Standard name
Human Ortholog
Description Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion; activated in stochastic pulses of nuclear localization, shuttling between cytosol and nucleus depending on external glucose levels and its phosphorylation state
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.09
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0.06 0 0.07 0.05 0.06 0 0 0 0 0 0 0.15 0.18 0.31 0.23 0.2 0.15 0 0 0 0.08 0.12 0.16
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0.05 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.26 0.08 0.28 0.2 0.2 0.5 0.58 0.49 0.57 0.66 0.67 0.68 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Nucleus 0.75 0.77 0.77 0.8 0.76 0.66 0.59 0.69 0.7 0.65 0.66 0.64 0.78 0.64 0.44 0.59 0.52 0.66 0.71 0.75 0.68 0.6 0.38 0.26
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0 0 0.07 0 0.09 0.08 0.1 0.11 0 0 0 0 0 0 0.1 0.06 0.06 0.05 0.12 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.08
Vac/Vac Membrane 0.11 0.15 0.08 0 0.09 0.11 0 0 0 0 0 0.05 0.06 0.16 0.27 0.2 0.26 0.18 0.05 0.05 0.12 0.13 0.11 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1
Bud 0 0 1 0 0 0 5 0 0 3 0 3 0 0 0 1 0 0 4 5 5 5 18 19
Bud Neck 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 6
Bud Site 0 1 0 0 1 3 4 5 8 18 4 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 11 4 3 8 14 13 16 8 14 6 13 23 45 65 69 27 13 4 4 3 8 31 33
Endoplasmic Reticulum 0 1 0 0 0 1 0 0 1 0 0 0 0 1 1 13 12 5 0 0 0 0 1 1
Endosome 0 2 0 0 0 0 0 0 1 0 0 0 0 7 7 11 8 2 4 5 1 2 12 13
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 2 1 0 0 0 2
Mitochondria 36 15 36 9 30 118 189 185 158 209 145 199 3 5 1 14 1 5 0 2 0 0 2 3
Nucleus 105 143 99 35 113 157 193 259 196 206 142 187 119 155 94 179 71 59 107 165 103 62 100 56
Nuclear Periphery 0 0 0 0 1 0 4 3 2 7 2 0 0 0 0 0 0 1 0 0 0 0 1 1
Nucleolus 8 8 2 0 4 1 22 16 25 24 21 32 2 5 4 3 2 0 14 14 9 4 32 15
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 1 0 3 0 0 0 1 0 1 0 0 3 4 3 4 26 17
Vac/Vac Membrane 16 27 10 1 13 25 10 12 5 5 9 14 9 38 57 60 36 16 7 11 18 13 30 32
Unique Cell Count 140 185 128 44 149 238 325 377 279 316 215 291 152 244 212 304 137 89 151 219 152 104 267 214
Labelled Cell Count 173 208 152 48 170 320 441 497 404 489 329 454 156 259 229 354 158 102 151 219 152 104 267 214


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 6.2 4.9 5.1 5.1 4.7 4.1 4.6 4.6 4.2 4.3 4.3 6.9 6.4 6.0 7.8 9.3 10.9 6.4 6.7 7.3
Std Deviation (1e-4) 0.9 1.1 0.9 1.3 1.1 1.2 1.0 1.0 1.1 1.2 1.0 1.1 1.4 1.3 1.2 1.9 2.1 2.2 1.6 1.8 1.8
Intensity Change (Log2) – – – 0.07 0.06 -0.07 -0.25 -0.09 -0.1 -0.22 -0.21 -0.19 0.49 0.37 0.3 0.67 0.92 1.15 0.38 0.45 0.57


