Standard name | |
---|---|
Human Ortholog | |
Description | Coiled-coil protein involved in spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of anaphase promoting complex activity; forms a complex with Mad2p; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 3 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 3 | 5 |
Bud | 0 | 1 | 2 | 0 | 1 | 5 | 5 | 7 | 5 | 7 | 9 | 10 | 4 | 0 | 2 | 0 | 1 | 1 | 2 | 1 | 1 | 2 | 3 | 4 |
Bud Neck | 0 | 0 | 5 | 0 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 2 |
Bud Site | 0 | 1 | 1 | 1 | 1 | 2 | 5 | 5 | 7 | 5 | 10 | 8 | 1 | 0 | 4 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 2 | 10 | 12 | 14 | 15 | 18 | 19 | 10 | 6 | 8 | 9 | 16 | 32 | 46 | 5 | 3 | 5 | 0 | 0 | 1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 1 | 4 | 3 | 3 | 2 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 5 | 2 | 4 | 3 | 0 | 0 | 0 | 2 | 0 | 2 | 2 |
Endosome | 24 | 16 | 30 | 8 | 27 | 32 | 27 | 23 | 1 | 3 | 4 | 19 | 33 | 69 | 64 | 42 | 21 | 18 | 29 | 41 | 66 | 22 | 72 | 86 |
Golgi | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 3 | 2 |
Mitochondria | 87 | 151 | 67 | 99 | 80 | 201 | 231 | 172 | 236 | 294 | 319 | 236 | 164 | 105 | 116 | 13 | 11 | 10 | 7 | 12 | 22 | 11 | 22 | 18 |
Nucleus | 7 | 10 | 17 | 24 | 15 | 22 | 50 | 53 | 28 | 29 | 48 | 43 | 17 | 5 | 7 | 4 | 1 | 2 | 3 | 3 | 2 | 1 | 2 | 0 |
Nuclear Periphery | 68 | 101 | 66 | 71 | 97 | 128 | 104 | 75 | 70 | 84 | 92 | 116 | 132 | 200 | 119 | 53 | 31 | 40 | 92 | 145 | 180 | 75 | 191 | 160 |
Nucleolus | 33 | 28 | 5 | 12 | 13 | 31 | 24 | 30 | 38 | 56 | 66 | 54 | 75 | 58 | 46 | 17 | 15 | 12 | 2 | 5 | 13 | 5 | 12 | 2 |
Peroxisomes | 0 | 2 | 1 | 1 | 3 | 5 | 7 | 2 | 2 | 3 | 0 | 2 | 2 | 7 | 5 | 0 | 0 | 2 | 2 | 5 | 5 | 1 | 20 | 19 |
SpindlePole | 2 | 8 | 26 | 10 | 14 | 18 | 23 | 44 | 21 | 18 | 18 | 39 | 9 | 9 | 14 | 6 | 4 | 2 | 14 | 18 | 14 | 7 | 30 | 27 |
Vac/Vac Membrane | 21 | 34 | 38 | 12 | 18 | 19 | 22 | 27 | 7 | 14 | 7 | 18 | 20 | 41 | 31 | 30 | 23 | 27 | 4 | 12 | 8 | 4 | 7 | 7 |
Unique Cell Count | 147 | 233 | 207 | 191 | 218 | 352 | 385 | 358 | 298 | 372 | 404 | 391 | 303 | 363 | 327 | 116 | 72 | 81 | 163 | 253 | 335 | 138 | 379 | 349 |
Labelled Cell Count | 249 | 359 | 275 | 254 | 289 | 482 | 519 | 460 | 425 | 519 | 582 | 557 | 475 | 532 | 457 | 176 | 114 | 120 | 163 | 253 | 335 | 138 | 379 | 349 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 4.7 | 5.6 | 5.1 | 5.7 | 4.6 | 4.8 | 5.1 | 4.1 | 4.0 | 3.9 | 4.6 | 5.3 | 5.6 | 5.6 | 8.5 | 8.4 | 8.2 | 5.2 | 5.2 | 5.5 |
