Standard name
Human Ortholog
Description Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; relocalizes to the cytosol in response to hypoxia; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.05 0 0.06 0 0 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.12 0.15 0.07 0.06 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.47 0.57 0.63 0.31 0.55 0.51 0.56 0.74 0.73 0.72 0.75 0.05 0 0.17 0 0 0.09 0 0 0 0 0 0
Nucleus 0.94 0.87 0.88 0.88 0.86 0.78 0.75 0.74 0.62 0.65 0.66 0.65 0.85 0.81 0.78 0.85 0.88 0.86 0.81 0.89 0.77 0.76 0.72 0.52
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0.06 0 0.05 0.08 0.12 0.12 0.19 0.16 0.18 0.19 0.19 0 0.05 0 0 0 0 0.09 0 0.07 0.06 0.09 0.15
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.1 0.05 0.11 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0
Bud 0 0 2 1 2 4 1 6 4 8 3 4 0 1 0 1 0 2 0 1 1 0 2 3
Bud Neck 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 4 0 6 22 19 43 13 15 8 26 0 1 1 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 1 5 4 6 8 9 8 9 8 7 6 17 32 45 52 25 40 0 0 1 0 1 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 11 4 13 0 0 0 0 0 1
Endosome 1 0 0 0 1 0 0 0 0 0 0 0 0 0 1 11 4 6 1 0 1 2 3 10
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 1 0 0 0 1 3 1
Mitochondria 4 63 139 152 116 233 298 405 292 327 262 347 9 8 49 30 11 55 4 0 2 1 3 7
Nucleus 159 116 213 212 328 331 442 531 246 292 240 301 142 214 230 608 369 511 157 117 186 72 189 146
Nuclear Periphery 1 0 0 0 2 12 24 13 6 14 14 11 0 3 0 2 0 4 1 0 0 0 0 2
Nucleolus 9 8 8 11 32 49 73 136 62 82 68 90 7 12 12 18 18 19 17 5 16 5 24 41
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 3 2 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 3 2 4 4 7 16 0 4 3 3 0 2 2 9 5 6 1 0 4 1 3 11
Vac/Vac Membrane 1 4 2 2 2 1 1 0 3 0 0 5 1 3 3 26 9 19 6 6 23 4 28 45
Unique Cell Count 170 133 243 240 380 426 586 717 397 450 365 465 168 263 295 717 418 591 193 132 242 96 264 281
Labelled Cell Count 178 192 376 384 499 664 874 1161 636 751 606 794 176 283 345 769 446 679 193 132 242 96 264 281


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 4.3 3.8 3.8 4.3 3.8 3.8 3.8 3.3 3.4 3.3 3.2 5.8 6.0 5.2 7.3 7.7 8.2 5.2 5.6 6.0
Std Deviation (1e-4) 1.4 1.4 0.5 0.8 0.8 0.8 0.7 0.9 0.8 0.8 1.0 1.1 1.4 1.6 1.4 1.4 1.6 1.6 0.9 1.2 1.1
Intensity Change (Log2) – – – -0.0 0.17 0.01 -0.01 0.02 -0.19 -0.15 -0.17 -0.25 0.63 0.68 0.45 0.95 1.04 1.11 0.46 0.56 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – 0
Bud Neck – – – – – – – – – – – – 0 – 0
Bud Site – – – – – – – – – – – – 0 – 0
Cell Periphery – – – – – – – – – – – – 0 – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – 0
Endosome – – – – – – – – – – – – 0 – 0
Golgi – – – – – – – – – – – – 0 – 0
Mitochondria – – – – – – – – – – – – 0 – 0
Nuclear Periphery – – – – – – – – – – – – 0 – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – 0
