Standard name
Human Ortholog
Description Vacuolar alpha mannosidase; involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0.05 0.06 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm 0.96 0.96 0.96 0.96 0.32 0.31 0.37 0.4 – 0.44 0.49 0.47 0.95 0.96 0.95 – – 0.61 0.82 0.84 0.65 0.71 0.75 0.72
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.38 0.3 0.24 0.14 – 0.1 0.09 0.1 0 0 0 – – 0.15 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0.08 0 0.07 0 0.07 – 0.11 0.08 0 0 0 0 – – 0 0 0 0.13 0.12 0.1 0.07
Nucleus 0 0 0 0 0 0 0 0.05 – 0.06 0.06 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.13 0.13 0.09 – 0.06 0.07 0.05 0 0 0 – – 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.11 0 0 0 – 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.41 0.23 0.17 0.1 – 0.06 0.1 0.06 0 0 0 – – 0.16 0 0 0 0 0 0.05
Vac/Vac Membrane 0 0 0 0 0.1 0.26 0.38 0.41 – 0.42 0.38 0.49 0 0 0 – – 0.27 0 0 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 – 1 0 1 1 2 1 – – 1 2 4 6 5 6 4
Bud 1 3 1 4 14 22 20 22 – 9 13 14 0 0 0 – – 4 7 4 3 5 7 12
Bud Neck 2 0 1 0 4 1 0 0 – 1 0 0 2 1 0 – – 0 1 2 2 0 4 3
Bud Site 0 0 1 0 2 5 2 3 – 3 3 2 0 0 0 – – 2 – – – – – –
Cell Periphery 2 2 1 0 1 0 0 0 – 1 2 0 0 0 0 – – 0 2 0 2 3 2 2
Cytoplasm 394 318 322 235 87 124 201 216 – 215 168 263 220 220 205 – – 230 336 283 216 186 255 223
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 – 1 0 0 6 6 3 – – 10 0 0 5 1 2 1
Endosome 1 2 4 4 102 121 129 73 – 48 30 54 2 2 2 – – 58 10 5 14 4 5 3
Golgi 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – 1 2 1 2 0 0 2
Mitochondria 7 18 13 20 4 29 18 35 – 55 26 18 0 0 0 – – 13 11 12 44 32 32 22
Nucleus 0 0 0 0 2 7 8 29 – 29 19 7 2 2 2 – – 4 0 1 0 1 0 0
Nuclear Periphery 0 0 2 0 0 0 0 0 – 1 1 2 0 0 0 – – 0 0 0 0 1 0 0
Nucleolus 0 0 1 0 12 50 69 47 – 29 23 28 0 0 0 – – 18 0 0 0 0 0 0
Peroxisomes 1 0 2 0 29 14 6 3 – 4 4 4 0 0 0 – – 5 0 0 0 0 0 0
SpindlePole 7 1 8 5 110 93 92 53 – 31 33 34 2 1 4 – – 61 14 6 14 5 6 14
Vac/Vac Membrane 1 2 5 0 26 102 205 221 – 201 128 272 0 0 0 – – 101 9 7 15 7 7 12
Unique Cell Count 410 331 336 246 269 398 543 537 484 340 559 232 230 215 377 408 338 335 262 339 312
Labelled Cell Count 417 347 361 268 393 569 750 702 629 450 699 235 234 217 508 408 338 335 262 339 312


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 3.7 3.5 3.3 4.8 5.4 6.1 6.2 – 6.1 6.5 7.5 5.1 5.1 5.2 4.7 4.7 6.6 5.0 5.1 5.6
Std Deviation (1e-4) 0.6 0.9 1.6 1.3 1.8 1.7 1.9 2.0 – 2.1 2.4 2.9 1.2 1.2 1.1 1.7 1.2 2.8 1.9 1.6 2.2
Intensity Change (Log2) – – – -0.08 0.46 0.63 0.8 0.83 – 0.79 0.89 1.1 0.55 0.55 0.58 0.44 0.42 0.93 0.52 0.54 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – 0
Bud Neck – – – – – – – – – – – – – – 0
Bud Site – – – – – – – – – – – – – – 0
Cell Periphery – – – – – – – – – – – – – – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – 0
Endosome – – – – – – – – – – – – – – 0
Golgi – – – – – – – – – – – – – – 0
Mitochondria – – – – – – – – – – – – – – 0
