Standard name
Human Ortholog
Description Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0.06 0.07 0.09 0.05 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 0.99 0.98 0.94 0.91 0.83 0.86 0.84 0.83 0.83 0.99 0.98 0.98 0.92 0.91 0.97 0.98 0.97 0.93 0.95 0.94 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0.08 0.15 0.09 0.12 0.08 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 3 2 2 1 5 0 0 0 0 1 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1 2 1 0 0 1 2 0 0 0 0 0 1 0 0 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 7 2 0 0 7 9 19 33 17 33 3 15 1 0 3 3 5 2 0 0 0 0 0 0
Cytoplasm 372 94 188 196 314 263 388 429 215 312 50 114 219 186 196 228 127 176 364 92 182 125 162 251
Endoplasmic Reticulum 1 1 1 1 3 9 32 78 23 44 5 8 3 2 3 7 3 8 2 0 4 0 2 11
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 1 0 0 0 1 0 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Mitochondria 0 0 1 0 0 1 0 0 2 4 4 8 0 0 1 1 0 1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 1 0 1 0 1 0 0 0 14 7 3 0 0 3 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 3 0 1 1 2 1 1 0 2 0 6 2 2 0 0 2 0 2 2
Unique Cell Count 374 95 190 197 319 280 425 519 251 370 60 138 221 190 201 249 140 182 372 95 197 132 173 277
Labelled Cell Count 380 97 190 197 324 286 440 547 261 399 64 155 225 191 203 260 147 193 372 95 197 132 173 277


