Standard name
Human Ortholog
Description Component of the DNA ligase IV complex; this complex mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.88 0.93 0.89 0.87 0.84 0.71 0.62 0.54 0.44 0.46 0.34 0.47 0.92 0.96 0.96 0.54 0.52 0.59 0.5 0.8 0.44 0.83 0.79 0.77
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0.06 0.16 0.15 0.41 0.5 0.49 0.63 0.66 0.71 0.63 0 0 0 0 0 0 0.16 0 0.24 0 0 0
Nucleus 0 0 0 0 0 0 0.06 0.1 0.11 0.08 0.11 0.1 0 0 0 0.14 0.1 0.12 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0 0 0.17 0.05 0.07 0.07 0.05 0.07 0.05 0.06 0.09 0 0 0.05 0.06 0.4 0.47 0.37 0.09 0.07 0.12 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 4 0 2 5
Bud 1 0 2 1 0 8 12 6 8 9 14 14 0 0 0 0 1 0 5 0 4 0 11 15
Bud Neck 2 0 0 0 0 0 1 0 0 2 0 1 4 2 0 0 0 1 1 0 1 1 2 2
Bud Site 0 0 0 0 0 1 1 3 3 4 7 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 2 0 0 0 0 0 0 0 1 2 1 0 2 0 0 0 1 0 0 0 1 2
Cytoplasm 204 157 204 170 263 271 364 278 200 223 150 236 195 384 413 113 68 59 187 154 108 186 338 342
Endoplasmic Reticulum 1 0 0 0 0 0 1 1 1 1 0 0 0 0 2 7 5 3 30 0 3 0 0 0
Endosome 2 1 3 3 6 2 5 13 3 0 2 2 1 0 3 11 8 1 7 0 5 2 1 6
Golgi 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 4 0 2 0 1 0
Mitochondria 13 5 13 32 46 156 295 250 287 320 318 319 0 2 0 4 2 1 59 6 59 6 14 17
Nucleus 7 3 5 1 5 13 35 49 52 37 49 51 6 5 4 29 13 12 10 7 9 6 6 3
Nuclear Periphery 1 0 0 0 0 0 1 0 2 2 4 2 0 0 0 0 0 0 0 0 1 0 1 1
Nucleolus 0 0 0 0 1 1 3 2 3 1 2 0 0 0 0 0 0 0 2 0 1 0 0 0
Peroxisomes 0 0 0 0 1 1 0 1 1 1 0 0 0 0 0 0 0 0 1 1 1 0 0 0
SpindlePole 3 0 2 2 2 2 2 3 6 4 5 6 1 0 0 0 0 0 10 2 3 4 12 20
Vac/Vac Membrane 4 6 38 9 23 26 32 36 22 29 38 22 9 21 24 84 61 37 32 13 29 7 15 16
Unique Cell Count 232 169 230 195 312 383 587 512 459 488 445 505 211 401 430 210 130 100 373 192 245 225 428 447
Labelled Cell Count 239 173 270 218 348 481 752 642 588 633 590 658 217 414 448 248 160 114 373 192 245 225 428 447


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.0 4.3 4.0 3.8 3.4 3.2 3.3 3.2 2.9 2.8 2.7 5.2 5.3 5.2 5.9 6.0 5.9 5.5 5.6 5.6
Std Deviation (1e-4) 0.8 0.7 1.7 2.2 1.7 1.6 1.4 1.3 1.6 1.1 1.1 0.8 1.0 1.1 1.2 1.0 1.3 1.1 1.7 1.6 1.6
Intensity Change (Log2) – – – -0.11 -0.18 -0.37 -0.45 -0.39 -0.44 -0.56 -0.62 -0.66 0.27 0.29 0.27 0.43 0.47 0.45 0.35 0.36 0.36


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 5.5 6.2 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0367 0.517 0.6607 0.0848 0.0728 0.5126 -0.2859 -0.8336 -0.7157 -0.721 -0.1637 -0.6943 -0.6395 -1.0767 -1.2578 -1.2774 -1.0887 -1.3555
Actin 0.0003 0.0002 0.0003 0.0002 0.0006 0.0005 0.0881 0.0009 0.0166 0.0442 0.0914 0.0112 0.0391 0.0009 0.0155 0.0015 0.0193 0.0004
Bud 0.0003 0.0126 0.0025 0.0001 0.0078 0.0003 0.0006 0.0014 0.0011 0.0011 0.0021 0.0013 0.0021 0.0006 0.0025 0.0001 0.0004 0.0019
