Standard name | |
---|---|
Human Ortholog | |
Description | Trimeric heat shock transcription factor; activates multiple genes in response to highly diverse stresses; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; monitors translational status of cell through an RQC (Ribosomal Quality Control)-mediated translation-stress signal; involved in diauxic shift; posttranslationally regulated; human homolog HSF1 with linker region mutations can complement yeast hsf1 mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud | 0 | 1 | 0 | 0 | 2 | 1 | 6 | 1 | 10 | – | 2 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 2 | 5 | 0 |
Bud Neck | 0 | 0 | 2 | 2 | 1 | 1 | 1 | 3 | 0 | – | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 |
Bud Site | 0 | 0 | 2 | 4 | 3 | 6 | 18 | 6 | 19 | – | 8 | 12 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 7 | 9 | 16 | 16 | 23 | 22 | 23 | 13 | – | 10 | 10 | 5 | 34 | 29 | 10 | 5 | 5 | 0 | 3 | 5 | 0 | 0 | 1 |
Endoplasmic Reticulum | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 5 | 1 | 1 | 0 | 0 | 1 | 2 | 0 | 1 |
Endosome | 0 | 1 | 2 | 1 | 1 | 1 | 1 | 2 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 3 | 4 | 10 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 16 | 22 | 30 | 19 | 20 | 78 | 119 | 88 | 155 | – | 61 | 85 | 26 | 8 | 12 | 3 | 3 | 4 | 0 | 2 | 4 | 1 | 2 | 4 |
Nucleus | 166 | 211 | 318 | 158 | 209 | 247 | 328 | 289 | 207 | – | 86 | 180 | 109 | 301 | 263 | 52 | 59 | 47 | 164 | 219 | 296 | 88 | 164 | 121 |
Nuclear Periphery | 0 | 3 | 2 | 1 | 2 | 0 | 3 | 7 | 6 | – | 1 | 0 | 0 | 1 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 4 | 3 | 4 | 3 | 4 | 6 | 16 | 9 | 18 | – | 12 | 13 | 13 | 6 | 8 | 1 | 7 | 3 | 0 | 3 | 14 | 22 | 36 | 39 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 13 | 5 | 4 | 8 | 4 | 16 | 3 | – | 6 | 6 | 1 | 1 | 2 | 0 | 1 | 1 | 1 | 1 | 1 | 4 | 1 | 9 |
Vac/Vac Membrane | 2 | 7 | 2 | 1 | 0 | 3 | 1 | 1 | 0 | – | 1 | 2 | 1 | 5 | 5 | 0 | 4 | 1 | 5 | 11 | 20 | 12 | 27 | 23 |
Unique Cell Count | 174 | 236 | 337 | 177 | 237 | 305 | 385 | 353 | 261 | 114 | 220 | 116 | 346 | 306 | 67 | 73 | 59 | 176 | 250 | 354 | 141 | 252 | 221 | |
Labelled Cell Count | 191 | 256 | 384 | 210 | 262 | 374 | 519 | 445 | 431 | 188 | 321 | 155 | 360 | 323 | 73 | 81 | 64 | 176 | 250 | 354 | 141 | 252 | 221 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 5.8 | 5.6 | 5.3 | 5.8 | 4.8 | 5.0 | 5.0 | 4.2 | – | 4.1 | 4.1 | 5.5 | 5.7 | 5.7 | 7.7 | 9.0 | 8.5 | 5.1 | 5.4 | 5.7 |
Std Deviation (1e-4) | 0.7 | 1.3 | 0.9 | 1.5 | 1.5 | 1.3 | 1.5 | 1.3 | 1.5 | – | 1.5 | 0.9 | 1.1 | 1.2 | 1.6 | 1.4 | 2.0 | 2.0 | 1.1 | 1.1 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.05 | -0.22 | -0.17 | -0.16 | -0.41 | – | -0.44 | -0.44 | -0.03 | 0.02 | 0.02 | 0.47 | 0.68 | 0.6 | -0.13 | -0.05 | 0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 3.3 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3.2 | 2.4 | 2.8 | 2.0 | 2.4 | 1.5 | – | 0 | 1.2 | 0 | 3.8 | 3.7 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.7 | -0.2 | 0 | 7.3 | 5.6 | 0 | – | 0 | 0 | 3.8 | -3.8 | -2.6 | -1.2 | -1.4 | -0.5 |
