Standard name
Human Ortholog
Description Isopropylmalate isomerase; catalyzes the second step in the leucine biosynthesis pathway
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.1 0.11 0.13 0.08 0 0 0 0 0 0 0 0 0.13 0.25 0.36 0.32 0.48 0.21 0 0 0 0 0 0
Cytoplasm 0.96 0.92 0.94 0.97 0.99 0.99 0.99 0.95 0.96 0.89 0.88 0.9 0.94 0.96 0.77 0.78 0.64 0.84 0.91 0.91 0.93 0.89 0.75 0.76
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0.07 0.07 0.05 0 0 0 0 0 0.09 0 0 0 0 0.17 0.12
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 12 11 18 8 4 1 1 3 1 4 1 6 9 14 8 71 86 30 6 4 0 0 0 1
Cytoplasm 118 89 130 92 155 120 182 175 92 115 108 130 66 54 17 173 115 122 141 139 157 73 95 90
Endoplasmic Reticulum 0 0 0 0 0 0 1 4 1 9 8 7 0 0 0 2 5 13 2 1 4 3 21 14
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 2 2 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3
Nucleolus 0 0 0 0 0 0 0 3 2 4 5 3 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 1 0 0 0 1 1 0 0 3
Unique Cell Count 123 97 138 95 157 121 184 184 96 129 123 145 70 56 22 221 181 145 155 153 170 82 128 120
Labelled Cell Count 130 100 148 100 160 121 184 185 96 132 126 146 75 68 25 249 208 166 155 153 170 82 128 120


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 558.6 533.9 446.9 415.0 376.4 309.2 268.7 246.9 230.8 203.1 186.5 192.5 529.6 471.7 511.5 591.5 636.3 575.8 434.6 488.8 500.8
Std Deviation (1e-4) 83.2 90.7 103.3 94.8 77.8 69.0 60.3 65.1 65.7 50.9 47.4 56.0 137.2 144.0 74.6 173.3 152.9 159.8 164.3 88.6 95.1
Intensity Change (Log2) – – – -0.11 -0.25 -0.53 -0.73 -0.86 -0.95 -1.14 -1.26 -1.21 0.24 0.08 0.19 0.4 0.51 0.37 -0.04 0.13 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.1 -3.4 -3.8 -4.7 -4.1 -3.3 -3.0 -3.8 -2.7 0 2.0 0 4.1 6.5 1.7
Cytoplasm 0.9 2.1 2.2 2.4 0.4 0.6 -1.5 -1.8 -1.4 0 0.6 -2.7 -4.0 -6.4 -2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 325.5561 616.2823 497.2635 583.1348 225.4459 544.9735 495.0411 840.4164 700.2923 741.5354 579.9003 688.723 454.1036 741.3732 623.3236 705.5496 321.5243 662.7188
Actin 0.0272 0.0001 0.0287 0.0001 0.001 0.0002 0.0054 0.0001 0.0002 0.0001 0.0001 0.0001 0.0229 0.0009 0.003 0 0.0907 0.0001
Bud 0.0058 0.0002 0.002 0.0001 0.0071 0.0004 0.0084 0.0002 0.0003 0 0.0001 0.0002 0.0071 0.0003 0.0009 0 0.0027 0.0001
Bud Neck 0.0009 0.0001 0.0005 0.0001 0.0001 0.0003 0.0007 0 0.0001 0.0001 0.0002 0.0002 0.0005 0.0001 0.0002 0.0001 0.0034 0.0002
Bud Periphery 0.0048 0 0.0011 0 0.0014 0 0.0017 0 0.0001 0 0 0 0.0019 0.0002 0.0004 0 0.0032 0
Bud Site 0.0072 0 0.0008 0 0.0012 0 0.0027 0 0.0001 0 0 0 0.0022 0.0003 0.0012 0 0.0038 0
