Standard name | |
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Human Ortholog | |
Description | Beta 7 subunit of the 20S proteasome |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | – | – | – | 0 | – | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | – | – | – | – | 0 | – | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 5 | 2 | 0 | 3 | 0 | 4 |
Bud Neck | 0 | 0 | – | – | – | – | 0 | – | – | – | – | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | – | – | – | 0 | – | – | – | – | 1 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | – | – | – | – | 0 | – | – | – | – | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 3 | – | – | – | – | 1 | – | – | – | – | 6 | – | 11 | 11 | 47 | 30 | 36 | 0 | 4 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | – | – | – | – | 0 | – | – | – | – | 0 | – | 0 | 0 | 10 | 8 | 14 | 0 | 0 | 0 | 1 | 0 | 0 |
Endosome | 0 | 1 | – | – | – | – | 0 | – | – | – | – | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Golgi | 0 | 0 | – | – | – | – | 0 | – | – | – | – | 0 | – | 0 | 5 | 9 | 12 | 22 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | – | – | – | – | 1 | – | – | – | – | 1 | – | 0 | 0 | 10 | 10 | 2 | 0 | 1 | 0 | 1 | 0 | 0 |
Nucleus | 17 | 76 | – | – | – | – | 4 | – | – | – | – | 14 | – | 29 | 23 | 230 | 153 | 123 | 83 | 115 | 23 | 63 | 84 | 83 |
Nuclear Periphery | 0 | 0 | – | – | – | – | 0 | – | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 12 | 4 | – | – | – | – | 3 | – | – | – | – | 12 | – | 6 | 2 | 23 | 14 | 22 | 6 | 9 | 2 | 7 | 13 | 14 |
Peroxisomes | 0 | 1 | – | – | – | – | 0 | – | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | – | – | – | – | 0 | – | – | – | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 10 | 22 | – | – | – | – | 12 | – | – | – | – | 9 | – | 23 | 37 | 89 | 43 | 70 | 5 | 11 | 6 | 8 | 23 | 19 |
Unique Cell Count | 37 | 105 | 20 | 41 | 65 | 75 | 386 | 249 | 255 | 103 | 148 | 35 | 92 | 124 | 129 | |||||||||
Labelled Cell Count | 40 | 107 | 21 | 43 | 69 | 79 | 418 | 272 | 289 | 103 | 148 | 35 | 92 | 124 | 129 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 67.9 | 60.8 | – | – | – | – | 32.9 | – | – | – | – | 54.8 | – | 73.1 | 76.3 | 44.2 | 57.4 | 57.1 | 60.7 | 80.6 | 70.7 |
Std Deviation (1e-4) | 21.7 | 22.6 | – | – | – | – | 13.6 | – | – | – | – | 32.0 | – | 30.8 | 30.1 | 18.0 | 21.9 | 23.4 | 31.6 | 24.3 | 28.0 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 32.8108 | 47.8298 | 43.0087 | 42.0485 | 38.6334 | 42.9878 | 32.8308 | 60.5479 | 55.8248 | 45.8759 | 56.836 | 57.8318 | 36.6697 | 52.3565 | 46.9986 | 45.1599 | 43.7899 | 47.9931 |
Actin | 0.0168 | 0 | 0.0012 | 0 | 0.0073 | 0.0064 | 0.0086 | 0.0011 | 0.0001 | 0.0099 | 0.0001 | 0.0003 | 0.0019 | 0 | 0.0003 | 0 | 0.0002 | 0.0001 |
Bud | 0.0008 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0011 | 0.0008 | 0.0002 | 0.0007 | 0.0024 | 0 | 0.0003 | 0.002 | 0 | 0.0038 | 0.0001 | 0 | 0.0002 |
Bud Neck | 0.0004 | 0.0001 | 0 | 0 | 0.0001 | 0.0015 | 0.0002 | 0.0003 | 0.0001 | 0.0005 | 0 | 0.0003 | 0.0007 | 0 | 0.0001 | 0 | 0.0002 | 0.0003 |
Bud Periphery | 0.0008 | 0 | 0.0002 | 0 | 0.0004 | 0.0013 | 0.0011 | 0.0003 | 0.0004 | 0.0033 | 0 | 0.0004 | 0.0035 | 0 | 0.0025 | 0 | 0.0001 | 0.0002 |