Nucleus | -2.1 | -2.7 | -5.2 | -4.0 | -5.0 | -5.6 | – | -5.7 | -4.7 | -0.2 | -3.3 | -3.6 | -4.5 | -3.9 | -3.9 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 2.4 | 1.3 | 0 | – | 0 | 0 | 0 | 0 | 1.3 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -0.6 | -1.5 | 0 | -2.5 | 0.4 | 0 | – | 0 | 0 | 0 | -3.3 | -2.7 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.1314 | 3.9814 | 3.3048 | 3.2047 | 2.3818 | 3.1349 | 2.1364 | 2.3468 | 1.9486 | 1.8091 | 2.0213 | 1.8786 | 1.4904 | 1.0863 | 1.3538 | 0.9621 | 2.0994 | 1.0055 |
Actin | 0.0293 | 0 | 0.0141 | 0 | 0.0589 | 0.0001 | 0.0094 | 0.0002 | 0.004 | 0.0039 | 0.0081 | 0.0052 | 0.0072 | 0 | 0.0108 | 0.0234 | 0.0322 | 0.0059 |
Bud | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0006 | 0 | 0.0004 | 0.0006 | 0.0019 | 0.0004 | 0.0001 | 0 | 0.0003 | 0.0006 | 0.0002 | 0.0002 |
Bud Neck | 0.0023 | 0 | 0.0005 | 0 | 0.0003 | 0.0008 | 0.0021 | 0.0001 | 0.0006 | 0.0004 | 0.0013 | 0.0011 | 0.0003 | 0.0001 | 0.0004 | 0.0004 | 0.0009 | 0.0008 |
Bud Periphery | 0.0006 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0014 | 0 | 0.0007 | 0.0011 | 0.0081 | 0.0009 | 0.0002 | 0 | 0.0002 | 0.0007 | 0.0003 | 0.0006 |
Bud Site | 0.0038 | 0 | 0.0009 | 0 | 0.0003 | 0 | 0.0026 | 0.0001 | 0.0033 | 0.002 | 0.0044 | 0.0013 | 0.0004 | 0 | 0.0031 | 0.0052 | 0.001 | 0.0004 |
Cell Periphery | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0004 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.0035 | 0.0001 | 0.0015 | 0 | 0.0002 | 0.0002 | 0.0086 | 0.0163 | 0.0028 | 0.0007 | 0.0004 | 0.0015 | 0.0161 | 0.0002 | 0.0028 | 0.0006 | 0.0005 | 0.0001 |
Cytoplasmic Foci | 0.0077 | 0 | 0.0069 | 0 | 0.001 | 0.0001 | 0.0157 | 0.0011 | 0.0111 | 0.0039 | 0.01 | 0.0008 | 0.0125 | 0 | 0.0122 | 0.0039 | 0.0071 | 0.0009 |
Eisosomes | 0.0012 | 0 | 0.0002 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0001 |
Endoplasmic Reticulum | 0.0026 | 0 | 0.0004 | 0 | 0.0002 | 0 | 0.0024 | 0.001 | 0.002 | 0.0005 | 0.0006 | 0.0007 | 0.0102 | 0 | 0.0005 | 0.0008 | 0.0006 | 0.0001 |
Endosome | 0.0162 | 0 | 0.0045 | 0 | 0.0004 | 0.0002 | 0.0238 | 0.0016 | 0.0174 | 0.0377 | 0.0171 | 0.0023 | 0.0179 | 0 | 0.0035 | 0.0118 | 0.007 | 0.0022 |
Golgi | 0.0075 | 0 | 0.0039 | 0 | 0.001 | 0.0001 | 0.0059 | 0.0001 | 0.011 | 0.0047 | 0.0099 | 0.0011 | 0.0039 | 0 | 0.0032 | 0.0074 | 0.0068 | 0.0035 |
Lipid Particles | 0.0128 | 0 | 0.0092 | 0 | 0.0006 | 0.002 | 0.0219 | 0.0003 | 0.0062 | 0.0051 | 0.0401 | 0.0011 | 0.0075 | 0 | 0.0014 | 0.0047 | 0.0335 | 0.0051 |
