Standard name
Human Ortholog
Description ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0.06 0.05 0 0.05 0 0 0.12 0.12 0.13 0.06 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0.06 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.98 0.97 0.94 0.93 0.9 0.75 0.83 0.79 0.77 0.77 0.72 0.74 0.93 0.84 0.8 0.68 0.66 0.68 0.9 0.92 0.75 0.86 0.84 0.73
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.12 0.05 0.11 0 0.08 0.07 0 0.09 0.12 0.15 0.13 0 0 0 0.08 0.12 0.08 0.06 0 0.17 0 0.08 0.08
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0.15 0.1 0.16 0.12 0.08 0.09 0.08 0 0 0.05 0.12 0.18 0.17 0 0.05 0.06 0.08 0.06 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 0 0 0 0 0 3 0 0 1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 1 1 0 0 0 0 2 0 0 1 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 2 1 1 1 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 5 1 5 4 8 11 8 5 7 4 6 25 27 32 9 9 0 0 0 1 0 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 4 2 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 2 0 0 0 0 0 0 0 1 0 0 2 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 7 3 5 0 0 0 0 0 0
Mitochondria 0 1 0 2 0 3 4 0 2 9 7 9 1 0 0 2 2 2 0 0 0 1 0 1
Nucleus 42 58 146 65 99 88 200 142 124 120 110 106 141 174 176 168 95 98 53 77 122 88 145 158
Nuclear Periphery 0 0 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 7 7 8 3 9 16 4 15 18 23 19 2 4 7 19 17 12 3 1 28 1 14 17
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 3 3 1 4 18 24 28 19 13 13 11 4 5 10 29 26 24 0 4 9 8 10 32
Unique Cell Count 43 60 155 70 110 118 241 179 162 156 152 144 151 206 219 247 144 144 59 85 164 102 173 218
Labelled Cell Count 44 69 161 77 111 123 257 187 170 169 162 151 154 208 226 263 156 156 59 85 164 102 173 218


