Standard name
Human Ortholog
Description Beta 7 subunit of the 20S proteasome
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – – – 0 – – – – 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – – – – 0 – – – – 0 – 0 0 0 0 0 0.05 0 0 0 0 0
Bud Neck 0 0 – – – – 0 – – – – 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – – – – 0 – – – – 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – – – – 0 – – – – 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 – – – – 0.05 – – – – 0.15 – 0.17 0.15 0.12 0.12 0.14 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 – – – – 0 – – – – 0 – 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 – – – – 0 – – – – 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – – – – 0 – – – – 0 – 0 0.07 0 0.05 0.09 0 0 0 0 0 0
Mitochondria 0 0 – – – – 0.05 – – – – 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.46 0.72 – – – – 0.2 – – – – 0.34 – 0.45 0.31 0.6 0.61 0.48 0.81 0.78 0.68 0.69 0.68 0.65
Nuclear Periphery 0 0 – – – – 0 – – – – 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.32 0 – – – – 0.15 – – – – 0.29 – 0.09 0 0.06 0.06 0.09 0.06 0.07 0.07 0.09 0.11 0.11
Peroxisomes 0 0 – – – – 0 – – – – 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – – – – 0 – – – – 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.27 0.21 – – – – 0.6 – – – – 0.22 – 0.35 0.49 0.23 0.17 0.27 0.05 0.08 0.19 0.09 0.19 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – – – 0 – – – – 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – – – – 0 – – – – 0 – 0 0 0 0 0 5 2 0 3 0 4
Bud Neck 0 0 – – – – 0 – – – – 0 – 0 0 0 1 0 0 0 0 0 0 0
Bud Site 0 0 – – – – 0 – – – – 1 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – – – – 0 – – – – 0 – 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 1 3 – – – – 1 – – – – 6 – 11 11 47 30 36 0 4 0 0 0 0
Endoplasmic Reticulum 0 0 – – – – 0 – – – – 0 – 0 0 10 8 14 0 0 0 1 0 0
Endosome 0 1 – – – – 0 – – – – 0 – 0 0 0 1 0 0 0 0 1 0 1
Golgi 0 0 – – – – 0 – – – – 0 – 0 5 9 12 22 0 0 0 0 0 0
Mitochondria 0 0 – – – – 1 – – – – 1 – 0 0 10 10 2 0 1 0 1 0 0
Nucleus 17 76 – – – – 4 – – – – 14 – 29 23 230 153 123 83 115 23 63 84 83
Nuclear Periphery 0 0 – – – – 0 – – – – 0 – 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 12 4 – – – – 3 – – – – 12 – 6 2 23 14 22 6 9 2 7 13 14
Peroxisomes 0 1 – – – – 0 – – – – 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – – – – 0 – – – – 0 – 0 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 10 22 – – – – 12 – – – – 9 – 23 37 89 43 70 5 11 6 8 23 19
Unique Cell Count 37 105 20 41 65 75 386 249 255 103 148 35 92 124 129
Labelled Cell Count 40 107 21 43 69 79 418 272 289 103 148 35 92 124 129


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 67.9 60.8 – – – – 32.9 – – – – 54.8 – 73.1 76.3 44.2 57.4 57.1 60.7 80.6 70.7