Mitochondria | 0.0063 | 0.0001 | 0.0073 | 0 | 0.0005 | 0.0074 | 0.0101 | 0.0002 | 0.0298 | 0.0038 | 0.0505 | 0.0092 | 0.0041 | 0.0001 | 0.0034 | 0.0043 | 0.0189 | 0.0168 |
None | 0.0049 | 0 | 0.0049 | 0 | 0.0008 | 0.0089 | 0.0133 | 0.0025 | 0.0003 | 0.0004 | 0.0003 | 0.0002 | 0.0104 | 0.0001 | 0.0003 | 0.0006 | 0.0018 | 0 |
Nuclear Periphery | 0.0037 | 0.0012 | 0.0028 | 0.0002 | 0.0054 | 0.0005 | 0.0236 | 0.0023 | 0.0046 | 0.0065 | 0.0065 | 0.0072 | 0.0093 | 0.0004 | 0.0012 | 0.0015 | 0.0017 | 0.0002 |
Nucleolus | 0.0124 | 0.0084 | 0.0075 | 0.0047 | 0.0296 | 0.0479 | 0.0205 | 0.0118 | 0.0101 | 0.0065 | 0.051 | 0.032 | 0.0112 | 0.012 | 0.0071 | 0.0129 | 0.0255 | 0.0328 |
Nucleus | 0.8648 | 0.9899 | 0.9187 | 0.9948 | 0.8933 | 0.9291 | 0.818 | 0.9601 | 0.882 | 0.9109 | 0.7366 | 0.9323 | 0.8731 | 0.9867 | 0.9437 | 0.916 | 0.8394 | 0.9277 |
Peroxisomes | 0.0089 | 0 | 0.0068 | 0 | 0.0023 | 0.0004 | 0.0044 | 0.0001 | 0.0075 | 0.0021 | 0.0093 | 0.0007 | 0.0029 | 0 | 0.0048 | 0.0029 | 0.0153 | 0.0011 |
Punctate Nuclear | 0.0057 | 0.0002 | 0.0091 | 0.0002 | 0.0045 | 0.0017 | 0.0076 | 0.0016 | 0.0008 | 0.0058 | 0.0042 | 0.0009 | 0.0048 | 0.0003 | 0.0009 | 0.0012 | 0.0063 | 0.0004 |
Vacuole | 0.0035 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0051 | 0.0004 | 0.0038 | 0.0021 | 0.0206 | 0.0006 | 0.0055 | 0 | 0.0002 | 0.0005 | 0.0003 | 0.0005 |
Vacuole Periphery | 0.0015 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0024 | 0.0001 | 0.0009 | 0.0011 | 0.0183 | 0.0005 | 0.0021 | 0 | 0.0001 | 0.0004 | 0.0004 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.9943 | 12.6155 | 14.9195 | 22.9602 | 12.2934 | 15.8675 | 22.7208 | 23.6751 | 22.5397 | 21.5236 |
Translational Efficiency | 0.9153 | 1.1573 | 0.9989 | 0.5855 | 1.0337 | 1.1701 | 0.709 | 0.7449 | 0.8326 | 0.7742 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2653 | 1226 | 166 | 1487 | 1731 | 1773 | 529 | 2171 | 4384 | 2999 | 695 | 3658 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 705.97 | 821.64 | 1079.34 | 966.86 | 719.01 | 808.97 | 883.75 | 994.81 | 711.12 | 814.15 | 930.47 | 983.45 |
Standard Deviation | 79.14 | 115.65 | 132.57 | 138.55 | 85.73 | 105.79 | 142.90 | 138.68 | 82.05 | 110.10 | 163.38 | 139.30 |
Intensity Change Log 2 | — | 0.218900 | 0.612471 | 0.453700 | — | 0.170074 | 0.297626 | 0.468409 | — | 0.194470 | 0.462185 | 0.461141 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000075 | 0.000609 | 0.001873 | 0.001582 | 0.000236 | 0.000305 | 0.000535 | 0.000958 | 0.000139 | 0.000429 | 0.000854 | 0.001212 |
Bud Neck | 0.003889 | 0.007620 | 0.007643 | 0.024024 | 0.003757 | 0.005266 | 0.004435 | 0.027232 | 0.003837 | 0.006228 | 0.005201 | 0.025928 |
Bud Site | 0.001203 | 0.002045 | 0.001331 | 0.004987 | 0.000458 | 0.001420 | 0.000865 | 0.004332 | 0.000909 | 0.001676 | 0.000976 | 0.004598 |