Cell Periphery 0.0172 0.0001 0.0003 0 0.001 0.0001 0.0014 0 0.0002 0 0 0 0.0014 0.0001 0.0003 0 0.007 0
Cytoplasm 0.5765 0.9737 0.8565 0.8231 0.5258 0.8983 0.6722 0.9706 0.8377 0.8247 0.9623 0.9089 0.7175 0.9756 0.8624 0.9293 0.5417 0.9509
Cytoplasmic Foci 0.0478 0 0.0081 0 0.0316 0.0001 0.035 0 0.0169 0 0.0001 0.0001 0.026 0.0004 0.0214 0 0.0295 0
Eisosomes 0.0013 0 0.0002 0 0.0012 0 0.0012 0 0.0002 0 0 0 0.0016 0 0.0004 0 0.0014 0
Endoplasmic Reticulum 0.0049 0 0.0026 0 0.0002 0.0001 0.0023 0 0.0002 0.0001 0.0001 0 0.0102 0.0003 0.0021 0 0.0278 0
Endosome 0.0159 0 0.0135 0 0.0001 0 0.0053 0 0.0003 0 0 0 0.0143 0.0004 0.0059 0 0.072 0
Golgi 0.0054 0 0.0103 0 0.0001 0 0.0006 0 0 0 0 0 0.0043 0.0001 0.0076 0 0.0161 0
Lipid Particles 0.0084 0 0.0047 0 0.0009 0 0.002 0 0.0008 0 0 0 0.0049 0 0.0023 0 0.0119 0
Mitochondria 0.0274 0.0004 0.0127 0.0012 0.2514 0.0009 0.0527 0.0005 0.0303 0.0009 0.0003 0.0004 0.0324 0.0022 0.0147 0.0002 0.0362 0.0002
None 0.0195 0.0003 0.0076 0.0002 0.0979 0.0004 0.0316 0.0002 0.0244 0.0003 0.0002 0.0004 0.0222 0.0014 0.0175 0.0002 0.0673 0.0002
Nuclear Periphery 0.0377 0.0019 0.012 0.0033 0.0011 0.0066 0.0226 0.0008 0.0037 0.0065 0.0039 0.0031 0.0248 0.0017 0.0024 0.0066 0.0176 0.003
Nucleolus 0.0474 0.0001 0.0013 0.0006 0.0415 0.0003 0.0309 0.0001 0.0105 0 0.0001 0.0001 0.0334 0.0019 0.0094 0.0002 0.0237 0
Nucleus 0.0953 0.0228 0.029 0.1707 0.0219 0.0912 0.1092 0.0272 0.071 0.1671 0.0321 0.0861 0.0615 0.0137 0.0437 0.063 0.0187 0.045
Peroxisomes 0.0047 0 0.0011 0 0.0053 0 0.0021 0 0.0005 0 0 0 0.0018 0 0.0011 0 0.0024 0
Punctate Nuclear 0.0219 0.0001 0.001 0.0001 0.0008 0.0002 0.0044 0 0.001 0.0002 0.0001 0.0002 0.0024 0.0001 0.0006 0.0001 0.0019 0.0001
Vacuole 0.0184 0.0001 0.0038 0.0001 0.0036 0.0004 0.0034 0.0001 0.0005 0 0.0001 0.0001 0.004 0.0002 0.0014 0.0001 0.0123 0.0001
Vacuole Periphery 0.0043 0 0.0023 0.0002 0.0048 0.0004 0.0042 0.0001 0.0009 0.0001 0.0001 0.0001 0.0028 0.0001 0.0014 0.0001 0.0088 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 122.3647 86.8348 68.5015 69.2435 69.6965 77.7611 66.8079 59.1143 56.3838 61.6959
Translational Efficiency 1.0318 1.3836 1.2407 1.3744 1.4323 1.2425 1.1607 1.0483 1.0737 1.06

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1776 87 652 1092 198 1420 1572 778 1974 1507 2224 1870

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 32239.95 33638.20 37742.74 25507.47 39975.60 31889.86 32743.50 33146.02 33015.87 31990.79 34209.10 28685.43
Standard Deviation 6502.17 8142.74 6753.88 6813.79 7812.73 7808.12 6738.53 7978.72 7039.90 7838.44 7116.70 8232.42