Std Deviation (1e-4) 21.7 22.6 – – – – 13.6 – – – – 32.0 – 30.8 30.1 18.0 21.9 23.4 31.6 24.3 28.0
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 32.8108 47.8298 43.0087 42.0485 38.6334 42.9878 32.8308 60.5479 55.8248 45.8759 56.836 57.8318 36.6697 52.3565 46.9986 45.1599 43.7899 47.9931
Actin 0.0168 0 0.0012 0 0.0073 0.0064 0.0086 0.0011 0.0001 0.0099 0.0001 0.0003 0.0019 0 0.0003 0 0.0002 0.0001
Bud 0.0008 0.0001 0.0003 0 0.0002 0.0011 0.0008 0.0002 0.0007 0.0024 0 0.0003 0.002 0 0.0038 0.0001 0 0.0002
Bud Neck 0.0004 0.0001 0 0 0.0001 0.0015 0.0002 0.0003 0.0001 0.0005 0 0.0003 0.0007 0 0.0001 0 0.0002 0.0003
Bud Periphery 0.0008 0 0.0002 0 0.0004 0.0013 0.0011 0.0003 0.0004 0.0033 0 0.0004 0.0035 0 0.0025 0 0.0001 0.0002
Bud Site 0.0021 0.0002 0.0003 0 0.0003 0.0008 0.0017 0.001 0.0005 0.0109 0 0.0001 0.003 0 0.0011 0 0.0001 0.0002
Cell Periphery 0.0004 0.0001 0.0001 0 0.0001 0.0003 0.0004 0.0001 0.0009 0.0005 0.0001 0.0001 0.0017 0 0.0007 0 0.0001 0.0001
Cytoplasm 0.0207 0.0033 0.005 0.0005 0.0014 0.0082 0.0019 0.0011 0.0078 0.0005 0.0002 0.0003 0.0024 0.0012 0.0104 0.0006 0.0003 0.0029
Cytoplasmic Foci 0.0046 0.0002 0.0011 0 0.0001 0.006 0.0186 0.0014 0.0059 0.0014 0 0.0003 0.0351 0 0.0013 0 0.0005 0.0001
Eisosomes 0.0003 0 0 0 0.0005 0.0001 0.0006 0.0001 0 0.0009 0 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0026 0 0.001 0 0.0001 0.0023 0.0156 0.0001 0.0005 0.0002 0 0 0.0003 0 0.002 0 0 0.0002
Endosome 0.031 0.0001 0.0094 0 0.0004 0.0078 0.0505 0.002 0.0233 0.0004 0 0.0002 0.0046 0 0.0083 0 0.0001 0.0004
Golgi 0.0034 0 0.0004 0 0.0005 0.0013 0.0086 0.0004 0.0011 0.0007 0 0.0001 0.0012 0 0.0001 0 0 0
Lipid Particles 0.0039 0 0.0001 0 0.0004 0.0009 0.0035 0.0008 0.0006 0.0019 0 0.0003 0.0045 0 0.0003 0 0.0002 0
Mitochondria 0.0163 0.0009 0.0049 0.0003 0.1818 0.0098 0.0215 0.0081 0.0061 0.0185 0.002 0.0014 0.0149 0.0005 0.0045 0.0004 0.0018 0.0033
None 0.0068 0.0002 0.0005 0 0.0002 0.0007 0.0036 0.0005 0.0006 0.0016 0 0.0001 0.0032 0 0.0013 0 0.0001 0.0002
Nuclear Periphery 0.0476 0.0066 0.0237 0.005 0.0424 0.0355 0.0302 0.008 0.0041 0.0055 0.0146 0.0033 0.0408 0.0062 0.0111 0.0037 0.016 0.005
Nucleolus 0.0523 0.0165 0.0203 0.0045 0.0206 0.0348 0.1211 0.0609 0.022 0.1903 0.0652 0.0345 0.082 0.0101 0.0288 0.0092 0.1231 0.0468
Nucleus 0.7474 0.968 0.9291 0.9894 0.7382 0.864 0.6932 0.8974 0.9196 0.7359 0.9153 0.9542 0.6944 0.9812 0.9159 0.9857 0.8524 0.931
Peroxisomes 0.0011 0 0.0001 0 0.0005 0.0003 0.0012 0.0002 0.0002 0.0012 0 0 0.0012 0 0.0001 0 0 0
Punctate Nuclear 0.0305 0.0017 0.0005 0 0.0001 0.0086 0.0029 0.0145 0.0003 0.0115 0.0002 0.0022 0.0722 0.0001 0.0005 0.0001 0.0034 0.0068
Vacuole 0.0059 0.0008 0.0005 0.0001 0.0008 0.0044 0.0058 0.0006 0.0033 0.0007 0.0004 0.0006 0.0191 0.0002 0.0038 0 0.0003 0.0009