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 109.1 96.1 76.7 69.6 76.3 68.7 64.3 59.0 52.2 52.8 46.2 48.4 99.5 91.9 77.1 113.4 130.8 117.8 125.6 115.3 121.1
Std Deviation (1e-4) 26.1 24.0 21.9 19.3 20.0 18.8 17.5 17.0 16.9 16.2 19.9 22.8 28.5 27.5 25.9 58.9 63.4 54.9 38.5 38.1 40.8
Intensity Change (Log2) – – – -0.14 -0.01 -0.16 -0.25 -0.38 -0.55 -0.54 -0.73 -0.66 0.38 0.26 0.01 0.56 0.77 0.62 0.71 0.59 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 3.0 3.6 3.7 4.2 0 4.7 0 0 0 0 0 0
Cytoplasm 0.6 -0.5 -2.7 -3.6 -5.7 -4.9 -5.3 -4.9 -5.4 0.2 -0.8 -1.1 -3.4 -3.5 -1.5
Endoplasmic Reticulum 0 0 0 3.6 5.4 4.0 4.7 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 3.3 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 68.7857 93.7456 83.2584 76.6538 73.3574 81.2876 181.5346 262.4547 236.599 193.4113 192.1365 241.7126 139.3227 176.31 163.0641 136.9116 135.0935 130.9777
Actin 0.001 0.0008 0.0038 0.0004 0.0013 0.002 0.025 0.0018 0.0003 0.0009 0.004 0.0002 0.0064 0.0002 0.0047 0.0362 0.0002 0.0004
Bud 0.0015 0.0025 0.0019 0.0034 0.003 0.0021 0.0016 0.0009 0.0007 0.0018 0.0008 0.0004 0.001 0.0004 0.0009 0.0029 0.001 0.0013
Bud Neck 0.0011 0.0012 0.0012 0.0022 0.003 0.0118 0.001 0.0008 0.0008 0.001 0.0016 0.0018 0.0006 0.0005 0.0008 0.0017 0.0007 0.0007
Bud Periphery 0.0021 0.0017 0.002 0.0035 0.005 0.0031 0.0021 0.001 0.0003 0.0007 0.0005 0.0004 0.001 0.0001 0.0003 0.0025 0.0001 0.0008
Bud Site 0.0008 0.0023 0.0035 0.0008 0.0003 0.0006 0.001 0.0015 0.0004 0.0007 0.0002 0.0001 0.0008 0.0004 0.0006 0.0062 0.0001 0.0003
Cell Periphery 0.0169 0.0185 0.0194 0.012 0.0256 0.0057 0.0241 0.0304 0.013 0.0158 0.0076 0.0271 0.0143 0.0089 0.0126 0.0104 0.0006 0.0051
Cytoplasm 0.6177 0.8237 0.7912 0.6122 0.6689 0.7192 0.7291 0.9075 0.9134 0.6651 0.8103 0.8631 0.8052 0.9593 0.9093 0.7296 0.9204 0.8061
Cytoplasmic Foci 0.0011 0.0012 0.0013 0.0013 0.0021 0.0052 0.0077 0.0003 0.0007 0.0024 0.0018 0.0012 0.0051 0.0006 0.0062 0.0039 0.004 0.0022
Eisosomes 0.0003 0 0 0 0.0001 0.0001 0.0017 0.0002 0.0001 0.004 0 0 0.0001 0 0 0.0007 0 0
Endoplasmic Reticulum 0.05 0.0185 0.0164 0.0106 0.0484 0.067 0.0194 0.0012 0.0041 0.0009 0.002 0.0025 0.0159 0.0024 0.007 0.004 0.0006 0.0063
Endosome 0.0059 0.0022 0.0035 0.0067 0.0156 0.0172 0.002 0.0003 0.0005 0.0023 0.0295 0.0014 0.0065 0.0006 0.0022 0.0054 0.0058 0.0022
Golgi 0.0007 0.0006 0.0004 0.0002 0.002 0.0076 0.0012 0.0001 0.0001 0.0003 0.0475 0.0001 0.0027 0.0001 0.0012 0.0022 0.0003 0.0001
Lipid Particles 0.0005 0.0015 0.0011 0.0004 0.0014 0.0003 0.0063 0.0013 0.0005 0.0027 0.0005 0.0006 0.0027 0.0005 0.0009 0.0028 0.0002 0.0007
Mitochondria 0.0053 0.0046 0.001 0.0009 0.0426 0.0886 0.0074 0.0038 0.0006 0.0114 0.0292 0.0004 0.0109 0.0001 0.004 0.0067 0.0016 0.005
None 0.0008 0.0005 0.0005 0.0003 0.0005 0.0005 0.0062 0.0006 0.0007 0.0074 0.0003 0.0004 0.0043 0.0002 0.0004 0.0016 0.0011 0.0009
Nuclear Periphery 0.0547 0.0082 0.0094 0.0105 0.0186 0.0053 0.0665 0.0069 0.0083 0.0115 0.0077 0.0082 0.0301 0.0022 0.0043 0.0104 0.0031 0.0172
Nucleolus 0.0004 0.0008 0.0005 0.0019 0.0003 0.0001 0.0061 0.0015 0.0027 0.0508 0.0011 0.0029 0.0101 0.0004 0.0015 0.002 0.0006 0.0679
Nucleus 0.0127 0.0047 0.0052 0.0086 0.0058 0.0034 0.0306 0.006 0.0067 0.0255 0.0084 0.0091 0.0128 0.0019 0.0034 0.01 0.0052 0.0196
Peroxisomes 0.0001 0.0001 0.0001 0.0001 0.0002 0.0002 0.0043 0.0001 0.0001 0.0003 0.0001 0 0.0007 0 0.0007 0.0031 0.0001 0.0002
Punctate Nuclear 0.0001 0 0.0001 0 0.0001 0.0001 0.0017 0 0.0001 0.0009 0.0001 0.0001 0.0008 0 0.0001 0.0008 0.0001 0.0001
Vacuole 0.1728 0.0926 0.1146 0.2665 0.1066 0.0459 0.0404 0.0312 0.0378 0.12 0.0328 0.0687 0.0535 0.0192 0.0337 0.1227 0.0344 0.039
Vacuole Periphery 0.0535 0.0138 0.0228 0.0576 0.0488 0.0141 0.015 0.0025 0.0081 0.0737 0.0142 0.0114 0.0144 0.0018 0.0052 0.0343 0.0198 0.0238

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 234.1264 119.6368 97.8809 126.023 83.3209 284.9741 109.8269 118.9437 163.3651 101.1211
Translational Efficiency 0.6566 0.5716 0.5002 0.6213 0.5377 0.578 0.5751 0.463 0.4431 0.4477

