Standard name | |
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Human Ortholog | |
Description | RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 |
Bud | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 2 | 3 | 3 | 3 | 0 | 1 | 3 | 0 | 0 | 0 | 2 | 1 | 2 | 11 | 8 | 21 |
Bud Neck | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 2 | 2 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 6 |
Bud Site | 0 | 0 | 1 | 0 | 0 | 4 | 7 | 5 | 20 | 16 | 2 | 10 | 2 | 3 | 8 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 0 | 3 | 7 | 4 | 3 | 7 | 5 | 5 | 7 | 6 | 5 | 48 | 67 | 109 | 8 | 4 | 7 | 0 | 2 | 0 | 0 | 1 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 2 | 0 | 0 | 4 | 1 | 12 | 17 | 0 | 5 | 0 | 1 | 5 | 9 | 12 | 0 | 1 | 0 | 0 | 2 | 2 | 1 | 8 | 11 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 6 | 4 |
Mitochondria | 96 | 97 | 70 | 67 | 80 | 108 | 120 | 74 | 107 | 138 | 31 | 45 | 279 | 355 | 271 | 4 | 5 | 5 | 1 | 1 | 0 | 4 | 8 | 9 |
Nucleus | 41 | 75 | 53 | 19 | 21 | 23 | 32 | 40 | 24 | 42 | 6 | 14 | 124 | 53 | 61 | 8 | 12 | 14 | 27 | 53 | 26 | 44 | 54 | 109 |
Nuclear Periphery | 0 | 2 | 0 | 2 | 0 | 0 | 1 | 1 | 6 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 3 |
Nucleolus | 198 | 246 | 226 | 173 | 302 | 318 | 435 | 561 | 302 | 445 | 143 | 129 | 651 | 803 | 810 | 46 | 61 | 68 | 179 | 216 | 208 | 607 | 910 | 797 |
Peroxisomes | 0 | 0 | 2 | 1 | 2 | 3 | 6 | 8 | 8 | 5 | 1 | 1 | 0 | 7 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
SpindlePole | 0 | 0 | 1 | 5 | 15 | 18 | 62 | 89 | 43 | 93 | 30 | 21 | 5 | 21 | 37 | 0 | 0 | 0 | 4 | 5 | 6 | 12 | 50 | 28 |
Vac/Vac Membrane | 2 | 3 | 2 | 0 | 0 | 1 | 1 | 5 | 1 | 2 | 0 | 1 | 9 | 10 | 12 | 0 | 2 | 0 | 2 | 7 | 6 | 10 | 27 | 63 |
Unique Cell Count | 216 | 289 | 259 | 190 | 321 | 347 | 488 | 619 | 361 | 521 | 154 | 154 | 770 | 930 | 1006 | 59 | 75 | 84 | 221 | 293 | 256 | 713 | 1093 | 1073 |
Labelled Cell Count | 339 | 426 | 360 | 274 | 428 | 480 | 686 | 809 | 520 | 758 | 223 | 231 | 1124 | 1330 | 1334 | 67 | 86 | 94 | 221 | 293 | 256 | 713 | 1093 | 1073 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 25.1 | 21.8 | 21.8 | 22.1 | 17.8 | 13.6 | 11.3 | 10.4 | 9.6 | 8.7 | 8.1 | 7.7 | 32.5 | 29.3 | 26.7 | 35.8 | 39.3 | 47.3 | 31.7 | 28.6 | 30.8 |
Std Deviation (1e-4) | 9.8 | 7.6 | 7.6 | 7.8 | 5.2 | 3.6 | 3.4 | 2.9 | 3.6 | 2.8 | 2.8 | 3.6 | 12.9 | 12.6 | 12.5 | 20.5 | 20.0 | 24.2 | 10.3 | 9.1 | 10.0 |
Intensity Change (Log2) | – | – | – | 0.02 | -0.29 | -0.68 | -0.95 | -1.07 | -1.18 | -1.33 | -1.44 | -1.5 | 0.58 | 0.42 | 0.29 | 0.71 | 0.85 | 1.12 | 0.54 | 0.39 | 0.5 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 3.5 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 3.7 | 4.9 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.9 | -0.6 | 1.1 | -0.7 | -5.5 | 0.7 | -0.2 | -1.6 | 0.5 | 2.7 | 3.3 | 0 | -3.3 | -3.7 | -4.1 |
Nucleus | -3.0 | -5.0 | -5.1 | -5.7 | -6.1 | -5.1 | -5.0 | -4.7 | -3.0 | -1.6 | -7.4 | -7.2 | -1.2 | -0.9 | -0.8 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1.3 | 2.9 | 1.8 | 0.8 | 1.5 | -1.2 | -0.7 | 1.8 | -1.0 | -1.1 | -0.4 | -2.5 | -1.8 | -1.3 | -1.4 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 3.1 | 3.4 | 5.8 | 6.2 | 5.5 | 7.1 | 7.1 | 5.8 | 0 | 0 | 2.8 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 23.3487 | 32.9417 | 27.2932 | 29.537 | 28.1305 | 30.0018 | 24.9814 | 27.52 | 24.6668 | 23.149 | 19.3666 | 24.0098 | 23.426 | 31.6467 | 27.7307 | 26.364 | 21.8856 | 26.7924 |