Peroxisomes – – – – – – – – – – – – 0 – 0
SpindlePole – – – – – – – – – – – – 0 – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – 0
Cytoplasm – – – – – – – – – – – – 0 – 0
Nucleus – – – – – – – – – – – – 0 – 0
Vacuole – – – – – – – – – – – – 2.2 – 2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2247 3.9352 3.273 2.6206 2.2912 3.2145 3.9176 4.3192 3.7495 3.2184 3.667 3.8613 3.9542 3.5419 3.7642 3.4499 3.9465 3.3692
Actin 0.0229 0 0.0007 0 0.0118 0.0032 0.0159 0 0.0112 0.0335 0.0058 0.0003 0.0184 0 0.0002 0 0.0001 0.0001
Bud 0.0006 0 0.0001 0 0.0001 0.0001 0.0014 0 0.0008 0.0024 0.0017 0.0001 0.0002 0 0 0 0.0001 0
Bud Neck 0.0024 0.0001 0.0001 0.0001 0.0033 0.0011 0.0015 0.0001 0.0007 0.0007 0.0013 0.0007 0.0002 0.0001 0.0002 0 0.0006 0.0003
Bud Periphery 0.0011 0 0.0004 0 0.0003 0.0001 0.004 0 0.0008 0.0032 0.0061 0.0003 0.0004 0 0.0001 0 0.0002 0.0001
Bud Site 0.0029 0 0.0002 0 0.0005 0.0002 0.0032 0 0.0034 0.0044 0.0031 0.0001 0.0002 0 0 0 0.0002 0.0001
Cell Periphery 0.0004 0 0.0001 0 0.0001 0.0001 0.001 0 0.0001 0.0002 0.0007 0.0001 0.0001 0 0 0 0 0.0001
Cytoplasm 0.0028 0.0001 0.0001 0 0.0003 0.0001 0.0117 0 0.0017 0.0069 0.0033 0.0001 0.0002 0 0.0003 0.0001 0.0002 0.0004
Cytoplasmic Foci 0.0102 0 0.0002 0 0.0031 0.0002 0.0375 0 0.0194 0.0162 0.0344 0.0003 0.0008 0 0.0002 0 0 0.0008
Eisosomes 0.0002 0 0 0 0.0001 0 0.0003 0 0.0001 0.0002 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0042 0 0.0003 0 0.0005 0.0002 0.0142 0 0.0008 0.0051 0.0032 0.0009 0.0009 0 0.0015 0 0 0.0003
Endosome 0.0221 0 0.0026 0 0.0077 0.0008 0.0449 0 0.0056 0.0131 0.0593 0.0044 0.0022 0 0.0005 0 0.0001 0.0019
Golgi 0.0077 0 0.0006 0 0.0045 0.0006 0.0099 0 0.0069 0.0072 0.0184 0.0011 0.0016 0 0 0 0 0.0003
Lipid Particles 0.0307 0 0.0009 0 0.01 0.0011 0.0231 0 0.0033 0.0178 0.0211 0.004 0.0039 0 0.0001 0 0 0.0068
Mitochondria 0.006 0.0002 0.0051 0.0001 0.0015 0.0003 0.0222 0.0002 0.0121 0.0064 0.0367 0.0075 0.0012 0.0001 0.0005 0.0001 0.0007 0.0016
None 0.0048 0 0.0001 0 0.0008 0.0001 0.0061 0 0.0003 0.033 0.0009 0 0.0003 0 0.0127 0 0.0003 0.0004
Nuclear Periphery 0.0194 0.0063 0.0047 0.0012 0.0309 0.0016 0.0254 0.0006 0.0035 0.0062 0.0053 0.0012 0.0018 0.0005 0.0129 0.0011 0.0048 0.0004
Nucleolus 0.0616 0.0197 0.0399 0.0328 0.1812 0.0705 0.0403 0.0247 0.0125 0.0109 0.0727 0.0519 0.026 0.0282 0.0153 0.0156 0.1183 0.0407
Nucleus 0.7635 0.9727 0.9404 0.9649 0.7321 0.918 0.6877 0.9739 0.8975 0.7955 0.692 0.925 0.9392 0.9703 0.9483 0.9821 0.8672 0.9431
Peroxisomes 0.004 0 0.0002 0 0.0034 0.0003 0.009 0 0.0112 0.0053 0.0057 0.0001 0.0003 0 0 0 0 0.0003
Punctate Nuclear 0.0265 0.0004 0.0015 0.0007 0.0069 0.0012 0.0277 0.0002 0.0066 0.029 0.0029 0.0003 0.0011 0.0005 0.0057 0.0009 0.0066 0.0014
Vacuole 0.0041 0.0002 0.001 0 0.0005 0.0002 0.0083 0.0001 0.001 0.0019 0.0131 0.0009 0.0004 0.0002 0.0008 0 0.0002 0.0007