Vacuole Periphery 0.0043 0.001 0.0012 0.0001 0.0034 0.0038 0.0083 0.0007 0.0018 0.0013 0.0017 0.001 0.011 0.0003 0.0031 0.0001 0.001 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 134.9983 98.5145 112.3943 144.7983 99.1248 108.7649 139.7623 133.6169 127.65 122.2318
Translational Efficiency 1.5381 1.7827 1.7347 1.292 1.6881 2.2174 1.5913 1.7289 1.8967 1.6287

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
24 712 390 2829 2771 2569 2115 2029 2795 3281 2505 4858

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 735.65 652.75 827.89 871.55 673.23 780.56 745.08 754.30 673.77 752.82 757.97 822.58
Standard Deviation 199.94 213.51 99.11 103.94 71.14 94.15 90.52 94.03 73.44 140.03 96.69 115.45
Intensity Change Log 2 — -0.172489 0.170420 0.244564 — 0.213410 0.146296 0.164039 — 0.024802 0.158942 0.206645

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000737 0.000367 0.000331 0.000295 0.000060 0.000163 0.000059 0.000191 0.000065 0.000207 0.000101 0.000251
Bud Neck 0.010485 0.018655 0.002539 0.021939 0.009732 0.020387 0.005570 0.007770 0.009739 0.020011 0.005098 0.016021
Bud Site 0.015322 0.009786 0.005194 0.028469 0.003838 0.010590 0.003683 0.020578 0.003937 0.010416 0.003918 0.025173
Cell Periphery 0.000339 0.000180 0.000199 0.000137 0.000143 0.000182 0.000822 0.000385 0.000145 0.000181 0.000725 0.000240
Cytoplasm 0.338762* 0.461887* 0.191258* 0.557250* 0.538688* 0.511377* 0.374239* 0.434434* 0.536972* 0.500637* 0.345751* 0.505955*
Cytoplasmic Foci 0.058866 0.225065* 0.011664 0.002992 0.120795 0.115860 0.016281 0.015246 0.120264 0.139559 0.015562 0.008110
Eisosomes 0.000136 0.000256 0.000074 0.000016 0.000144 0.000112 0.000371 0.000047 0.000144 0.000143 0.000325 0.000029
Endoplasmic Reticulum 0.009547 0.000818 0.004222 0.000780 0.000561 0.000357 0.000678 0.001059 0.000638 0.000457 0.001229 0.000896
Endosome 0.028380 0.013934 0.003558 0.001101 0.002684 0.007812 0.001480 0.002110 0.002904 0.009140 0.001803 0.001523
Golgi 0.001551 0.002924 0.000183 0.000153 0.001263 0.001760 0.000306 0.000222 0.001265 0.002013 0.000287 0.000182
Lipid Particles 0.005549 0.007997 0.003106 0.000371 0.004897 0.004216 0.001971 0.000942 0.004902 0.005036 0.002148 0.000609
Mitochondria 0.001492 0.005388 0.000519 0.005256 0.001539 0.005162 0.000748 0.000759 0.001539 0.005211 0.000712 0.003378
Mitotic Spindle 0.001570 0.001452 0.002075 0.039952 0.000150 0.008353 0.004583 0.004612 0.000162 0.006855 0.004193 0.025192
None 0.056920 0.019114 0.010241 0.006046 0.003032 0.005850 0.015619 0.012820 0.003495 0.008728 0.014782 0.008875
Nuclear Periphery 0.006083 0.000498 0.002062 0.001803 0.000329 0.000535 0.001027 0.001224 0.000378 0.000527 0.001188 0.001562
Nuclear Periphery Foci 0.014061 0.000425 0.001039 0.000099 0.000191 0.000143 0.000287 0.000376 0.000310 0.000204 0.000404 0.000215
Nucleolus 0.022847 0.003747 0.001943 0.000674 0.000559 0.001616 0.000965 0.000955 0.000750 0.002079 0.001117 0.000791