Bud Neck 0.0001 0.0001 0.0003 0.0007 0.0001 0.0005 0.0052 0.0005 0.0052 0.0009 0.0024 0.0013 0.0011 0.0002 0.0006 0.0008 0.0005 0.0011
Bud Periphery 0.0007 0.0019 0.0033 0.0001 0.0086 0.0005 0.0009 0.0006 0.0009 0.0014 0.0061 0.0017 0.0025 0.0006 0.0009 0.0001 0.0006 0.0006
Bud Site 0.0002 0.0067 0.0011 0.0007 0.0001 0 0.0038 0.0099 0.0099 0.0009 0.0067 0.0004 0.0021 0.0015 0.0032 0.0008 0.0013 0.0002
Cell Periphery 0.0001 0.0002 0.0001 0.0001 0.0001 0 0.0003 0.0001 0.0009 0.0001 0.0005 0.0001 0.0007 0.0001 0.0003 0 0.0001 0.0001
Cytoplasm 0.1 0.1593 0.1024 0.1879 0.1583 0.218 0.1221 0.2251 0.1757 0.1531 0.1587 0.355 0.1858 0.3016 0.2316 0.2428 0.1697 0.3119
Cytoplasmic Foci 0.0041 0.0025 0.0027 0.0029 0.0057 0.0095 0.0262 0.0044 0.0167 0.0094 0.031 0.0193 0.0374 0.0066 0.0119 0.013 0.0055 0.0273
Eisosomes 0.0004 0.0001 0.0003 0.0001 0.0001 0 0.0007 0.0001 0.0002 0.0005 0.0002 0.0001 0.0006 0.0001 0.0001 0.0001 0.0002 0
Endoplasmic Reticulum 0.002 0.0027 0.0016 0.0012 0.0007 0.0013 0.0112 0.0023 0.0038 0.0022 0.0025 0.0054 0.0061 0.0022 0.0034 0.0016 0.0042 0.0017
Endosome 0.0011 0.0005 0.0009 0.0013 0.0003 0.0012 0.0427 0.002 0.0081 0.0081 0.0189 0.0166 0.0264 0.0019 0.0071 0.0064 0.0233 0.0044
Golgi 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0073 0.0001 0.0034 0.0036 0.0113 0.0055 0.0055 0.0001 0.0015 0.001 0.005 0.0002
Lipid Particles 0.0004 0.0002 0.0002 0.0001 0.0002 0.0002 0.0072 0.0007 0.0055 0.0047 0.0273 0.0046 0.0122 0.0002 0.0012 0.0035 0.017 0.0008
Mitochondria 0.0022 0.0004 0.002 0.0003 0.0003 0.0006 0.0037 0.0003 0.0075 0.0017 0.0098 0.0052 0.0089 0.0007 0.0019 0.0008 0.0051 0.0012
None 0.8818 0.7854 0.8228 0.7436 0.8131 0.7371 0.5611 0.7238 0.7097 0.7071 0.5603 0.5008 0.577 0.6293 0.6429 0.6741 0.6021 0.5758
Nuclear Periphery 0.001 0.0017 0.005 0.0015 0.0007 0.0029 0.0326 0.0051 0.0029 0.0082 0.0039 0.0085 0.0188 0.0032 0.0087 0.0028 0.0191 0.0029
Nucleolus 0.0003 0.0004 0.0007 0.0005 0.0001 0.0003 0.0025 0.001 0.0005 0.0023 0.0027 0.0006 0.0086 0.0005 0.0016 0.001 0.001 0.0017
Nucleus 0.003 0.0231 0.0484 0.0548 0.0013 0.0201 0.0455 0.0147 0.0194 0.0202 0.0026 0.038 0.0332 0.0423 0.0471 0.0366 0.1139 0.0488
Peroxisomes 0.0002 0.0001 0.0002 0.0001 0.0002 0.0003 0.0115 0.0002 0.0062 0.0042 0.0506 0.005 0.0057 0.0002 0.0005 0.0025 0.0016 0.0003
Punctate Nuclear 0.0013 0.0012 0.0042 0.0023 0.0015 0.0059 0.016 0.0051 0.004 0.0249 0.0043 0.0168 0.0175 0.0059 0.0154 0.0092 0.0067 0.0175
Vacuole 0.0006 0.0006 0.0008 0.0014 0.0004 0.0004 0.0081 0.0011 0.0015 0.0009 0.0054 0.0014 0.0058 0.0011 0.0015 0.001 0.0019 0.001
Vacuole Periphery 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0.0028 0.0003 0.0004 0.0004 0.0013 0.0011 0.0029 0.0002 0.0006 0.0002 0.0017 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.4863 4.6748 5.2033 6.5383 2.2601 5.2265 6.4991 6.6462 10.269 8.7577