Vacuole Periphery 0.0016 0.0003 0.0008 0 0.0005 0.0001 0.0046 0.0001 0.0004 0.0007 0.0124 0.0005 0.0004 0.0001 0.0005 0 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.192 28.9116 24.4206 28.1134 20.5202 25.7903 34.0527 36.1016 30.4583 31.6452
Translational Efficiency 0.5513 0.6424 0.5447 0.4158 0.526 0.5694 0.539 0.4068 0.4763 0.4074

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2571 1283 428 1075 2060 1511 213 88 4631 2794 641 1163

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 683.34 797.02 1084.47 859.74 713.32 809.97 941.37 1064.75 696.68 804.02 1036.92 875.25
Standard Deviation 74.49 112.92 144.03 133.50 89.91 113.39 124.39 140.69 83.06 113.36 153.41 144.61
Intensity Change Log 2 — 0.222012 0.666315 0.331297 — 0.183319 0.400212 0.577893 — 0.202380 0.536539 0.462495

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000195 0.000708 0.001807 0.000987 0.000234 0.000447 0.000881 0.001854 0.000213 0.000567 0.001500 0.001053
Bud Neck 0.003141 0.006739 0.014669 0.013286 0.004624 0.009226 0.008876 0.015728 0.003801 0.008084 0.012744 0.013471
Bud Site 0.000949 0.001862 0.002294 0.006366 0.000882 0.001386 0.001779 0.002731 0.000919 0.001605 0.002123 0.006091
Cell Periphery 0.000080 0.000100 0.000103 0.000293 0.000082 0.000061 0.000125 0.000136 0.000081 0.000079 0.000110 0.000281
Cytoplasm 0.064224 0.005609 0.004034 0.045895 0.012263 0.004352 0.004443 0.005574 0.041110 0.004929 0.004170 0.042844
Cytoplasmic Foci 0.006216 0.001227 0.000116 0.005193 0.000871 0.001076 0.000580 0.003299 0.003839 0.001146 0.000270 0.005050
Eisosomes 0.000019 0.000027 0.000044 0.000044 0.000016 0.000022 0.000046 0.000044 0.000018 0.000025 0.000045 0.000044
Endoplasmic Reticulum 0.000777 0.004249 0.008857 0.006300 0.002028 0.003849 0.005294 0.007704 0.001334 0.004032 0.007673 0.006406
Endosome 0.000576 0.001228 0.000365 0.008552 0.000323 0.000814 0.001654 0.017600 0.000464 0.001004 0.000793 0.009237
Golgi 0.000163 0.000617 0.000149 0.003252 0.000164 0.000630 0.000627 0.004183 0.000163 0.000624 0.000308 0.003322
Lipid Particles 0.001280 0.000577 0.000317 0.000978 0.000449 0.001079 0.001827 0.000614 0.000910 0.000849 0.000819 0.000951
Mitochondria 0.002833 0.003662 0.001511 0.006108 0.001222 0.003650 0.001527 0.004692 0.002116 0.003656 0.001516 0.006001
Mitotic Spindle 0.002655 0.004145 0.002211 0.045117 0.002626 0.006207 0.009426 0.015925 0.002642 0.005260 0.004608 0.042908
None 0.016681 0.007456 0.001808 0.016910 0.011101 0.003771 0.015530 0.007176 0.014199 0.005463 0.006367 0.016173
Nuclear Periphery 0.002187 0.000582 0.000716 0.002922 0.000799 0.001462 0.000716 0.001707 0.001570 0.001058 0.000716 0.002830
Nuclear Periphery Foci 0.000627 0.001112 0.001185 0.002530 0.000984 0.000724 0.001101 0.003036 0.000786 0.000902 0.001157 0.002568
Nucleolus 0.025333 0.037530 0.008404 0.016091 0.035476 0.032014 0.024274 0.022673 0.029845 0.034547 0.013678 0.016589
Nucleus 0.870239* 0.919852* 0.947199* 0.798557* 0.925054* 0.925420* 0.916053* 0.848694* 0.894622* 0.922863* 0.936850* 0.802350*