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
457 34 245 1623 1342 1328 397 1412 1799 1362 642 3035

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1720.24 313.43 2035.07 5278.91 2673.60 3246.30 2475.63 5796.72 2431.42 3173.09 2307.50 5519.82
Standard Deviation 663.31 21.24 489.94 1272.16 751.83 978.06 594.40 1504.73 840.04 1068.69 596.57 1409.10
Intensity Change Log 2 — -2.456395 0.242469 1.617630 — 0.280013 -0.110988 1.116453 — -0.303715 0.037869 1.333835

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000338 0.000047 0.000857 0.000150 0.000081 0.000145 0.000216 0.000134 0.000146 0.000143 0.000461 0.000142
Bud Neck 0.000655 0.000016 0.008700 0.001255 0.000486 0.000733 0.003499 0.001692 0.000529 0.000715 0.005484 0.001458
Bud Site 0.013480 0.000032 0.018281 0.004205 0.005243 0.005580 0.009280 0.005331 0.007336 0.005441 0.012715 0.004729
Cell Periphery 0.001675 0.000074 0.001610 0.006200 0.003625 0.003649 0.001154 0.008628 0.003130 0.003560 0.001328 0.007329
Cytoplasm 0.860363* 0.000050 0.614116* 0.856189* 0.875707* 0.895131* 0.827239* 0.803521* 0.871809* 0.872787* 0.745907* 0.831686*
Cytoplasmic Foci 0.024098 0.000020 0.056121 0.004202 0.012295 0.015073 0.018626 0.005573 0.015294 0.014697 0.032935 0.004840
Eisosomes 0.000003 0.000083 0.000009 0.000002 0.000001 0.000001 0.000003 0.000003 0.000001 0.000003 0.000005 0.000002
Endoplasmic Reticulum 0.001012 0.000001 0.000911 0.000073 0.000749 0.000334 0.000186 0.000069 0.000816 0.000326 0.000463 0.000071
Endosome 0.002321 0.000002 0.010266 0.000239 0.001164 0.000839 0.002075 0.001308 0.001458 0.000818 0.005201 0.000736
Golgi 0.024888 0.000006 0.008027 0.004509 0.025042 0.019481 0.003563 0.005168 0.025003 0.018994 0.005266 0.004816
Lipid Particles 0.002105 0.000034 0.012613 0.007615 0.005041 0.004340 0.006953 0.009725 0.004295 0.004233 0.009113 0.008597
Mitochondria 0.000732 0.000011 0.000082 0.000190 0.000184 0.000863 0.000051 0.000367 0.000324 0.000842 0.000063 0.000272
Mitotic Spindle 0.004030 0.000359 0.013931 0.000624 0.001413 0.002290 0.005569 0.001011 0.002078 0.002242 0.008760 0.000804
None 0.004455 0.999046* 0.001186 0.002255 0.001573 0.002443 0.001397 0.002615 0.002305 0.027321 0.001316 0.002423
Nuclear Periphery 0.000136 0.000000 0.000618 0.000183 0.000326 0.000462 0.000206 0.000292 0.000278 0.000451 0.000363 0.000234
Nuclear Periphery Foci 0.004916 0.000049 0.010474 0.002892 0.005447 0.004530 0.004998 0.002812 0.005312 0.004419 0.007088 0.002855
Nucleolus 0.000448 0.000045 0.000161 0.000119 0.000068 0.000153 0.000154 0.000232 0.000164 0.000150 0.000157 0.000172
Nucleus 0.015377 0.000014 0.031565 0.035930 0.006542 0.016577 0.038042 0.030788 0.008786 0.016164 0.035570 0.033538
Peroxisomes 0.000181 0.000109 0.000285 0.000041 0.000046 0.000051 0.000132 0.000087 0.000080 0.000053 0.000191 0.000062
Vacuole 0.016524 0.000002 0.205839* 0.070384 0.030443 0.017485 0.075537 0.114813 0.026907 0.017049 0.125263 0.091054
Vacuole Periphery 0.022262 0.000000 0.004348 0.002743 0.024523 0.009837 0.001120 0.005832 0.023949 0.009591 0.002352 0.004180

