Standard name | |
---|---|
Human Ortholog | |
Description | Ubiquitin-conjugating enzyme (E2); involved in postreplication repair as a heterodimer with Rad18p, regulation of K63 polyubiquitination in response to oxidative stress, DSBR and checkpoint control as a heterodimer with Bre1p, ubiquitin-mediated N-end rule protein degradation as a heterodimer with Ubr1p, ERAD with Ubr1p in the absence of canonical ER membrane ligases, and Rpn4p turnover as part of proteasome homeostasis, in complex with Ubr2p and Mub1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 2 | – | 0 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 2 | 4 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 2 | 6 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 3 | 6 | 9 | 19 | 20 | 32 | – | 21 | 11 | 26 | 22 | 5 | 35 | 53 | 10 | 9 | 23 | 0 | 0 | 0 | 1 | 0 | 1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 5 | 6 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 1 | 1 | 0 | 1 | – | 4 | 6 | 9 | 12 | 1 | 0 | 2 | 0 | 8 | 1 | 1 | 1 | 1 | 0 | 1 | 1 |
Golgi | 0 | 0 | 1 | 0 | 8 | 0 | 22 | – | 1 | 9 | 1 | 0 | 0 | 4 | 2 | 24 | 29 | 21 | 1 | 0 | 0 | 0 | 1 | 2 |
Mitochondria | 0 | 1 | 0 | 1 | 0 | 25 | 5 | – | 27 | 21 | 50 | 42 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 0 |
Nucleus | 152 | 81 | 119 | 231 | 253 | 199 | 362 | – | 248 | 237 | 274 | 313 | 214 | 271 | 201 | 160 | 155 | 141 | 177 | 97 | 122 | 145 | 265 | 193 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 1 | 1 | 1 | – | 4 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 27 | 10 | 4 | 5 | 5 | 23 | 13 | – | 23 | 32 | 58 | 65 | 16 | 8 | 14 | 6 | 3 | 9 | 9 | 3 | 4 | 9 | 37 | 31 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 1 | 3 | – | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
Vac/Vac Membrane | 10 | 4 | 6 | 9 | 44 | 41 | 72 | – | 41 | 46 | 61 | 76 | 10 | 23 | 33 | 59 | 65 | 103 | 3 | 2 | 3 | 7 | 27 | 52 |
Unique Cell Count | 182 | 89 | 131 | 251 | 322 | 280 | 477 | 331 | 336 | 427 | 476 | 239 | 335 | 299 | 243 | 245 | 275 | 201 | 107 | 137 | 171 | 343 | 291 | |
Labelled Cell Count | 194 | 99 | 136 | 258 | 331 | 311 | 513 | 371 | 367 | 489 | 540 | 246 | 341 | 311 | 267 | 278 | 306 | 201 | 107 | 137 | 171 | 343 | 291 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 15.2 | 17.5 | 13.8 | 14.5 | 17.1 | 10.0 | 14.6 | – | 12.3 | 12.7 | 11.4 | 11.7 | 19.3 | 18.6 | 19.2 | 28.3 | 26.8 | 26.5 | 14.2 | 15.7 | 15.5 |
Std Deviation (1e-4) | 6.2 | 8.3 | 5.2 | 5.4 | 6.1 | 4.4 | 6.4 | – | 5.4 | 5.2 | 4.9 | 5.0 | 6.7 | 7.5 | 7.8 | 8.9 | 8.7 | 8.9 | 6.2 | 7.0 | 7.5 |
Intensity Change (Log2) | – | – | – | 0.08 | 0.32 | -0.46 | 0.09 | – | -0.16 | -0.12 | -0.27 | -0.24 | 0.49 | 0.43 | 0.48 | 1.04 | 0.96 | 0.95 | 0.04 | 0.19 | 0.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.5 | 0.6 | 1.0 | 0.9 | – | 0.7 | 0 | 0.6 | 0 | 0 | 2.0 | 3.6 | -0.2 | -0.4 | 1.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 3.8 | 2.9 |