Peroxisomes 0.000722 0.000913 0.000478 0.002222 0.000349 0.001447 0.000743 0.000738 0.000556 0.001202 0.000566 0.002110
Vacuole 0.000880 0.001008 0.003549 0.013971 0.000341 0.001067 0.003996 0.022521 0.000640 0.001040 0.003698 0.014618
Vacuole Periphery 0.000222 0.000796 0.000184 0.004428 0.000114 0.001296 0.000503 0.013369 0.000174 0.001067 0.000290 0.005104

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.97 -11.27 -9.14 -7.07 4.32 -5.49 -7.08 -9.78 -8.86 -4.72 -4.87 -13.87 -13.56 -11.67 2.23
Bud Neck -6.11 -11.61 -13.06 -9.48 0.60 -5.21 -3.73 -7.76 -5.42 -3.05 -7.86 -11.48 -13.70 -9.37 -1.06
Bud Site -1.72 -5.78 -7.06 -5.10 -4.14 -0.99 -3.23 -5.02 -4.53 -2.90 -1.97 -6.42 -7.41 -6.27 -4.66
Cell Periphery -1.38 -2.37 -6.55 -4.51 -4.24 1.97 -2.28 -3.89 -4.87 -1.30 0.06 -3.19 -6.78 -6.38 -4.41
Cytoplasm 20.79 21.58 8.42 -8.45 -9.29 5.83 6.22 2.05 -1.19 -1.41 21.11 21.63 3.79 -8.88 -9.44
Cytoplasmic Foci 7.61 9.72 4.87 -2.50 -4.78 -0.30 2.11 -0.07 0.07 -0.84 7.23 10.04 2.74 -2.77 -4.66
Eisosomes -3.13 -9.81 -13.29 -9.15 -1.42 -3.99 -6.94 -7.17 -6.28 -1.49 -4.52 -12.19 -15.91 -12.58 -1.37
Endoplasmic Reticulum -15.60 -15.30 -15.33 -8.34 -0.08 -8.25 -6.26 -6.38 -5.02 -2.62 -17.32 -15.75 -15.42 -9.82 -1.45
Endosome -1.67 1.63 -4.18 -3.38 -4.34 -2.50 -1.23 -1.42 -1.30 -1.15 -2.61 -0.98 -4.55 -3.94 -4.09
Golgi -2.84 -1.12 -2.76 -1.75 -2.68 -1.94 -1.06 -1.44 -0.93 -1.01 -3.13 -1.31 -2.96 -1.91 -2.60
Lipid Particles 4.22 5.88 2.89 -2.00 -4.15 -1.12 -0.87 -0.30 1.00 0.81 0.83 1.08 1.19 -0.20 -0.54
Mitochondria -0.74 1.71 -2.19 -0.94 -4.21 -2.97 -1.18 -3.92 0.09 -3.04 -2.22 1.02 -3.60 -1.12 -4.50
Mitotic Spindle -1.39 -0.06 -7.69 -6.96 -7.65 -2.11 -1.49 -2.79 -2.44 -1.84 -2.54 -1.40 -8.12 -7.26 -6.88
None 6.10 12.88 3.33 -2.04 -6.55 7.02 -0.21 1.93 -0.89 1.56 9.48 7.70 2.50 -3.66 -3.46
Nuclear Periphery 4.41 3.08 -2.22 -7.29 -5.90 -3.95 -0.46 -3.50 -2.26 -3.35 2.20 2.71 -4.32 -6.19 -6.51
Nuclear Periphery Foci -1.45 -1.10 -2.36 -1.04 0.10 1.29 -0.35 -1.34 -1.65 -0.86 -0.43 -1.11 -2.33 -2.05 -0.15
Nucleolus -3.12 6.46 6.24 8.22 -0.47 1.72 2.91 2.28 1.58 0.33 -1.24 7.18 8.52 9.15 0.80
Nucleus -8.68 -10.38 4.33 10.22 11.43 -0.34 1.12 3.35 3.41 2.55 -7.69 -6.13 7.98 11.85 11.03
Peroxisomes -0.84 1.08 -1.29 -0.70 -1.88 -2.27 -1.58 -2.61 1.47 0.21 -2.25 -0.52 -1.88 -0.02 -1.68
Vacuole -1.01 -7.78 -8.49 -8.34 -5.99 -4.20 -3.49 -3.43 -3.26 -2.33 -3.06 -7.95 -9.09 -8.83 -6.35
Vacuole Periphery -1.92 -0.44 -3.27 -2.11 -3.24 -1.97 -1.64 -1.31 -0.92 -1.20 -2.54 -1.89 -3.59 -1.87 -3.36
Standard name
Human Ortholog
Description Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; relocalizes to the cytosol in response to hypoxia; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Pdr1

Pdr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pdr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available