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.9 1.2 0 0.6 0 0.6 0 0.6 3.4 4.2 6.1 5.0 4.2 3.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 3.4 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.0 -1.6 0 0 0 0 0 0 0 -6.3 -7.6 -7.9 -7.0 -6.4 -4.2
Nucleus 0.3 -0.3 -2.3 -3.6 -1.9 -1.5 -2.5 -2.2 -2.6 0.2 -2.7 -6.0 -3.7 -4.3 -1.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0.3 0.8 -2.2 -2.2 0 0 -1.4 -1.2 -0.6 2.1 4.3 3.1 4.0 2.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.1932 3.6727 3.3362 3.3325 2.8486 3.6339 3.2475 3.1808 3.0165 2.7372 2.3142 3.0541 3.8954 4.723 4.0837 4.024 3.5321 4.2377
Actin 0.0005 0.0001 0.0001 0.0002 0.0004 0 0.0156 0.0009 0.0238 0.0002 0.0007 0.0002 0.0739 0 0.0287 0.0025 0.0001 0.0006
Bud 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.001 0.0003 0.0003 0.0001 0.0003 0.0001 0.0008 0 0.0006 0.0059 0.0001 0.0001
Bud Neck 0.0006 0.0003 0.0002 0.0002 0.0011 0.0018 0.0051 0.0011 0.0007 0.0004 0.001 0.0027 0.0009 0.0002 0.004 0.0026 0.0012 0.0032
Bud Periphery 0.0002 0 0 0 0.0001 0.0001 0.002 0.0002 0.0002 0 0.0002 0.0002 0.0014 0 0.0008 0.0128 0 0.0001
Bud Site 0.0006 0.0005 0.0004 0.0003 0.0004 0.0001 0.0033 0.0032 0.0092 0.0002 0.0002 0.0002 0.0083 0.0003 0.0022 0.0166 0.0006 0.0003
Cell Periphery 0.0001 0 0 0 0.0001 0 0.0007 0.0001 0.0001 0 0 0 0.0005 0 0.0002 0.0022 0 0
Cytoplasm 0.0289 0.0043 0.0179 0.0022 0.0104 0.0013 0.043 0.0486 0.0059 0.0651 0.0696 0.003 0.0479 0.0101 0.0059 0.1186 0.0112 0.0064
Cytoplasmic Foci 0.0047 0 0.0002 0 0.0004 0 0.0306 0.0174 0.0044 0.0003 0.0199 0.0018 0.0295 0.0002 0.0005 0.0039 0.0001 0.0004
Eisosomes 0 0 0 0 0 0 0.0004 0 0.0001 0 0 0 0.0007 0 0.0002 0.0003 0 0
Endoplasmic Reticulum 0.0088 0.0001 0.0002 0.0001 0.0029 0.0001 0.0058 0.0014 0.0003 0.0009 0.0021 0.0002 0.0122 0.0002 0.0015 0.0044 0.0005 0.0004
Endosome 0.021 0.0001 0.0002 0.0001 0.0028 0 0.0354 0.0036 0.0032 0.0005 0.0113 0.0059 0.058 0.0001 0.0033 0.0065 0.0007 0.0029
Golgi 0.0005 0 0 0 0.0001 0 0.0198 0.0007 0.0042 0 0.002 0.0046 0.0459 0 0.003 0.0017 0 0.0034
Lipid Particles 0.001 0 0 0 0.0004 0 0.0248 0.0001 0.0031 0 0.0006 0.0033 0.0913 0 0.0004 0.0031 0 0.0001
Mitochondria 0.0017 0.0002 0.0002 0.0003 0.0003 0.0001 0.0157 0.0007 0.0009 0.0004 0.0533 0.0009 0.1176 0.0001 0.0034 0.0124 0.0003 0.0171
None 0.0027 0.0002 0.0006 0.0002 0.0009 0 0.0113 0.0247 0.0048 0.0251 0.0072 0.0006 0.0036 0.0002 0.0012 0.0056 0.0006 0.001
Nuclear Periphery 0.0554 0.0046 0.0035 0.0036 0.0281 0.0009 0.0233 0.0127 0.004 0.0099 0.0138 0.0024 0.0177 0.0064 0.016 0.015 0.0109 0.0057
Nucleolus 0.0308 0.0093 0.0056 0.0032 0.0098 0.0183 0.0045 0.0144 0.0058 0.0023 0.007 0.0155 0.0018 0.0033 0.0037 0.013 0.0067 0.0115
Nucleus 0.7854 0.9636 0.9607 0.9813 0.9066 0.976 0.7161 0.8463 0.9028 0.8871 0.7769 0.9473 0.4536 0.9331 0.9136 0.7525 0.9586 0.9249
Peroxisomes 0.0001 0 0 0 0 0 0.0194 0.0006 0.0012 0 0.0012 0.0005 0.0222 0 0.0002 0.001 0 0.0001
Punctate Nuclear 0.0467 0.0163 0.0098 0.0079 0.0334 0.0007 0.0106 0.0193 0.0248 0.007 0.0306 0.01 0.0014 0.0453 0.0095 0.0044 0.0075 0.0212
Vacuole 0.007 0.0002 0.0002 0.0001 0.0013 0.0003 0.0082 0.0033 0.0003 0.0003 0.0016 0.0004 0.0058 0.0004 0.0006 0.0131 0.0006 0.0005
Vacuole Periphery 0.0029 0.0001 0.0001 0.0001 0.0003 0 0.0035 0.0005 0.0001 0.0001 0.0005 0.0001 0.0048 0.0001 0.0003 0.0019 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.7456 16.9279 21.2575 34.1888 16.0531 16.0142 21.0656 18.4388 27.4831 25.3236
Translational Efficiency 1.7035 1.422 1.3478 1.2489 2.0343 1.6255 1.0857 1.7475 1.5596 1.5849

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion; activated in stochastic pulses of nuclear localization, shuttling between cytosol and nucleus depending on external glucose levels and its phosphorylation state
Localization
Cell Percentages nucleus (93%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Mig1

Mig1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mig1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available