Standard name
Human Ortholog
Description Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0.05 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.99 0.98 0.99 0.96 0.91 0.85 – 0.64 0.63 0.51 0.58 0.97 0.89 0.86 0.97 0.94 0.95 0.85 0.89 0.83 0.78 0.75 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0.12 – 0.35 0.39 0.48 0.35 0 0 0 0 0 0 0.05 0 0.07 0.09 0.09 0.06
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0.05 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 3 1 5 4 28 – 25 14 10 13 4 10 10 1 0 0 1 1 4 2 7 3
Bud 4 3 2 1 2 6 18 – 24 24 18 26 2 13 12 0 0 0 3 4 3 2 7 12
Bud Neck 3 0 13 10 18 6 21 – 6 1 2 3 0 0 0 0 1 1 1 1 2 1 3 4
Bud Site 0 0 0 0 0 1 4 – 2 2 2 1 1 0 1 0 0 0 – – – – – –
Cell Periphery 2 3 9 5 7 5 13 – 20 28 21 43 2 8 18 10 5 6 1 0 7 2 3 2
Cytoplasm 349 329 479 430 480 576 680 – 343 337 238 311 368 315 353 338 247 225 315 307 405 217 259 298
Endoplasmic Reticulum 2 3 6 2 2 1 9 – 7 11 7 8 2 3 8 24 14 16 2 1 2 3 7 3
Endosome 2 0 0 0 0 4 5 – 2 2 1 5 1 7 14 3 4 3 5 3 6 2 1 1
Golgi 1 0 0 0 0 0 0 – 1 0 1 0 0 0 0 2 1 1 1 1 1 1 0 2
Mitochondria 6 4 0 3 2 48 94 – 188 211 224 185 4 6 10 8 4 4 20 11 35 25 30 22
Nucleus 2 3 1 0 2 3 1 – 3 3 4 2 1 0 3 1 0 0 0 1 1 0 0 0
Nuclear Periphery 0 0 0 1 0 0 0 – 0 4 1 2 0 0 0 1 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 9 7 – 4 2 1 3 0 7 3 0 0 0 0 0 0 0 0 1
SpindlePole 2 0 3 2 10 11 35 – 16 7 3 15 1 4 9 1 2 1 9 1 7 1 2 3
Vac/Vac Membrane 1 0 1 0 0 8 7 – 4 16 28 28 1 2 4 2 6 6 4 4 2 13 16 14
Unique Cell Count 363 334 489 436 501 632 796 533 539 464 534 378 353 412 350 262 237 373 344 489 278 346 378
Labelled Cell Count 374 345 517 455 528 682 922 645 662 562 646 387 375 445 391 285 264 373 344 489 278 346 378


Cytoplasm, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.9 5.0 4.3 4.9 4.2 4.3 – 4.1 4.3 4.1 4.8 4.3 4.9 5.0 6.0 6.2 6.4 3.9 4.1 4.5
Std Deviation (1e-4) 1.7 1.0 0.9 1.0 1.1 1.2 1.1 – 1.2 1.2 1.0 1.1 1.0 1.7 1.4 1.4 1.6 1.9 1.1 1.3 1.8
Intensity Change (Log2) – – – -0.23 -0.03 -0.26 -0.21 – -0.27 -0.21 -0.28 -0.04 -0.21 -0.03 0.0 0.28 0.31 0.37 -0.36 -0.29 -0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.4073 3.041 2.56 1.8349 2.6807 2.5796 2.2107 1.9467 1.9231 1.4472 1.5903 1.954 3.6807 4.4277 4.3098 4.2976 4.224 3.9396
Actin 0.0408 0.0042 0.0329 0.0033 0.061 0.0011 0.0563 0.0067 0.0275 0.0035 0.035 0.0197 0.0005 0.0091 0.0079 0.018 0.0047 0.0049
Bud 0.0026 0.0019 0.0014 0.0013 0.0007 0.0011 0.0006 0.0021 0.0014 0.0011 0.0043 0.0017 0.0003 0.0018 0.0033 0.0027 0.0025 0.0006
Bud Neck 0.006 0.0004 0.007 0.0005 0.0006 0.0009 0.002 0.0003 0.0005 0.0006 0.0255 0.0053 0.0002 0.0006 0.0011 0.0004 0.0007 0.0017
Bud Periphery 0.0035 0.0014 0.0012 0.0014 0.0005 0.004 0.0007 0.001 0.0013 0.0021 0.0059 0.0023 0.0002 0.0009 0.0051 0.0049 0.0034 0.0009
