Standard name
Human Ortholog
Description Ubiquitin-conjugating enzyme (E2); involved in postreplication repair as a heterodimer with Rad18p, regulation of K63 polyubiquitination in response to oxidative stress, DSBR and checkpoint control as a heterodimer with Bre1p, ubiquitin-mediated N-end rule protein degradation as a heterodimer with Ubr1p, ERAD with Ubr1p in the absence of canonical ER membrane ligases, and Rpn4p turnover as part of proteasome homeostasis, in complex with Ubr2p and Mub1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0 0.06 0.07 0.07 – 0.06 0 0.06 0.05 0 0.1 0.18 0 0 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0.05 – 0 0 0 0 0 0 0 0.1 0.12 0.08 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.09 0 – 0.08 0.06 0.12 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.84 0.91 0.91 0.92 0.79 0.71 0.76 – 0.75 0.71 0.64 0.66 0.9 0.81 0.67 0.66 0.63 0.51 0.88 0.91 0.89 0.85 0.77 0.66
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.15 0.11 0 0 0 0.08 0 – 0.07 0.1 0.14 0.14 0.07 0 0.05 0 0 0 0.05 0 0 0.06 0.11 0.11
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0.05 0 0.14 0.15 0.15 – 0.12 0.14 0.14 0.16 0 0.07 0.11 0.24 0.27 0.37 0 0 0 0.05 0.08 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0
Bud 0 0 0 0 0 0 2 – 0 2 2 2 0 0 0 0 0 0 3 0 1 2 4 3
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 0 – 1 2 6 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 5 3 6 9 19 20 32 – 21 11 26 22 5 35 53 10 9 23 0 0 0 1 0 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 5 6 6 0 0 0 0 0 0
Endosome 0 0 0 1 1 0 1 – 4 6 9 12 1 0 2 0 8 1 1 1 1 0 1 1
Golgi 0 0 1 0 8 0 22 – 1 9 1 0 0 4 2 24 29 21 1 0 0 0 1 2
Mitochondria 0 1 0 1 0 25 5 – 27 21 50 42 0 0 0 1 1 1 0 0 2 0 0 0
Nucleus 152 81 119 231 253 199 362 – 248 237 274 313 214 271 201 160 155 141 177 97 122 145 265 193
Nuclear Periphery 0 0 0 1 1 1 1 – 4 0 1 2 0 0 0 1 0 0 0 0 0 0 1 0
Nucleolus 27 10 4 5 5 23 13 – 23 32 58 65 16 8 14 6 3 9 9 3 4 9 37 31
Peroxisomes 0 0 0 0 0 0 0 – 0 1 0 0 0 0 3 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 1 3 – 1 0 0 1 0 0 2 0 2 0 0 0 0 0 1 3
Vac/Vac Membrane 10 4 6 9 44 41 72 – 41 46 61 76 10 23 33 59 65 103 3 2 3 7 27 52
Unique Cell Count 182 89 131 251 322 280 477 331 336 427 476 239 335 299 243 245 275 201 107 137 171 343 291
Labelled Cell Count 194 99 136 258 331 311 513 371 367 489 540 246 341 311 267 278 306 201 107 137 171 343 291