Cell Periphery | 0.000143 | 0.000071 | 0.000062 | 0.000070 | 0.000072 | 0.000120 | 0.000144 | 0.000217 | 0.000115 | 0.000100 | 0.000125 | 0.000157 |
Cytoplasm | 0.020249 | 0.004530 | 0.000961 | 0.003690 | 0.011244 | 0.004380 | 0.009842 | 0.004395 | 0.016693 | 0.004441 | 0.007720 | 0.004108 |
Cytoplasmic Foci | 0.001859 | 0.001689 | 0.000110 | 0.001777 | 0.001034 | 0.002116 | 0.001897 | 0.001110 | 0.001534 | 0.001942 | 0.001470 | 0.001381 |
Eisosomes | 0.000010 | 0.000024 | 0.000074 | 0.000029 | 0.000016 | 0.000021 | 0.000037 | 0.000026 | 0.000013 | 0.000022 | 0.000046 | 0.000027 |
Endoplasmic Reticulum | 0.001662 | 0.005277 | 0.010744 | 0.006464 | 0.002141 | 0.004708 | 0.003239 | 0.005636 | 0.001852 | 0.004940 | 0.005032 | 0.005973 |
Endosome | 0.000497 | 0.000983 | 0.000347 | 0.002037 | 0.000583 | 0.001411 | 0.000412 | 0.001745 | 0.000531 | 0.001236 | 0.000396 | 0.001863 |
Golgi | 0.000177 | 0.001162 | 0.000159 | 0.004467 | 0.000232 | 0.001516 | 0.000297 | 0.001938 | 0.000199 | 0.001372 | 0.000264 | 0.002966 |
Lipid Particles | 0.000489 | 0.001066 | 0.000445 | 0.000269 | 0.000826 | 0.000968 | 0.000444 | 0.000098 | 0.000622 | 0.001008 | 0.000444 | 0.000168 |
Mitochondria | 0.001341 | 0.003528 | 0.001458 | 0.002979 | 0.001572 | 0.005197 | 0.002110 | 0.003025 | 0.001432 | 0.004515 | 0.001955 | 0.003006 |
Mitotic Spindle | 0.001292 | 0.003743 | 0.000820 | 0.015552 | 0.003634 | 0.005449 | 0.003397 | 0.013594 | 0.002217 | 0.004751 | 0.002781 | 0.014390 |
None | 0.006423 | 0.005452 | 0.002143 | 0.003967 | 0.009775 | 0.004294 | 0.016635 | 0.003519 | 0.007746 | 0.004767 | 0.013174 | 0.003701 |
Nuclear Periphery | 0.001274 | 0.000556 | 0.001625 | 0.001613 | 0.000624 | 0.000968 | 0.000329 | 0.002185 | 0.001017 | 0.000799 | 0.000639 | 0.001952 |
Nuclear Periphery Foci | 0.000315 | 0.000932 | 0.000529 | 0.001059 | 0.001493 | 0.000426 | 0.000135 | 0.000675 | 0.000780 | 0.000632 | 0.000229 | 0.000831 |
Nucleolus | 0.005169 | 0.023079 | 0.025109 | 0.006907 | 0.022982 | 0.013683 | 0.013446 | 0.005386 | 0.012202 | 0.017524 | 0.016232 | 0.006004 |
Nucleus | 0.952078* | 0.934173* | 0.935639* | 0.911693* | 0.937913* | 0.943281* | 0.939996* | 0.920079* | 0.946485* | 0.939558* | 0.938955* | 0.916670* |
Peroxisomes | 0.000372 | 0.001221 | 0.000914 | 0.000375 | 0.000485 | 0.001202 | 0.000337 | 0.000208 | 0.000416 | 0.001210 | 0.000475 | 0.000276 |
Vacuole | 0.001319 | 0.001747 | 0.005850 | 0.005146 | 0.000575 | 0.002646 | 0.001310 | 0.002569 | 0.001025 | 0.002278 | 0.002394 | 0.003617 |
Vacuole Periphery | 0.000164 | 0.000494 | 0.002164 | 0.001314 | 0.000348 | 0.000625 | 0.000158 | 0.001074 | 0.000237 | 0.000572 | 0.000637 | 0.001172 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -9.95 | -10.67 | -3.71 | -2.18 | 2.96 | -1.26 | -7.69 | -4.64 | -4.29 | -1.64 | -8.87 | -13.24 | -5.86 | -4.16 | 0.16 |