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 30.0 39.6 34.1 27.2 33.9 27.1 28.6 28.9 26.5 26.2 23.6 22.9 32.2 33.6 31.5 44.0 41.8 44.3 36.7 32.4 35.4
Std Deviation (1e-4) 6.7 9.9 10.0 7.1 13.8 6.4 7.2 8.2 7.7 7.8 7.6 6.9 9.8 13.4 12.4 17.4 14.7 18.3 13.8 10.4 11.9
Intensity Change (Log2) – – – -0.33 -0.01 -0.33 -0.26 -0.24 -0.36 -0.38 -0.53 -0.58 -0.09 -0.02 -0.11 0.37 0.29 0.37 0.11 -0.07 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.1 1.2 0.8 0 0.6 0 0.4 3.0 3.1 3.3 1.2 1.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus -0.4 -1.3 -4.6 -3.3 -3.9 -4.4 -4.3 -5.1 -4.9 -0.3 -2.9 -3.8 -6.2 -6.2 -5.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1.1 0.9 -1.2 1.7 2.3 3.1 2.7 0 0 -0.7 1.3 2.3 1.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 4.1 3.1 4.3 3.4 2.6 2.6 2.3 0 0 1.4 3.5 4.7 4.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 22.4155 28.451 23.5293 23.2515 22.3183 23.4284 20.6786 35.0579 29.7857 27.7065 26.541 27.3544 26.8813 34.6533 30.2471 32.0012 31.0414 31.1536
Actin 0.0039 0.0001 0.0022 0.0006 0.0011 0.0004 0.0635 0.0001 0.0017 0.0002 0.0001 0.0021 0.0204 0 0.0031 0 0 0.0002
Bud 0.0009 0 0.0002 0.0001 0.0001 0.0002 0.0016 0 0.0001 0.0003 0.0001 0.0007 0.0009 0 0.0001 0.0001 0 0
Bud Neck 0.0003 0 0.0002 0.0001 0.0003 0.001 0.0011 0 0 0.0001 0.0001 0.0006 0.0004 0 0.0001 0 0 0.0002
Bud Periphery 0.0005 0 0.0002 0.0001 0.0001 0.0004 0.0021 0 0.0001 0.0004 0.0001 0.0014 0.001 0 0.0001 0.0002 0 0.0001
Bud Site 0.0008 0 0.0004 0 0.0001 0.0002 0.002 0.0001 0.0001 0.0003 0.0001 0.0007 0.0022 0 0.0001 0.0001 0 0
Cell Periphery 0.0002 0 0.0001 0 0.0002 0.0003 0.0002 0 0 0.0001 0.0001 0.0004 0.0004 0 0.0001 0.0003 0 0.0001
Cytoplasm 0.0208 0.0116 0.0022 0.0011 0.038 0.0003 0.0156 0.0214 0.002 0.0003 0.0004 0.0018 0.0278 0.0001 0.0022 0.0001 0 0
Cytoplasmic Foci 0.0062 0.0001 0.0024 0 0.0011 0.0001 0.0027 0 0.0003 0 0.0001 0.0138 0.0305 0 0.0006 0 0 0.0011
Eisosomes 0.0001 0 0 0 0 0 0.0004 0 0.0002 0 0 0.0001 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0062 0 0.0022 0.0001 0.0015 0.0002 0.0076 0 0.0004 0 0 0.0005 0.0052 0 0.0003 0 0 0
Endosome 0.0089 0 0.0038 0.0001 0.0045 0.0006 0.0121 0 0.0027 0.0001 0 0.0054 0.0081 0 0.0025 0 0 0.0031
Golgi 0.001 0 0.0005 0 0.0004 0.0002 0.0051 0 0.0001 0 0 0.0012 0.0024 0 0.0078 0 0 0.0094
Lipid Particles 0.0028 0 0.0007 0 0.0003 0.0016 0.0029 0 0.0002 0 0 0.0045 0.0057 0 0.0009 0 0 0.0015
Mitochondria 0.0038 0.0002 0.0009 0.0016 0.0014 0.0022 0.0102 0.0017 0.0255 0.008 0.0021 0.0199 0.0087 0.0007 0.0015 0.0035 0.0009 0.0021
None 0.0029 0 0.0018 0.0001 0.0013 0.0001 0.0297 0.0001 0.0005 0.0001 0.0001 0.0019 0.0083 0 0.0002 0 0 0
Nuclear Periphery 0.0482 0.0065 0.0108 0.0399 0.0501 0.0106 0.061 0.0055 0.019 0.0031 0.0034 0.0128 0.0244 0.0043 0.0047 0.0039 0.0012 0.0007
Nucleolus 0.0433 0.0117 0.0277 0.0028 0.1263 0.0895 0.0341 0.0207 0.0085 0.027 0.1362 0.0791 0.0345 0.0139 0.0114 0.042 0.0709 0.0273
Nucleus 0.8268 0.9684 0.9268 0.9518 0.7668 0.8875 0.7327 0.9488 0.9353 0.9583 0.8525 0.8298 0.799 0.9806 0.9634 0.9467 0.926 0.953
Peroxisomes 0.0002 0 0.0002 0 0.0001 0.0001 0.001 0 0.0004 0 0 0.0017 0.0016 0 0.0001 0 0 0.0006
Punctate Nuclear 0.016 0.0011 0.0158 0.0008 0.002 0.0019 0.008 0.0007 0.0009 0.0005 0.0036 0.0142 0.0125 0 0.0004 0 0 0
Vacuole 0.0029 0.0001 0.0005 0.0002 0.0017 0.0011 0.0026 0.0001 0.0005 0.0003 0.0003 0.0021 0.0027 0.0001 0.0002 0.0009 0.0001 0.0002
Vacuole Periphery 0.0032 0.0001 0.0005 0.0007 0.0029 0.0015 0.0035 0.0007 0.0013 0.0007 0.0008 0.0054 0.0035 0.0002 0.0003 0.0021 0.0006 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 116.4101 110.1372 117.7824 111.7464 89.2664 97.7847 111.1549 113.293 125.7765 102.5871
Translational Efficiency 1.7701 1.8374 1.5853 1.8957 1.8367 2.2573 1.7628 1.6028 1.4414 1.4463

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
84 206 2078 1488 102 783 361 1521 186 989 2439 3009