Std Deviation (1e-4) | 0.8 | 0.8 | 1.1 | 1.1 | 1.4 | 1.0 | 1.3 | 1.0 | 1.0 | 0.9 | 0.8 | 1.0 | 1.0 | 1.3 | 1.5 | 1.8 | 1.9 | 1.6 | 1.2 | 1.1 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.14 | 0.04 | -0.27 | -0.21 | -0.12 | -0.43 | -0.46 | -0.51 | -0.27 | -0.07 | 0.0 | 0.0 | 0.61 | 0.6 | 0.56 | -0.09 | -0.09 | -0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 1.6 | 2.4 | 2.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.1114 | 1.402 | 1.1617 | 0.8538 | 1.3225 | 0.9803 | 1.6508 | 1.8126 | 1.9806 | 1.5488 | 1.9427 | 1.4406 | 1.8027 | 1.444 | 1.3574 | 1.5185 | 0.803 | 1.2461 |
Actin | 0.0321 | 0.0052 | 0.0063 | 0.0018 | 0.0335 | 0.0136 | 0.0353 | 0.0005 | 0.0109 | 0.0049 | 0.0013 | 0.0056 | 0.0551 | 0.002 | 0.0253 | 0.0018 | 0.001 | 0.0042 |
Bud | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0004 | 0.0002 | 0 | 0.0001 |
Bud Neck | 0.0137 | 0.0003 | 0.0002 | 0.0011 | 0.0007 | 0.0011 | 0.0004 | 0.0001 | 0.0005 | 0.0005 | 0.0005 | 0.0003 | 0.0004 | 0.0001 | 0.0024 | 0.0002 | 0.0001 | 0.001 |
Bud Periphery | 0.0006 | 0.0004 | 0.0002 | 0.0003 | 0.0003 | 0.0005 | 0.0009 | 0.0002 | 0.0008 | 0.0005 | 0.0003 | 0.0003 | 0.0012 | 0.0003 | 0.0014 | 0.0003 | 0.0002 | 0.0003 |
Bud Site | 0.0074 | 0.0008 | 0.0004 | 0.0015 | 0.0004 | 0.0004 | 0.0011 | 0.0001 | 0.0058 | 0.0006 | 0.0003 | 0.0004 | 0.0023 | 0.0004 | 0.0063 | 0.0009 | 0.0001 | 0.0001 |
Cell Periphery | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.0165 | 0.0012 | 0.0049 | 0.0005 | 0.0007 | 0.0005 | 0.0015 | 0.0001 | 0.0006 | 0.0003 | 0.0002 | 0.0005 | 0.0045 | 0.0002 | 0.0004 | 0.0023 | 0.0001 | 0.0003 |
Cytoplasmic Foci | 0.0378 | 0.0017 | 0.0058 | 0.0149 | 0.0189 | 0.002 | 0.0231 | 0.001 | 0.0075 | 0.01 | 0.0105 | 0.0018 | 0.0173 | 0.0009 | 0.0074 | 0.0024 | 0.0005 | 0.0005 |
Eisosomes | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.009 | 0.0011 | 0.0025 | 0.0016 | 0.0003 | 0.0037 | 0.0042 | 0.0008 | 0.0015 | 0.0027 | 0.0004 | 0.0011 | 0.0098 | 0.0014 | 0.0034 | 0.0012 | 0.0012 | 0.0021 |
Endosome | 0.0333 | 0.0024 | 0.0037 | 0.0071 | 0.0046 | 0.0158 | 0.0372 | 0.002 | 0.0127 | 0.0105 | 0.0467 | 0.0076 | 0.0213 | 0.0021 | 0.0028 | 0.0194 | 0.0016 | 0.0073 |
Golgi | 0.0129 | 0.0023 | 0.003 | 0.0033 | 0.0066 | 0.0048 | 0.022 | 0.0013 | 0.0108 | 0.0139 | 0.0305 | 0.0048 | 0.0108 | 0.0015 | 0.0044 | 0.002 | 0.0013 | 0.0033 |
Lipid Particles | 0.058 | 0.0376 | 0.0356 | 0.0552 | 0.0503 | 0.0282 | 0.1099 | 0.023 | 0.0469 | 0.0767 | 0.1968 | 0.0422 | 0.0625 | 0.0203 | 0.0249 | 0.0587 | 0.0304 | 0.0128 |
Mitochondria | 0.009 | 0.0042 | 0.0021 | 0.0075 | 0.0015 | 0.034 | 0.0206 | 0.0014 | 0.0127 | 0.0211 | 0.0086 | 0.0052 | 0.0075 | 0.002 | 0.0074 | 0.0023 | 0.0064 | 0.0147 |
None | 0.0098 | 0.0008 | 0.0141 | 0.0007 | 0.0006 | 0.0008 | 0.0019 | 0.0005 | 0.0013 | 0.0015 | 0.0003 | 0.0005 | 0.0072 | 0.0006 | 0.0008 | 0.0014 | 0.0007 | 0.0003 |