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 4.62 -4.72 1.96 -4.03 6.26 -2.54 -8.18 -3.76 -0.08 4.59 -0.20 -7.84 -1.23 -0.79 7.04
Bud Neck 5.76 -12.05 -5.74 -9.67 10.44 -2.50 -9.23 -9.38 -7.13 5.40 -2.15 -14.72 -10.96 -7.70 11.04
Bud Site 8.34 -3.38 4.66 -7.16 8.79 -0.58 -4.63 -1.18 -0.67 3.69 2.43 -6.79 2.24 -0.22 8.40
Cell Periphery 5.76 0.51 -8.46 -13.52 -9.25 0.02 6.36 -5.25 -5.25 -9.66 -0.87 6.08 -7.57 -6.08 -13.59
Cytoplasm 87.41* 14.65 3.31 -153.60* -14.00 -2.20 9.75 12.59 15.26 -1.56 0.18 16.65 11.56 9.88 -10.67
Cytoplasmic Foci 10.48 -7.33 7.81 -11.39 12.62 -2.32 -5.43 5.87 7.30 9.08 0.06 -9.59 9.90 7.95 14.74
Eisosomes -2.91 -7.05 -0.34 2.90 9.64 -0.97 -8.62 -6.26 -5.19 2.65 -2.25 -11.64 -4.80 0.26 8.76
Endoplasmic Reticulum 5.66 0.61 5.23 -7.28 6.03 2.75 4.38 5.53 3.17 4.60 3.69 3.09 7.27 3.12 6.99
Endosome 6.55 -8.65 5.79 -5.99 11.45 1.73 -4.04 0.25 -0.63 3.03 3.41 -9.39 3.86 0.94 11.02
Golgi 9.80 6.58 7.85 -5.94 1.91 2.35 11.99 11.09 9.34 -3.02 2.83 13.16 13.35 9.38 0.12
Lipid Particles 5.59 -7.68 -9.25 -14.03 3.52 0.69 -3.18 -4.89 -6.46 -1.00 -0.06 -6.66 -6.94 -7.59 1.79
Mitochondria 1.37 1.19 0.93 -3.72 -2.06 -1.01 3.67 -1.21 0.78 -2.31 -0.77 1.79 0.30 0.85 -3.16
Mitotic Spindle 2.88 -4.23 2.63 -1.65 6.14 -1.54 -3.50 0.99 2.12 3.74 -0.31 -5.81 2.96 2.84 6.89
None -659.32* 2.32 1.52 3703.08* -3.94 -1.99 1.18 -1.20 0.20 -1.69 -5.89 2.73 0.17 5.92 -3.25
Nuclear Periphery 3.45 -7.56 -1.66 -10.47 7.32 -0.45 -0.23 0.12 0.75 0.93 -0.67 -1.63 0.14 0.96 5.44
Nuclear Periphery Foci 6.70 -6.23 2.14 -12.17 9.26 1.17 -0.65 4.46 2.89 4.80 1.24 -4.41 4.74 2.51 9.48
Nucleolus 1.48 0.90 1.15 -4.97 2.90 -1.77 -5.77 -3.98 -1.24 -1.14 -0.02 -0.52 -0.37 -0.45 0.38
Nucleus 5.20 -4.64 -7.93 -24.66 -1.14 -7.44 -11.48 -15.57 -9.18 1.89 -5.21 -12.66 -20.43 -13.08 0.87
Peroxisomes 0.65 -1.29 1.31 2.06 9.58 -0.48 -2.11 -1.19 -0.80 1.54 0.80 -3.12 0.74 -0.11 4.05
Vacuole 5.73 -13.38 -14.73 -22.56 9.00 4.45 -10.16 -18.92 -22.13 -0.65 3.86 -16.03 -22.61 -26.43 6.47
Vacuole Periphery 5.77 4.79 5.08 -9.87 0.88 6.09 11.27 8.82 3.28 -5.97 6.57 11.82 10.78 4.61 -3.96
Standard name
Human Ortholog
Description Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Pyc1

Pyc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pyc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available