Nuclear Periphery – – – – – – – – – – – – – – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – 0
Peroxisomes – – – – – – – – – – – – – – 0
SpindlePole – – – – – – – – – – – – – – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – 0
Cytoplasm – – – – – – – – – – – – – – 0
Nucleus – – – – – – – – – – – – – – 0
Vacuole – – – – – – – – – – – – – – 9.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0269 0.0058 0.0097 0.0032 0.0048 0.0023 0.032 0.0001 0.0301 0.0343 0.0241 0.0081 0.0001 0.0001 0.0001 0 0 0.0011
Bud 0.0024 0.0068 0.0016 0.0005 0.0005 0.0006 0.0023 0.0032 0.0025 0.0002 0.0014 0.0004 0 0 0 0 0 0.0001
Bud Neck 0.0095 0.0039 0.0017 0.0007 0.0003 0.0011 0.0026 0.0001 0.0009 0.0152 0.0006 0.0004 0 0 0 0 0 0.0001
Bud Periphery 0.0055 0.01 0.0018 0.0008 0.0012 0.0013 0.0096 0.0091 0.0096 0.0002 0.0035 0.001 0 0 0 0 0 0.0001
Bud Site 0.0087 0.0246 0.0086 0.0006 0.0022 0.0005 0.0062 0.0016 0.01 0.0016 0.0025 0.0008 0 0 0 0 0 0.0003
Cell Periphery 0.0014 0.0011 0.0003 0.0001 0.0002 0.0001 0.0005 0.0003 0.0005 0.0002 0.0002 0.0001 0 0.0001 0 0 0 0
Cytoplasm 0.0431 0.0922 0.0648 0.0612 0.0454 0.0973 0.0885 0.0772 0.0526 0.0625 0.0879 0.1523 0.0281 0.0957 0.0703 0.2525 0.0051 0.0553
Cytoplasmic Foci 0.0451 0.0305 0.0201 0.0849 0.0728 0.0386 0.0874 0.0027 0.0646 0.048 0.0121 0.03 0.0007 0.0164 0.0009 0.0046 0.0004 0.0655
Eisosomes 0.0003 0.0002 0.0003 0.0001 0.0004 0.0001 0.0006 0.0003 0.0004 0.0003 0.0002 0.0001 0.0002 0.0001 0.0001 0 0 0
Endoplasmic Reticulum 0.0044 0.0015 0.0016 0.0011 0.0024 0.0021 0.0085 0.0005 0.0028 0.0003 0.0009 0.0017 0.0008 0.0021 0.0005 0.0011 0 0.0004
Endosome 0.0305 0.0154 0.0113 0.0194 0.0219 0.0133 0.0315 0.0005 0.0213 0.0015 0.0127 0.0081 0.0001 0.0108 0.0001 0.0042 0 0.0154
Golgi 0.0088 0.0012 0.0035 0.0073 0.0029 0.0045 0.0082 0.0001 0.0073 0.0047 0.0168 0.0018 0 0.0002 0 0 0 0.009
Lipid Particles 0.0177 0.0041 0.0062 0.0098 0.0134 0.0052 0.0108 0.0002 0.0136 0.0024 0.0029 0.0126 0.0001 0.0009 0.0001 0 0 0.0046
Mitochondria 0.0073 0.0028 0.0077 0.0068 0.0135 0.008 0.0046 0.0014 0.0192 0.0003 0.0081 0.0045 0 0.0006 0 0.0001 0 0.003
None 0.7255 0.7533 0.8278 0.7683 0.7157 0.7974 0.6693 0.8991 0.7345 0.7971 0.7857 0.7363 0.9693 0.8652 0.9273 0.7338 0.9943 0.8352
Nuclear Periphery 0.022 0.0025 0.0016 0.0018 0.0417 0.0023 0.0097 0.0003 0.0052 0.0001 0.0097 0.0041 0.0001 0.0008 0.0001 0.0005 0 0.0002
Nucleolus 0.0023 0.0022 0.0007 0.0005 0.001 0.0006 0.0007 0.0005 0.0013 0.0001 0.0068 0.0002 0 0.0001 0 0 0 0
Nucleus 0.0123 0.0043 0.0083 0.001 0.0272 0.0034 0.0061 0.001 0.0013 0.0002 0.0108 0.0037 0.0001 0.0006 0.0001 0.0004 0 0.0001
Peroxisomes 0.0071 0.0115 0.0026 0.0162 0.0079 0.0066 0.0108 0.0002 0.0151 0.0047 0.0049 0.0203 0 0.0001 0 0 0 0.008
Punctate Nuclear 0.0072 0.0073 0.0125 0.0122 0.0025 0.0097 0.004 0.0006 0.0024 0.0261 0.005 0.0019 0.0001 0.0003 0.0002 0.0001 0 0.0014
Vacuole 0.0089 0.0163 0.0066 0.0028 0.0202 0.0045 0.0049 0.001 0.0035 0.0002 0.002 0.0109 0.0001 0.0051 0.0001 0.002 0 0.0002