Nuclear Periphery | 0.634 | 0.7593 | 0.8404 | 0.7998 | 0.5951 | 0.7382 | 0.6549 | 0.8742 | 0.8041 | 0.7159 | 0.57 | 0.8671 | 0.7144 | 0.8519 | 0.7846 | 0.7675 | 0.8577 | 0.8697 |
Nucleolus | 0.0217 | 0.021 | 0.0069 | 0.0212 | 0.0313 | 0.0378 | 0.0038 | 0.0236 | 0.0088 | 0.034 | 0.0402 | 0.0149 | 0.0121 | 0.0286 | 0.027 | 0.013 | 0.0142 | 0.0207 |
Nucleus | 0.029 | 0.0944 | 0.0296 | 0.0471 | 0.0213 | 0.0555 | 0.0039 | 0.0219 | 0.0256 | 0.0104 | 0.0607 | 0.0139 | 0.0141 | 0.0262 | 0.0331 | 0.0287 | 0.0522 | 0.0335 |
Peroxisomes | 0.0172 | 0.0017 | 0.0069 | 0.0104 | 0.0529 | 0.003 | 0.0239 | 0.0004 | 0.0038 | 0.0272 | 0.0031 | 0.0008 | 0.0173 | 0.0007 | 0.0109 | 0.001 | 0.0005 | 0.0002 |
Punctate Nuclear | 0.0423 | 0.0483 | 0.0222 | 0.0141 | 0.1752 | 0.0475 | 0.0374 | 0.0282 | 0.0204 | 0.0321 | 0.0147 | 0.0221 | 0.0214 | 0.0423 | 0.039 | 0.0604 | 0.0123 | 0.0201 |
Vacuole | 0.0024 | 0.0012 | 0.0007 | 0.0035 | 0.0004 | 0.0015 | 0.0021 | 0.0009 | 0.0025 | 0.0018 | 0.0039 | 0.0006 | 0.002 | 0.0009 | 0.0009 | 0.0207 | 0.0007 | 0.0008 |
Vacuole Periphery | 0.0122 | 0.0158 | 0.0142 | 0.0079 | 0.0049 | 0.0108 | 0.0148 | 0.0197 | 0.0219 | 0.0349 | 0.0107 | 0.0101 | 0.0171 | 0.0174 | 0.0158 | 0.0154 | 0.0186 | 0.0081 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.4477 | 16.6588 | 19.1929 | 22.0071 | 18.7569 | 11.273 | 22.1628 | 21.8392 | 19.5984 | 24.8207 |
Translational Efficiency | 1.7591 | 1.3579 | 1.0443 | 0.9177 | 1.0999 | 1.8077 | 1.011 | 0.9403 | 1.0832 | 0.9146 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1962 | 301 | 136 | 1701 | 1986 | 1006 | 663 | 127 | 3948 | 1307 | 799 | 1828 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 648.09 | 842.23 | 1043.50 | 987.62 | 738.33 | 778.04 | 963.52 | 1068.38 | 693.48 | 792.82 | 977.13 | 993.23 |
Standard Deviation | 76.69 | 105.54 | 147.54 | 140.90 | 90.57 | 112.14 | 111.75 | 142.36 | 95.32 | 113.91 | 122.36 | 142.49 |
Intensity Change Log 2 | — | 0.378020 | 0.687165 | 0.607762 | — | 0.075579 | 0.384049 | 0.533087 | — | 0.224870 | 0.533691 | 0.568476 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000059 | 0.000121 | 0.000672 | 0.000138 | 0.000073 | 0.000240 | 0.000488 | 0.000274 | 0.000066 | 0.000212 | 0.000520 | 0.000147 |
Bud Neck | 0.000605 | 0.000118 | 0.002340 | 0.000813 | 0.000155 | 0.000360 | 0.000725 | 0.001378 | 0.000379 | 0.000304 | 0.001000 | 0.000853 |
Bud Site | 0.000223 | 0.000043 | 0.000365 | 0.000331 | 0.000050 | 0.000310 | 0.000189 | 0.000236 | 0.000136 | 0.000248 | 0.000219 | 0.000325 |
Cell Periphery | 0.000051 | 0.000020 | 0.000041 | 0.000019 | 0.000024 | 0.000057 | 0.000032 | 0.000021 | 0.000037 | 0.000049 | 0.000034 | 0.000019 |
