Standard name | |
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Human Ortholog | |
Description | Coiled-coil protein involved in spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of anaphase promoting complex activity; forms a complex with Mad2p; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 3 | 1 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 3 | 5 |
Bud | 0 | 1 | 2 | 0 | 1 | 5 | 5 | 7 | 5 | 7 | 9 | 10 | 4 | 0 | 2 | 0 | 1 | 1 | 2 | 1 | 1 | 2 | 3 | 4 |
Bud Neck | 0 | 0 | 5 | 0 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 2 |
Bud Site | 0 | 1 | 1 | 1 | 1 | 2 | 5 | 5 | 7 | 5 | 10 | 8 | 1 | 0 | 4 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 2 | 10 | 12 | 14 | 15 | 18 | 19 | 10 | 6 | 8 | 9 | 16 | 32 | 46 | 5 | 3 | 5 | 0 | 0 | 1 | 0 | 0 | 0 |
Endoplasmic Reticulum | 1 | 4 | 3 | 3 | 2 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 5 | 2 | 4 | 3 | 0 | 0 | 0 | 2 | 0 | 2 | 2 |
Endosome | 24 | 16 | 30 | 8 | 27 | 32 | 27 | 23 | 1 | 3 | 4 | 19 | 33 | 69 | 64 | 42 | 21 | 18 | 29 | 41 | 66 | 22 | 72 | 86 |
Golgi | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 2 | 0 | 3 | 2 |
Mitochondria | 87 | 151 | 67 | 99 | 80 | 201 | 231 | 172 | 236 | 294 | 319 | 236 | 164 | 105 | 116 | 13 | 11 | 10 | 7 | 12 | 22 | 11 | 22 | 18 |
Nucleus | 7 | 10 | 17 | 24 | 15 | 22 | 50 | 53 | 28 | 29 | 48 | 43 | 17 | 5 | 7 | 4 | 1 | 2 | 3 | 3 | 2 | 1 | 2 | 0 |
Nuclear Periphery | 68 | 101 | 66 | 71 | 97 | 128 | 104 | 75 | 70 | 84 | 92 | 116 | 132 | 200 | 119 | 53 | 31 | 40 | 92 | 145 | 180 | 75 | 191 | 160 |
Nucleolus | 33 | 28 | 5 | 12 | 13 | 31 | 24 | 30 | 38 | 56 | 66 | 54 | 75 | 58 | 46 | 17 | 15 | 12 | 2 | 5 | 13 | 5 | 12 | 2 |
Peroxisomes | 0 | 2 | 1 | 1 | 3 | 5 | 7 | 2 | 2 | 3 | 0 | 2 | 2 | 7 | 5 | 0 | 0 | 2 | 2 | 5 | 5 | 1 | 20 | 19 |
SpindlePole | 2 | 8 | 26 | 10 | 14 | 18 | 23 | 44 | 21 | 18 | 18 | 39 | 9 | 9 | 14 | 6 | 4 | 2 | 14 | 18 | 14 | 7 | 30 | 27 |
Vac/Vac Membrane | 21 | 34 | 38 | 12 | 18 | 19 | 22 | 27 | 7 | 14 | 7 | 18 | 20 | 41 | 31 | 30 | 23 | 27 | 4 | 12 | 8 | 4 | 7 | 7 |
Unique Cell Count | 147 | 233 | 207 | 191 | 218 | 352 | 385 | 358 | 298 | 372 | 404 | 391 | 303 | 363 | 327 | 116 | 72 | 81 | 163 | 253 | 335 | 138 | 379 | 349 |
