.0007 |
Bud Neck | 0.0036 | 0.0003 | 0.0017 | 0.0014 | 0.0008 | 0.0011 | 0.0008 | 0.0002 | 0.0005 | 0.001 | 0.0027 | 0.001 | 0.0007 | 0.0002 | 0.0004 | 0.0008 | 0.0006 | 0.0011 |
Bud Periphery | 0.0023 | 0.0003 | 0.0042 | 0.0004 | 0.0022 | 0.0016 | 0.0027 | 0.0002 | 0.0003 | 0.0007 | 0.0012 | 0.0011 | 0.0013 | 0.0001 | 0.0005 | 0.0006 | 0 | 0.0004 |
Bud Site | 0.0245 | 0.0008 | 0.0097 | 0.0012 | 0.0014 | 0.0008 | 0.0019 | 0.0002 | 0.0004 | 0.003 | 0.0022 | 0.0002 | 0.0019 | 0.0001 | 0.0011 | 0.0012 | 0.0001 | 0.0002 |
Cell Periphery | 0.0019 | 0.001 | 0.0025 | 0.0003 | 0.0004 | 0.0006 | 0.001 | 0.0004 | 0.0002 | 0.0007 | 0.0004 | 0.0006 | 0.0007 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 |
Cytoplasm | 0.564 | 0.9479 | 0.7284 | 0.6703 | 0.5988 | 0.67 | 0.7303 | 0.9569 | 0.9089 | 0.6885 | 0.734 | 0.8573 | 0.7515 | 0.9736 | 0.9033 | 0.7913 | 0.8781 | 0.8668 |
Cytoplasmic Foci | 0.0387 | 0.0028 | 0.0143 | 0.023 | 0.038 | 0.0115 | 0.0248 | 0.0034 | 0.0064 | 0.0218 | 0.0278 | 0.0134 | 0.0193 | 0.0023 | 0.0172 | 0.0217 | 0.0108 | 0.0243 |
Eisosomes | 0.0007 | 0 | 0.0014 | 0 | 0.0001 | 0.0003 | 0.0002 | 0 | 0 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0575 | 0.0213 | 0.0326 | 0.066 | 0.0532 | 0.0777 | 0.0309 | 0.0155 | 0.0111 | 0.0117 | 0.0271 | 0.0314 | 0.052 | 0.0097 | 0.0189 | 0.0043 | 0.0125 | 0.0199 |
Endosome | 0.0797 | 0.0063 | 0.0213 | 0.0591 | 0.0781 | 0.0622 | 0.047 | 0.0085 | 0.0221 | 0.0794 | 0.0883 | 0.0473 | 0.048 | 0.0039 | 0.0185 | 0.0213 | 0.0256 | 0.0465 |
Golgi | 0.0123 | 0.0015 | 0.0067 | 0.0043 | 0.0119 | 0.0114 | 0.0103 | 0.0012 | 0.0052 | 0.0058 | 0.0458 | 0.0109 | 0.0097 | 0.0034 | 0.003 | 0.0052 | 0.003 | 0.007 |
Lipid Particles | 0.0194 | 0.0004 | 0.0214 | 0.0016 | 0.0338 | 0.0018 | 0.0236 | 0.0007 | 0.0004 | 0.0181 | 0.0057 | 0.0003 | 0.0055 | 0 | 0.0022 | 0.0075 | 0.0494 | 0.0036 |
Mitochondria | 0.0216 | 0.0008 | 0.0391 | 0.0043 | 0.023 | 0.0707 | 0.0222 | 0.0004 | 0.0062 | 0.0279 | 0.0207 | 0.0093 | 0.0219 | 0.0008 | 0.0014 | 0.0041 | 0.0017 | 0.0102 |
None | 0.0111 | 0.0003 | 0.0035 | 0.0004 | 0.0027 | 0.0037 | 0.0024 | 0.0001 | 0.0002 | 0.0086 | 0.0005 | 0.0007 | 0.008 | 0.0002 | 0.0011 | 0.0019 | 0.0002 | 0.0029 |
Nuclear Periphery | 0.0266 | 0.0022 | 0.0055 | 0.0049 | 0.0357 | 0.037 | 0.0077 | 0.0007 | 0.0011 | 0.0053 | 0.0015 | 0.0015 | 0.0135 | 0.0008 | 0.0017 | 0.0018 | 0.0021 | 0.0011 |
Nucleolus | 0.0027 | 0 | 0.0017 | 0.0001 | 0.0017 | 0.0002 | 0.0011 | 0 | 0 | 0.0011 | 0.0001 | 0.0001 | 0.0008 | 0 | 0.0001 | 0.0002 | 0 | 0.0002 |
Nucleus | 0.0125 | 0.0012 | 0.0024 | 0.0022 | 0.0041 | 0.0044 | 0.017 | 0.0005 | 0.0007 | 0.0023 | 0.0009 | 0.001 | 0.0058 | 0.0005 | 0.001 | 0.0024 | 0.001 | 0.0009 |
Peroxisomes | 0.0085 | 0 | 0.0094 | 0.0263 | 0.0033 | 0.0013 | 0.0061 | 0 | 0.0002 | 0.0042 | 0.0014 | 0.0001 | 0.0027 | 0 | 0.0021 | 0.0407 | 0.0009 | 0.0008 |
Punctate Nuclear | 0.005 | 0 | 0.0018 | 0.0002 | 0.0273 | 0.0033 | 0.0016 | 0 | 0 | 0.0005 | 0.0003 | 0.0001 | 0.0024 | 0 | 0.0022 | 0.0008 | 0.0001 | 0.0003 |
Vacuole | 0.0479 | 0.0096 | 0.0387 | 0.0907 | 0.0341 | 0.0136 | 0.0372 | 0.0082 | 0.0205 | 0.0862 | 0.0087 | 0.0148 | 0.0244 | 0.0021 | 0.0128 | 0.0584 | 0.01 | 0.007 |
Vacuole Periphery | 0.0159 | 0.0016 | 0.0112 | 0.0301 | 0.0197 | 0.0101 | 0.0071 | 0.0019 | 0.0072 | 0.0302 | 0.0038 | 0.0065 | 0.0092 | 0.0004 | 0.0035 | 0.0226 | 0.0032 | 0.0043 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 114.0527 | 95.6248 | 89.1022 | 99.4383 | 86.128 | 111.7927 | 95.472 | 94.9052 | 86.4029 | 90.6555 |
Translational Efficiency | 1.0159 | 1.2746 | 1.1573 | 1.0323 | 1.072 | 0.9553 | 1.007 | 0.896 | 0.9613 | 0.9332 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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