Cytoplasm | 0.025257 | 0.000787 | 0.000272 | 0.000527 | 0.002349 | 0.002344 | 0.002320 | 0.000517 | 0.013734 | 0.001986 | 0.001971 | 0.000526 |
Cytoplasmic Foci | 0.022044 | 0.003508 | 0.000378 | 0.001754 | 0.003015 | 0.007439 | 0.001158 | 0.002938 | 0.012471 | 0.006533 | 0.001025 | 0.001836 |
Eisosomes | 0.000027 | 0.000006 | 0.000012 | 0.000003 | 0.000010 | 0.000018 | 0.000024 | 0.000006 | 0.000019 | 0.000016 | 0.000022 | 0.000003 |
Endoplasmic Reticulum | 0.002712 | 0.002918 | 0.006972 | 0.001655 | 0.002180 | 0.004071 | 0.004020 | 0.002587 | 0.002445 | 0.003806 | 0.004522 | 0.001720 |
Endosome | 0.006234 | 0.002256 | 0.001048 | 0.002202 | 0.002805 | 0.005520 | 0.001135 | 0.001677 | 0.004509 | 0.004768 | 0.001120 | 0.002166 |
Golgi | 0.003940 | 0.001631 | 0.000253 | 0.001075 | 0.001040 | 0.003774 | 0.000175 | 0.006457 | 0.002481 | 0.003281 | 0.000188 | 0.001449 |
Lipid Particles | 0.307720* | 0.068371 | 0.001669 | 0.001996 | 0.085858 | 0.120685* | 0.003182 | 0.002094 | 0.196115* | 0.108637* | 0.002924 | 0.002003 |
Mitochondria | 0.008471 | 0.001230 | 0.000869 | 0.001622 | 0.003522 | 0.005012 | 0.001335 | 0.001054 | 0.005981 | 0.004141 | 0.001255 | 0.001583 |
Mitotic Spindle | 0.022307 | 0.033533 | 0.151482* | 0.210071* | 0.014187 | 0.038852 | 0.135207* | 0.300502* | 0.018222 | 0.037627 | 0.137978* | 0.216353* |
None | 0.011727 | 0.000218 | 0.000268 | 0.000157 | 0.002035 | 0.002479 | 0.001953 | 0.000097 | 0.006852 | 0.001958 | 0.001666 | 0.000153 |
Nuclear Periphery | 0.031944 | 0.041064 | 0.195288* | 0.158808* | 0.039738 | 0.036314 | 0.115791* | 0.164045* | 0.035865 | 0.037408 | 0.129323* | 0.159172* |
Nuclear Periphery Foci | 0.512026* | 0.834531* | 0.536250* | 0.602844* | 0.813860* | 0.744674* | 0.576693* | 0.487044* | 0.663860* | 0.765368* | 0.569809* | 0.594799* |
Nucleolus | 0.023194 | 0.005022 | 0.041259 | 0.005446 | 0.017262 | 0.012859 | 0.067024 | 0.012870 | 0.020210 | 0.011054 | 0.062639 | 0.005962 |
Nucleus | 0.017562 | 0.003940 | 0.058684 | 0.006785 | 0.010492 | 0.011003 | 0.086405 | 0.012683 | 0.014006 | 0.009376 | 0.081687 | 0.007195 |
Peroxisomes | 0.002229 | 0.000372 | 0.000704 | 0.000098 | 0.000607 | 0.002444 | 0.000699 | 0.000467 | 0.001413 | 0.001967 | 0.000700 | 0.000124 |
Vacuole | 0.001152 | 0.000189 | 0.000769 | 0.002407 | 0.000409 | 0.000860 | 0.001252 | 0.001693 | 0.000778 | 0.000705 | 0.001170 | 0.002357 |
Vacuole Periphery | 0.000515 | 0.000124 | 0.000406 | 0.001249 | 0.000327 | 0.000684 | 0.000192 | 0.001361 | 0.000420 | 0.000555 | 0.000228 | 0.001257 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.24 | -5.05 | -2.54 | -0.76 | 4.11 | -4.87 | -9.02 | -3.19 | -1.67 | 1.75 | -5.46 | -10.36 | -3.01 | 0.92 | 7.36 |