Intensity Change Log 2 — 0.061251 0.227350 -0.337930 — -0.326022 -0.287911 -0.270284 — -0.140197 -0.034968 -0.300093

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000056 0.000103 0.000107 0.000232 0.000090 0.000091 0.000069 0.000031 0.000059 0.000092 0.000080 0.000148
Bud Neck 0.000401 0.002792 0.002050 0.003663 0.001020 0.000527 0.000928 0.000549 0.000463 0.000658 0.001257 0.002368
Bud Site 0.000090 0.000492 0.000246 0.003282 0.000438 0.000554 0.000234 0.000130 0.000125 0.000551 0.000237 0.001971
Cell Periphery 0.000614 0.007067 0.043576 0.000493 0.074299 0.002346 0.004248 0.000139 0.008005 0.002619 0.015777 0.000346
Cytoplasm 0.515801* 0.456982* 0.581206* 0.178636* 0.577130* 0.595003* 0.554868* 0.572467* 0.521952* 0.587035* 0.562589* 0.342487*
Cytoplasmic Foci 0.000603 0.003564 0.001556 0.007871 0.001048 0.001264 0.000605 0.000193 0.000648 0.001396 0.000884 0.004677
Eisosomes 0.000001 0.000004 0.000009 0.000007 0.000010 0.000001 0.000001 0.000001 0.000002 0.000002 0.000003 0.000004
Endoplasmic Reticulum 0.000484 0.000167 0.000170 0.000222 0.000429 0.000585 0.000182 0.000275 0.000479 0.000561 0.000178 0.000244
Endosome 0.000013 0.000115 0.000048 0.002102 0.000071 0.000051 0.000027 0.000026 0.000019 0.000055 0.000033 0.001238
Golgi 0.000173 0.001781 0.001373 0.001113 0.001486 0.000318 0.000106 0.000010 0.000305 0.000402 0.000477 0.000654
Lipid Particles 0.000082 0.005537 0.005335 0.000209 0.005125 0.000667 0.000610 0.000044 0.000588 0.000948 0.001996 0.000140
Mitochondria 0.000395 0.000393 0.000218 0.001160 0.000173 0.000394 0.000337 0.000328 0.000373 0.000394 0.000302 0.000814
Mitotic Spindle 0.000016 0.010020 0.000048 0.004962 0.000021 0.000292 0.000076 0.000021 0.000016 0.000853 0.000068 0.002906
None 0.007874 0.009453 0.004321 0.014050 0.001844 0.008003 0.003165 0.012965 0.007269 0.008087 0.003504 0.013599
Nuclear Periphery 0.000301 0.005399 0.001544 0.004601 0.001105 0.000523 0.000661 0.004675 0.000382 0.000804 0.000920 0.004632
Nuclear Periphery Foci 0.000378 0.011159 0.002369 0.000567 0.002320 0.000840 0.000403 0.000141 0.000573 0.001435 0.000980 0.000390
Nucleolus 0.000113 0.000097 0.000057 0.001198 0.000109 0.000113 0.000079 0.000069 0.000112 0.000112 0.000073 0.000728
Nucleus 0.465740* 0.443265* 0.159793* 0.767472* 0.100644 0.369318* 0.394053* 0.382988* 0.429120* 0.373587* 0.325376* 0.607510*
Peroxisomes 0.000002 0.000005 0.000004 0.000198 0.000010 0.000007 0.000003 0.000002 0.000003 0.000007 0.000003 0.000116
Vacuole 0.005142 0.039930 0.191785 0.005798 0.228412* 0.018572 0.039135 0.024916 0.027537 0.019805 0.083887 0.013752