Bud Site | 0.0021 | 0.0002 | 0.0003 | 0 | 0.0003 | 0.0008 | 0.0017 | 0.001 | 0.0005 | 0.0109 | 0 | 0.0001 | 0.003 | 0 | 0.0011 | 0 | 0.0001 | 0.0002 |
Cell Periphery | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0009 | 0.0005 | 0.0001 | 0.0001 | 0.0017 | 0 | 0.0007 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.0207 | 0.0033 | 0.005 | 0.0005 | 0.0014 | 0.0082 | 0.0019 | 0.0011 | 0.0078 | 0.0005 | 0.0002 | 0.0003 | 0.0024 | 0.0012 | 0.0104 | 0.0006 | 0.0003 | 0.0029 |
Cytoplasmic Foci | 0.0046 | 0.0002 | 0.0011 | 0 | 0.0001 | 0.006 | 0.0186 | 0.0014 | 0.0059 | 0.0014 | 0 | 0.0003 | 0.0351 | 0 | 0.0013 | 0 | 0.0005 | 0.0001 |
Eisosomes | 0.0003 | 0 | 0 | 0 | 0.0005 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0009 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0026 | 0 | 0.001 | 0 | 0.0001 | 0.0023 | 0.0156 | 0.0001 | 0.0005 | 0.0002 | 0 | 0 | 0.0003 | 0 | 0.002 | 0 | 0 | 0.0002 |
Endosome | 0.031 | 0.0001 | 0.0094 | 0 | 0.0004 | 0.0078 | 0.0505 | 0.002 | 0.0233 | 0.0004 | 0 | 0.0002 | 0.0046 | 0 | 0.0083 | 0 | 0.0001 | 0.0004 |
Golgi | 0.0034 | 0 | 0.0004 | 0 | 0.0005 | 0.0013 | 0.0086 | 0.0004 | 0.0011 | 0.0007 | 0 | 0.0001 | 0.0012 | 0 | 0.0001 | 0 | 0 | 0 |
Lipid Particles | 0.0039 | 0 | 0.0001 | 0 | 0.0004 | 0.0009 | 0.0035 | 0.0008 | 0.0006 | 0.0019 | 0 | 0.0003 | 0.0045 | 0 | 0.0003 | 0 | 0.0002 | 0 |
Mitochondria | 0.0163 | 0.0009 | 0.0049 | 0.0003 | 0.1818 | 0.0098 | 0.0215 | 0.0081 | 0.0061 | 0.0185 | 0.002 | 0.0014 | 0.0149 | 0.0005 | 0.0045 | 0.0004 | 0.0018 | 0.0033 |
None | 0.0068 | 0.0002 | 0.0005 | 0 | 0.0002 | 0.0007 | 0.0036 | 0.0005 | 0.0006 | 0.0016 | 0 | 0.0001 | 0.0032 | 0 | 0.0013 | 0 | 0.0001 | 0.0002 |
Nuclear Periphery | 0.0476 | 0.0066 | 0.0237 | 0.005 | 0.0424 | 0.0355 | 0.0302 | 0.008 | 0.0041 | 0.0055 | 0.0146 | 0.0033 | 0.0408 | 0.0062 | 0.0111 | 0.0037 | 0.016 | 0.005 |
Nucleolus | 0.0523 | 0.0165 | 0.0203 | 0.0045 | 0.0206 | 0.0348 | 0.1211 | 0.0609 | 0.022 | 0.1903 | 0.0652 | 0.0345 | 0.082 | 0.0101 | 0.0288 | 0.0092 | 0.1231 | 0.0468 |
Nucleus | 0.7474 | 0.968 | 0.9291 | 0.9894 | 0.7382 | 0.864 | 0.6932 | 0.8974 | 0.9196 | 0.7359 | 0.9153 | 0.9542 | 0.6944 | 0.9812 | 0.9159 | 0.9857 | 0.8524 | 0.931 |
Peroxisomes | 0.0011 | 0 | 0.0001 | 0 | 0.0005 | 0.0003 | 0.0012 | 0.0002 | 0.0002 | 0.0012 | 0 | 0 | 0.0012 | 0 | 0.0001 | 0 | 0 | 0 |
Punctate Nuclear | 0.0305 | 0.0017 | 0.0005 | 0 | 0.0001 | 0.0086 | 0.0029 | 0.0145 | 0.0003 | 0.0115 | 0.0002 | 0.0022 | 0.0722 | 0.0001 | 0.0005 | 0.0001 | 0.0034 | 0.0068 |
Vacuole | 0.0059 | 0.0008 | 0.0005 | 0.0001 | 0.0008 | 0.0044 | 0.0058 | 0.0006 | 0.0033 | 0.0007 | 0.0004 | 0.0006 | 0.0191 | 0.0002 | 0.0038 | 0 | 0.0003 | 0.0009 |
Vacuole Periphery | 0.0043 | 0.001 | 0.0012 | 0.0001 | 0.0034 | 0.0038 | 0.0083 | 0.0007 | 0.0018 | 0.0013 | 0.0017 | 0.001 | 0.011 | 0.0003 | 0.0031 | 0.0001 | 0.001 | 0.0012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 134.9983 | 98.5145 | 112.3943 | 144.7983 | 99.1248 | 108.7649 | 139.7623 | 133.6169 | 127.65 | 122.2318 |
Translational Efficiency | 1.5381 | 1.7827 | 1.7347 | 1.292 | 1.6881 | 2.2174 | 1.5913 | 1.7289 | 1.8967 | 1.6287 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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