Bud Neck | 5.96 | -2.88 | -1.92 | -7.38 | 2.56 | -3.34 | -8.60 | -2.43 | -2.20 | -1.44 | 1.31 | -5.95 | -4.91 | -5.57 | 1.98 |
Bud Site | 1.96 | -1.32 | -1.16 | -4.17 | 0.78 | -2.19 | -3.94 | -2.29 | -1.19 | -0.34 | -1.19 | -3.51 | -2.91 | -0.98 | 1.46 |
Cell Periphery | 6.06 | 1.29 | 5.81 | 0.24 | 2.99 | -2.34 | -4.90 | -0.97 | 1.60 | 1.75 | -0.97 | -1.93 | 3.91 | 2.51 | 4.67 |
Cytoplasm | 12.15 | 12.71 | 12.57 | 0.49 | -1.41 | -0.17 | -2.26 | -0.27 | -0.22 | 0.78 | 10.41 | 6.22 | 12.58 | 2.73 | 3.56 |
Cytoplasmic Foci | 8.44 | 15.08 | 13.90 | 0.88 | -3.10 | -3.41 | 1.58 | 0.39 | 2.31 | -0.33 | 6.00 | 11.98 | 12.97 | 4.80 | 0.12 |
Eisosomes | 12.65 | 7.86 | 18.37 | 3.01 | 5.42 | -3.53 | -6.84 | 0.32 | 2.12 | 5.41 | 2.45 | -4.18 | 18.16 | 7.91 | 9.24 |
Endoplasmic Reticulum | -0.52 | -3.50 | 3.60 | 1.93 | 4.21 | -5.06 | -6.41 | -1.53 | 2.28 | 3.33 | -4.38 | -6.92 | 2.97 | 5.87 | 8.12 |
Endosome | 5.13 | 9.00 | 6.68 | 0.06 | -2.93 | -3.13 | 1.55 | 0.82 | 3.43 | -0.26 | -0.38 | 6.32 | 5.21 | 3.91 | -1.01 |
Golgi | 2.47 | 7.67 | 5.38 | 0.58 | -2.80 | -3.27 | 5.69 | -0.86 | -0.39 | -1.01 | -0.90 | 8.91 | 2.27 | 2.31 | -2.41 |
Lipid Particles | 21.28* | 44.65* | 44.61* | 7.58 | -0.90 | -4.28 | 20.30 | 20.52 | 17.20 | 1.90 | 12.17 | 44.29 | 44.82 | 18.80 | 3.40 |
Mitochondria | 8.64 | 10.90 | 7.17 | -0.50 | -1.12 | -1.57 | 4.60 | 3.29 | 3.76 | 0.59 | 2.31 | 11.01 | 5.94 | 2.81 | -0.38 |
Mitotic Spindle | -1.50 | -6.43 | -24.71 | -17.26 | -2.33 | -6.25 | -13.03 | -9.31 | -8.44 | -4.94 | -5.56 | -14.21 | -27.23 | -22.49 | -6.17 |
None | 10.56 | 10.50 | 10.60 | 0.80 | 1.52 | -0.50 | -1.22 | 5.83 | 3.87 | 4.30 | 6.81 | 5.49 | 11.73 | 3.92 | 4.36 |
Nuclear Periphery | -2.40 | -9.33 | -30.72 | -21.44 | 1.87 | 1.42 | -11.52 | -7.31 | -7.52 | -3.08 | -0.97 | -15.06 | -31.41 | -29.41 | -5.88 |
Nuclear Periphery Foci | -22.12* | -1.21 | -9.62 | 15.82 | -2.94 | 6.24 | 19.51* | 11.91 | 9.16 | 2.34 | -11.02 | 9.36 | 7.13 | 16.08 | -4.05 |
Nucleolus | 8.58 | -1.61 | 10.49 | -0.13 | 4.31 | 2.02 | -9.73 | 0.44 | -0.36 | 7.23 | 5.87 | -9.27 | 10.96 | 3.54 | 13.02 |
Nucleus | 7.33 | -4.10 | 7.12 | -1.81 | 5.30 | -0.48 | -11.56 | -1.51 | -1.32 | 8.51 | 3.01 | -11.82 | 5.45 | 1.39 | 12.88 |
Peroxisomes | 6.79 | 5.62 | 8.25 | 2.64 | 4.32 | -3.17 | -2.84 | 0.10 | 3.03 | 1.89 | -0.87 | 2.79 | 9.29 | 4.20 | 7.31 |
Vacuole | 3.15 | 0.92 | -2.57 | -4.90 | -3.43 | -1.99 | -2.87 | -2.12 | -1.66 | 0.30 | -0.02 | -2.52 | -3.83 | -3.71 | 0.49 |
Vacuole Periphery | 4.03 | 0.74 | -2.44 | -3.76 | -2.73 | -1.77 | 1.37 | -0.99 | -0.62 | -1.10 | -0.93 | 2.57 | -2.91 | -2.18 | -3.50 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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