Nucleus 0.346075* 0.163256* 0.709631* 0.295614* 0.286619* 0.255912* 0.549322* 0.458645* 0.287130* 0.235805* 0.574280* 0.363706*
Peroxisomes 0.010574 0.009570 0.000458 0.000447 0.002043 0.004377 0.000411 0.000259 0.002116 0.005504 0.000419 0.000368
Vacuole 0.067915 0.053587 0.049424 0.035968 0.022475 0.044122 0.021389 0.036840 0.022865 0.046176 0.025754 0.036332
Vacuole Periphery 0.002790 0.001094 0.000279 0.000636 0.000257 0.001114 0.000188 0.000525 0.000279 0.001110 0.000202 0.000590

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.11 0.55 1.58 1.58 3.35 -2.59 -1.23 -7.16 -1.11 -6.54 -3.87 -4.54 -4.77 -0.13 -1.93
Bud Neck -1.68 1.70 -1.41 0.77 -11.52 -8.32 4.73 2.38 9.31 -1.78 -9.03 5.54 -4.04 5.85 -9.00
Bud Site 0.94 1.51 -0.03 -2.94 -4.21 -5.34 0.02 -7.25 -3.43 -7.37 -5.64 -0.18 -10.29 -4.56 -10.48
Cell Periphery 2.94 2.31 4.75 5.36 5.95 -1.69 -7.64 -4.12 -3.45 4.55 -1.67 -7.66 -1.72 -0.41 6.99
Cytoplasm -2.00 2.65 -2.06 -0.20 -26.21 4.21 25.88 19.56 14.80 -4.46 6.00 31.31 18.00 11.11 -16.24
Cytoplasmic Foci -6.34 2.54 2.87 27.48 5.96 1.10 35.00 35.65 32.67 0.88 -4.57 35.65 38.96 43.10 7.92
Eisosomes -1.50 1.02 3.29 3.35 11.74 3.12 -2.46 9.41 13.95 3.91 0.15 -2.33 11.38 7.12 4.35
Endoplasmic Reticulum 1.34 0.29 1.29 -1.42 9.80 2.91 -6.31 -10.83 -15.15 -4.11 1.85 -10.00 -7.13 -12.05 6.17
Endosome 1.16 1.87 2.10 8.83 6.73 -8.23 4.40 2.25 9.05 -1.48 -10.19 3.51 6.38 13.49 2.95
Golgi -0.99 1.68 1.75 4.22 1.27 -1.17 4.45 6.08 4.11 0.43 -1.94 4.88 6.70 5.64 0.81
Lipid Particles -1.31 1.26 2.95 9.20 9.37 1.33 5.73 10.34 11.17 1.98 -0.38 5.75 11.37 15.19 3.98
Mitochondria -2.61 2.40 -2.65 1.48 -7.52 -5.74 4.78 6.42 7.02 0.28 -6.40 5.47 -3.02 4.90 -6.38
Mitotic Spindle 0.00 0.13 -8.61 -9.21 -9.52 -6.19 -4.05 -6.68 2.23 -0.39 -6.44 -4.31 -13.40 -5.85 -8.14
None 1.17 1.54 1.59 3.85 0.73 -4.28 -6.82 -6.60 -3.40 1.64 -5.61 -6.70 -7.60 0.83 3.30
Nuclear Periphery 1.01 0.55 0.45 -24.48 -2.95 -2.69 -19.14 -19.80 -12.25 -3.11 -2.02 -18.35 -27.83 -24.19 -11.44
Nuclear Periphery Foci 1.20 1.08 1.23 6.23 6.16 0.53 -0.99 -2.37 -5.04 -1.43 0.79 -1.42 0.40 -1.63 3.32
Nucleolus 1.96 2.20 2.27 3.45 2.74 -5.78 -3.24 -3.72 3.54 0.07 -5.29 -1.89 -0.73 5.17 1.50
Nucleus 2.59 -5.14 -0.75 -24.83 20.87 5.32 -38.14 -26.20 -29.06 7.92 9.31 -43.19 -25.90 -34.74 21.36
Peroxisomes 0.54 1.90 1.93 7.90 2.92 -5.27 5.94 6.86 11.03 1.26 -7.21 6.28 7.13 13.48 1.62
Vacuole 0.43 -1.00 -0.13 -3.08 2.75 -12.54 -5.70 -13.19 -3.46 -7.91 -14.67 -9.50 -22.67 -7.18 -8.93
Vacuole Periphery 1.72 2.56 2.48 3.75 -1.19 -4.28 2.63 -2.11 3.06 -2.89 -5.08 2.97 -2.26 4.26 -3.84
Standard name
Human Ortholog
Description Beta 7 subunit of the 20S proteasome
Localization
Cell Percentages cytoplasm (43%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Pre4

Pre4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pre4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available