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2267.50 3124.70 2849.37 2787.36 2515.44 2557.55 3034.04 2921.19 2403.47 2675.68 2876.70 2855.01
Standard Deviation 459.32 600.96 549.53 672.45 499.41 629.92 603.22 653.34 497.27 665.14 561.64 666.23
Intensity Change Log 2 — 0.462615 0.329540 0.297797 — 0.023952 0.270429 0.215745 — 0.248565 0.298755 0.255227

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000590 0.000568 0.000735 0.000887 0.001092 0.000766 0.001076 0.001112 0.000865 0.000725 0.000786 0.001001
Bud Neck 0.003689 0.004197 0.013017 0.018554 0.005563 0.005517 0.013000 0.015357 0.004716 0.005242 0.013015 0.016938
Bud Site 0.000397 0.000184 0.000586 0.002680 0.000338 0.000408 0.000739 0.001800 0.000365 0.000362 0.000608 0.002235
Cell Periphery 0.000029 0.000016 0.000054 0.000243 0.000026 0.000077 0.000046 0.000195 0.000028 0.000064 0.000053 0.000219
Cytoplasm 0.000066 0.000024 0.000199 0.001507 0.000048 0.000253 0.000234 0.001379 0.000056 0.000206 0.000205 0.001442
Cytoplasmic Foci 0.004091 0.000070 0.000088 0.001685 0.000269 0.000717 0.000414 0.001466 0.001995 0.000582 0.000136 0.001574
Eisosomes 0.000053 0.000064 0.000053 0.000041 0.000094 0.000056 0.000094 0.000032 0.000075 0.000058 0.000059 0.000037
Endoplasmic Reticulum 0.000440 0.000269 0.000575 0.000715 0.000508 0.000681 0.000408 0.000675 0.000477 0.000595 0.000550 0.000695
Endosome 0.000059 0.000005 0.000015 0.000995 0.000018 0.000434 0.000014 0.001571 0.000036 0.000344 0.000015 0.001286
Golgi 0.000134 0.000019 0.000038 0.000876 0.000071 0.000591 0.000066 0.001838 0.000099 0.000472 0.000042 0.001362
Lipid Particles 0.000139 0.000005 0.000005 0.000159 0.000018 0.000184 0.000012 0.000056 0.000073 0.000147 0.000006 0.000107
Mitochondria 0.001299 0.001389 0.002328 0.004689 0.002488 0.002321 0.003276 0.003670 0.001951 0.002127 0.002469 0.004174
Mitotic Spindle 0.010783 0.000052 0.000049 0.008923 0.000076 0.004035 0.000030 0.001397 0.004912 0.003206 0.000046 0.005119
None 0.000406 0.000321 0.000672 0.002272 0.000549 0.000601 0.000708 0.001343 0.000484 0.000542 0.000677 0.001803
Nuclear Periphery 0.000087 0.000072 0.000250 0.001223 0.000093 0.000330 0.000165 0.000984 0.000090 0.000277 0.000238 0.001102
Nuclear Periphery Foci 0.000327 0.000004 0.000007 0.000449 0.000106 0.000417 0.000007 0.000213 0.000205 0.000331 0.000007 0.000330
Nucleolus 0.019872 0.028386 0.021593 0.021503 0.079388 0.029437 0.032441 0.019124 0.052510 0.029218 0.023198 0.020300
Nucleus 0.953966* 0.960822* 0.958395* 0.928242* 0.904110* 0.950811* 0.943437* 0.945459* 0.926625* 0.952896* 0.956181* 0.936945*
Peroxisomes 0.002387 0.003071 0.000904 0.000813 0.004394 0.001205 0.003139 0.000503 0.003487 0.001594 0.001235 0.000656
Vacuole 0.001012 0.000248 0.000269 0.001898 0.000485 0.000648 0.000489 0.001183 0.000723 0.000565 0.000301 0.001536
Vacuole Periphery 0.000175 0.000215 0.000168 0.001649 0.000269 0.000510 0.000203 0.000642 0.000227 0.000448 0.000173 0.001140