Vacuole Periphery 0.0033 0.0025 0.0007 0.0007 0.002 0.0008 0.0012 0.0002 0.0012 0 0.0014 0.0008 0 0.0006 0 0.0005 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.1432 4.853 2.9259 5.0297 3.2994 7.7996 7.8203 10.6599 11.1828 11.4006
Translational Efficiency 0.6936 0.6445 1.0037 0.687 0.9677 1.1041 0.6441 0.3405 0.6574 0.6108

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1052 1518 411 398 600 1737 2879 1926 1652 3255 3290 2324

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 740.12 729.30 918.17 875.90 668.42 709.94 750.60 774.82 714.08 718.97 771.53 792.13
Standard Deviation 109.66 91.71 122.35 215.03 98.75 81.67 84.79 115.47 111.31 87.03 105.97 142.89
Intensity Change Log 2 — -0.021247 0.311002 0.243007 — 0.086942 0.167289 0.213106 — 0.031107 0.244585 0.228895

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000685 0.000208 0.000426 0.002786 0.000234 0.000341 0.000111 0.000424 0.000521 0.000279 0.000151 0.000829
Bud Neck 0.008530 0.028866 0.026721 0.013024 0.054570 0.048554 0.016446 0.012577 0.025251 0.039372 0.017729 0.012653
Bud Site 0.023462 0.008390 0.031669 0.074288 0.033355 0.014218 0.021063 0.031920 0.027055 0.011500 0.022388 0.039176
Cell Periphery 0.000264 0.000091 0.000145 0.000175 0.000313 0.000188 0.000100 0.000172 0.000282 0.000143 0.000105 0.000172
Cytoplasm 0.386542* 0.442972* 0.259710* 0.314979* 0.275385* 0.385234* 0.496187* 0.473863* 0.346170* 0.412161* 0.466645* 0.446653*
Cytoplasmic Foci 0.066098 0.215391* 0.171111 0.114198 0.197701* 0.268337* 0.016012 0.060070 0.113896 0.243646* 0.035387 0.069340
Eisosomes 0.000074 0.000109 0.000074 0.000060 0.000196 0.000218 0.000023 0.000044 0.000118 0.000167 0.000029 0.000047
Endoplasmic Reticulum 0.000885 0.000596 0.002208 0.001917 0.001047 0.000520 0.000656 0.000992 0.000944 0.000555 0.000850 0.001151
Endosome 0.024603 0.015144 0.071501 0.028659 0.078641 0.025291 0.002322 0.011052 0.044229 0.020559 0.010964 0.014067
Golgi 0.004448 0.002907 0.001624 0.002276 0.003748 0.006010 0.000093 0.001071 0.004194 0.004563 0.000284 0.001277
Lipid Particles 0.002543 0.005616 0.003283 0.015591 0.006935 0.011861 0.000715 0.001387 0.004138 0.008949 0.001036 0.003819
Mitochondria 0.006433 0.005818 0.000459 0.002001 0.003066 0.016438 0.001255 0.001134 0.005210 0.011485 0.001156 0.001283
Mitotic Spindle 0.016285 0.005468 0.025020 0.062710 0.001051 0.006062 0.009815 0.020753 0.010752 0.005785 0.011714 0.027938
None 0.003621 0.003191 0.003553 0.009151 0.004765 0.002770 0.003497 0.005645 0.004036 0.002966 0.003504 0.006246
Nuclear Periphery 0.000982 0.000646 0.001203 0.000909 0.000221 0.000395 0.001528 0.001015 0.000705 0.000512 0.001488 0.000997
Nuclear Periphery Foci 0.000545 0.000424 0.018209 0.005723 0.000692 0.000235 0.000199 0.001071 0.000598 0.000323 0.002449 0.001868
Nucleolus 0.001433 0.001927 0.010653 0.000923 0.002866 0.002068 0.000856 0.001110 0.001954 0.002002 0.002080 0.001078