Mitochondria | 0 | 0 | 3.5 | 0 | – | 3.4 | 2.9 | 4.1 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.4 | -3.1 | -4.5 | -3.7 | – | -3.8 | -4.6 | -5.9 | -5.6 | -0.4 | -2.6 | -5.2 | -5.3 | -5.7 | -7.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 2.0 | 0 | – | 1.6 | 2.4 | 3.4 | 3.4 | 1.5 | 0 | 0.8 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.5 | 2.8 | 3.0 | 3.2 | – | 2.5 | 2.8 | 3.0 | 3.4 | -0.2 | 0.9 | 2.1 | 4.8 | 5.2 | 7.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.5907 | 4.0182 | 2.9824 | 2.8019 | 2.4393 | 2.9634 | 5.3508 | 6.0434 | 5.105 | 4.4721 | 5.0087 | 5.2993 | 6.3035 | 7.6322 | 6.4081 | 6.6508 | 6.1973 | 6.2411 |
Actin | 0.0339 | 0 | 0.0065 | 0 | 0.0495 | 0.001 | 0.0388 | 0.0007 | 0.0133 | 0.0066 | 0.0072 | 0.0023 | 0.0028 | 0.0025 | 0.0108 | 0.0115 | 0 | 0.0004 |
Bud | 0.0009 | 0 | 0.0001 | 0 | 0.0006 | 0 | 0.0015 | 0.0001 | 0.001 | 0.0001 | 0.0008 | 0.0001 | 0.0007 | 0.0001 | 0.0003 | 0.0003 | 0 | 0.0002 |
Bud Neck | 0.0067 | 0 | 0.0004 | 0 | 0.0011 | 0.0004 | 0.0052 | 0.0003 | 0.0022 | 0.0003 | 0.0004 | 0.0007 | 0.0018 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0006 |
Bud Periphery | 0.0023 | 0 | 0.0002 | 0 | 0.0007 | 0.0001 | 0.0037 | 0.0001 | 0.0029 | 0.0001 | 0.0015 | 0.0002 | 0.0023 | 0.0001 | 0.0008 | 0.0005 | 0 | 0.0003 |
Bud Site | 0.0049 | 0 | 0.0003 | 0 | 0.0101 | 0.0001 | 0.0083 | 0.0012 | 0.0108 | 0.0003 | 0.0048 | 0.0002 | 0.0025 | 0.001 | 0.0058 | 0.0003 | 0 | 0.0005 |
Cell Periphery | 0.0014 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0019 | 0 | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0013 | 0.0001 | 0.0003 | 0 | 0 | 0.0001 |
Cytoplasm | 0.0069 | 0 | 0.0006 | 0 | 0.0003 | 0.0006 | 0.0028 | 0.0008 | 0.0022 | 0.0018 | 0.0011 | 0.0003 | 0.0043 | 0.007 | 0.0033 | 0 | 0 | 0.0004 |
Cytoplasmic Foci | 0.01 | 0 | 0.0017 | 0 | 0.0146 | 0.0011 | 0.021 | 0.0021 | 0.0078 | 0.0116 | 0.007 | 0.0006 | 0.0155 | 0.005 | 0.0127 | 0.0015 | 0 | 0.0006 |
Eisosomes | 0.0009 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0053 | 0 | 0.0008 | 0 | 0.0008 | 0.0008 | 0.0045 | 0.0001 | 0.003 | 0.0016 | 0.0046 | 0.001 | 0.0033 | 0.0003 | 0.0008 | 0 | 0 | 0.0002 |
Endosome | 0.0211 | 0 | 0.0095 | 0 | 0.0095 | 0.0054 | 0.0256 | 0.0028 | 0.0098 | 0.0087 | 0.042 | 0.0041 | 0.0107 | 0.0013 | 0.002 | 0.0015 | 0 | 0.0031 |
Golgi | 0.0063 | 0 | 0.0044 | 0 | 0.0142 | 0.0018 | 0.0141 | 0.0015 | 0.0037 | 0.0025 | 0.0184 | 0.003 | 0.0038 | 0.0018 | 0.0012 | 0.003 | 0 | 0.0004 |
Lipid Particles | 0.0141 | 0 | 0.0039 | 0 | 0.0127 | 0.0017 | 0.0278 | 0.0018 | 0.0082 | 0.01 | 0.0291 | 0.0044 | 0.0109 | 0.0003 | 0.0025 | 0.0008 | 0 | 0.0008 |