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1476 648 1431 1468 1614 1489 514 204 3090 2137 1945 1672

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 593.01 759.87 803.96 771.54 649.93 699.18 838.04 872.18 622.74 717.58 812.97 783.82
Standard Deviation 65.41 90.60 92.95 111.25 73.63 84.62 105.30 109.09 75.39 90.87 97.53 115.77
Intensity Change Log 2 — 0.357696 0.439067 0.379685 — 0.105380 0.366735 0.424342 — 0.231273 0.401697 0.403208

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000226 0.000338 0.000239 0.000500 0.000070 0.000324 0.000218 0.000562 0.000145 0.000328 0.000233 0.000507
Bud Neck 0.023449 0.026515 0.006176 0.008275 0.008985 0.038869 0.003020 0.004160 0.015894 0.035123 0.005342 0.007773
Bud Site 0.004062 0.010751 0.004953 0.015006 0.003574 0.013030 0.001923 0.011811 0.003807 0.012339 0.004152 0.014616
Cell Periphery 0.000194 0.000097 0.000134 0.000286 0.000173 0.000165 0.000117 0.000195 0.000183 0.000144 0.000129 0.000275
Cytoplasm 0.381052* 0.364237* 0.154855* 0.384821* 0.492417* 0.357595* 0.139618* 0.277344* 0.439222* 0.359609* 0.150828* 0.371708*
Cytoplasmic Foci 0.247149* 0.171473 0.003026 0.016405 0.140533 0.207626* 0.003923 0.018248 0.191460* 0.196663* 0.003263 0.016629
Eisosomes 0.000474 0.000090 0.000027 0.000043 0.000109 0.000150 0.000037 0.000084 0.000284 0.000132 0.000029 0.000048
Endoplasmic Reticulum 0.000897 0.001210 0.003694 0.003809 0.001210 0.000722 0.004213 0.006935 0.001060 0.000870 0.003832 0.004191
Endosome 0.009164 0.015232 0.001282 0.003789 0.004333 0.018306 0.000937 0.003297 0.006641 0.017374 0.001191 0.003729
Golgi 0.003360 0.003282 0.000278 0.004925 0.000750 0.005393 0.000033 0.000085 0.001997 0.004753 0.000213 0.004334
Lipid Particles 0.015193 0.005430 0.000800 0.001333 0.006415 0.009523 0.001113 0.002776 0.010608 0.008282 0.000883 0.001509
Mitochondria 0.010473 0.007816 0.000618 0.003505 0.001808 0.019420 0.000525 0.000469 0.005947 0.015901 0.000593 0.003135
Mitotic Spindle 0.001267 0.019925 0.003421 0.014135 0.001391 0.014478 0.000621 0.009659 0.001332 0.016130 0.002681 0.013589
None 0.004892 0.003100 0.004155 0.005041 0.005059 0.002617 0.004468 0.007237 0.004979 0.002764 0.004238 0.005309
Nuclear Periphery 0.000691 0.000899 0.001910 0.002137 0.000691 0.000810 0.001701 0.001674 0.000691 0.000837 0.001855 0.002081
Nuclear Periphery Foci 0.000294 0.000404 0.000459 0.001302 0.000339 0.000337 0.000595 0.001821 0.000317 0.000357 0.000495 0.001366
Nucleolus 0.002164 0.002642 0.000545 0.000398 0.001474 0.003944 0.000556 0.000663 0.001803 0.003549 0.000548 0.000430
Nucleus 0.251526* 0.272525* 0.795929* 0.456976* 0.300779* 0.218131* 0.809427* 0.456856* 0.277252* 0.234625* 0.799496* 0.456961*
