Standard name
Human Ortholog
Description RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.07 0 – 0 0 0 0 0 0 0.1 0.13 0.13 0.13 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 – 0 0.06 0 0.07 0.1 0 0 0 0 0.1 0 0 0 0 0 0 0
Nucleus 0.81 0.69 0.74 0.76 0.7 0.68 – 0.68 0.68 0.71 0.66 0.64 0.7 0.68 0.63 0.65 0.6 0.64 0.78 0.65 0.63 0.54 0.53 0.52
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.5 0.69 0.61 0.47 0.43 0.55 – 0.5 0.64 0.51 0.62 0.57 0.6 0.47 0.35 0.32 0.34 0.35 0.17 0.3 0.29 0.35 0.34 0.33
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 1 0 0 0 0 1 – 1 0 5 1 2 1 0 0 0 0 0 3 1 4 1 5 3
Bud Neck 2 0 1 0 1 1 – 3 1 5 1 3 0 0 1 0 0 0 1 0 0 0 0 0
Bud Site 0 0 13 2 1 3 – 9 13 13 17 26 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 4 11 14 18 13 – 9 11 15 12 11 6 19 26 15 12 9 0 2 1 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 1 1 3 0 0 0 0 1 0
Endosome 4 0 0 0 0 0 – 1 0 0 0 0 2 1 4 2 2 2 5 0 2 3 3 5
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 1 0 1 0 0 0 0 0 0 3 3 5
Mitochondria 7 0 15 11 2 15 – 15 23 15 29 42 1 0 2 3 9 4 3 0 3 0 0 0
Nucleus 510 99 281 229 186 204 – 279 265 237 269 265 138 133 122 74 54 84 519 106 252 117 138 147
Nuclear Periphery 0 1 1 1 2 2 – 2 5 4 0 5 0 0 0 1 1 1 0 0 0 0 1 1
Nucleolus 311 100 233 142 114 165 – 206 250 170 254 238 118 92 67 36 31 46 111 49 118 76 88 94
Peroxisomes 2 0 1 1 0 0 – 2 0 1 0 0 1 3 3 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 1 – 2 0 2 0 0 2 4 6 2 0 0 3 0 2 2 2 3
Vac/Vac Membrane 1 0 0 2 0 0 – 2 0 2 1 1 3 3 3 1 3 6 13 1 12 9 12 18
Unique Cell Count 627 144 380 301 265 301 409 390 336 410 415 197 196 193 113 90 131 665 163 403 218 262 283
Labelled Cell Count 842 204 556 402 324 405 531 568 469 584 593 274 255 236 135 113 155 665 163 403 218 262 283


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.3 20.8 11.0 14.9 13.2 10.8 – 11.0 9.8 10.3 9.4 9.3 18.2 17.9 15.8 19.1 17.8 18.5 16.8 17.1 18.6
Std Deviation (1e-4) 4.4 7.3 4.3 8.2 6.3 4.5 – 4.3 4.1 4.5 4.4 3.5 6.5 8.0 7.2 10.1 7.3 8.7 5.1 5.9 9.2
Intensity Change (Log2) – – – 0.44 0.27 -0.02 – 0.0 -0.16 -0.09 -0.22 -0.24 0.73 0.71 0.53 0.8 0.7 0.75 0.62 0.64 0.76