Mitochondria | 0.0078 | 0 | 0.0057 | 0 | 0.003 | 0.0021 | 0.0398 | 0.0005 | 0.007 | 0.0006 | 0.0908 | 0.0021 | 0.0134 | 0.0004 | 0.0087 | 0.047 | 0.0001 | 0.0076 |
None | 0.0039 | 0 | 0.0009 | 0 | 0.0003 | 0.0007 | 0.006 | 0.0002 | 0.0008 | 0.0016 | 0.0015 | 0.0001 | 0.0013 | 0 | 0.0073 | 0.0001 | 0 | 0.0001 |
Nuclear Periphery | 0.0269 | 0.0009 | 0.0091 | 0.0003 | 0.0009 | 0.0144 | 0.0268 | 0.0021 | 0.0027 | 0.0049 | 0.0199 | 0.0044 | 0.0404 | 0.0015 | 0.007 | 0.0001 | 0.0004 | 0.001 |
Nucleolus | 0.0118 | 0.0039 | 0.0051 | 0.0024 | 0.0296 | 0.0161 | 0.0113 | 0.0055 | 0.0075 | 0.0049 | 0.0119 | 0.011 | 0.0259 | 0.0038 | 0.0036 | 0.0014 | 0.0258 | 0.0129 |
Nucleus | 0.8067 | 0.9947 | 0.946 | 0.9972 | 0.8414 | 0.9512 | 0.7343 | 0.9759 | 0.9 | 0.9272 | 0.7416 | 0.9631 | 0.8236 | 0.9616 | 0.9249 | 0.9216 | 0.9731 | 0.9526 |
Peroxisomes | 0.0076 | 0 | 0.0007 | 0 | 0.0069 | 0.0002 | 0.0101 | 0.0005 | 0.0082 | 0.0015 | 0.004 | 0.0002 | 0.0098 | 0.0051 | 0.0027 | 0.0101 | 0 | 0.0005 |
Punctate Nuclear | 0.0136 | 0.0003 | 0.0018 | 0 | 0.002 | 0.0014 | 0.0062 | 0.0035 | 0.0033 | 0.0153 | 0.0028 | 0.001 | 0.0161 | 0.0009 | 0.0035 | 0.0001 | 0.0002 | 0.0112 |
Vacuole | 0.0044 | 0 | 0.0011 | 0 | 0.0011 | 0.0003 | 0.0057 | 0.0002 | 0.0036 | 0.0002 | 0.0056 | 0.0005 | 0.006 | 0.0063 | 0.0008 | 0.0001 | 0 | 0.0053 |
Vacuole Periphery | 0.0027 | 0 | 0.0009 | 0 | 0.0003 | 0.0006 | 0.0041 | 0.0001 | 0.0017 | 0.0002 | 0.0043 | 0.0005 | 0.0036 | 0.0008 | 0.0004 | 0.0001 | 0 | 0.0012 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 70.1587 | 87.4245 | 63.4625 | 58.9248 | 79.9379 | 80.744 | 58.1292 | 75.2241 | 72.4397 | 69.1743 |
Translational Efficiency | 1.7682 | 1.6159 | 1.8831 | 2.5707 | 1.5853 | 1.6079 | 2.3315 | 2.0777 | 1.8194 | 1.8027 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1292 | 233 | 249 | 83 | 1187 | 689 | 1798 | 999 | 2479 | 922 | 2047 | 1082 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 812.26 | 974.10 | 1256.13 | 2090.25 | 802.43 | 944.11 | 1339.06 | 1919.66 | 807.55 | 951.69 | 1328.97 | 1932.75 |
Standard Deviation | 110.88 | 135.11 | 162.59 | 445.02 | 108.76 | 137.40 | 214.04 | 403.00 | 109.98 | 137.44 | 210.22 | 408.91 |
Intensity Change Log 2 | — | 0.262128 | 0.628972 | 1.363662 | — | 0.234579 | 0.738773 | 1.258403 | — | 0.248503 | 0.684583 | 1.312313 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000275 | 0.000706 | 0.001040 | 0.000761 | 0.000258 | 0.000512 | 0.000541 | 0.001499 | 0.000267 | 0.000561 | 0.000602 | 0.001443 |
Bud Neck | 0.003783 | 0.014069 | 0.013970 | 0.014355 | 0.003276 | 0.007717 | 0.019574 | 0.035345 | 0.003540 | 0.009323 | 0.018892 | 0.033734 |
Bud Site | 0.001357 | 0.004157 | 0.001556 | 0.005792 | 0.001068 | 0.002916 | 0.001137 | 0.012897 | 0.001218 | 0.003229 | 0.001188 | 0.012352 |