Vacuole Periphery 0.001720 0.001677 0.004184 0.002164 0.004217 0.000531 0.000209 0.000028 0.001970 0.000597 0.001375 0.001276

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.99 -5.88 -8.29 -1.89 -2.79 0.03 -0.02 4.34 3.93 13.62 -2.17 -5.11 -5.52 -1.68 -1.74
Bud Neck -2.71 -8.45 -11.49 -1.53 -5.42 2.20 -4.24 1.97 -0.38 7.47 -3.16 -11.39 -11.19 -9.65 -3.86
Bud Site -1.94 -5.55 -3.33 -2.31 -2.89 -0.39 0.48 2.23 1.22 4.78 -1.40 -6.38 -3.30 -1.25 -2.52
Cell Periphery -4.16 -12.47 1.04 4.24 12.51 8.19 8.12 8.52 8.55 5.69 4.54 -4.44 7.65 8.60 12.82
Cytoplasm 2.45 -2.64 36.32 7.82 27.88 0.10 4.46 1.46 2.58 -5.72 -4.87 1.96 19.63 22.89 18.24
Cytoplasmic Foci -2.87 -3.09 -5.10 1.03 -2.27 -0.68 -0.15 2.81 4.68 6.56 -3.68 -4.18 -4.30 -1.01 -1.89
Eisosomes -3.44 -7.82 -8.59 -1.00 1.84 3.08 2.72 3.73 5.26 7.01 -0.34 -4.72 -4.70 -5.92 0.06
Endoplasmic Reticulum 1.85 1.82 1.60 -0.88 -0.98 -0.56 2.23 1.49 1.25 -1.38 -0.25 2.08 1.69 1.28 -1.88
Endosome -2.22 -5.48 -1.67 -1.29 -1.53 0.76 1.26 2.05 1.11 3.36 -1.78 -5.59 -1.67 -1.47 -1.51
Golgi -3.07 -8.47 -1.09 2.68 5.11 3.08 3.72 4.08 7.22 11.20 -1.90 -3.30 0.90 2.17 3.03
Lipid Particles -2.93 -9.80 -4.88 2.68 8.95 4.83 4.56 5.79 5.37 7.64 -2.61 -8.61 1.95 4.00 10.12
Mitochondria -0.64 2.28 -9.11 -5.14 -11.75 -3.66 -5.28 -1.61 1.91 3.10 -0.70 -0.53 -7.02 -6.68 -8.31
Mitotic Spindle -1.06 -1.16 -2.55 0.67 -2.51 -1.14 -2.13 -1.42 0.10 -0.59 -1.48 -2.26 -2.69 -1.28 -2.51
None 0.04 2.19 -0.80 -0.39 -2.78 -4.39 -2.13 -5.10 -1.34 -4.54 -0.29 3.84 -1.82 -1.29 -6.16
Nuclear Periphery -2.31 -7.10 -5.07 -0.37 -3.71 1.48 -0.72 -2.15 -2.66 -1.93 -2.99 -5.92 -5.74 -5.12 -4.35
Nuclear Periphery Foci -1.97 -7.72 -3.01 1.79 3.47 3.67 4.46 5.84 4.55 6.25 -2.44 -4.74 -1.27 1.62 2.21
Nucleolus -0.44 0.93 -2.04 -1.96 -2.21 -0.10 -0.27 0.44 0.44 1.24 -0.33 -0.04 -2.01 -1.93 -2.05
Nucleus 0.63 22.43 -28.78 -8.74 -47.90* -15.47 -20.84 -16.86 -2.35 4.30 4.47 4.82 -18.19 -21.33 -23.57
Peroxisomes -1.77 -1.72 -2.77 -2.59 -2.71 -0.11 1.88 2.52 1.85 2.67 -1.77 -1.35 -2.72 -2.20 -2.65
Vacuole -3.90 -18.23 -6.77 2.89 16.67 10.49 9.37 10.20 -0.97 3.79 1.10 -12.89 1.68 0.58 15.02
Vacuole Periphery -0.15 -2.67 1.44 1.44 4.71 3.54 3.98 4.16 3.64 9.19 2.17 1.05 2.49 0.96 3.04
Standard name
Human Ortholog
Description Isopropylmalate isomerase; catalyzes the second step in the leucine biosynthesis pathway
Localization
Cell Percentages cytoplasm (91%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Leu1

Leu1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Leu1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available