Bud Site 0.0108 0.0071 0.0164 0.0012 0.0018 0.0003 0.0085 0.0189 0.0145 0.0012 0.0147 0.0084 0.0006 0.022 0.0064 0.0003 0.0013 0.0005
Cell Periphery 0.0009 0.0009 0.0006 0.0004 0.0003 0.0003 0.0003 0.0005 0.0004 0.0002 0.0004 0.0004 0.0002 0.0014 0.0013 0.0001 0.0004 0.0012
Cytoplasm 0.5733 0.8214 0.6977 0.7544 0.7978 0.8445 0.4947 0.7973 0.7697 0.6964 0.5943 0.7643 0.8308 0.8007 0.8375 0.75 0.8981 0.8787
Cytoplasmic Foci 0.0366 0.0264 0.0206 0.0194 0.0172 0.0172 0.0486 0.0161 0.0138 0.018 0.031 0.0328 0.0474 0.058 0.032 0.0468 0.0401 0.0315
Eisosomes 0.0003 0.0001 0.0002 0 0 0 0.0005 0.0002 0.0002 0.0001 0.0002 0.0001 0 0 0 0.0001 0 0.0001
Endoplasmic Reticulum 0.0108 0.0037 0.0066 0.0046 0.0032 0.0059 0.0054 0.0025 0.0047 0.0045 0.0037 0.0043 0.0093 0.0026 0.0059 0.003 0.0023 0.0049
Endosome 0.0606 0.0138 0.0186 0.0508 0.0419 0.029 0.0576 0.0048 0.0109 0.066 0.044 0.0182 0.0094 0.0109 0.0082 0.0072 0.0101 0.011
Golgi 0.0123 0.0034 0.0073 0.0119 0.0021 0.0026 0.035 0.0006 0.0026 0.0475 0.0224 0.009 0.0007 0.0017 0.0008 0.0013 0.0009 0.0016
Lipid Particles 0.0053 0.0014 0.0029 0.003 0.0014 0.0005 0.0304 0.0002 0.001 0.0013 0.0046 0.0016 0.0005 0.0005 0.0005 0.0007 0.0003 0.0004
Mitochondria 0.0212 0.0087 0.0043 0.0419 0.0005 0.0079 0.007 0.0003 0.0071 0.0073 0.0168 0.0113 0.0005 0.0003 0.0004 0.0006 0.0006 0.0009
None 0.1401 0.0912 0.1642 0.0887 0.055 0.0755 0.1596 0.143 0.1339 0.1373 0.1353 0.1105 0.0911 0.07 0.0804 0.1432 0.0265 0.0492
Nuclear Periphery 0.0104 0.001 0.0037 0.0017 0.001 0.0012 0.0194 0.0006 0.0012 0.0013 0.0022 0.0005 0.0014 0.0008 0.0013 0.0009 0.0009 0.001
Nucleolus 0.0012 0.0001 0.0003 0.0001 0.0001 0.0001 0.0011 0.0001 0.0002 0.0001 0.0004 0.0001 0.0001 0.0002 0.0001 0 0.0001 0.0001
Nucleus 0.0204 0.0016 0.003 0.0014 0.0013 0.0015 0.0415 0.0009 0.0011 0.001 0.0019 0.0013 0.0015 0.0019 0.002 0.0014 0.0016 0.0023
Peroxisomes 0.0118 0.006 0.0028 0.0041 0.0015 0.0005 0.0085 0.0002 0.0026 0.0007 0.0009 0.0052 0.0006 0.005 0.0012 0.0108 0.0016 0.0024
Punctate Nuclear 0.0117 0.0008 0.0045 0.0004 0.0005 0.0006 0.0164 0.0005 0.0009 0.0008 0.0014 0.0012 0.0009 0.009 0.001 0.0053 0.0012 0.0041
Vacuole 0.0147 0.0039 0.0031 0.0066 0.0107 0.0042 0.004 0.0029 0.0038 0.0071 0.0336 0.0015 0.0033 0.0025 0.0033 0.0019 0.0022 0.0017
Vacuole Periphery 0.0047 0.0005 0.0007 0.0026 0.001 0.0012 0.0018 0.0004 0.0005 0.002 0.0214 0.0004 0.0004 0.0003 0.0004 0.0004 0.0005 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.6313 1.855 10.6676 29.1754 14.1245 8.8141 9.7294 18.3551 32.9561 9.7909
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –
Standard name
Human Ortholog
Description Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
Localization
Cell Percentages cytoplasm (20%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Gsy1

Gsy1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gsy1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available