Cell Periphery | 0.000126 | 0.000184 | 0.000090 | 0.000315 | 0.000055 | 0.000096 | 0.000071 | 0.000242 | 0.000092 | 0.000118 | 0.000073 | 0.000248 |
Cytoplasm | 0.001767 | 0.004833 | 0.000977 | 0.009722 | 0.002443 | 0.004407 | 0.001382 | 0.016641 | 0.002090 | 0.004514 | 0.001333 | 0.016110 |
Cytoplasmic Foci | 0.000407 | 0.007290 | 0.000056 | 0.001548 | 0.000215 | 0.002888 | 0.000019 | 0.003856 | 0.000315 | 0.004000 | 0.000023 | 0.003679 |
Eisosomes | 0.000021 | 0.000025 | 0.000049 | 0.000046 | 0.000014 | 0.000022 | 0.000035 | 0.000034 | 0.000017 | 0.000023 | 0.000037 | 0.000035 |
Endoplasmic Reticulum | 0.004268 | 0.006263 | 0.003856 | 0.000921 | 0.003785 | 0.005970 | 0.001573 | 0.001097 | 0.004037 | 0.006044 | 0.001851 | 0.001084 |
Endosome | 0.000362 | 0.006201 | 0.000114 | 0.011058 | 0.000183 | 0.003412 | 0.000070 | 0.006714 | 0.000276 | 0.004117 | 0.000075 | 0.007047 |
Golgi | 0.000230 | 0.005741 | 0.000068 | 0.003296 | 0.000149 | 0.003616 | 0.000048 | 0.013039 | 0.000191 | 0.004153 | 0.000050 | 0.012292 |
Lipid Particles | 0.000442 | 0.000866 | 0.000081 | 0.000079 | 0.000151 | 0.000674 | 0.000017 | 0.000701 | 0.000302 | 0.000723 | 0.000024 | 0.000653 |
Mitochondria | 0.002292 | 0.003283 | 0.001281 | 0.001862 | 0.001212 | 0.007844 | 0.001257 | 0.003724 | 0.001775 | 0.006691 | 0.001260 | 0.003581 |
Mitotic Spindle | 0.001866 | 0.009194 | 0.000159 | 0.001917 | 0.002508 | 0.005604 | 0.000782 | 0.052815 | 0.002174 | 0.006511 | 0.000706 | 0.048910 |
None | 0.003061 | 0.006172 | 0.001171 | 0.004425 | 0.002339 | 0.001687 | 0.000949 | 0.006086 | 0.002715 | 0.002821 | 0.000976 | 0.005959 |
Nuclear Periphery | 0.000314 | 0.000339 | 0.000243 | 0.000498 | 0.000273 | 0.001753 | 0.000183 | 0.001456 | 0.000294 | 0.001396 | 0.000190 | 0.001383 |
Nuclear Periphery Foci | 0.000236 | 0.002494 | 0.000068 | 0.000091 | 0.000232 | 0.000847 | 0.000045 | 0.001432 | 0.000234 | 0.001263 | 0.000048 | 0.001329 |
Nucleolus | 0.005867 | 0.003455 | 0.003128 | 0.000838 | 0.004697 | 0.004625 | 0.003012 | 0.001893 | 0.005307 | 0.004330 | 0.003026 | 0.001812 |
Nucleus | 0.971645* | 0.915046* | 0.970328* | 0.907376* | 0.975995* | 0.938974* | 0.968533* | 0.827302* | 0.973728* | 0.932927* | 0.968751* | 0.833445* |
Peroxisomes | 0.000489 | 0.000796 | 0.000317 | 0.000362 | 0.000457 | 0.001588 | 0.000109 | 0.000389 | 0.000473 | 0.001388 | 0.000135 | 0.000387 |
Vacuole | 0.000728 | 0.005535 | 0.001348 | 0.026791 | 0.000515 | 0.002797 | 0.000539 | 0.008781 | 0.000626 | 0.003489 | 0.000637 | 0.010163 |
Vacuole Periphery | 0.000465 | 0.003350 | 0.000098 | 0.007945 | 0.000175 | 0.002051 | 0.000124 | 0.004055 | 0.000326 | 0.002379 | 0.000121 | 0.004353 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.76 | -8.66 | -4.56 | -1.98 | -0.14 | -3.24 | -8.06 | -3.47 | -2.04 | -1.85 | -4.14 | -10.45 | -3.81 | -2.07 | -1.72 |