Nucleus 0.349746* 0.172210* 0.230438* 0.157911* 0.055075 0.121786 0.399051* 0.296882* 0.242722* 0.145301* 0.377987* 0.273082*
Peroxisomes 0.009002 0.011370 0.045174 0.064774 0.020892 0.019545 0.001270 0.015657 0.013320 0.015732 0.006755 0.024069
Vacuole 0.092058 0.077281 0.096347 0.127409 0.254786* 0.067981 0.028553 0.062145 0.151160 0.072318 0.037022 0.073322
Vacuole Periphery 0.001762 0.001374 0.000471 0.000538 0.004462 0.001950 0.000247 0.001014 0.002743 0.001682 0.000275 0.000933

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.98 -0.17 -2.85 -4.07 -2.87 -1.47 2.27 -3.11 -1.86 -5.23 2.10 3.36 -2.06 -4.66 -5.96
Bud Neck -8.66 -5.32 -2.09 5.75 3.94 1.10 7.27 8.04 11.23 1.75 -5.41 2.05 4.49 11.90 2.98
Bud Site 4.15 -3.01 -4.64 -6.88 -2.46 5.27 4.09 1.33 -5.64 -3.84 5.90 1.88 -2.47 -9.55 -4.83
Cell Periphery 3.73 2.15 2.00 -4.92 -0.38 2.79 5.27 4.02 2.58 -4.82 4.02 5.54 3.98 -0.22 -4.94
Cytoplasm -6.13 9.17 7.42 12.40 -1.30 -8.49 -14.01 -8.96 -0.71 6.45 -8.84 -9.90 -3.58 5.34 6.53
Cytoplasmic Foci -21.97 -7.37 -2.20 13.34 5.03 -6.45 19.75 12.90 30.19 -15.40 -21.77 17.18 7.49 33.09 -11.15
Eisosomes -5.49 -2.08 -0.23 4.26 1.66 -1.10 8.71 7.07 16.11 -14.23 -5.01 11.27 7.64 17.62 -11.81
Endoplasmic Reticulum 2.50 -7.32 -7.37 -8.63 -0.13 3.92 -0.22 -4.54 -9.58 -5.86 4.39 -3.48 -8.00 -12.68 -5.69
Endosome 3.17 -6.24 0.62 -1.97 6.43 9.08 13.22 11.17 6.28 -8.48 8.10 11.43 9.79 3.96 -2.79
Golgi 0.49 2.18 2.76 2.90 1.62 -2.50 7.18 5.04 6.50 -2.33 -1.51 6.10 4.56 6.88 -2.24
Lipid Particles -4.50 -3.48 -2.31 -1.15 -1.71 -4.24 6.88 5.20 12.13 -10.70 -7.69 8.32 1.53 8.31 -4.69
Mitochondria -0.53 3.12 2.70 6.31 -0.77 -8.21 2.61 2.94 10.37 1.49 -5.80 3.32 3.48 12.24 1.05
Mitotic Spindle 3.25 -3.47 -5.08 -6.35 -1.60 -3.49 -7.27 -10.46 -7.86 -6.17 2.24 -2.07 -7.64 -10.26 -6.43
None 0.01 -0.17 -1.10 -1.14 -1.08 1.21 0.82 -0.09 -3.89 -4.23 0.90 0.36 -1.41 -3.68 -3.80
Nuclear Periphery 6.05 -4.73 -4.44 -7.09 -0.13 -3.82 -28.21 -20.30 -16.77 3.80 6.00 -18.74 -12.95 -16.76 3.46
Nuclear Periphery Foci 1.46 -7.03 -6.49 -6.65 4.82 2.35 1.71 -4.67 -5.85 -5.61 3.23 -6.03 -7.16 -7.92 -0.52
Nucleolus 0.08 -4.79 0.43 0.42 4.84 1.30 1.62 -0.38 -2.43 -2.77 1.01 -2.44 -1.37 -2.44 1.10
Nucleus 15.50 7.42 13.28 2.10 3.94 -11.33 -52.17 -30.34 -23.47 15.28 11.72 -18.35 -3.64 -18.96 16.51
Peroxisomes -1.63 -5.95 -4.18 -3.75 0.73 0.54 8.20 2.02 2.12 -9.33 -1.77 4.65 -2.56 -1.39 -7.48
Vacuole 6.23 -0.19 -10.57 -13.71 -9.75 14.83 17.01 10.87 -9.31 -15.66 15.19 18.63 3.45 -14.08 -18.49
Vacuole Periphery 0.11 3.29 3.91 5.44 2.16 4.16 7.41 5.63 3.24 -1.11 2.79 8.35 4.72 3.31 -1.05
Standard name
Human Ortholog
Description Vacuolar alpha mannosidase; involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway
Localization
Cell Percentages vacuole (25%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ams1

Ams1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ams1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available