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.2 17.5 13.8 14.5 17.1 10.0 14.6 – 12.3 12.7 11.4 11.7 19.3 18.6 19.2 28.3 26.8 26.5 14.2 15.7 15.5
Std Deviation (1e-4) 6.2 8.3 5.2 5.4 6.1 4.4 6.4 – 5.4 5.2 4.9 5.0 6.7 7.5 7.8 8.9 8.7 8.9 6.2 7.0 7.5
Intensity Change (Log2) – – – 0.08 0.32 -0.46 0.09 – -0.16 -0.12 -0.27 -0.24 0.49 0.43 0.48 1.04 0.96 0.95 0.04 0.19 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 0.6 1.0 0.9 – 0.7 0 0.6 0 0 2.0 3.6 -0.2 -0.4 1.4
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 3.4 3.8 2.9
Mitochondria 0 0 3.5 0 – 3.4 2.9 4.1 3.5 0 0 0 0 0 0
Nucleus 0.4 -3.1 -4.5 -3.7 – -3.8 -4.6 -5.9 -5.6 -0.4 -2.6 -5.2 -5.3 -5.7 -7.8
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 2.0 0 – 1.6 2.4 3.4 3.4 1.5 0 0.8 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole -0.5 2.8 3.0 3.2 – 2.5 2.8 3.0 3.4 -0.2 0.9 2.1 4.8 5.2 7.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.5907 4.0182 2.9824 2.8019 2.4393 2.9634 5.3508 6.0434 5.105 4.4721 5.0087 5.2993 6.3035 7.6322 6.4081 6.6508 6.1973 6.2411
Actin 0.0339 0 0.0065 0 0.0495 0.001 0.0388 0.0007 0.0133 0.0066 0.0072 0.0023 0.0028 0.0025 0.0108 0.0115 0 0.0004
Bud 0.0009 0 0.0001 0 0.0006 0 0.0015 0.0001 0.001 0.0001 0.0008 0.0001 0.0007 0.0001 0.0003 0.0003 0 0.0002
Bud Neck 0.0067 0 0.0004 0 0.0011 0.0004 0.0052 0.0003 0.0022 0.0003 0.0004 0.0007 0.0018 0.0001 0.0004 0.0001 0.0001 0.0006
Bud Periphery 0.0023 0 0.0002 0 0.0007 0.0001 0.0037 0.0001 0.0029 0.0001 0.0015 0.0002 0.0023 0.0001 0.0008 0.0005 0 0.0003
Bud Site 0.0049 0 0.0003 0 0.0101 0.0001 0.0083 0.0012 0.0108 0.0003 0.0048 0.0002 0.0025 0.001 0.0058 0.0003 0 0.0005
Cell Periphery 0.0014 0 0.0001 0 0.0001 0 0.0019 0 0.0004 0 0.0003 0.0001 0.0013 0.0001 0.0003 0 0 0.0001
Cytoplasm 0.0069 0 0.0006 0 0.0003 0.0006 0.0028 0.0008 0.0022 0.0018 0.0011 0.0003 0.0043 0.007 0.0033 0 0 0.0004
Cytoplasmic Foci 0.01 0 0.0017 0 0.0146 0.0011 0.021 0.0021 0.0078 0.0116 0.007 0.0006 0.0155 0.005 0.0127 0.0015 0 0.0006
Eisosomes 0.0009 0 0.0001 0 0.0002 0 0.0008 0 0.0001 0 0.0002 0 0.0001 0 0.0002 0.0001 0 0
Endoplasmic Reticulum 0.0053 0 0.0008 0 0.0008 0.0008 0.0045 0.0001 0.003 0.0016 0.0046 0.001 0.0033 0.0003 0.0008 0 0 0.0002
Endosome 0.0211 0 0.0095 0 0.0095 0.0054 0.0256 0.0028 0.0098 0.0087 0.042 0.0041 0.0107 0.0013 0.002 0.0015 0 0.0031
Golgi 0.0063 0 0.0044 0 0.0142 0.0018 0.0141 0.0015 0.0037 0.0025 0.0184 0.003 0.0038 0.0018 0.0012 0.003 0 0.0004
Lipid Particles 0.0141 0 0.0039 0 0.0127 0.0017 0.0278 0.0018 0.0082 0.01 0.0291 0.0044 0.0109 0.0003 0.0025 0.0008 0 0.0008
Mitochondria 0.0078 0 0.0057 0 0.003 0.0021 0.0398 0.0005 0.007 0.0006 0.0908 0.0021 0.0134 0.0004 0.0087 0.047 0.0001 0.0076
None 0.0039 0 0.0009 0 0.0003 0.0007 0.006 0.0002 0.0008 0.0016 0.0015 0.0001 0.0013 0 0.0073 0.0001 0 0.0001
Nuclear Periphery 0.0269 0.0009 0.0091 0.0003 0.0009 0.0144 0.0268 0.0021 0.0027 0.0049 0.0199 0.0044 0.0404 0.0015 0.007 0.0001 0.0004 0.001
Nucleolus 0.0118 0.0039 0.0051 0.0024 0.0296 0.0161 0.0113 0.0055 0.0075 0.0049 0.0119 0.011 0.0259 0.0038 0.0036 0.0014 0.0258 0.0129
Nucleus 0.8067 0.9947 0.946 0.9972 0.8414 0.9512 0.7343 0.9759 0.9 0.9272 0.7416 0.9631 0.8236 0.9616 0.9249 0.9216 0.9731 0.9526
Peroxisomes 0.0076 0 0.0007 0 0.0069 0.0002 0.0101 0.0005 0.0082 0.0015 0.004 0.0002 0.0098 0.0051 0.0027 0.0101 0 0.0005
Punctate Nuclear 0.0136 0.0003 0.0018 0 0.002 0.0014 0.0062 0.0035 0.0033 0.0153 0.0028 0.001 0.0161 0.0009 0.0035 0.0001 0.0002 0.0112
Vacuole 0.0044 0 0.0011 0 0.0011 0.0003 0.0057 0.0002 0.0036 0.0002 0.0056 0.0005 0.006 0.0063 0.0008 0.0001 0 0.0053
Vacuole Periphery 0.0027 0 0.0009 0 0.0003 0.0006 0.0041 0.0001 0.0017 0.0002 0.0043 0.0005 0.0036 0.0008 0.0004 0.0001 0 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 70.1587 87.4245 63.4625 58.9248 79.9379 80.744 58.1292 75.2241 72.4397 69.1743
Translational Efficiency 1.7682 1.6159 1.8831 2.5707 1.5853 1.6079 2.3315 2.0777 1.8194 1.8027

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ubiquitin-conjugating enzyme (E2); involved in postreplication repair as a heterodimer with Rad18p, regulation of K63 polyubiquitination in response to oxidative stress, DSBR and checkpoint control as a heterodimer with Bre1p, ubiquitin-mediated N-end rule protein degradation as a heterodimer with Ubr1p, ERAD with Ubr1p in the absence of canonical ER membrane ligases, and Rpn4p turnover as part of proteasome homeostasis, in complex with Ubr2p and Mub1p
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Rad6

Rad6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rad6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available