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.37 -2.83 -4.74 -6.70 -3.81 2.74 -0.60 -0.06 -1.25 0.19 1.66 0.32 -1.13 -2.13 -1.41
Bud Neck -0.45 -10.90 -13.84 -14.93 -7.03 -0.28 -6.23 -11.50 -12.12 -2.72 -1.02 -13.84 -17.95 -18.51 -7.61
Bud Site 1.02 -0.76 -5.20 -7.20 -5.87 -1.07 -5.89 -4.36 -4.06 -2.80 0.13 -2.54 -6.91 -7.42 -6.33
Cell Periphery 1.44 -1.96 -5.12 -5.79 -4.68 -2.13 -4.23 -4.96 -2.87 -4.16 -1.82 -4.09 -7.16 -4.71 -6.20
Cytoplasm 2.01 -4.89 -5.60 -5.75 -5.19 -2.76 -2.31 -4.25 -3.64 -3.60 -2.48 -6.85 -6.89 -6.15 -6.24
Cytoplasmic Foci 1.05 1.04 0.81 -3.12 -3.00 -1.31 -2.02 -3.37 -1.61 -2.80 0.87 1.05 0.52 -2.22 -4.60
Eisosomes -1.12 -0.33 1.51 3.50 5.26 4.17 -0.61 6.83 6.22 12.60 2.78 2.09 6.19 6.47 11.64
Endoplasmic Reticulum 2.28 -1.77 -3.09 -9.18 -2.71 -1.28 0.65 -2.08 0.08 -4.00 -1.08 -1.68 -3.66 -0.94 -3.39
Endosome 1.09 0.91 -2.83 -3.31 -3.26 -1.61 -0.20 -2.92 -1.68 -2.92 -1.44 0.91 -3.91 -2.07 -4.06
Golgi 1.09 0.89 -1.52 -2.30 -2.20 -1.43 -0.54 -2.50 -1.34 -2.47 -1.19 0.96 -2.95 -1.42 -3.22
Lipid Particles 1.08 1.07 0.30 -3.42 -3.39 -2.08 0.00 -2.59 1.45 -2.66 -0.52 1.13 -0.07 0.70 -4.40
Mitochondria -0.52 -8.19 -5.47 -5.34 -3.38 0.36 -3.74 -2.18 -2.33 -0.54 -1.00 -4.91 -5.15 -4.48 -3.53
Mitotic Spindle 1.02 1.00 0.15 -4.98 -4.81 -2.23 0.07 -3.71 0.82 -3.73 0.33 0.98 -0.19 -1.48 -5.63
None 1.63 -3.48 -5.63 -5.89 -4.50 -0.81 -2.48 -4.37 -3.98 -3.33 -1.13 -3.22 -7.06 -6.59 -5.39
Nuclear Periphery 0.71 -4.29 -3.96 -4.08 -3.37 -3.05 -3.66 -8.68 -5.67 -7.85 -2.88 -6.48 -7.13 -5.52 -6.06
Nuclear Periphery Foci 1.04 1.03 0.03 -3.41 -3.36 -1.69 1.05 -1.07 1.07 -2.98 -0.29 1.25 -0.33 -0.00 -4.39
Nucleolus -1.19 -0.66 -0.68 0.88 -0.06 3.47 3.06 4.15 2.63 3.32 2.69 3.36 3.71 2.61 1.37
Nucleus -0.56 -0.23 1.70 4.90 6.97 -2.96 -2.09 -2.09 2.42 0.12 -2.28 -2.35 -0.30 4.94 6.91
Peroxisomes -0.25 1.56 1.75 1.41 1.38 4.04 0.70 4.92 4.06 5.97 2.69 3.37 4.53 2.50 5.45
Vacuole 1.07 0.98 -0.30 -2.92 -2.73 -1.09 -1.78 -2.70 -2.02 -2.27 0.63 1.05 -1.20 -3.04 -3.88
Vacuole Periphery -0.19 0.32 -2.09 -2.08 -2.17 -1.96 1.28 -2.46 -0.41 -2.95 -2.08 1.42 -2.67 -1.66 -2.93
Standard name
Human Ortholog
Description ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-5

Rpt6

Rpt6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpt6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available