Bud Neck | -3.54 | -4.60 | -17.26 | -12.43 | -11.92 | -2.29 | -2.63 | -22.59 | -20.57 | -16.39 | -4.08 | -3.89 | -28.17 | -23.49 | -19.77 |
Bud Site | -1.65 | -2.77 | -3.51 | -2.15 | -1.92 | -2.90 | -3.50 | -6.07 | -3.89 | -4.54 | -2.40 | -2.37 | -6.17 | -4.23 | -5.01 |
Cell Periphery | 2.93 | 2.23 | 2.81 | -0.56 | 1.23 | -2.39 | -2.98 | -1.46 | -0.57 | -0.32 | 0.84 | -0.35 | -0.17 | -0.63 | 0.04 |
Cytoplasm | 9.04 | 13.69 | 10.78 | 1.01 | -3.07 | 4.75 | 1.32 | 5.22 | 0.50 | 2.82 | 10.74 | 6.34 | 12.07 | 1.07 | 2.39 |
Cytoplasmic Foci | -0.04 | 4.42 | 1.99 | 1.67 | -2.42 | -0.85 | -0.15 | 1.98 | 2.79 | 1.52 | -0.54 | 0.83 | 3.00 | 3.21 | 1.07 |
Eisosomes | -9.14 | -11.69 | -18.25 | -5.12 | 8.39 | -2.13 | -10.16 | -7.24 | -3.87 | 5.40 | -7.87 | -15.85 | -17.76 | -6.07 | 8.88 |
Endoplasmic Reticulum | -11.47 | -9.29 | -15.83 | -3.31 | 5.42 | -6.30 | -6.29 | -13.24 | -3.11 | -5.21 | -11.72 | -11.06 | -21.29 | -4.43 | 0.52 |
Endosome | -1.85 | -0.50 | -2.61 | -0.65 | -2.39 | -2.02 | 0.94 | -1.25 | 0.72 | -2.20 | -2.93 | 0.70 | -2.62 | 0.28 | -3.18 |
Golgi | -2.24 | -0.38 | -2.47 | -1.19 | -2.44 | -2.37 | -0.07 | -1.80 | 0.55 | -1.71 | -3.21 | -0.28 | -3.08 | -0.42 | -2.92 |
Lipid Particles | -1.11 | -0.62 | 1.48 | 2.01 | 3.89 | -0.32 | 1.78 | 3.04 | 3.38 | 3.57 | -1.22 | 1.10 | 3.08 | 3.70 | 4.58 |
Mitochondria | -2.31 | -0.77 | -3.31 | 0.73 | -3.28 | -3.43 | -0.33 | -3.31 | 1.92 | -1.20 | -4.27 | -0.52 | -5.08 | 2.00 | -1.46 |
Mitotic Spindle | -2.00 | -1.32 | -5.12 | -3.62 | -2.23 | -1.07 | 0.35 | -3.73 | -2.72 | -3.37 | -2.51 | -0.78 | -6.72 | -4.43 | -4.21 |
None | 0.81 | 5.42 | 2.97 | 1.67 | -1.85 | 4.54 | -1.65 | 6.20 | 1.76 | 4.80 | 3.77 | -1.78 | 6.66 | 2.42 | 4.44 |
Nuclear Periphery | 3.51 | -0.25 | -1.65 | -5.44 | -0.36 | -1.62 | 1.89 | -7.15 | -5.03 | -8.68 | 1.32 | 1.66 | -5.62 | -6.95 | -6.29 |
Nuclear Periphery Foci | -2.59 | -1.99 | -1.32 | 1.14 | 0.49 | 2.25 | 2.71 | 2.12 | -0.43 | -2.18 | 0.52 | 2.08 | 1.09 | 0.77 | -1.44 |
Nucleolus | -9.77 | -4.93 | -3.64 | 7.66 | 3.83 | 5.03 | 5.19 | 10.24 | 5.30 | 3.24 | -4.37 | -1.22 | 5.80 | 9.15 | 4.56 |
Nucleus | 3.80 | 3.44 | 7.89 | 3.32 | 0.67 | -1.36 | -0.83 | 3.57 | 4.83 | 3.69 | 1.94 | 1.98 | 8.75 | 5.82 | 3.83 |
Peroxisomes | -2.54 | -3.80 | 0.77 | 2.85 | 4.48 | -2.30 | 1.58 | 2.93 | 3.89 | 1.69 | -3.57 | -0.43 | 2.69 | 4.80 | 3.39 |
Vacuole | -0.70 | -2.98 | -4.51 | -3.57 | 2.03 | -2.79 | -3.09 | -6.73 | -0.79 | -1.29 | -2.46 | -3.63 | -6.85 | -3.15 | 1.05 |
Vacuole Periphery | -2.27 | -1.06 | -3.26 | -1.30 | 0.51 | -1.53 | 1.46 | -1.60 | -0.26 | -2.87 | -2.98 | -0.78 | -3.57 | -0.99 | -0.85 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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