Labelled Cell Count | 249 | 359 | 275 | 254 | 289 | 482 | 519 | 460 | 425 | 519 | 582 | 557 | 475 | 532 | 457 | 176 | 114 | 120 | 163 | 253 | 335 | 138 | 379 | 349 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 4.7 | 5.6 | 5.1 | 5.7 | 4.6 | 4.8 | 5.1 | 4.1 | 4.0 | 3.9 | 4.6 | 5.3 | 5.6 | 5.6 | 8.5 | 8.4 | 8.2 | 5.2 | 5.2 | 5.5 |
Std Deviation (1e-4) | 0.8 | 0.8 | 1.1 | 1.1 | 1.4 | 1.0 | 1.3 | 1.0 | 1.0 | 0.9 | 0.8 | 1.0 | 1.0 | 1.3 | 1.5 | 1.8 | 1.9 | 1.6 | 1.2 | 1.1 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.14 | 0.04 | -0.27 | -0.21 | -0.12 | -0.43 | -0.46 | -0.51 | -0.27 | -0.07 | 0.0 | 0.0 | 0.61 | 0.6 | 0.56 | -0.09 | -0.09 | -0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 1.6 | 2.4 | 2.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.1114 | 1.402 | 1.1617 | 0.8538 | 1.3225 | 0.9803 | 1.6508 | 1.8126 | 1.9806 | 1.5488 | 1.9427 | 1.4406 | 1.8027 | 1.444 | 1.3574 | 1.5185 | 0.803 | 1.2461 |
Actin | 0.0321 | 0.0052 | 0.0063 | 0.0018 | 0.0335 | 0.0136 | 0.0353 | 0.0005 | 0.0109 | 0.0049 | 0.0013 | 0.0056 | 0.0551 | 0.002 | 0.0253 | 0.0018 | 0.001 | 0.0042 |
Bud | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0004 | 0.0002 | 0 | 0.0001 |
Bud Neck | 0.0137 | 0.0003 | 0.0002 | 0.0011 | 0.0007 | 0.0011 | 0.0004 | 0.0001 | 0.0005 | 0.0005 | 0.0005 | 0.0003 | 0.0004 | 0.0001 | 0.0024 | 0.0002 | 0.0001 | 0.001 |
Bud Periphery | 0.0006 | 0.0004 | 0.0002 | 0.0003 | 0.0003 | 0.0005 | 0.0009 | 0.0002 | 0.0008 | 0.0005 | 0.0003 | 0.0003 | 0.0012 | 0.0003 | 0.0014 | 0.0003 | 0.0002 | 0.0003 |
Bud Site | 0.0074 | 0.0008 | 0.0004 | 0.0015 | 0.0004 | 0.0004 | 0.0011 | 0.0001 | 0.0058 | 0.0006 | 0.0003 | 0.0004 | 0.0023 | 0.0004 | 0.0063 | 0.0009 | 0.0001 | 0.0001 |
Cell Periphery | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0009 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.0165 | 0.0012 | 0.0049 | 0.0005 | 0.0007 | 0.0005 | 0.0015 | 0.0001 | 0.0006 | 0.0003 | 0.0002 | 0.0005 | 0.0045 | 0.0002 | 0.0004 | 0.0023 | 0.0001 | 0.0003 |
Cytoplasmic Foci | 0.0378 | 0.0017 | 0.0058 | 0.0149 | 0.0189 | 0.002 | 0.0231 | 0.001 | 0.0075 | 0.01 | 0.0105 | 0.0018 | 0.0173 | 0.0009 | 0.0074 | 0.0024 | 0.0005 | 0.0005 |
Eisosomes | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.009 | 0.0011 | 0.0025 | 0.0016 | 0.0003 | 0.0037 | 0.0042 | 0.0008 | 0.0015 | 0.0027 | 0.0004 | 0.0011 | 0.0098 | 0.0014 | 0.0034 | 0.0012 | 0.0012 | 0.0021 |
Endosome | 0.0333 | 0.0024 | 0.0037 | 0.0071 | 0.0046 | 0.0158 | 0.0372 | 0.002 | 0.0127 | 0.0105 | 0.0467 | 0.0076 | 0.0213 | 0.0021 | 0.0028 | 0.0194 | 0.0016 | 0.0073 |