Bud Neck | -2.84 | -8.31 | -5.57 | -1.53 | -1.78 | -3.19 | -16.47 | -14.96 | -10.97 | -5.23 | -4.21 | -17.92 | -15.57 | -10.38 | -5.60 |
Bud Site | -2.28 | -0.85 | -3.18 | -0.80 | -3.03 | -1.80 | -0.37 | -5.50 | -3.29 | -5.91 | -2.53 | -0.35 | -5.88 | -3.31 | -6.17 |
Cell Periphery | -0.78 | 1.12 | -3.71 | -1.94 | -4.38 | -2.43 | -1.77 | -8.69 | -5.23 | -7.89 | -1.04 | 1.40 | -6.50 | -4.11 | -8.68 |
Cytoplasm | -1.83 | 1.67 | -2.93 | -1.35 | -3.32 | -1.42 | 1.71 | -6.29 | -4.11 | -7.18 | -2.19 | 1.77 | -6.97 | -4.43 | -7.55 |
Cytoplasmic Foci | -2.37 | 1.46 | -0.59 | 2.29 | -1.94 | -3.07 | 2.74 | -3.09 | 1.82 | -4.22 | -3.74 | 2.46 | -2.76 | 2.78 | -4.36 |
Eisosomes | -1.04 | -8.12 | -4.93 | -3.99 | -1.20 | -3.52 | -14.43 | -12.13 | -6.51 | -2.02 | -2.60 | -13.55 | -11.41 | -7.29 | -1.99 |
Endoplasmic Reticulum | -2.08 | 0.43 | 8.82 | 5.08 | 6.49 | -4.21 | 11.12 | 12.51 | 9.52 | 3.08 | -4.39 | 12.17 | 15.73 | 10.84 | 5.81 |
Endosome | -2.62 | 2.10 | -0.90 | 0.99 | -0.99 | -3.24 | 2.79 | -3.42 | 1.25 | -3.65 | -4.11 | 3.29 | -3.34 | 1.91 | -3.75 |
Golgi | -2.56 | 1.16 | -0.90 | 2.09 | -1.12 | -2.74 | 0.92 | -2.45 | -0.04 | -2.53 | -3.63 | 1.63 | -2.46 | 0.38 | -2.58 |
Lipid Particles | -0.74 | 1.62 | 1.39 | 1.43 | -1.06 | -1.61 | 3.57 | -1.17 | 1.11 | -2.51 | -1.39 | 2.43 | 0.15 | 1.50 | -2.51 |
Mitochondria | -0.64 | 1.09 | -0.91 | 0.08 | -3.77 | -2.76 | -0.43 | -3.63 | 2.03 | -5.90 | -2.65 | 0.76 | -2.45 | 2.02 | -6.43 |
Mitotic Spindle | -1.37 | 2.17 | -1.12 | 0.59 | -1.89 | -1.43 | 1.32 | -6.29 | -5.45 | -6.75 | -2.06 | 1.83 | -6.44 | -5.20 | -6.83 |
None | -1.12 | 2.89 | -2.06 | -0.11 | -3.31 | 1.18 | 2.95 | -5.52 | -6.97 | -8.76 | -0.13 | 4.48 | -5.56 | -4.09 | -9.22 |
Nuclear Periphery | -0.24 | -0.23 | -3.20 | -3.09 | -3.16 | -1.15 | 0.73 | -6.67 | 0.09 | -7.41 | -1.14 | 1.14 | -7.09 | -0.21 | -7.69 |
Nuclear Periphery Foci | -1.16 | 1.98 | 1.73 | 1.23 | -0.51 | -1.52 | 1.95 | -1.59 | 0.79 | -3.20 | -1.79 | 3.05 | -1.65 | 1.33 | -3.20 |
Nucleolus | 2.00 | 0.38 | 5.47 | 2.02 | 2.71 | 0.03 | 1.63 | 3.68 | 1.69 | 3.53 | 0.83 | 2.75 | 5.98 | 2.00 | 4.25 |
Nucleus | 3.78 | 1.54 | 3.20 | -0.41 | 2.79 | 4.79 | 3.93 | 12.97 | 6.06 | 11.81 | 5.93 | 3.92 | 13.28 | 5.59 | 12.12 |
Peroxisomes | -0.75 | 0.70 | 1.54 | 1.82 | 2.23 | -1.43 | 1.14 | 0.89 | 1.96 | -1.66 | -1.58 | 1.89 | 1.60 | 2.22 | -1.07 |
Vacuole | -2.94 | -3.43 | -1.87 | -1.00 | -1.72 | -3.62 | -0.22 | -6.45 | -3.65 | -6.43 | -4.57 | -0.40 | -6.62 | -3.52 | -6.59 |
Vacuole Periphery | -1.45 | 1.73 | -0.80 | 0.48 | -1.00 | -2.11 | 0.72 | -2.47 | 0.85 | -2.63 | -2.45 | 1.77 | -2.22 | 1.18 | -2.82 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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