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site -2.4 -2.6 -2.1 – -1.0 -0.1 0.3 0.5 1.9 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.2 2.4 1.0 – -0.6 -0.1 1.1 0 -0.2 0.1 3.5 4.9 4.3 0 2.0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.2 -2.5 0.7 – -0.2 1.2 0.3 1.9 3.4 -2.4 -2.8 -1.9 0 0 0
Nucleus 0.6 -1.1 -1.8 – -1.8 -1.8 -1.0 -2.5 -3.1 -1.0 -1.5 -2.7 -1.8 -2.6 -2.1
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -3.7 -4.6 -1.7 – -3.1 0.8 -2.9 0.2 -1.1 -0.3 -3.3 -6.0 -5.5 -4.6 -5.2
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.8931 -0.4118 -0.629 -0.7769 -0.9796 -0.6513 -1.5845 -1.2148 -1.3854 -1.432 -0.7107 -1.1054 -0.3562 -0.0404 -0.0267 -0.0849 0.7084 -0.0259
Actin 0.0008 0.0001 0.0006 0.0002 0 0.0001 0.009 0.0046 0.0072 0.0001 0.0521 0.0026 0 0.0001 0.0001 0 0 0
Bud 0.0002 0.0018 0.0012 0.0001 0 0.0026 0.0015 0.0043 0.0007 0 0.0018 0.0006 0 0 0.0048 0 0 0
Bud Neck 0.0002 0.0015 0.0003 0.0004 0.0001 0.0002 0.0027 0.0004 0.0003 0 0.0134 0.0022 0 0.0001 0.0008 0 0 0.0001
Bud Periphery 0.0002 0.0011 0.0013 0.0001 0 0.0026 0.0051 0.0043 0.0007 0.0001 0.0039 0.0012 0 0 0.0048 0 0 0
Bud Site 0.0034 0.017 0.0118 0.0005 0 0.0003 0.0055 0.0117 0.0067 0.0001 0.0107 0.0005 0 0.0004 0.004 0 0 0
Cell Periphery 0.0001 0.0002 0.0002 0 0 0.0001 0.0005 0.0005 0.0001 0 0.0011 0.0001 0 0.0001 0.0001 0 0 0
Cytoplasm 0.0171 0.0231 0.0136 0.0158 0.0078 0.0203 0.0238 0.028 0.0137 0.0124 0.0108 0.0264 0.0066 0.0259 0.0112 0.0065 0.01 0.0175
Cytoplasmic Foci 0.0055 0.0094 0.0039 0.0036 0.0068 0.0066 0.0124 0.0064 0.0137 0.0038 0.0104 0.0057 0.0006 0.0016 0.0089 0.0009 0.0012 0.0017
Eisosomes 0.0002 0.0001 0.0002 0.0003 0.0001 0.0001 0.0004 0.0003 0.0003 0.0001 0.0011 0.0001 0.0002 0.0002 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0003 0.0003 0.0002 0.0002 0.0001 0.0001 0.0017 0.0005 0.0003 0.0002 0.002 0.0007 0.0002 0.0003 0.0002 0.0002 0.0002 0.0001
Endosome 0.0013 0.0016 0.0002 0.0005 0.0001 0.0003 0.0039 0.0003 0.0003 0.0002 0.0114 0.0021 0.0001 0.0001 0.0013 0.0001 0.0001 0.0001
Golgi 0.0004 0.0002 0.0001 0 0 0 0.0011 0.0001 0.0001 0 0.0048 0.001 0 0 0.0002 0 0 0
Lipid Particles 0.0013 0.0005 0.0002 0.0001 0.0002 0.0001 0.0037 0.0004 0.0005 0.0002 0.0081 0.0028 0.0001 0.0002 0.0003 0.0001 0.0003 0.0001
Mitochondria 0.0005 0.0002 0.0002 0.0001 0.0001 0.0001 0.001 0.0009 0.0005 0.0002 0.0024 0.0021 0 0.0001 0.0003 0.0001 0.0001 0
None 0.9657 0.9336 0.9561 0.9739 0.978 0.9624 0.9144 0.9219 0.9483 0.9797 0.8002 0.9343 0.9921 0.97 0.9593 0.9915 0.9878 0.9756
Nuclear Periphery 0.0003 0.0002 0.0004 0.0002 0.0002 0.0002 0.0022 0.0011 0.0003 0.0001 0.0099 0.0016 0 0.0001 0.0001 0.0001 0 0.0001
Nucleolus 0.0003 0.0005 0.0008 0.0006 0.0004 0.0002 0.0008 0.0012 0.0005 0.0004 0.0066 0.0007 0 0.0001 0.0009 0 0 0.0003
Nucleus 0.0005 0.0012 0.0022 0.0007 0.0008 0.0013 0.0015 0.0054 0.0009 0.0004 0.0063 0.0025 0 0.0002 0.0005 0.0001 0 0.0008
Peroxisomes 0.0007 0.0003 0.0002 0.0001 0.0002 0.001 0.0047 0.0008 0.002 0.0001 0.0047 0.0077 0 0.0001 0.0006 0 0 0
Punctate Nuclear 0.001 0.0067 0.0059 0.0024 0.0052 0.0011 0.003 0.0064 0.0026 0.0014 0.0319 0.0042 0.0001 0.0003 0.0005 0.0001 0 0.0033
Vacuole 0.0002 0.0002 0.0003 0.0002 0.0001 0.0002 0.0009 0.0005 0.0002 0.0002 0.0054 0.0005 0.0001 0.0001 0.001 0.0001 0.0001 0.0001
Vacuole Periphery 0 0 0 0 0 0 0.0002 0.0001 0 0 0.001 0.0002 0 0 0 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 32.8928 42.7697 33.7205 38.8197 42.647 32.8746 46.6229 36.4838 37.6124 41.016
Translational Efficiency 3.7075 2.9467 3.2037 2.5188 2.5497 2.996 2.2344 2.504 2.1408 2.5486