Actin | 0.0056 | 0 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0077 | 0 | 0.011 | 0 | 0.0001 | 0 | 0.0192 | 0 | 0.0079 | 0 | 0.0001 | 0 |
Bud | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 |
Bud Neck | 0.0002 | 0 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0009 | 0 | 0 | 0.0002 | 0.0001 | 0 |
Bud Periphery | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0007 | 0 | 0.0001 | 0 | 0 | 0 |
Bud Site | 0.0007 | 0.0001 | 0.0012 | 0.0002 | 0.0002 | 0 | 0.0011 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0.0071 | 0 | 0.0002 | 0 | 0.0002 | 0 |
Cell Periphery | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 |
Cytoplasm | 0.0274 | 0.0005 | 0.0002 | 0 | 0 | 0 | 0.0009 | 0 | 0.0001 | 0 | 0 | 0 | 0.0022 | 0 | 0 | 0 | 0.0004 | 0 |
Cytoplasmic Foci | 0.0146 | 0 | 0.0051 | 0.0013 | 0.0008 | 0.0045 | 0.0192 | 0.0033 | 0.0087 | 0.0001 | 0.0113 | 0 | 0.023 | 0 | 0.0013 | 0.0001 | 0.0092 | 0 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0025 | 0 | 0.0001 | 0 | 0 | 0 | 0.0013 | 0 | 0.0002 | 0 | 0 | 0 | 0.0008 | 0 | 0 | 0 | 0.0002 | 0 |
Endosome | 0.009 | 0 | 0.0067 | 0 | 0 | 0 | 0.0305 | 0.0001 | 0.001 | 0 | 0.0005 | 0 | 0.0085 | 0 | 0.0004 | 0 | 0.0007 | 0 |
Golgi | 0.0017 | 0 | 0.0034 | 0.0001 | 0.0001 | 0 | 0.0085 | 0.0002 | 0.0021 | 0 | 0.001 | 0 | 0.0033 | 0 | 0.0016 | 0 | 0.0003 | 0 |
Lipid Particles | 0.0064 | 0 | 0.0108 | 0.0027 | 0.0051 | 0.0028 | 0.0784 | 0.0102 | 0.0092 | 0.0001 | 0.0571 | 0.0001 | 0.0165 | 0 | 0.0011 | 0.0001 | 0.0056 | 0.0001 |
Mitochondria | 0.0038 | 0.0007 | 0.0228 | 0.012 | 0.0116 | 0.0016 | 0.0039 | 0.0016 | 0.0017 | 0.0002 | 0.01 | 0.0002 | 0.0284 | 0.0004 | 0.0037 | 0.0002 | 0.0224 | 0.0003 |
None | 0.0112 | 0 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0047 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0083 | 0 | 0 | 0 | 0.0007 | 0 |
Nuclear Periphery | 0.0314 | 0.0022 | 0.0006 | 0.0004 | 0.0001 | 0 | 0.0101 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0.0195 | 0 | 0 | 0 | 0.001 | 0 |
Nucleolus | 0.7507 | 0.8292 | 0.8643 | 0.9008 | 0.9177 | 0.921 | 0.7747 | 0.8789 | 0.8696 | 0.9322 | 0.8565 | 0.9133 | 0.7494 | 0.8314 | 0.9091 | 0.9379 | 0.8952 | 0.9289 |
Nucleus | 0.0865 | 0.1534 | 0.0619 | 0.0789 | 0.0606 | 0.0687 | 0.0357 | 0.1028 | 0.0881 | 0.0671 | 0.0552 | 0.0861 | 0.0684 | 0.1677 | 0.0695 | 0.061 | 0.0435 | 0.0704 |
Peroxisomes | 0.0025 | 0 | 0.0017 | 0.0005 | 0.0007 | 0.0002 | 0.0064 | 0.0017 | 0.0003 | 0 | 0.0045 | 0 | 0.0042 | 0 | 0.0011 | 0 | 0.0007 | 0 |
Punctate Nuclear | 0.0426 | 0 | 0.014 | 0.0006 | 0.0016 | 0.0005 | 0.0092 | 0.0006 | 0.0062 | 0.0001 | 0.0019 | 0.0001 | 0.0313 | 0.0001 | 0.0028 | 0.0001 | 0.0182 | 0.0001 |
Vacuole | 0.001 | 0.0034 | 0.0034 | 0.0003 | 0.0003 | 0.0001 | 0.0023 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0 | 0.0032 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0 |
Vacuole Periphery | 0.0016 | 0.01 | 0.0018 | 0.0011 | 0.0004 | 0.0003 | 0.0043 | 0.0002 | 0.0003 | 0 | 0.0006 | 0 | 0.0041 | 0.0001 | 0.0007 | 0 | 0.0005 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 100.3733 | 99.7671 | 92.6557 | 65.7721 | 98.7414 | 55.4203 | 67.884 | 50.7414 | 45.9325 | 66.3498 |
Translational Efficiency | 1.5722 | 1.7089 | 1.4303 | 1.7188 | 1.3786 | 1.7995 | 1.5433 | 1.5068 | 1.3558 | 1.5317 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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