Golgi | 0.0129 | 0.0023 | 0.003 | 0.0033 | 0.0066 | 0.0048 | 0.022 | 0.0013 | 0.0108 | 0.0139 | 0.0305 | 0.0048 | 0.0108 | 0.0015 | 0.0044 | 0.002 | 0.0013 | 0.0033 |
Lipid Particles | 0.058 | 0.0376 | 0.0356 | 0.0552 | 0.0503 | 0.0282 | 0.1099 | 0.023 | 0.0469 | 0.0767 | 0.1968 | 0.0422 | 0.0625 | 0.0203 | 0.0249 | 0.0587 | 0.0304 | 0.0128 |
Mitochondria | 0.009 | 0.0042 | 0.0021 | 0.0075 | 0.0015 | 0.034 | 0.0206 | 0.0014 | 0.0127 | 0.0211 | 0.0086 | 0.0052 | 0.0075 | 0.002 | 0.0074 | 0.0023 | 0.0064 | 0.0147 |
None | 0.0098 | 0.0008 | 0.0141 | 0.0007 | 0.0006 | 0.0008 | 0.0019 | 0.0005 | 0.0013 | 0.0015 | 0.0003 | 0.0005 | 0.0072 | 0.0006 | 0.0008 | 0.0014 | 0.0007 | 0.0003 |
Nuclear Periphery | 0.634 | 0.7593 | 0.8404 | 0.7998 | 0.5951 | 0.7382 | 0.6549 | 0.8742 | 0.8041 | 0.7159 | 0.57 | 0.8671 | 0.7144 | 0.8519 | 0.7846 | 0.7675 | 0.8577 | 0.8697 |
Nucleolus | 0.0217 | 0.021 | 0.0069 | 0.0212 | 0.0313 | 0.0378 | 0.0038 | 0.0236 | 0.0088 | 0.034 | 0.0402 | 0.0149 | 0.0121 | 0.0286 | 0.027 | 0.013 | 0.0142 | 0.0207 |
Nucleus | 0.029 | 0.0944 | 0.0296 | 0.0471 | 0.0213 | 0.0555 | 0.0039 | 0.0219 | 0.0256 | 0.0104 | 0.0607 | 0.0139 | 0.0141 | 0.0262 | 0.0331 | 0.0287 | 0.0522 | 0.0335 |
Peroxisomes | 0.0172 | 0.0017 | 0.0069 | 0.0104 | 0.0529 | 0.003 | 0.0239 | 0.0004 | 0.0038 | 0.0272 | 0.0031 | 0.0008 | 0.0173 | 0.0007 | 0.0109 | 0.001 | 0.0005 | 0.0002 |
Punctate Nuclear | 0.0423 | 0.0483 | 0.0222 | 0.0141 | 0.1752 | 0.0475 | 0.0374 | 0.0282 | 0.0204 | 0.0321 | 0.0147 | 0.0221 | 0.0214 | 0.0423 | 0.039 | 0.0604 | 0.0123 | 0.0201 |
Vacuole | 0.0024 | 0.0012 | 0.0007 | 0.0035 | 0.0004 | 0.0015 | 0.0021 | 0.0009 | 0.0025 | 0.0018 | 0.0039 | 0.0006 | 0.002 | 0.0009 | 0.0009 | 0.0207 | 0.0007 | 0.0008 |
Vacuole Periphery | 0.0122 | 0.0158 | 0.0142 | 0.0079 | 0.0049 | 0.0108 | 0.0148 | 0.0197 | 0.0219 | 0.0349 | 0.0107 | 0.0101 | 0.0171 | 0.0174 | 0.0158 | 0.0154 | 0.0186 | 0.0081 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.4477 | 16.6588 | 19.1929 | 22.0071 | 18.7569 | 11.273 | 22.1628 | 21.8392 | 19.5984 | 24.8207 |
Translational Efficiency | 1.7591 | 1.3579 | 1.0443 | 0.9177 | 1.0999 | 1.8077 | 1.011 | 0.9403 | 1.0832 | 0.9146 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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