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1426 172 1772 712 1510 2015 205 1478 2936 2187 1977 2190

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 598.98 683.03 733.12 738.17 583.42 685.67 733.46 771.35 590.98 685.46 733.16 760.56
Standard Deviation 65.41 92.12 104.78 96.46 71.25 84.82 87.10 116.65 68.91 85.42 103.09 111.58
Intensity Change Log 2 — 0.189441 0.291542 0.301445 — 0.232980 0.330183 0.402851 — 0.211088 0.310738 0.352372

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000087 0.000289 0.000112 0.000397 0.000078 0.000458 0.000491 0.001129 0.000082 0.000445 0.000152 0.000891
Bud Neck 0.023346 0.056698 0.002555 0.006595 0.016304 0.072309 0.001593 0.010295 0.019724 0.071081 0.002455 0.009092
Bud Site 0.002994 0.014682 0.007196 0.010209 0.002335 0.017980 0.004204 0.033150 0.002655 0.017720 0.006885 0.025691
Cell Periphery 0.000130 0.000246 0.000123 0.000203 0.000148 0.000275 0.000212 0.000176 0.000139 0.000273 0.000132 0.000185
Cytoplasm 0.429400* 0.192173* 0.345556* 0.459856* 0.418648* 0.296692* 0.294026* 0.465392* 0.423870* 0.288472* 0.340213* 0.463592*
Cytoplasmic Foci 0.301534* 0.387702* 0.011900 0.034107 0.262607* 0.298319* 0.021766 0.025656 0.281514* 0.305349* 0.012923 0.028403
Eisosomes 0.000236 0.000302 0.000028 0.000066 0.000244 0.000247 0.000090 0.000049 0.000240 0.000252 0.000035 0.000055
Endoplasmic Reticulum 0.000573 0.000718 0.001113 0.002451 0.000533 0.000667 0.003250 0.001293 0.000552 0.000671 0.001335 0.001670
Endosome 0.013177 0.055665 0.001347 0.004045 0.009645 0.040360 0.002485 0.009110 0.011361 0.041563 0.001465 0.007463
Golgi 0.002971 0.007915 0.000105 0.004802 0.001671 0.011693 0.000074 0.008240 0.002302 0.011396 0.000102 0.007123
Lipid Particles 0.014561 0.021529 0.000922 0.001988 0.020678 0.016068 0.003021 0.001337 0.017707 0.016498 0.001140 0.001549
Mitochondria 0.005533 0.023315 0.001053 0.000915 0.007805 0.039506 0.000336 0.007741 0.006701 0.038233 0.000978 0.005522
Mitotic Spindle 0.000104 0.000983 0.001974 0.015207 0.000374 0.010058 0.000217 0.057313 0.000243 0.009344 0.001792 0.043624
None 0.002916 0.001664 0.007341 0.006129 0.002825 0.003043 0.006436 0.006150 0.002869 0.002935 0.007247 0.006143
Nuclear Periphery 0.000437 0.000396 0.001143 0.001367 0.000947 0.000546 0.001271 0.001381 0.000699 0.000535 0.001157 0.001376
Nuclear Periphery Foci 0.000204 0.000318 0.000256 0.001594 0.000242 0.000268 0.001078 0.000605 0.000224 0.000272 0.000341 0.000926
Nucleolus 0.001312 0.002039 0.000505 0.000438 0.001807 0.002008 0.000925 0.000636 0.001567 0.002010 0.000549 0.000572
Nucleus 0.150246* 0.095187 0.595289* 0.322029* 0.200817* 0.080046 0.598472* 0.282427* 0.176255* 0.081237 0.595619* 0.295302*
Peroxisomes 0.008087 0.047142 0.000290 0.000835 0.010247 0.028469 0.000554 0.000858 0.009198 0.029938 0.000317 0.000850
Vacuole 0.040998 0.087303 0.020966 0.126356 0.041227 0.076583 0.059292 0.084643 0.041116 0.077426 0.024940 0.098204
Vacuole Periphery 0.001157 0.003736 0.000224 0.000412 0.000816 0.004403 0.000205 0.002418 0.000981 0.004351 0.000222 0.001766

