Standard name
Human Ortholog
Description Beta 7 subunit of the 20S proteasome

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.15 0.17 0.15 0.12 0.12 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0.07 0 0.05 0.09 0 0 0 0 0 0
Mitochondria 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.46 0.72 0.2 0.34 0.45 0.31 0.6 0.61 0.48 0.81 0.78 0.68 0.69 0.68 0.65
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.32 0 0.15 0.29 0.09 0 0.06 0.06 0.09 0.06 0.07 0.07 0.09 0.11 0.11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.27 0.21 0.6 0.22 0.35 0.49 0.23 0.17 0.27 0.05 0.08 0.19 0.09 0.19 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 5 2 0 3 0 4
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Bud Site 0 0 0 1 0 0 0 0 0
Cell Periphery 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 1 3 1 6 11 11 47 30 36 0 4 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 10 8 14 0 0 0 1 0 0
Endosome 0 1 0 0 0 0 0 1 0 0 0 0 1 0 1
Golgi 0 0 0 0 0 5 9 12 22 0 0 0 0 0 0
Mitochondria 0 0 1 1 0 0 10 10 2 0 1 0 1 0 0
Nucleus 17 76 4 14 29 23 230 153 123 83 115 23 63 84 83
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 12 4 3 12 6 2 23 14 22 6 9 2 7 13 14
Peroxisomes 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 10 22 12 9 23 37 89 43 70 5 11 6 8 23 19
Unique Cell Count 37 105 20 41 65 75 386 249 255 103 148 35 92 124 129
Labelled Cell Count 40 107 21 43 69 79 418 272 289 103 148 35 92 124 129


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 67.9 60.8 32.9 54.8 73.1 76.3 44.2 57.4 57.1 60.7 80.6 70.7
Std Deviation (1e-4) 21.7 22.6 13.6 32.0 30.8 30.1 18.0 21.9 23.4 31.6 24.3 28.0
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 32.8108 47.8298 43.0087 42.0485 38.6334 42.9878 32.8308 60.5479 55.8248 45.8759 56.836 57.8318 36.6697 52.3565 46.9986 45.1599 43.7899 47.9931
Actin 0.0168 0 0.0012 0 0.0073 0.0064 0.0086 0.0011 0.0001 0.0099 0.0001 0.0003 0.0019 0 0.0003 0 0.0002 0.0001
Bud 0.0008 0.0001 0.0003 0 0.0002 0.0011 0.0008 0.0002 0.0007 0.0024 0 0.0003 0.002 0 0.0038 0.0001 0 0.0002
Bud Neck 0.0004 0.0001 0 0 0.0001 0.0015 0.0002 0.0003 0.0001 0.0005 0 0.0003 0.0007 0 0.0001 0 0.0002 0.0003
Bud Periphery 0.0008 0 0.0002 0 0.0004 0.0013 0.0011 0.0003 0.0004 0.0033 0 0.0004 0.0035 0 0.0025 0 0.0001 0.0002
Bud Site 0.0021 0.0002 0.0003 0 0.0003 0.0008 0.0017 0.001 0.0005 0.0109 0 0.0001 0.003 0 0.0011 0 0.0001 0.0002
Cell Periphery 0.0004 0.0001 0.0001 0 0.0001 0.0003 0.0004 0.0001 0.0009 0.0005 0.0001 0.0001 0.0017 0 0.0007 0 0.0001 0.0001
Cytoplasm 0.0207 0.0033 0.005 0.0005 0.0014 0.0082 0.0019 0.0011 0.0078 0.0005 0.0002 0.0003 0.0024 0.0012 0.0104 0.0006 0.0003 0.0029
Cytoplasmic Foci 0.0046 0.0002 0.0011 0 0.0001 0.006 0.0186 0.0014 0.0059 0.0014 0 0.0003 0.0351 0 0.0013 0 0.0005 0.0001
Eisosomes 0.0003 0 0 0 0.0005 0.0001 0.0006 0.0001 0 0.0009 0 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0026 0 0.001 0 0.0001 0.0023 0.0156 0.0001 0.0005 0.0002 0 0 0.0003 0 0.002 0 0 0.0002
Endosome 0.031 0.0001 0.0094 0 0.0004 0.0078 0.0505 0.002 0.0233 0.0004 0 0.0002 0.0046 0 0.0083 0 0.0001 0.0004
Golgi 0.0034 0 0.0004 0 0.0005 0.0013 0.0086 0.0004 0.0011 0.0007 0 0.0001 0.0012 0 0.0001 0 0 0
Lipid Particles 0.0039 0 0.0001 0 0.0004 0.0009 0.0035 0.0008 0.0006 0.0019 0 0.0003 0.0045 0 0.0003 0 0.0002 0
Mitochondria 0.0163 0.0009 0.0049 0.0003 0.1818 0.0098 0.0215 0.0081 0.0061 0.0185 0.002 0.0014 0.0149 0.0005 0.0045 0.0004 0.0018 0.0033
None 0.0068 0.0002 0.0005 0 0.0002 0.0007 0.0036 0.0005 0.0006 0.0016 0 0.0001 0.0032 0 0.0013 0 0.0001 0.0002
Nuclear Periphery 0.0476 0.0066 0.0237 0.005 0.0424 0.0355 0.0302 0.008 0.0041 0.0055 0.0146 0.0033 0.0408 0.0062 0.0111 0.0037 0.016 0.005
Nucleolus 0.0523 0.0165 0.0203 0.0045 0.0206 0.0348 0.1211 0.0609 0.022 0.1903 0.0652 0.0345 0.082 0.0101 0.0288 0.0092 0.1231 0.0468
Nucleus 0.7474 0.968 0.9291 0.9894 0.7382 0.864 0.6932 0.8974 0.9196 0.7359 0.9153 0.9542 0.6944 0.9812 0.9159 0.9857 0.8524 0.931
Peroxisomes 0.0011 0 0.0001 0 0.0005 0.0003 0.0012 0.0002 0.0002 0.0012 0 0 0.0012 0 0.0001 0 0 0
Punctate Nuclear 0.0305 0.0017 0.0005 0 0.0001 0.0086 0.0029 0.0145 0.0003 0.0115 0.0002 0.0022 0.0722 0.0001 0.0005 0.0001 0.0034 0.0068
Vacuole 0.0059 0.0008 0.0005 0.0001 0.0008 0.0044 0.0058 0.0006 0.0033 0.0007 0.0004 0.0006 0.0191 0.0002 0.0038 0 0.0003 0.0009
Vacuole Periphery 0.0043 0.001 0.0012 0.0001 0.0034 0.0038 0.0083 0.0007 0.0018 0.0013 0.0017 0.001 0.011 0.0003 0.0031 0.0001 0.001 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 134.9983 98.5145 112.3943 144.7983 99.1248 108.7649 139.7623 133.6169 127.65 122.2318
Translational Efficiency 1.5381 1.7827 1.7347 1.292 1.6881 2.2174 1.5913 1.7289 1.8967 1.6287

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Beta 7 subunit of the 20S proteasome
Localization
Cell Percentages cytoplasm (43%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Pre4

Pre4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pre4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available