Peroxisomes 0.010398 0.019733 0.000149 0.000456 0.002608 0.017835 0.000131 0.000510 0.006329 0.018410 0.000144 0.000463
Vacuole 0.032088 0.072286 0.017226 0.074923 0.026778 0.067759 0.026722 0.195450 0.029314 0.069132 0.019735 0.089628
Vacuole Periphery 0.000985 0.002015 0.000124 0.001934 0.000504 0.002965 0.000102 0.000162 0.000734 0.002677 0.000118 0.001718

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.33 -1.00 -2.10 -1.28 -2.01 -2.92 -8.59 -5.52 -2.57 -2.98 -2.50 -3.66 -4.54 -1.85 -2.57
Bud Neck -0.88 7.55 7.06 6.15 -0.96 -11.04 4.09 3.39 13.00 -1.47 -8.66 7.39 5.89 12.72 -1.55
Bud Site -4.60 -0.54 -3.80 0.10 -3.08 -5.47 1.43 -1.62 1.73 -2.23 -6.83 -0.25 -4.26 1.15 -3.83
Cell Periphery 6.84 3.62 -0.45 -3.14 -1.97 0.78 2.89 0.48 -0.03 -1.74 3.94 4.53 -0.69 -1.94 -2.17
Cytoplasm 2.02 28.14 7.70 3.79 -18.92 14.61 36.80 19.54 10.69 -3.75 11.11 47.26 18.49 7.40 -19.88
Cytoplasmic Foci 8.27 42.93 40.58 21.32 -10.27 -9.97 34.00 30.02 35.11 -5.35 -0.80 53.01 48.92 41.45 -11.03
Eisosomes 1.56 1.80 1.70 6.53 -8.75 -2.95 8.44 -0.11 2.61 -6.14 1.29 2.09 1.87 7.27 -9.31
Endoplasmic Reticulum -2.05 -16.25 -16.75 -14.62 -1.57 1.40 -9.56 -6.75 -7.56 -1.50 0.77 -16.31 -15.83 -19.15 -1.49
Endosome -3.68 10.05 7.96 8.37 -2.97 -11.57 8.32 2.53 12.57 -5.25 -10.70 12.24 7.79 15.05 -3.74
Golgi 0.25 5.49 1.06 0.58 -2.33 -5.64 5.74 5.19 6.53 -5.63 -3.76 6.14 -0.02 2.61 -2.38
Lipid Particles 8.12 13.11 12.58 6.93 -4.88 -3.21 8.33 5.81 8.41 -4.05 2.92 15.63 14.62 11.62 -5.41
Mitochondria 1.80 7.95 5.83 3.76 -1.88 -9.25 4.71 4.68 10.00 -0.00 -6.52 8.71 4.54 9.13 -1.91
Mitotic Spindle -4.35 -2.53 -4.35 2.33 -2.24 -5.39 -0.26 -2.48 -0.64 -2.42 -6.98 -2.46 -5.01 1.99 -3.35
None 2.60 1.92 1.33 -1.84 -0.83 3.88 1.33 0.50 -1.86 -0.41 4.86 2.47 1.65 -3.65 -0.84
Nuclear Periphery -2.29 -13.95 -14.65 -13.19 -4.92 -0.88 -8.78 -9.59 -10.90 -2.38 -1.80 -15.28 -15.77 -16.13 -5.42
Nuclear Periphery Foci -2.62 -5.76 -8.93 -7.29 -5.55 -0.04 -4.01 -6.93 -6.94 -4.42 -1.47 -6.70 -10.29 -9.74 -6.31
Nucleolus -1.27 4.51 4.92 5.02 2.62 -3.62 2.84 1.58 4.95 -0.89 -3.63 5.17 5.50 7.75 1.57
Nucleus -1.84 -61.94 -23.40 -15.79 31.51 9.72 -40.39 -5.58 -9.09 14.50 6.08 -74.95 -23.42 -26.48 34.99
Peroxisomes -3.55 12.82 12.53 7.89 -2.33 -9.81 6.16 5.64 11.74 -4.04 -8.89 14.01 13.43 14.13 -2.71
Vacuole -9.57 1.26 -20.13 -8.90 -20.34 -12.96 -5.57 -12.98 -10.99 -11.05 -15.77 -3.34 -23.97 -15.16 -21.42
Vacuole Periphery -2.47 9.04 0.07 1.90 -2.44 -6.08 5.68 5.71 7.12 -0.36 -6.27 10.56 -0.41 4.19 -2.45
Standard name
Human Ortholog
Description Component of the DNA ligase IV complex; this complex mediates nonhomologous end joining in DNA double-strand break repair; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Lif1

Lif1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lif1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available