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.34 -1.25 -4.36 -0.89 -3.62 -4.95 -1.77 -2.85 -0.86 0.03 -5.13 -2.29 -3.49 -0.71 -2.67
Bud Neck -3.65 11.93 8.47 5.67 -3.09 -17.51 11.46 5.03 20.53 -6.65 -17.14 15.71 9.40 21.75 -6.32
Bud Site -3.19 -2.68 -4.22 1.17 -1.63 -12.35 -0.89 -8.02 -1.08 -6.24 -13.07 -3.06 -8.91 0.28 -5.59
Cell Periphery -2.53 1.02 -2.38 1.32 -2.70 -4.19 -1.91 1.37 4.83 2.32 -4.89 0.70 -0.77 4.48 -1.25
Cytoplasm 15.29 11.01 5.32 -9.81 -3.23 13.46 8.58 3.34 -9.12 -6.59 18.28 13.38 5.95 -10.69 -6.89
Cytoplasmic Foci -4.32 47.58 42.65 19.91 -6.58 -4.52 38.74 42.67 52.93 0.83 -3.46 63.82 61.07 55.75 -6.59
Eisosomes -2.37 22.60 15.62 7.98 -11.94 -0.19 7.64 15.15 18.06 3.21 -0.89 25.55 22.29 18.69 -9.89
Endoplasmic Reticulum -0.70 -9.01 -13.34 -8.00 -8.94 -1.13 -7.39 -11.13 -11.47 4.59 -1.34 -11.72 -17.56 -15.71 -5.17
Endosome -5.83 13.88 10.93 7.28 -4.32 -17.73 9.58 4.96 20.28 -2.86 -18.09 17.72 9.77 22.49 -5.50
Golgi -2.47 8.24 0.67 2.48 -1.68 -9.99 6.99 -1.63 5.86 -3.25 -9.70 10.66 -0.81 6.92 -3.64
Lipid Particles -1.52 14.64 13.27 4.61 -5.88 2.33 9.94 11.22 14.29 4.20 0.95 16.56 16.19 14.82 -3.24
Mitochondria -2.97 5.47 6.18 3.86 0.75 -12.10 5.95 2.56 14.47 -3.99 -13.73 7.26 4.03 15.73 -2.92
Mitotic Spindle -3.59 -3.20 -4.60 -4.28 -3.75 -6.90 1.35 -9.00 -5.87 -9.05 -7.05 -2.97 -10.04 -5.85 -9.12
None 1.23 -5.05 -1.17 -1.87 2.07 -0.35 -2.76 -2.76 -1.91 0.59 -0.17 -6.02 -2.85 -2.03 2.70
Nuclear Periphery 0.18 -13.95 -11.05 -10.83 -4.73 1.89 -3.13 -7.21 -14.41 -6.43 1.20 -6.89 -13.18 -15.43 -9.95
Nuclear Periphery Foci -1.89 -3.80 -6.93 -5.98 -6.36 -0.46 -6.32 -7.38 -6.61 3.91 -1.28 -6.06 -9.67 -8.65 -6.71
Nucleolus -1.65 4.65 3.33 4.34 -0.41 -0.52 3.51 4.54 7.99 1.86 -1.57 6.30 5.89 8.29 -0.24
Nucleus 4.46 -54.99 -17.25 -16.14 22.40 21.21 -20.19 -15.87 -33.46 12.62 22.36 -57.14 -24.20 -39.83 30.05
Peroxisomes -5.75 10.16 9.68 6.96 -2.25 -10.25 9.29 9.48 20.06 0.50 -13.30 13.65 13.36 21.14 -2.14
Vacuole -5.01 4.77 -19.08 -9.24 -20.26 -10.63 -4.83 -21.10 -14.56 -6.84 -12.56 2.39 -28.37 -19.36 -28.37
Vacuole Periphery -2.58 4.77 4.42 3.60 -0.64 -7.71 6.19 -1.12 6.38 -3.77 -7.65 6.87 0.78 7.68 -3.68
Standard name
Human Ortholog
Description RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Rpb9

Rpb9


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpb9-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available